Kai Stühler

Summary

Affiliation: Ruhr-University Bochum
Country: Germany

Publications

  1. ncbi Pilot study of the Human Proteome Organisation Brain Proteome Project: applying different 2-DE techniques to monitor proteomic changes during murine brain development
    Kai Stühler
    Medizinisches Proteom Center, Ruhr University Bochum, Bochum, Germany
    Proteomics 6:4899-913. 2006
  2. ncbi Simultaneous extraction of nucleic acids and proteins from tissue specimens by ultracentrifugation: A protocol using the high-salt protein fraction for quantitative proteome analysis
    Michael Grzendowski
    Medizinisches Proteom Center, Ruhr Universitat Bochum, Bochum, Germany
    Proteomics 9:4985-90. 2009
  3. ncbi Immunoscreening of the extracellular proteome of colorectal cancer cells
    Susanne Klein-Scory
    Department of Internal Medicine, Knappschaftskrankenhaus, IMBL, Ruhr University Bochum, Bochum, Germany
    BMC Cancer 10:70. 2010
  4. ncbi Keratin 23, a novel DPC4/Smad4 target gene which binds 14-3-3?
    Sven T Liffers
    Medizinisches Proteom Center, Ruhr University Bochum Zentrum fuer Klinische Forschung, Universitaetsstr 150, 44780 Bochum, Germany
    BMC Cancer 11:137. 2011
  5. ncbi Application of fluorescence dye saturation labeling for differential proteome analysis of 1,000 microdissected cells from pancreatic ductal adenocarcinoma precursor lesions
    Barbara Sitek
    Ruhr University Bochum, Bochum, Germany
    Methods Mol Biol 425:1-14. 2008
  6. ncbi Identification of proteomic differences between squamous cell carcinoma of the lung and bronchial epithelium
    Gereon Poschmann
    Medizinisches Proteom Center, Ruhr Universitat Bochum, 44801 Bochum, Germany
    Mol Cell Proteomics 8:1105-16. 2009
  7. ncbi Differential proteome analysis of human gliomas stratified for loss of heterozygosity on chromosomal arms 1p and 19q
    Michael Grzendowski
    Medizinisches Proteom Center, Ruhr Universitat Bochum, Universitatsstrasse 150, 44801 Bochum, Germany
    Neuro Oncol 12:243-56. 2010
  8. ncbi Analysis of the pancreatic tumor progression by a quantitative proteomic approach and immunhistochemical validation
    Barbara Sitek
    Medizinisches Proteom Center, Ruhr Universitat Bochum, Germany
    J Proteome Res 8:1647-56. 2009
  9. ncbi New insight into stimulus-induced plasticity of the olfactory epithelium in Mus musculus by quantitative proteomics
    Jon Barbour
    Medizinisches Proteom Center, Ruhr University Bochum, Universitatsstrasse 150, 44780 Bochum, Germany
    J Proteome Res 7:1594-605. 2008
  10. ncbi A catalogue of proteins released by colorectal cancer cells in vitro as an alternative source for biomarker discovery
    Hanna C Diehl
    Medical Proteome Center, University of Bochum, Bochum, Germany Department of Internal Medicine, IMBL, Knappschaftskrankenhaus, University of Bochum, Bochum, Germany
    Proteomics Clin Appl 1:47-61. 2007

Collaborators

Detail Information

Publications16

  1. ncbi Pilot study of the Human Proteome Organisation Brain Proteome Project: applying different 2-DE techniques to monitor proteomic changes during murine brain development
    Kai Stühler
    Medizinisches Proteom Center, Ruhr University Bochum, Bochum, Germany
    Proteomics 6:4899-913. 2006
    ....
  2. ncbi Simultaneous extraction of nucleic acids and proteins from tissue specimens by ultracentrifugation: A protocol using the high-salt protein fraction for quantitative proteome analysis
    Michael Grzendowski
    Medizinisches Proteom Center, Ruhr Universitat Bochum, Bochum, Germany
    Proteomics 9:4985-90. 2009
    ..We applied this method to extracts from primary human brain tumors and demonstrate its successful application for protein expression profiling in these tumors using 2-D DIGE, MS and Western blotting...
  3. ncbi Immunoscreening of the extracellular proteome of colorectal cancer cells
    Susanne Klein-Scory
    Department of Internal Medicine, Knappschaftskrankenhaus, IMBL, Ruhr University Bochum, Bochum, Germany
    BMC Cancer 10:70. 2010
    ..Aiming at the latter approach we here have implemented an autoantibody profiling strategy which particularly focuses on proteins released by tumor cells in vitro: the so-called secretome...
  4. ncbi Keratin 23, a novel DPC4/Smad4 target gene which binds 14-3-3?
    Sven T Liffers
    Medizinisches Proteom Center, Ruhr University Bochum Zentrum fuer Klinische Forschung, Universitaetsstr 150, 44780 Bochum, Germany
    BMC Cancer 11:137. 2011
    ..Using this cellular model system, we searched for new Smad4 targets comparing nuclear subproteomes derived from Smad4 re-expressing and Smad4 negative SW480 cells...
  5. ncbi Application of fluorescence dye saturation labeling for differential proteome analysis of 1,000 microdissected cells from pancreatic ductal adenocarcinoma precursor lesions
    Barbara Sitek
    Ruhr University Bochum, Bochum, Germany
    Methods Mol Biol 425:1-14. 2008
    ..Here, we present a proteomics approach allowing us to analyse 1,000 microdissected cells stemming from pancreatic carcinoma precursor lesions applying fluorescence dye saturation labeling...
  6. ncbi Identification of proteomic differences between squamous cell carcinoma of the lung and bronchial epithelium
    Gereon Poschmann
    Medizinisches Proteom Center, Ruhr Universitat Bochum, 44801 Bochum, Germany
    Mol Cell Proteomics 8:1105-16. 2009
    ..Therefore this protein might be used as a marker for stratification between different tumor grades...
  7. ncbi Differential proteome analysis of human gliomas stratified for loss of heterozygosity on chromosomal arms 1p and 19q
    Michael Grzendowski
    Medizinisches Proteom Center, Ruhr Universitat Bochum, Universitatsstrasse 150, 44801 Bochum, Germany
    Neuro Oncol 12:243-56. 2010
    ..In addition, our data support the hypothesis that 1p/19q-deleted gliomas frequently show epigenetic down-regulation of multiple genes due to aberrant methylation of the 5'-CpG islands...
  8. ncbi Analysis of the pancreatic tumor progression by a quantitative proteomic approach and immunhistochemical validation
    Barbara Sitek
    Medizinisches Proteom Center, Ruhr Universitat Bochum, Germany
    J Proteome Res 8:1647-56. 2009
    ....
  9. ncbi New insight into stimulus-induced plasticity of the olfactory epithelium in Mus musculus by quantitative proteomics
    Jon Barbour
    Medizinisches Proteom Center, Ruhr University Bochum, Universitatsstrasse 150, 44780 Bochum, Germany
    J Proteome Res 7:1594-605. 2008
    ..Among these proteins are different lipocalins, which seem to play a crucial role in the regulation of the sensitivity of the olfactory system...
  10. ncbi A catalogue of proteins released by colorectal cancer cells in vitro as an alternative source for biomarker discovery
    Hanna C Diehl
    Medical Proteome Center, University of Bochum, Bochum, Germany Department of Internal Medicine, IMBL, Knappschaftskrankenhaus, University of Bochum, Bochum, Germany
    Proteomics Clin Appl 1:47-61. 2007
    ....
  11. ncbi Proteomic analysis of astroglial connexin43 silencing uncovers a cytoskeletal platform involved in process formation and migration
    Stephan Olk
    Department of Neuroanatomy and Molecular Brain Research, Ruhr University Bochum, Germany
    Glia 58:494-505. 2010
    ..Our results provide mechanistic clues for an understanding of Cx43 interaction with cellular motor activities such as migration and process formation in astrocytes...
  12. ncbi Unintended spread of a biosafety level 2 recombinant retrovirus
    Alexander Stang
    Department of Molecular and Medical Virology, Ruhr University Bochum, D 44780 Bochum, Germany
    Retrovirology 6:86. 2009
    ..Therefore, we explored two novel methods to identify viral contaminations in cell lines without prior knowledge of the kind of contaminant...
  13. ncbi HUPO Brain Proteome Project: summary of the pilot phase and introduction of a comprehensive data reprocessing strategy
    Michael Hamacher
    Medizinisches Proteom Center, Ruhr Universitat Bochum, Bochum, Germany
    Proteomics 6:4890-8. 2006
    ..In the following, results of the single groups as well as the centralised reprocessing effort will be summarised and compared, showing the added value of this concerted work...
  14. ncbi Study of early leaf senescence in Arabidopsis thaliana by quantitative proteomics using reciprocal 14N/15N labeling and difference gel electrophoresis
    Romano Hebeler
    Medizinisches Proteom Center, Zentrum fuer klinische Forschung, Ruhr Universitaet Bochum, Universitaetsstrasse 150, 44780 Bochum, Germany
    Mol Cell Proteomics 7:108-20. 2008
    ..With the exception of one glutathione S-transferase isoform, none of these proteins has been linked to leaf senescence before...
  15. ncbi High-performance proteomics as a tool in biomarker discovery
    Helmut E Meyer
    Medizinisches Proteom Center, Ruhr University Bochum, Bochum, Germany
    Proteomics 7:18-26. 2007
    ....
  16. ncbi Differential proteome analysis of colon carcinoma cell line SW480 after reconstitution of the tumour suppressor Smad4
    Kai Stühler
    Medizinisches Proteom Center, Ruhr University Bochum, ZKF E 1 43, Universitatsstrasse 150, 44801, Bochum, Germany
    Anal Bioanal Chem 386:1603-12. 2006
    ..From the functions of the candidate proteins we obtained new insights into Smad4's participation in processes, for example apoptosis, differentiation, and proliferation...