Research Topics
| Daniel SummererSummaryCountry: Germany Publications
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Detail Information
Publications
Enabling technologies of genomic-scale sequence enrichment for targeted high-throughput sequencingDaniel Summerer
febit biomed gmbh, Im Neuenheimer Feld 519, 69120 Heidelberg, Germany
Genomics 94:363-8. 2009..We summarize the different technologies and highlight individual characteristics related to general potential and different suitabilities for specific applications...
Microarray-based multicycle-enrichment of genomic subsets for targeted next-generation sequencingDaniel Summerer
febit biomed gmbh, 69120 Heidelberg, Germany
Genome Res 19:1616-21. 2009..Analysis of SNP calling accuracies after enrichment revealed excellent concordance, with the reference sequence closely mirroring the previously reported performance of Illumina sequencing conducted without sequence enrichment...
Targeted high throughput sequencing of a cancer-related exome subset by specific sequence capture with a fully automated microarray platformDaniel Summerer
febit biomed gmbh, Im Neuenheimer Feld 519, 69120 Heidelberg, Germany
Genomics 95:241-6. 2010..Uniformity was such that >68% of exons had at least half the median depth of coverage. An analysis of reference SNPs revealed a sensitivity of up to 93% and a specificity of 98.2% or higher...
A flexible and fully integrated system for amplification, detection and genotyping of genomic DNA targets based on microfluidic oligonucleotide arraysDaniel Summerer
Im Neuenheimer Feld, Heidelberg, Germany
N Biotechnol 27:149-55. 2010..The high automation grade of the system reduces contamination risk and workload and should enhance safety and reproducibility...
Targeted next-generation sequencing by specific capture of multiple genomic loci using low-volume microfluidic DNA arraysStephan Bau
febit biomed gmbh, Im Neuenheimer Feld 519, 69120, Heidelberg, Germany
Anal Bioanal Chem 393:171-5. 2009..6 and 107.5-fold for the individual target regions. This method represents a flexible and cost-effective approach for large-scale resequencing of complex genomes...
Specific sequence selection and next generation resequencing of 68 E. coli genes using HybSelectNadine Schracke
febit biomed gmbh, D 69120 Heidelberg, Germany
N Biotechnol 26:229-33. 2009..The study used Escherichia coli K12 MG1655 as a model organism to test parameters such as method fidelity, capacity or reproducibility as a proof-of-principle...
