Research Topics
Species | Peter NeubauerSummaryCountry: Germany Publications
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Publications
Scale-down simulators for metabolic analysis of large-scale bioprocessesPeter Neubauer
Technische Universitat Berlin, Institute of Biotechnology, Department of Bioprocess Engineering, Ackerstr 71 76, D 13355 Berlin, Germany
Curr Opin Biotechnol 21:114-21. 2010..These simulators will pave the way for screening robust strains and process conditions...- Complete genome sequence of the lytic Pseudomonas fluorescens phage ?IBB-PF7ASanna Sillankorva
IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar 4710 057, Braga, Portugal
Virol J 8:142. 2011..This work describes the complete genome sequence of this phage... - Heterologous production of active ribonuclease inhibitor in Escherichia coli by redox state control and chaperonin coexpressionJuozas Siurkus
Thermo Fisher Scientific, V Graiciuno 8, LT 02241 Vilnius, Lithuania
Microb Cell Fact 10:65. 2011..After optimisation of the production conditions in the shake flask scale the process was scaled up to high cell densities by applying a glucose limited fed-batch procedure... - Enhanced growth and recombinant protein production of Escherichia coli by a perfluorinated oxygen carrier in miniaturized fed-batch culturesMaciej Pilarek
Department of Biotechnology and Bioprocess Engineering, Faculty of Chemical and Process Engineering, Warsaw University of Technology, Poland
Microb Cell Fact 10:50. 2011.... - Reducing conditions are the key for efficient production of active ribonuclease inhibitor in Escherichia coliJuozas Siurkus
Thermo Fisher Scientific V A Graiciuno, Vilnius, Lithuania
Microb Cell Fact 10:31. 2011..The members of this group are difficult to produce in E. coli and other recombinant hosts due to their high aggregation tendency... - Fed-batch process for the psychrotolerant marine bacterium Pseudoalteromonas haloplanktisBoris Wilmes
Institute of Marine Biotechnology, W Rathenau Str 49, D 17489 Greifswald, Germany
Microb Cell Fact 9:72. 2010..Here we present the first fed-batch cultivation strategy for this auspicious alternative expression host... - Enzyme controlled glucose auto-delivery for high cell density cultivations in microplates and shake flasksJohanna Panula-Perälä
Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering and Biocenter Oulu, University of Oulu, P, O, Box 4300, FI 90014 Oulu, Finland
Microb Cell Fact 7:31. 2008..abstract:.. - High-yield production of biologically active recombinant protein in shake flask culture by combination of enzyme-based glucose delivery and increased oxygen transferKaisa Ukkonen
BioSilta Oy, FI 90014 Oulu, Finland
Microb Cell Fact 10:107. 2011..Four-fold increase in oxygen transfer by the Ultra Yield Flask contributed to higher cell density with EnBase but not with the other tested media, and consequently the product yield per ml of EnBase culture was further improved... - Structure-based protein engineering efforts with a monomeric TIM variant: the importance of a single point mutation for generating an active site with suitable binding propertiesMarkus Alahuhta
Biocenter Oulu, University of Oulu, Oulu, Finland
Protein Eng Des Sel 21:257-66. 2008..The binding of this citrate molecule is stabilized by hydrogen bonding interactions with the new binding groove... - A novel fed-batch based cultivation method provides high cell-density and improves yield of soluble recombinant proteins in shaken culturesMirja Krause
BioSilta Oy, c o Department of Biochemistry, University of Oulu, Finland
Microb Cell Fact 9:11. 2010..In this article we describe the impact of a novel enzymatically controlled fed-batch cultivation technology on recombinant protein production in Escherichia coli in simple shaken cultures... - Fermentation process for tetrameric human collagen prolyl 4-hydroxylase in Escherichia coli: improvement by gene optimisation of the PDI/beta subunit and repeated addition of the inducer anhydrotetracyclineAntje Neubauer
Collagen Research Unit, Biocenter Oulu and Department of Medical Biochemistry and Molecular Biology, P O Box 5000, University of Oulu, FIN 90014 Oulu, Finland
J Biotechnol 128:308-21. 2007..The optimisation approach resulted in a fermentation yield of 143 mg L(-1) of active C-P4H, corresponding to approximately 7.5% of the total soluble cell protein... - Structural studies show that the A178L mutation in the C-terminal hinge of the catalytic loop-6 of triosephosphate isomerase (TIM) induces a closed-like conformation in dimeric and monomeric TIMMarkus Alahuhta
Biocenter Oulu and Department of Biochemistry, University of Oulu, PO Box 3000, FIN 90014 University of Oulu, Finland
Acta Crystallogr D Biol Crystallogr 64:178-88. 2008..In the liganded structures of A178L and ml1A178L the structural differences induced by the mutation are minimal. Structural characterization of the ml1A178L variant highlights its structural plasticity... - Improved production of human type II procollagen in the yeast Pichia pastoris in shake flasks by a wireless-controlled fed-batch systemMaria Ruottinen
Bioprocess Engineering Laboratory, Dept, of Process and Environmental Engineering, University of Oulu, P O Box 4300, FIN 90014 University of Oulu, Finland
BMC Biotechnol 8:33. 2008..The development of feeding protocols and the study of AOX1-controlled recombinant protein production have been largely made in shake flasks, although shake flasks have very limited possibilities for measurement and control... - Quality control of inclusion bodies in Escherichia coliBritta Jürgen
Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering and Biocenter Oulu, University of Oulu, FIN 90014 Oulu, Finland
Microb Cell Fact 9:41. 2010..IBs mostly contain only minor impurities and are relatively homogenous... - A two-compartment bioreactor system made of commercial parts for bioprocess scale-down studies: impact of oscillations on Bacillus subtilis fed-batch cultivationsStefan Junne
Chair of Bioprocess Engineering, Department of Biotechnology, Technische Universitat Berlin, Berlin, Germany
Biotechnol J 6:1009-17. 2011..As a result, the reduced carbon flux entering the tricarboxylic acid cycle is not sufficient to support amino acid synthesis following the oxaloacetic acid branch point... - Modelling of translation of human protein disulfide isomerase in Escherichia coli-A case study of gene optimisationOlli Niemitalo
Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering, University of Oulu, Oulu, Finland
J Biotechnol 120:11-24. 2005..coli translational ribosome traffic based upon the stochastic Gillespie algorithm. The study indicates the applicability of such models in optimisation of recombinant protein sequences... - High cell density cultivation and recombinant protein production with Escherichia coli in a rocking-motion-type bioreactorJulia Glazyrina
Laboratory of Bioprocess Engineering, Department of Biotechnology, Technische Universitat Berlin, Ackerstrasse 71 76, D 13355 Berlin, Germany
Microb Cell Fact 9:42. 2010..Here, we investigate the possibilities which both strategies offer in view of high cell density cultivation of E. coli and recombinant protein production... - Novel approach of high cell density recombinant bioprocess development: optimisation and scale-up from microliter to pilot scales while maintaining the fed-batch cultivation mode of E. coli culturesJuozas Siurkus
Laboratory of Bioprocess Engineering, Department of Biotechnology, Technische Universitat Berlin, Ackerstr 71 76, D 13355 Berlin, Germany
Microb Cell Fact 9:35. 2010..Performing the screening already under fed-batch conditions would limit the amount of work and guarantee that the selected favoured conditions also work in the production scale... - Two-compartment method for determination of the oxygen transfer rate with electrochemical sensors based on sulfite oxidationJulia Glazyrina
Chair of Bioprocess Engineering, Department of Biotechnology, Technische Universitat Berlin, Berlin, Germany
Biotechnol J 6:1003-8. 2011..Under typical shaking conditions, k(L) a values in the standard baffled flasks reached values up to 220 h(-1) , whereas the k(L) a values of the Ultra Yield flasks were significantly higher (up to 422 h(-1) )... - Sandwich ELISA for quantitative detection of human collagen prolyl 4-hydroxylaseEkaterina Osmekhina
Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering, P O Box 4300, FIN 90014 University of Oulu, Finland
Microb Cell Fact 9:48. 2010..Then the following holoenzyme detection is accomplished by a goat anti-rabbit IgG labeled with alkaline phosphatase which AP catalyzes the reaction of a substrate transformation with fluorescent signal generation... - RNA-based sandwich hybridisation method for detection of lactic acid bacteria in brewery samplesSanna Huhtamella
Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering, Biocenter Oulu, University of Oulu, P O Box 4300, FI 90014 Oulu, Finland
J Microbiol Methods 68:543-53. 2007.... - Glucose-limited high cell density cultivations from small to pilot plant scale using an enzyme-controlled glucose delivery systemJulia Glazyrina
Laboratory of Bioprocess Engineering, Department of Biotechnology, Technische Universitat Berlin, Ackerstraße 71 76, ACK 24, D 13355 Berlin, Germany
N Biotechnol 29:235-42. 2012..Because of the linear cell growth under glucose limitation it provides optimal and robust starting conditions for traditional external feed-based processes... - Modification of buffered peptone water for improved recovery of heat-injured Salmonella TyphimuriumSanna Taskila
Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering, University of Oulu, P O Box 4300, Oulu FI 90014, Finland
J Food Sci 76:M157-62. 2011..The presented results can be used for the development of more efficient enrichment cultivation media for faster detection of food borne?Salmonella... - Isolation and genotype-dependent, organ-specific expression analysis of a Rhodiola rosea cDNA encoding tyrosine decarboxylaseZsuzsanna György
Department of Process and Environmental Engineering, University of Oulu, 90014 Oulu, Finland
J Plant Physiol 166:1581-6. 2009..The difference in the expression intensity suggests a decisive role for this enzyme in the salidroside biosynthesis... - Norvaline is accumulated after a down-shift of oxygen in Escherichia coli W3110Jaakko Soini
Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering and Biocenter Oulu, University of Oulu, P, O, Box 4300, FI 90014 Oulu, Finland
Microb Cell Fact 7:30. 2008..abstract:.. - High-level production of human collagen prolyl 4-hydroxylase in Escherichia coliAntje Neubauer
Collagen Research Unit, Biocenter Oulu and Department of Medical Biochemistry and Molecular Biology, P. O. Box 5000, University of Oulu, FIN-90014 Oulu, Finland
Matrix Biol 24:59-68. 2005..The enzyme expressed in E. coli differed from those present in vivo and those produced in other hosts in that it lacked the N glycosylation of its alpha subunits, which may be advantageous in crystallization experiments... - Two-dimensional proteome reference map for the radiation-resistant bacterium Deinococcus geothermalisChristina Liedert
Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering and Biocenter Oulu, University of Oulu, Linnanmaa, Oulu, Finland
Proteomics 10:555-63. 2010..A total of 34 abundant proteins with unknown function may relate to the extreme stress tolerance of D. geothermalis... - Inclusion bodies: formation and utilisationBeatrix Fahnert
Biocenter Oulu, Department of Process and Environmental Engineering, Bioprocess Engineering Laboratory, University of Oulu, PO Box 4300, 90014 Oulu, Finland
Adv Biochem Eng Biotechnol 89:93-142. 2004..In addition, simple strategies have been developed to optimise the purification and renaturation of disulfide bond containing proteins making a fast transfer of such processes into the industrial production scale realistic... - High-temperature cultivation and 5' mRNA optimization are key factors for the efficient overexpression of thermostable Deinococcus geothermalis purine nucleoside phosphorylase in Escherichia coliKathleen Szeker
Chair of Bioprocess Engineering, Department of Biotechnology, Technische Universitat Berlin, Ackerstr 71 76, ACK24, D 13355 Berlin, Germany
J Biotechnol 156:268-74. 2010..We suggest that this method is especially suitable for improving the expression of proteins derived from thermophiles in E. coli... - Murine Wnt-1 with an internal c-myc tag recombinantly produced in Escherichia coli can induce intracellular signaling of the canonical Wnt pathway in eukaryotic cellsBeatrix Fahnert
Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering, University of Oulu, P O Box 4300, FIN 90014 Oulu, Finland
J Biol Chem 279:47520-7. 2004..Thus, this approach could make recombinant murine Wnt-1 available as a good starting point for other Wnts needed, for example, for maintaining and differentiating stem cells, organ restoration therapy, and tissue engineering... - Biotechnology - 12th European CongressPeter Neubauer
University of Oulu, Department of Process and Environmental Engineering, Bioprocess Engineering Laboratory, Finland
IDrugs 8:809-12. 2005 - 16S rRNA targeted sandwich hybridization method for direct quantification of mycobacteria in soilsTimo Nieminen
Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering and Biocenter Oulu, University of Oulu, Finland
J Microbiol Methods 67:44-55. 2006..The 16S rRNA sandwich hybridization method described in this paper offers a culture independent solution for tracking environmental reservoirs of viable and potentially infectious mycobacteria... - Octaketide-producing type III polyketide synthase from Hypericum perforatum is expressed in dark glands accumulating hypericinsKatja Karppinen
Department of Biology, University of Oulu, Finland
FEBS J 275:4329-42. 2008..Based on our results, HpPKS2 may have a role in the biosynthesis of hypericins in H. perforatum but some additional factors are possibly required for the production of emodin anthrone in vivo... - High cell density media for Escherichia coli are generally designed for aerobic cultivations - consequences for large-scale bioprocesses and shake flask culturesJaakko Soini
Bioprocess Engineering Laboratory, Dept, of Process and Environmental Engineering, University of Oulu, P, O, Box 4300, FIN 90014 Oulu, Finland
Microb Cell Fact 7:26. 2008..abstract:.. - Efficient lactic acid production from high salt containing dairy by-products by Lactobacillus salivarius ssp. salicinius with pre-treatment by proteolytic microorganismsAntti Vasala
Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering, University of Oulu, FIN 90014 Oulu, Finland
J Biotechnol 117:421-31. 2005..The results show that fast and complete conversion of lactose to lactic acid can be obtained in dairy by-products without expensive additives... - A role for bacteriophage T4 rI gene function in the control of phage development during pseudolysogeny and in slowly growing host cellsMarcin Los
Biocenter Oulu and Department of Process and Environmental Engineering, University of Oulu, Oulu, Finland
Res Microbiol 154:547-52. 2003..The rI gene, previously known to be indispensable for lysis inhibition, seems to play an important role in optimization of phage development in slowly growing cells as well as during establishment and maintenance of pseudolysogeny... - Functional role of the conserved active site proline of triosephosphate isomeraseMarco G Casteleijn
Department of Process and Environmental Engineering and Bioprocess Engineering Laboratory, University of Oulu, P.O. Box 3000, FIN-90014 Oulu, Finland
Biochemistry 45:15483-94. 2006..Apparently, the rotation of 90 degrees of the Gly211-Gly212 peptide plane of loop 7 plays a key role in this concerted movement...