Research Topics
| O FiehnSummaryAffiliation: Max Planck Institute of Molecular Plant Physiology Country: Germany Publications
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Detail Information
Publications
Identification of uncommon plant metabolites based on calculation of elemental compositions using gas chromatography and quadrupole mass spectrometryO Fiehn
Max Planck Institute of Molecular Plant Physiology, Potsdam, Germany
Anal Chem 72:3573-80. 2000..More than 15 uncommon plant metabolites were identified, some of which are novel in Arabidopsis, such as tartronate semialdehyde, citramalic acid, allothreonine, or glycolic amide...- Deciphering metabolic networksOliver Fiehn
Max Planck Institute of Molecular Plant Physiology, 14424 Potsdam Golm, Germany
Eur J Biochem 270:579-88. 2003..Methods to study such networks are reviewed with respect to data acquisition, network statistics, and biochemical interpretation... - Metabolite profiling for plant functional genomicsO Fiehn
Max Planck Institute of Molecular Plant Physiology, 14424 Potsdam, Germany
Nat Biotechnol 18:1157-61. 2000..These results demonstrate the use of metabolite profiling as a tool to significantly extend and enhance the power of existing functional genomics approaches... - Integrated studies on plant biology using multiparallel techniquesO Fiehn
Max Planck Institut fur Molekulare Pflanzenphysiologie, Am Muhlenberg 1, 14476 Golm, Germany
Curr Opin Biotechnol 12:82-6. 2001..Profiling strategies are putting this change into effect through the generation of large amounts of data, requiring that current bioinformatic approaches adapt and grow in order to make the most of these data... - Metabolomics--the link between genotypes and phenotypesOliver Fiehn
Max Planck Institute of Molecular Plant Physiology, Potsdam, Germany
Plant Mol Biol 48:155-71. 2002..Current approaches are examined, and potential applications are summarized with a special emphasis on data mining and mathematical modelling of metabolism... - Metabolic networks of Cucurbita maxima phloemOliver Fiehn
Max Planck Institute of Molecular Plant Physiology, D 14424, Potsdam Golm, Germany
Phytochemistry 62:875-86. 2003..Using metabolic co-regulation analysis, similarities and differences between the exudate profiles were more accurately characterized through network computation, specifically with respect to nitrogen metabolism... - Metabolic profiling allows comprehensive phenotyping of genetically or environmentally modified plant systemsU Roessner
, , 14476 Golm, Germany
Plant Cell 13:11-29. 2001..In summary, these data demonstrate the use of metabolic profiling in conjunction with data-mining tools as a technique for the comprehensive characterization of a plant genotype... - Systems rebalancing of metabolism in response to sulfur deprivation, as revealed by metabolome analysis of Arabidopsis plantsVictoria J Nikiforova
Max Planck Institute of Molecular Plant Physiology, Golm 14476, Germany
Plant Physiol 138:304-18. 2005..These responses may be assembled into a global scheme of metabolic regulation induced by sulfur nutritional stress, which optimizes resources for seed production... - Process for the integrated extraction, identification and quantification of metabolites, proteins and RNA to reveal their co-regulation in biochemical networksWolfram Weckwerth
Max Planck Institut fur Molekulare Pflanzenphysiologie, Golm, Germany
Proteomics 4:78-83. 2004.... - Identification of metabolic and biomass QTL in Arabidopsis thaliana in a parallel analysis of RIL and IL populationsJan Lisec
Max Planck Institute of Molecular Plant Physiology, Am Muehlenberg 1, 14476 Potsdam Golm, Germany
Plant J 53:960-72. 2008..5. This dataset thus provides a comprehensive basis for the detection of functionally relevant variation in known genes with metabolic function and for identification of genes with hitherto unknown roles in the control of metabolism... - Metabolite profiling of Chlamydomonas reinhardtii under nutrient deprivationChristian Bölling
Max Planck Institute of Molecular Plant Physiology, 14424 Potsdam, Germany
Plant Physiol 139:1995-2005. 2005.... - Can we discover novel pathways using metabolomic analysis?Wolfram Weckwerth
Max Planck Institute of Molecular Plant Physiology, Department Willmitzer, 14424 Potsdam, Germany
Curr Opin Biotechnol 13:156-60. 2002..The development of metabolomic methods and tools is progressing rapidly, but an understanding of the resulting data is limited owing to a fundamental lack of biochemical and physiological knowledge about network organization in plants... - Analysis of highly polar compounds of plant origin: combination of hydrophilic interaction chromatography and electrospray ion trap mass spectrometryVladimir V Tolstikov
Department of Lothar Willmitzer, Max Planck Institute of Molecular Plant Physiology, Potsdam, 14424, Germany
Anal Biochem 301:298-307. 2002..The detection limit for stachyose was 0.5 ng per injection (Amide 80). The concentration of stachyose in investigated phloem samples was in the range of 1-7 mM depending on the plant... - Visualizing plant metabolomic correlation networks using clique-metabolite matricesF Kose
Max Planck Institute of Molecular Plant Physiology, Department of Lothar Willmitzer, Postfach, 14424 Potsdam, Germany
Bioinformatics 17:1198-208. 2001..However, graph complexity rapidly increases with the number of edges and vertices. To gain structural information from metabolite correlation networks, improvements in clarity are needed... - Comparative quantification and identification of phosphoproteins using stable isotope labeling and liquid chromatography/mass spectrometryW Weckwerth
Max Planck Institute of Molecular Plant Physiology, 14424 Potsdam, Germany
Rapid Commun Mass Spectrom 14:1677-81. 2000..986). Additionally, the method allowed protein identification and determination of the phosphorylation sites via MS/MS fragmentation... - Linking protein fractionation with multidimensional monolithic reversed-phase peptide chromatography/mass spectrometry enhances protein identification from complex mixtures even in the presence of abundant proteinsStefanie Wienkoop
, 14424 Potsdam, Germany
Rapid Commun Mass Spectrom 18:643-50. 2004..The proposed strategy is useful to detect proteins otherwise not seen in conventional multidimensional chromatography/mass spectrometry approaches... - Differential metabolic networks unravel the effects of silent plant phenotypesWolfram Weckwerth
Department of Molecular Physiology, Max Planck Institute of Molecular Plant Physiology, 14424 Potsdam, Germany
Proc Natl Acad Sci U S A 101:7809-14. 2004..Metabolic alterations could be assigned to carbohydrate and amino acid metabolism even if no difference in average metabolite levels was found... - Metabolite fingerprinting: detecting biological features by independent component analysisM Scholz
Max Planck Institute of Molecular Plant Physiology, 14424 Potsdam, Germany
Bioinformatics 20:2447-54. 2004..The kurtosis measure is used to order the extracted components to our interest. Applied to our A. thaliana data, ICA detects three relevant factors, two biological and one technical, and clearly outperforms the PCA... - Robust detection and verification of linear relationships to generate metabolic networks using estimates of technical errorsFrank Kose
Universitaet Potsdam, Potsdam, Germany
BMC Bioinformatics 8:162. 2007..The entirety of all pair wise linear metabolic relationships then yields insights into the network of cellular regulation... - Statistical methods for comparing comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry results: metabolomic analysis of mouse tissue extractsRobert A Shellie
Max Planck Institute of Molecular Plant Physiology, Potsdam-Golm, Germany
J Chromatogr A 1086:83-90. 2005.... - Monolithic silica-based capillary reversed-phase liquid chromatography/electrospray mass spectrometry for plant metabolomicsVladimir V Tolstikov
Department Lothar Willmitzer, Max Planck Institute of Molecular Plant Physiology, 14424 Potsdam, Germany
Anal Chem 75:6737-40. 2003.... - The metabolic signature related to high plant growth rate in Arabidopsis thalianaRhonda C Meyer
Department of Genetics, Institute of Biochemistry and Biology, University of Potsdam, Karl Liebknecht Strasse 24 25, 14476 Potsdam, Germany
Proc Natl Acad Sci U S A 104:4759-64. 2007.... - Profiling at mRNA, protein, and metabolite levels reveals alterations in renal amino acid handling and glutathione metabolism in kidney tissue of Pept2-/- miceIsabelle M Frey
Molecular Nutrition Unit, Technical University of Munich, Freising, Germany
Physiol Genomics 28:301-10. 2007.... - Application of liquid chromatography-mass spectrometry analysis in metabolomics: reversed-phase monolithic capillary chromatography and hydrophilic chromatography coupled to electrospray ionization-mass spectrometryVladimir V Tolstikov
Genome Center, University of California Davis, USA
Methods Mol Biol 358:141-55. 2007..We have demonstrated that reversed-phase monolithic capillary chromatography and hydrophilic chromatography can be successfully applied for sufficient plant crude extracts separations and metabolomics studies... - Metabolomics Standards Workshop and the development of international standards for reporting metabolomics experimental resultsArthur L Castle
Metabolomics and Informatics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health NIH 6707 Democracy Blvd, Room 791, MSC 5460, Bethesda, Maryland 20892 5460, USA
Brief Bioinform 7:159-65. 2006..This report highlights specific aspects of what was presented and discussed at the 1st and 2nd August 2005 Metabolomics Standards Workshop... - Quality control for plant metabolomics: reporting MSI-compliant studiesOliver Fiehn
Davis Genome Center, University of California, Davis, CA 95616, USA
Plant J 53:691-704. 2008..Standardized data processing methods are proposed for consistent data storage and dissemination via databases... - Metabolite profiling in ArabidopsisOliver Fiehn
Max-Planck-Institute of Molecular Plant Physiology, Goln, Germany
Methods Mol Biol 323:439-47. 2006..It gives experimental details on the basic steps: harvest, homogenization, extraction, fractionation, concentration, derivatization, data acquisition, raw data processing and result data tranformation... - Plant metabolomics: the missing link in functional genomics strategiesRobert Hall
Plant Research International, BU Cell Cybernetics, P.O. Box 16, 6700 AA Wageningen, The Netherlands
Plant Cell 14:1437-40. 2002 - Application of metabolomics to plant genotype discrimination using statistics and machine learningJanet Taylor
Department of Computer Science, University of Wales, Aberystwyth, Penglais, UK
Bioinformatics 18:S241-8. 2002..These results are consistant with genotype differences in mitochondia and chloroplasts... - A prominent role for the CBF cold response pathway in configuring the low-temperature metabolome of ArabidopsisDaniel Cook
Michigan State University-Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, MI 48824, USA
Proc Natl Acad Sci U S A 101:15243-8. 2004..Taken together, the results indicate that the metabolome of Arabidopsis is extensively reconfigured in response to low temperature, and that the CBF cold response pathway has a prominent role in this process... - Potential of metabolomics as a functional genomics toolRaoul J Bino
Plant Physiology Department, Wageningen University, Arboretumlaan 4, 6703 BD Wageningen, The Netherlands
Trends Plant Sci 9:418-25. 2004 - Metabolomic database annotations via query of elemental compositions: mass accuracy is insufficient even at less than 1 ppmTobias Kind
University of California Davis, Genome Center, Davis, CA 95616, USA
BMC Bioinformatics 7:234. 2006..This report demonstrates the necessity of isotope abundance information by mathematical confirmation of the concept... - A proposed framework for the description of plant metabolomics experiments and their resultsHelen Jenkins
Department of Computer Science, University of Wales, Penglais, Aberystwyth, Ceredigion, Wales, UK
Nat Biotechnol 22:1601-6. 2004..armet.org/). We seek to advance discussion and community adoption of a standard for metabolomics, which would promote principled collection, storage and transmission of experiment data... - The metabolomics standards initiativeSusanna-Assunta Sansone
Nat Biotechnol 25:846-8. 2007 - Highly efficient monolithic silica capillary columns modified with poly(acrylic acid) for hydrophilic interaction chromatographyKanta Horie
Department of Biomolecular Engineering, Kyoto Institute of Technology, Matsugasaki, Kyoto, Japan
J Chromatogr A 1164:198-205. 2007.... - Promoting coherent minimum reporting guidelines for biological and biomedical investigations: the MIBBI projectChris F Taylor
European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SD, UK
Nat Biotechnol 26:889-96. 2008 - A comprehensive urinary metabolomic approach for identifying kidney cancerrTobias Kind
Genome Center, University of California, Davis, CA 95616, USA
Anal Biochem 363:185-95. 2007..Such investigation will likely lead to clinically applicable assays for earlier diagnosis of RCC, as well as other malignancies, and thereby improved patient prognosis... - Pathway analysis of kidney cancer using proteomics and metabolic profilingBertrand Perroud
Genome Center, University of California, Davis, CA, USA
Mol Cancer 5:64. 2006.... - Applications of metabolomics in agricultureRichard A Dixon
Plant Biology Division, Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA
J Agric Food Chem 54:8984-94. 2006..Selected presentations from an American Chemical Society symposium held in March 2005 have been assembled to highlight the emerging application of metabolomics in agriculture... - Mass spectrometry-based metabolic profiling reveals different metabolite patterns in invasive ovarian carcinomas and ovarian borderline tumorsCarsten Denkert
Institute of Pathology and Department of Gynecology and Obstetrics, Charite University Hospital, Berlin, Germany
Cancer Res 66:10795-804. 2006..We conclude that metabolomics is a promising high-throughput, automated approach in addition to functional genomics and proteomics for analyses of molecular changes in malignant tumors... - Comparison of rapid liquid chromatography-electrospray ionization-tandem mass spectrometry methods for determination of glycoalkaloids in transgenic field-grown potatoesBritta Zywicki
Max-Planck-Institute of Molecular Plant Physiology, 14424 Potsdam-Golm, Germany
Anal Biochem 336:178-86. 2005..We suggest complementing current unbiased metabolomic strategies by validating quantitative analytical methods for important target analytes such as the toxic glycoalkaloids in potato plants... - Metabolite profiling in blood plasmaOliver Fiehn
Genome Center, University of California Davis, USA
Methods Mol Biol 358:3-17. 2007..A major difference to profiling plant tissues is that no fractionation step is utilized, enabling the analysis of primary metabolites like sugars and amino acids concomitant with lipids such as sterols and free fatty acids... - Establishing reporting standards for metabolomic and metabonomic studies: a call for participationOliver Fiehn
UC Davis Genome Center, Davis, California 95616, USA
OMICS 10:158-63. 2006..These include biological study designs, chemical analysis, and data processing, as well as the ontologies that are necessary in this framework... - Natural genetic variation of freezing tolerance in ArabidopsisMatthew A Hannah
, D-14424 Potsdam, Germany
Plant Physiol 142:98-112. 2006.... - Hierarchical metabolomics demonstrates substantial compositional similarity between genetically modified and conventional potato cropsGareth S Catchpole
Max Planck Institute for Molecular Plant Physiology, D-14424 Golm, Germany
Proc Natl Acad Sci U S A 102:14458-62. 2005..We show that, apart from targeted changes, these GM potatoes in this study appear substantially equivalent to traditional cultivars... - The metabolic profiles of transgenic cucumber lines vary with different chromosomal locations of the transgeneNorikazu Tagashira
Faculty of Human Life Science, Hiroshima Jogakuin University, Japan
Cell Mol Biol Lett 10:697-710. 2005..The observed effects of transgene introduction are discussed in this paper... - Metabolite profiling of human colon carcinoma--deregulation of TCA cycle and amino acid turnoverCarsten Denkert
Institute of Pathology, Charite University Hospital, 10117 Berlin, Germany
Mol Cancer 7:72. 2008..However, in contrast to genomics and proteomics, comprehensive metabolomic investigations of alterations in malignant tumors have rarely been conducted...