Research Topics
| J DennerSummaryAffiliation: Robert Koch-Institut Country: Germany Publications
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Detail Information
Publications
Infection barriers to successful xenotransplantation focusing on porcine endogenous retrovirusesJoachim Denner
Address correspondence to Joachim Denner
Clin Microbiol Rev 25:318-43. 2012....- Lack of evidence for xenotropic murine leukemia virus-related virus(XMRV) in German prostate cancer patientsOliver Hohn
Robert Koch Institute, Centre for Biological Safety 4, Nordufer 20, 13353 Berlin, Germany
Retrovirology 6:92. 2009..Here, we attempt to measure the prevalence of XMRV in prostate cancer cases in Germany and determine whether an analogous association with the R462Q polymorphism exists... - Genetic alterations of the long terminal repeat of an ecotropic porcine endogenous retrovirus during passage in human cellsJoachim Denner
Robert Koch Institute, Nordufer 20, D 13353 Berlin, Germany
Virology 314:125-33. 2003..These data indicate that recombinant PERVs generated during infection of human cells can adapt and subsequently replicate with greater efficiency... - Transspecies transmissions of retroviruses: new casesJoachim Denner
Robert Koch Institute, 13353 Berlin, Germany
Virology 369:229-33. 2007..Studies on newly reported natural and experimental transspecies transmissions of the koala retrovirus (KoRV) may help to understand such events... - [Report on the 7th Xenotransplantation Mini-Symposium at the Robert Koch Institute, Berlin, June 2004]J Denner
Robert Koch-Institut, Berlin
Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz 47:1014-7. 2004 - No transmission of porcine endogenous retroviruses (PERVs) in a long-term pig to rat xenotransplantation model and no infection of immunosuppressed ratsJoachim Denner
Robert Koch Institute, Berlin, Germany
Ann Transplant 13:20-31. 2008..However, it remains unclear whether PERVs infect transplant recipients in vivo and, if so, whether they are pathogenic. It is therefore essential to perform in vivo infection studies in animal models... - Porcine endogenous retroviruses (PERVs) and xenotransplantation: screening for transmission in several clinical trials and in experimental models using non-human primatesJ Denner
Robert Koch Institute, Berlin, Germany
Ann Transplant 8:39-48. 2003..These data are crucial for the evaluation of xenotransplantation safety because they demonstrate that PERVs cannot easily be transmitted to other species including man... - Recombinant porcine endogenous retroviruses (PERV-A/C): a new risk for xenotransplantation?Joachim Denner
Robert Koch Institute, Nordufer 20, Berlin, Germany
Arch Virol 153:1421-6. 2008..This review compares recombinant PERVs with other recombinant retroviruses in order to evaluate their potential pathogenicity... - The International Xenotransplantation Association consensus statement on conditions for undertaking clinical trials of porcine islet products in type 1 diabetes--chapter 5: Strategies to prevent transmission of porcine endogenous retrovirusesJoachim Denner
Robert Koch Institute, Berlin, Germany
Xenotransplantation 16:239-48. 2009..Finally, virus safety of xenotransplantation is a fast-developing field, and new experimental findings will change existing strategies and introduce new ones... - Detection of a gammaretrovirus, XMRV, in the human population: open questions and implications for xenotransplantationJoachim Denner
Retrovirus Induced Immunosuppression, Robert Koch Institute, Nordufer 20, D 13353 Berlin, Germany
Retrovirology 7:16. 2010..Since recombination can occur between viruses, and recombinants can exhibit synergism, recipients should be tested for XMRV before xenotransplantation... - Towards an AIDS vaccine: the transmembrane envelope protein as target for broadly neutralizing antibodiesJoachim Denner
Robert Koch Institute, Berlin, Germany
Hum Vaccin 7:4-9. 2011..These data may help to design antigens able to induce specific broadly neutralizing antibodies... - Infectious risk in xenotransplantation--what post-transplant screening for the human recipient?Joachim Denner
Robert Koch Institute, Berlin, Germany
Xenotransplantation 18:151-7. 2011..Until now, no PERV transmission was observed in experimental and clinical xenotransplantations as well as in numerous infection experiments. Nevertheless, several strategies have been developed to prevent PERV transmission... - Is porcine endogenous retrovirus (PERV) transmission still relevant?J Denner
Robert Koch Institute, Berlin, Germany
Transplant Proc 40:587-9. 2008..Intensive work is required to improve and to combine these strategies to further decrease the putative risk of PERV transmission following xenotransplantation... - Virus safety in xenotransplantation: first exploratory in vivo studies in small laboratory animals and non-human primatesV Specke
Robert Koch-Institute, Berlin, Germany
Transpl Immunol 9:281-8. 2002..This apparent difference in the outcome of the in vitro and the in vivo data might be explained by an efficient elimination of the virus by the innate or adaptive immunity of the animals... - Porcine endogenous retroviruses: in vitro host range and attempts to establish small animal modelsV Specke
Robert Koch-Institut, Nordufer 20, D-13353 Berlin, Germany Paul-Ehrlich-Institut, Paul-Ehrlich-Strasse 51-59, D-63225 Langen, Germany
J Gen Virol 82:837-44. 2001..No antibodies against PERV and no provirus integration were observed in any of the treated animals. This suggests that productive infection of these animals did not occur in this experimental setting... - Absence of infection in pigs inoculated with high-titre recombinant PERV-A/CD Kaulitz
Robert Koch Institute, Berlin, Germany
Arch Virol 156:707-10. 2011..No provirus integration was found in blood cells or in various tissues, and no antibody production was observed, indicating the absence of infection... - A neutralization assay for HIV-2 based on measurement of provirus integration by duplex real-time PCRR Behrendt
Robert Koch Institute, Nordufer 20, D 13353 Berlin, Germany
J Virol Methods 159:40-6. 2009.... - [Porcine endogenous retroviruses (PERVs) and xenotransplantation. A risk for the recipient and for society?]V Specke
Robert Koch-Institut, Berlin
Dtsch Med Wochenschr 128:1301-6. 2003 - No evidence for PERV release by islet cells from German landrace pigsMarkus Irgang
Robert Koch Institute, Berlin, Germany
Ann Transplant 13:59-66. 2008.... - Neutralizing antibodies against conserved domains of p15E of porcine endogenous retroviruses: basis for a vaccine for xenotransplantation?Uwe Fiebig
Robert Koch-Institute, Nordufer 20, D-13353 Berlin, Germany
Virology 307:406-13. 2003..These domains of the transmembrane proteins of different retroviruses are an effective target for neutralizing antibodies and may be a useful antigen to create an antiretroviral vaccine... - Productive infection of human primary cells and cell lines with porcine endogenous retrovirusesV Specke
Robert Koch-Institute, Nordufer 20, Berlin, D-13353, Germany
Virology 285:177-80. 2001..Infection with PERVs was shown for human peripheral blood mononuclear cells, primary endothelial cells, and primary aortic smooth muscle cells as well as lymphocytic, monocytic, and epithelial cell lines... - Inhibition of porcine endogenous retroviruses (PERVs) in primary porcine cells by RNA interference using lentiviral vectorsB Dieckhoff
Robert Koch Institute, Berlin, Germany
Arch Virol 152:629-34. 2007..Thus, lentiviral shRNA vectors could be used to knockdown PERV expression and create transgenic pigs with a reduced risk of PERV transmission during xenotransplantation... - Neutralising antibodies against the transmembrane protein of feline leukaemia virus (FeLV)S Langhammer
Robert Koch Institute, Berlin, Germany
Vaccine 23:3341-8. 2005..One of the epitopes has partial homology and both are located in regions corresponding to epitopes recognised by neutralising antibodies in patients infected with HIV-1... - Porcine endogenous retroviruses: no infection in patients treated with a bioreactor based on porcine liver cellsMarkus Irgang
Robert Koch Institute, Nordufer 20, D-13353 Berlin, Germany
J Clin Virol 28:141-54. 2003..The data indicate that no PERV infection had occurred in none of the patients treated with the CellModule bioreactor containing porcine cells... - Characterisation of a human cell-adapted porcine endogenous retrovirus PERV-A/CAlexander Karlas
Robert Koch Institute, Berlin, Germany
Ann Transplant 15:45-54. 2010..In contrast to the LTR, the sequence of the env gene did not change, indicating that the LTR represents the determinant of high infectivity... - Porcine endogenous retroviruses PERV-A and PERV-B infect neither mouse cells in vitro nor SCID mice in vivoMarkus Irgang
Robert Koch-Institute, Berlin, Germany
Intervirology 48:167-73. 2005.... - Expression of porcine endogenous retroviruses (PERVs) in melanomas of Munich miniature swine (MMS) TrollBritta Dieckhoff
Robert Koch Institute, Nordufer 20, 13353 Berlin, Germany
Vet Microbiol 123:53-68. 2007..Genomic RNA of PERV-A, -B and -C were found in pelleted virus particles. Although PERV expression was elevated in melanomas and pulmonary metastasis-derived cell cultures, the function of the virus in tumour development is still unclear... - Mode of interaction between the HIV-1-neutralizing monoclonal antibody 2F5 and its epitopeUwe Fiebig
Robert Koch Institute, Nordufer 20, Berlin D 13353, Germany
AIDS 23:887-95. 2009..Knowledge of these interactions would be useful for the design of antigens used to induce 2F5-like antibodies... - No in vivo infection of triple immunosuppressed non-human primates after inoculation with high titers of porcine endogenous retrovirusesVolker Specke
Robert Koch Institute, Berlin, Germany
Xenotransplantation 16:34-44. 2009..Absence of PERV transmission has been shown in first experimental and clinical xenotransplantations; however, these trials suffered from the absence of long-term exposure (transplant survival) and profound immunosuppression... - Induction of antibodies binding to the membrane proximal external region of gp36 of HIV-2R Behrendt
Robert Koch Institute, Berlin, Germany
Intervirology 55:252-6. 2012..In individuals infected with HIV-2 such antibodies were not yet reported... - Productive infection of a mink cell line with porcine endogenous retroviruses (PERVs) but lack of transmission to minks in vivoV Specke
Robert Koch-Institut, Berlin, Germany
Arch Virol 147:305-19. 2002..Neither antibody production against PERV nor integration of viral DNA or production of viral proteins in tissues of different organs could be detected 12 weeks post virus inoculation, indicating that PERV infection had not occurred... - Transspecies transmission of the endogenous koala retrovirusUwe Fiebig
Robert Koch Institute, Nordufer 20, D 13353 Berlin, Germany
J Virol 80:5651-4. 2006..Finally, evidence for immunosuppressive properties of the KoRV was obtained... - Antibodies neutralizing feline leukaemia virus (FeLV) in cats immunized with the transmembrane envelope protein p15EStefan Langhammer
Robert Koch Institute, Berlin, Germany
Immunology 117:229-37. 2006..Since homologous epitope sequences are present in feline endogenous retroviruses, it appears that tolerance against these sequences is not induced... - Regulation of human endogenous retrovirus-K expression in melanomas by CpG methylationSven Stengel
Retrovirus Induced Immunosuppression, Robert Koch Institute, Nordufer 20, Berlin, Germany
Genes Chromosomes Cancer 49:401-11. 2010..These results demonstrate that increased HERV-K expression in melanomas may be due to increased promoter activity and demethylation of the 5'LTR... - Knockdown of porcine endogenous retrovirus (PERV) expression by PERV-specific shRNA in transgenic pigsBritta Dieckhoff
Robert Koch Institute, Berlin, Germany
Xenotransplantation 15:36-45. 2008..RNA interference is a promising option to inhibit PERV transmission... - Response to editors' letter in Xenotransplantation on manuscripts on porcine endogenous retrovirusesJoachim Denner
Robert Koch-Institut, Berlin, Germany
Xenotransplantation 13:282-3. 2006 - Distribution and expression of porcine endogenous retroviruses in multi-transgenic pigs generated for xenotransplantationBritta Dieckhoff
Robert Koch Institute, Nordufer 20, Berlin, Germany
Xenotransplantation 16:64-73. 2009..The production of transgenic pigs may also be associated with the integration of the transgene adjacent to or into the locus of a PERV provirus, potentially leading to an enhanced virus expression... - Clinical extracorporeal hybrid liver support--phase I study with primary porcine liver cellsI M Sauer
General, Visceral and Transplantation Surgery, Charite Campus Virchow Klinikum, Humboldt University Berlin, Berlin, Germany
Xenotransplantation 10:460-9. 2003..The bioreactor technology has been integrated into a modular extracorporeal liver support (MELS) system, combining biologic liver support with artificial detoxification technology... - Absence of transmission of potentially xenotic viruses in a prospective pig to primate islet xenotransplantation studyOlga Garkavenko
Molecular Diagnostic Laboratory, Living Cell Technologies NZ Ltd, Auckland, New Zealand
J Med Virol 80:2046-52. 2008..There was no evidence of pig virus transmission into primate recipients. This preclinical study underlines the information concerning viral safety of islet cell xenograft in pig-to-primate xenotransplantation... - Differences in release and determination of subtype of porcine endogenous retroviruses produced by stimulated normal pig blood cellsStefan J Tacke
Paul Ehrlich Institute, Langen, Germany
Intervirology 46:17-24. 2003..CONCLUSION: The data suggest that the PBMCs transmitted within a xenotransplant may release PERV. These data also suggest that pig strains producing low amounts of virus could be more suitable for xenotransplantation... - Absence of PERV specific humoral immune response in baboons after transplantation of porcine cells or organsUlrich Martin
Leibniz Research Laboratories for Biotechnology and Artificial Organs, Department of Thoracic and Cardiovascular Surgery, Hannover Medical School, Podbielskistrasse 380, 30659 Hannover, Germany
Transpl Int 15:361-8. 2002..Our data suggest that no productive PERV infection and no continuous PERV release takes place in the nonhuman primates analyzed in this study... - No evidence for infection of human embryonic stem cells by feeder cell-derived murine leukemia virusesMichal Amit
Department of Obstetrics and Gynecology, Rambam Medical Center, Faculty of Medicine, The Technion, Haifa, Israel
Stem Cells 23:761-71. 2005..Our results suggest that recently established hESC lines are free of MuLV infections despite long-term close contact with MEFs... - Inhibition of porcine endogenous retroviruses by RNA interference: increasing the safety of xenotransplantationAlexander Karlas
Robert Koch-Institute, D-13353 Berlin, Germany
Virology 325:18-23. 2004..Cells or organs from transgenic pigs producing such shRNAs should increase the safety of xenotransplantation... - Analysis of pig-to-human porcine endogenous retrovirus transmission in a triple-species kidney xenotransplantation modelMonica E Winkler
Leibniz Research Laboratories for Biotechnology and Artificial Organs LEBAO, Hannover, Germany
Transpl Int 17:848-58. 2005..PERV-specific immune response of recipients was analysed by Western blot. No evidence of PERV infection or PERV-specific immune response was detected...