Research Topics
| Jochen BuchsSummaryAffiliation: RWTH Aachen Country: Germany Publications
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Detail Information
Publications
Bioprocess control in microscale: scalable fermentations in disposable and user-friendly microfluidic systemsMatthias Funke
AVT Biochemical Engineering, RWTH Aachen University, Worringerweg 1, D 52074 Aachen, Germany
Microb Cell Fact 9:86. 2010..To realize a user-friendly system for routine laboratory work, disposable microfluidic MTPs are utilized which are actuated by a user-friendly pneumatic hardware...- Physiological relation between respiration activity and heterologous expression of selected benzoylformate decarboxylase variants in Escherichia coliThomas G Palmen
AVT Aachener Verfahrenstechnik, Biochemical Engineering, RWTH Aachen University, Worringerweg 1, D 52074 Aachen, Germany
Microb Cell Fact 9:76. 2010..The oxygen transfer rate (OTR) as parameter for growth and metabolic activity of the different E. coli clones was monitored on-line in LB, TB and modified PanG mineral medium with the Respiratory Activity MOnitoring System (RAMOS)... - Potential errors in conventional DOT measurement techniques in shake flasks and verification using a rotating flexitube optical sensorSven Hansen
AVT, Biochemical Engineering, RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany
BMC Biotechnol 11:49. 2011..The second principle aim is to use the newly built "flexitube optical sensor" to verify potential errors in conventional optical DOT measurements based on fixed sensor spots... - Controlling pH in shake flasks using polymer-based controlled-release discs with pre-determined release kineticsMarco Scheidle
Aachener Verfahrenstechnik Biochemical Engineering, RWTH Aachen University, Sammelbau Biologie, Worringer Weg 1, D 52074 Aachen, Germany
BMC Biotechnol 11:25. 2011..Since the sodium carbonate is gradually released from the discs in pre-determined kinetics, it will ultimately compensate the decrease in pH caused by the biological activity of microorganisms... - Replication methods and tools in high-throughput cultivation processes - recognizing potential variations of growth and product formation by on-line monitoringRobert Huber
AVT Aachener Verfahrenstechnik, Biochemical Engineering, RWTH Aachen University, Worringerweg 1, D 52074 Aachen, Germany
BMC Biotechnol 10:22. 2010..Finally, a high-throughput cultivation process was simulated with Corynebacterium glutamicum pEKEx2-phoD-GFP, beginning at the colony picking step... - How recombinant swollenin from Kluyveromyces lactis affects cellulosic substrates and accelerates their hydrolysisGernot Jäger
AVT Aachener Verfahrenstechnik, Biochemical Engineering, RWTH Aachen University, Worringerweg 1, D 52074 Aachen, Germany
Biotechnol Biofuels 4:33. 2011..abstract:.. - Validation of a high-throughput fermentation system based on online monitoring of biomass and fluorescence in continuously shaken microtiter platesFrank Kensy
AVT, Biochemical Engineering, RWTH Aachen University, Sammelbau Biologie, Worringerweg 1, 52074 Aachen, Germany
Microb Cell Fact 8:31. 2009..abstract:.. - Practical screening of purified cellobiohydrolases and endoglucanases with ?-cellulose and specification of hydrodynamicsGernot Jäger
AVT Aachener Verfahrenstechnik, Biochemical Engineering, RWTH Aachen University, Worringerweg 1, D 52074 Aachen, Germany
Biotechnol Biofuels 3:18. 2010..Moreover, this study investigates how hydrodynamics affects cellulase adsorption and activity onto ?-cellulose... - Microfluidic biolector-microfluidic bioprocess control in microtiter platesMatthias Funke
AVT Biochemical Engineering, RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany
Biotechnol Bioeng 107:497-505. 2010..This article elucidates this new concept and illustrates applications in fermentations of Escherichia coli under pH-controlled and fed-batch conditions in shaken MTPs... - Increased product formation induced by a directed secondary substrate limitation in a batch Hansenula polymorpha cultureKirsten Kottmeier
Chair of Biochemical Engineering, RWTH Aachen University, Worringerweg 1, Sammelbau Biologie, 52056 Aachen, Germany
Appl Microbiol Biotechnol 86:93-101. 2010..41-fold higher compared to unlimited conditions. A limitation of the substrate magnesium resulted in a 1.22-fold increase GFP formation in the case of glycerol as carbon source... - High cell density cultivation of recombinant yeasts and bacteria under non-pressurized and pressurized conditions in stirred tank bioreactorsArnd Knoll
Biochemical Engineering, RWTH Aachen University, 52056 Aachen, Germany
J Biotechnol 132:167-79. 2007..Furthermore, it was shown that increased carbon dioxide partial pressures did not remarkably inhibit the growth of the investigated model organisms... - An experimental comparison of respiration measuring techniques in fermenters and shake flasks: exhaust gas analyzer vs. RAMOS device vs. respirometerJuri M Seletzky
Biochemical Engineering, RWTH Aachen University, Sammelbau Biologie, Worringerweg 1, 52056, Aachen, Germany
J Ind Microbiol Biotechnol 34:123-30. 2007..If online monitoring is not compulsive, the respiration of the investigated microbial system extremely low, or the sample size small, a respirometer is recommended... - Scale-up from microtiter plate to laboratory fermenter: evaluation by online monitoring techniques of growth and protein expression in Escherichia coli and Hansenula polymorpha fermentationsFrank Kensy
AVT Biochemical Engineering, RWTH Aachen University, Sammelbau Biologie, Worringerweg 1, 52074 Aachen, Germany
Microb Cell Fact 8:68. 2009..4 L), two standard microbial expression systems, Escherichia coli and Hansenula polymorpha, were fermented in parallel at both scales and compared with regard to the biomass and protein formation... - Linear correlation between online capacitance and offline biomass measurement up to high cell densities in Escherichia coli fermentations in a pilot-scale pressurized bioreactorIngo Knabben
RWTH Aachen, AVT Biochemical Engineering, Worringer Weg 1, 52056 Aachen, Germany
J Microbiol Biotechnol 21:204-11. 2011..Consequently, online monitoring of biomass using a capacitance probe provides direct and fast information about the viable E. coli biomass generated under aerobic fermentation conditions at elevated headspace pressures... - The baffled microtiter plate: increased oxygen transfer and improved online monitoring in small scale fermentationsMatthias Funke
AVT Biochemical Engineering, RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany
Biotechnol Bioeng 103:1118-28. 2009.... - New method to determine the mass transfer resistance of sterile closures for shaken bioreactorsTibor Anderlei
Biochemical Engineering, RWTH Aachen University, Worringerweg 1, 52056 Aachen, Germany
Biotechnol Bioeng 98:999-1007. 2007.... - Propioin synthesis using thiamine diphosphate-dependent enzymesRenaud J Mikolajek
Biochemical Engineering, RWTH Aachen University, Worringer Weg 1, Aachen 52056, Germany
Biotechnol Prog 25:132-8. 2009..Both enzymes produce propioin with opposite enantiomeric excess: BAL produced the (S)-propioin (ee of 35%), whereas BFD yielded the (R)-enantiomer (ee of 67%)... - High-throughput screening of Hansenula polymorpha clones in the batch compared with the controlled-release fed-batch mode on a small scaleMarco Scheidle
Aachener Verfahrenstechnik Biochemical Engineering, RWTH Aachen University, Sammelbau Biologie, Aachen, Germany
FEMS Yeast Res 10:83-92. 2010..Ultimately, fed-batch screening is considerably better than screening in batch mode for fed-batch production processes with glucose as a carbon source... - Equalizing growth in high-throughput small scale cultivations via precultures operated in fed-batch modeRobert Huber
AVT Aachener Verfahrenstechnik, Biochemical Engineering, RWTH Aachen University, Worringerweg 1, D 52074 Aachen, Germany
Biotechnol Bioeng 103:1095-102. 2009..The growth of distinctly inoculated precultures in microtiter plates and shake flasks could be equalized for different microorganisms such as Escherichia coli and Hansenula polymorpha... - Substrate and water adsorption phenomena in a gas/solid enzymatic reactorKerasina Dimoula
RWTH Aachen University, Aachener Verfahrenstechnik Biochemical Engineering, Aachen, Germany
Biotechnol J 4:712-21. 2009..32, the secondary substrate, 2-propanol is not adsorbed at a detectable degree. The presence of water leads to a decrease of the adsorbed amount of acetophenone, by approximately 25%, at a water activity of 0.54... - Application of an improved continuous parallel shaken bioreactor system for three microbial model systemsAli Akgün
Biochemical Engineering, RWTH Aachen University, Sammelbau Biologie, Worringerweg 1, 52074 Aachen, Germany
Bioprocess Biosyst Eng 31:193-205. 2008..Essentially the same results were obtained in both types of bioreactors. However, many more reading points were obtained with the parallel shaken bioreactor system in the same time at much lower consumption of culture media... - Scale-up from shake flasks to fermenters in batch and continuous mode with Corynebacterium glutamicum on lactic acid based on oxygen transfer and pHJuri M Seletzky
Biochemical Engineering, RWTH Aachen University, Sammelbau Biologie, Worringerweg 1, 52056 Aachen, Germany
Biotechnol Bioeng 98:800-11. 2007..36 g/g) than in continuous cultures (Y(X/S) = 0.45 g/g). The experimental data (biomass, respiration, pH) could be described with a simple biological model combined with a mechanistic pH model... - Online determination of viable biomass up to very high cell densities in Arxula adeninivorans fermentations using an impedance signalIngo Knabben
RWTH Aachen, AVT Biochemical Engineering, Worringer Weg 1, 52056 Aachen, Germany
J Biotechnol 149:60-6. 2010..Unlike previous findings, in this study a linear correlation was found between capacitance and biomass up to concentrations of 174gL(-1) dry cell weight... - High-throughput screening for ionic liquids dissolving (ligno-)celluloseMichael Zavrel
Aachener Verfahrenstechnik Chair of Biochemical Engineering, RWTH Aachen University, Worringerweg 1, D 52074 Aachen, Germany
Bioresour Technol 100:2580-7. 2009..The screening results indicate that among the ionic liquids tested EMIM Ac is the most efficient for dissolving cellulose. Moreover, it was observed that AMIM Cl is the most effective ionic liquid for dissolving wood chips... - Acetate-glycerol cometabolism: cultivating Schizosaccharomyces pombe on a non-fermentable carbon source in a defined minimal mediumTobias Klement
AVT Biochemical Engineering, RWTH Aachen University, Worringer Weg, D 52074 Aachen, Germany
J Biosci Bioeng 112:20-5. 2011..S. pombe showed optimal respiration activity, growth, and product formation by co-utilizing 20g/L glycerol and 2.5g/L sodium acetate... - High cell-density processes in batch mode of a genetically engineered Escherichia coli strain with minimized overflow metabolism using a pressurized bioreactorIngo Knabben
RWTH Aachen, AVT Biochemical Engineering, Worringer Weg 1, 52056 Aachen, Germany
J Biotechnol 150:73-9. 2010..Consequentially, to our knowledge, this study using a bioreactor with elevated headspace pressure generate the highest oxygen transfer rate (451 mmol L(-1) h(-1)) ever reached in batch cultivations... - Utilizing high-throughput experimentation to enhance specific productivity of an E.coli T7 expression system by phosphate limitationRobert Huber
RWTH Aachen University, Worringerweg 1, D 52074 Aachen, Germany
BMC Biotechnol 11:22. 2011.... - Screening for enzyme activity in turbid suspensions with scattered lightRobert Huber
AVT Aachener Verfahrenstechnik, Biochemical Engineering, RWTH Aachen University, Worringerweg 1, D 52074 Aachen, Germany
Biotechnol Prog 27:555-61. 2011..Moreover, this method can be applied to other enzyme systems that catalyze various reactions such as cellulose decomposition... - An activity, stability and selectivity comparison of propioin synthesis by thiamine diphosphate-dependent enzymes in a solid/gas bioreactorRenaud Mikolajek
Biochemical Engineering, RWTH Aachen University, Worringer Weg 1, 52056 Aachen, Germany
Chembiochem 8:1063-70. 2007..Unexpectedly, the stereoselectivity of both enzymes (ee of 19 % for BAL and racemic mixture for BFD) was significantly impaired in the gas phase compared to the liquid phase... - Effect of elevated dissolved carbon dioxide concentrations on growth of Corynebacterium glutamicum on D-glucose and L-lactateCarsten Bäumchen
Biochemical Engineering, RWTH Aachen University, D 52074 Aachen, Germany
J Biotechnol 128:868-74. 2007..37 h(-1) under aeration with air to a maximum value of 0.47 h(-1) at a pCO2 of 0.26 bar. At very high pCO2 (0.81 bar) mu(max) decreased down to 0.35 h(-1) independent of the carbon source... - Optimization of enzymatic gas-phase reactions by increasing the long-term stability of the catalystClara Ferloni
Biochemical Engineering, RWTH Aachen University, 52056 Aachen, Germany
Biotechnol Prog 20:975-8. 2004..The stabilized catalyst preparation was employed in a continuous gas-phase reactor at different temperatures (25-60 degrees C). At 50 degrees C, a space-time yield of 107 g/L/d was achieved within the first 80 h of continuous reaction... - Process development in Hansenula polymorpha and Arxula adeninivorans, a re-assessmentChristoph Stöckmann
PharmedArtis GmbH, Forckenbeckstr, 6, 52074 Aachen, Germany
Microb Cell Fact 8:22. 2009..polymorpha provided a suitable basis for the initial definition of fermentation conditions for this dimorphic yeast. Characteristics like osmo- and thermotolerance can be addressed for the definition of culture conditions... - Robo-Lector - a novel platform for automated high-throughput cultivations in microtiter plates with high information contentRobert Huber
AVT, Biochemical Engineering, RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany
Microb Cell Fact 8:42. 2009..abstract:.. - Asymmetric division of Hansenula polymorpha reflected by a drop of light scatter intensity measured in batch microtiter plate cultivations at phosphate limitationKirsten Kottmeier
Biochemical Engineering, Aachen University of Technology, 52056 Aachen, Germany
Biotechnol Bioeng 104:554-61. 2009..The formation of the subpopulations may be caused by an increased asymmetry in the cell cycle due to phosphate limitation... - New insights in the spatially resolved dynamic pH measurement in macroscopic large absorbent particles by confocal laser scanning microscopyMatthias Heinemann
Biochemical Engineering, RWTH Aachen University, Worringer Weg 1, 52056 Aachen, Germany
J Chromatogr A 1024:45-53. 2004..The resulting consequences for further development and application of CLSM based techniques to measure pH in macroscopic large absorbent particles are illustrated and discussed... - Mechanistic kinetic model for symmetric carboligations using benzaldehyde lyaseMichael Zavrel
Biochemical Engineering, RWTH Aachen University, Worringerweg 1, D 52074 Aachen, Germany
Biotechnol Bioeng 101:27-38. 2008..It is shown that this approach leads to a robust kinetic model with accurate parameter estimates and an excellent prediction capability... - Assaying CO2 release for determination of formate dehydrogenase activity in entrapment matrices and aqueous-organic two-phase systemsMarion B Ansorge-Schumacher
Department of Biotechnology, RWTH Aachen University, D 52056 Aachen, Germany
Biotechnol Bioeng 95:199-203. 2006.... - Transponder-based sensor for monitoring electrical properties of biological cell solutionsMirko C Hofmann
Institute of Materials in Electrical Engineering, RWTH Aachen University, Sommerfeldstr 24, D 52074 Aachen, Germany
J Biosci Bioeng 100:172-7. 2005..The performed measurements show the expected correlation between the determined permittivity and optical density. The solution under test is a yeast culture in YPG medium... - Screening of cellulases for biofuel production: online monitoring of the enzymatic hydrolysis of insoluble cellulose using high-throughput scattered light detectionGernot Jäger
AVT Aachener Verfahrenstechnik, Biochemical Engineering, RWTH Aachen University, Aachen, Germany
Biotechnol J 6:74-85. 2011..It will considerably simplify and accelerate fundamental research on cellulase screening... - Modelling and advanced understanding of unsteady-state gas transfer in shaking bioreactorsGhassem Amoabediny
Biochemical Engineering, RWTH Aachen University, Worringerweg 1, Sammelbau Biologie, D 52056 Aachen, Federal Republic of Germany
Biotechnol Appl Biochem 46:57-67. 2007..A good agreement between the introduced unsteady-state model and experimental results for the sulfite oxidation as a chemical model system confirmed the validity and usefulness of the proposed unsteady-state approach... - "Enzyme Test Bench," a high-throughput enzyme characterization technique including the long-term stabilityKirill Rachinskiy
Department of Biochemical Engineering, Sammelbau Biologie, RWTH Aachen University, D 52074 Aachen, Germany
Biotechnol Bioeng 103:305-22. 2009..Thus, temperature dependent enzyme characterization is essential in primary screening to predict its long term behavior... - Effect of oxygen limitation and medium composition on Escherichia coli fermentation in shake-flask culturesMario Losen
Biochemical Engineering, RWTH Aachen University, Germany, and Institute of Enzyme Technology, , Germany
Biotechnol Prog 20:1062-8. 2004..This example demonstrates how variation of medium composition and oxygen supply can be evaluated by the measurement of the respiratory activity. This can help to efficiently optimize screening conditions for E. coli... - The oxygen mass transfer, carbon dioxide inhibition, heat removal, and the energy and cost efficiencies of high pressure fermentationArnd Knoll
Biochemical Engineering, RWTH Aachen University, Worringer Weg 1, 52056 Aachen, Germany
Adv Biochem Eng Biotechnol 92:77-99. 2005..6 bar... - Study on mesophilic and thermophilic alcohol dehydrogenases in gas-phase reactionArchana H Trivedi
Biochemical Engineering, RWTH Aachen University, Worringerweg 1, D-52056 Aachen, Germany
Biotechnol Prog 22:454-8. 2006..2 million for LBADH and 1.7 million for ADH T, respectively. Interestingly, the ADH from the mesophilic L. brevisshowed the higher thermostability in the nonconventional medium gas phase... - Optimization of adsorptive immobilization of alcohol dehydrogenasesArchana Trivedi
Biochemical Engineering, RWTH Aachen University, Worringerweg 1, Sammelbau Biologie, 52056 Aachen, Germany
J Biosci Bioeng 99:340-7. 2005..ADH from Thermoanaerobacter species (ADH T) is found to be stable under drying conditions (>15 kPa) in contrast to LBADH and YADH... - Characterization of gas-liquid mass transfer phenomena in microtiter platesRobert Hermann
Department of Biochemical Engineering, Aachen University of Technology, Sammelbau Biologie, Worringerweg 1, D-52056 Aachen, Germany
Biotechnol Bioeng 81:178-86. 2003..In square-shaped microtiter plate wells the corners act as baffles and cause a significant increase of OTR(max), a, and k(L). An OTR(max) of up to 0.15 mol/L/h can be reached in square-shaped wells... - Hydromechanical stress in shake flasks: correlation for the maximum local energy dissipation rateCyril P Peter
Biochemical Engineering, RWTH Aachen University, Worringerweg 1, 52056 Aachen, Germany
Biotechnol Bioeng 93:1164-76. 2006..Non-negligible drop viscosity may be considered by known literature correlations. Further, from dispersion experiments a critical Reynolds number of about 60,000 is proposed for turbulent flow in unbaffled shake flasks... - Oxygen transfer phenomena in 48-well microtiter plates: determination by optical monitoring of sulfite oxidation and verification by real-time measurement during microbial growthFrank Kensy
Biochemical Engineering, RWTH Aachen University, Sammelbau Biologie, Worringerweg 1, D-52074 Aachen, Germany
Biotechnol Bioeng 89:698-708. 2005..The 48-well plates emerge, therefore, as an excellent alternative for microbial cultivation and expression studies combining the advantages of both the high-throughput 96-well MTP and the classical shaken Erlenmeyer flask... - Metabolic activity of Corynebacterium glutamicum grown on L: -lactic acid under stressJuri M Seletzky
Biochemical Engineering, RWTH Aachen University, Sammelbau Biologie, Worringerweg 1, 52056 Aachen, Germany
Appl Microbiol Biotechnol 72:1297-307. 2006..Without adaptation, the organism did not grow at pH< or =5 or > or =9... - Method for quantitative determination of spatial polymer distribution in alginate beads using Raman spectroscopyMatthias Heinemann
RWTH Aachen University, Biochemical Engineering, 52056 Aachen, Germany
Appl Spectrosc 59:280-5. 2005....