Bernhard Michalke

Summary

Affiliation: GSF-National Research Center for Environment and Health
Country: Germany

Publications

  1. ncbi Selenium speciation by interfacing capillary electrophoresis with inductively coupled plasma-mass spectrometry
    B Michalke
    National Research Center for Environment and Health Institute of Ecological Chemistry, Neuherberg, Germany
    Electrophoresis 19:270-5. 1998
  2. ncbi Manganese species from human serum, cerebrospinal fluid analyzed by size exclusion chromatography-, capillary electrophoresis coupled to inductively coupled plasma mass spectrometry
    Bernhard Michalke
    GSF National Research Center of Environment and Health, Institute of Ecological Chemistry, 85764 Neuherberg, Germany
    J Trace Elem Med Biol 21:4-9. 2007
  3. ncbi Speciation and toxicological relevance of manganese in humans
    Bernhard Michalke
    GSF National Research Center for Environment and Health, Institute of Ecological Chemistry, 85764 Neuherberg, Germany
    J Environ Monit 9:650-6. 2007
  4. ncbi Manganese speciation in human cerebrospinal fluid using CZE coupled to inductively coupled plasma MS
    Bernhard Michalke
    GSF National Research Center for Environment and Health, Institute of Ecological Chemistry, Neuherberg, Germany
    Electrophoresis 28:1380-6. 2007
  5. ncbi Capillary electrophoresis-inductively coupled plasma-mass spectrometry: a report on technical principles and problem solutions, potential, and limitations of this technology as well as on examples of application
    Bernhard Michalke
    GSF National Research Center for Environment and Health, Institute of Ecological Chemistry, Neuherberg, Germany
    Electrophoresis 26:1584-97. 2005
  6. ncbi Selenium speciation in human serum of cystic fibrosis patients compared to serum from healthy persons
    Bernhard Michalke
    GSF National Research Center for Environment and Health, Institute of Ecological Chemistry, Ingolstadter Landstrasse 1, Neuherberg Oberschleissheim D 85764, Germany
    J Chromatogr A 1058:203-8. 2004
  7. ncbi Manganese speciation using capillary electrophoresis-ICP-mass spectrometry
    Bernhard Michalke
    GSF National Research Center for Environment and Health, Ingolstädter Landstreet 1, 85764 Neuherberg, Germany
    J Chromatogr A 1050:69-76. 2004
  8. ncbi Element speciation definitions, analytical methodology, and some examples
    Bernhard Michalke
    GSF National Research Center for Environment and Health, Ingolstaedter Landstr 1, 85764 Neuherberg, Germany
    Ecotoxicol Environ Saf 56:122-39. 2003
  9. ncbi Effect of different extraction procedures on the yield and pattern of Se-species in bacterial samples
    B Michalke
    GSF National Research Center for Environment and Health, Institute of Ecological Chemistry, Neuherberg, Germany
    Anal Bioanal Chem 372:444-7. 2002
  10. ncbi Capillary electrophoresis interfaced to inductively coupled plasma mass spectrometry for element selective detection in arsenic speciation
    B Michalke
    GSF National Research Center for Environment and Health, Institute for Ecological Chemistry, Neuherberg, Germany
    Electrophoresis 19:2220-5. 1998

Collaborators

Detail Information

Publications35

  1. ncbi Selenium speciation by interfacing capillary electrophoresis with inductively coupled plasma-mass spectrometry
    B Michalke
    National Research Center for Environment and Health Institute of Ecological Chemistry, Neuherberg, Germany
    Electrophoresis 19:270-5. 1998
    ..Detection limits were calculated as 10 or 20 microg Se/L for inorganic Se species and 35-50 microg Se/L for organic Se species...
  2. ncbi Manganese species from human serum, cerebrospinal fluid analyzed by size exclusion chromatography-, capillary electrophoresis coupled to inductively coupled plasma mass spectrometry
    Bernhard Michalke
    GSF National Research Center of Environment and Health, Institute of Ecological Chemistry, 85764 Neuherberg, Germany
    J Trace Elem Med Biol 21:4-9. 2007
    ..CSF was further analyzed by CZE-ICP-DRC-MS. Several Mn-species were monitored and mostly identified. The most abundant Mn-species was Mn-citrate at a concentration of around 0.7 microg Mn L(-1)...
  3. ncbi Speciation and toxicological relevance of manganese in humans
    Bernhard Michalke
    GSF National Research Center for Environment and Health, Institute of Ecological Chemistry, 85764 Neuherberg, Germany
    J Environ Monit 9:650-6. 2007
    ....
  4. ncbi Manganese speciation in human cerebrospinal fluid using CZE coupled to inductively coupled plasma MS
    Bernhard Michalke
    GSF National Research Center for Environment and Health, Institute of Ecological Chemistry, Neuherberg, Germany
    Electrophoresis 28:1380-6. 2007
    ..By far the most abundant Mn species was Mn-citrate showing a concentration of 0.7 +/- 0.13 microg Mn/L. Interestingly, several other Mn species can be related to the citric acid cycle...
  5. ncbi Capillary electrophoresis-inductively coupled plasma-mass spectrometry: a report on technical principles and problem solutions, potential, and limitations of this technology as well as on examples of application
    Bernhard Michalke
    GSF National Research Center for Environment and Health, Institute of Ecological Chemistry, Neuherberg, Germany
    Electrophoresis 26:1584-97. 2005
    ..Different solutions are reported on these problems and summarized here. Finally numerous applications are reported. Mostly, applications are performed on speciation of selenium, arsenic, metallothionein isoforms, mercury, or cobalt...
  6. ncbi Selenium speciation in human serum of cystic fibrosis patients compared to serum from healthy persons
    Bernhard Michalke
    GSF National Research Center for Environment and Health, Institute of Ecological Chemistry, Ingolstadter Landstrasse 1, Neuherberg Oberschleissheim D 85764, Germany
    J Chromatogr A 1058:203-8. 2004
    ..The lowered SeC values together with lowered cationic/neutral Se compounds (probably Se enzymes) point to a Se-depleted regulated pathway combined with a reduced capability of protective functions such as protection from peroxides...
  7. ncbi Manganese speciation using capillary electrophoresis-ICP-mass spectrometry
    Bernhard Michalke
    GSF National Research Center for Environment and Health, Ingolstädter Landstreet 1, 85764 Neuherberg, Germany
    J Chromatogr A 1050:69-76. 2004
    ..Several Mn species were found, such as arginase, Mn-transferrine, Mn-albumine and some more. The detection limit of the method was determined at 1.1 microg Mn/L independent on the species...
  8. ncbi Element speciation definitions, analytical methodology, and some examples
    Bernhard Michalke
    GSF National Research Center for Environment and Health, Ingolstaedter Landstr 1, 85764 Neuherberg, Germany
    Ecotoxicol Environ Saf 56:122-39. 2003
    ..Finally, several elements for which the issue of speciation is important are discussed, with examples from some recent publications on arsenic, selenium and iodine speciation...
  9. ncbi Effect of different extraction procedures on the yield and pattern of Se-species in bacterial samples
    B Michalke
    GSF National Research Center for Environment and Health, Institute of Ecological Chemistry, Neuherberg, Germany
    Anal Bioanal Chem 372:444-7. 2002
    ..The other approaches seemed not to extract many species. Hot water extraction was completely unsuitable, extracting only low amounts of a single, unknown species...
  10. ncbi Capillary electrophoresis interfaced to inductively coupled plasma mass spectrometry for element selective detection in arsenic speciation
    B Michalke
    GSF National Research Center for Environment and Health, Institute for Ecological Chemistry, Neuherberg, Germany
    Electrophoresis 19:2220-5. 1998
    ..Investigations were focused on possibly occurring interferences, e.g., ArCl+ interference at the monoisotope 75As. Finally, real samples from biomedical field (urine) and environmental field (sewage sludge) were analyzed...
  11. ncbi Speciation analysis of selected metals and determination of their total contents in paired serum and cerebrospinal fluid samples: An approach to investigate the permeability of the human blood-cerebrospinal fluid-barrier
    Volker Nischwitz
    Helmholtz Zentrum Munchen, German Research Center for Environmental Health, Institute of Ecological Chemistry, Ingolstaedter Landstr 1, 85764 Neuherberg, Germany
    Anal Chim Acta 627:258-69. 2008
    ..As part of the method validation the relative stability of complexes of albumin, transferrin and citrate with Mn, Fe, Cu and Zn was investigated...
  12. ncbi Electrospray ionisation with selected reaction monitoring for the determination of Mn-citrate, Fe-citrate, Cu-citrate and Zn-citrate
    Volker Nischwitz
    Helmholtz Zentrum Munchen, Institute of Ecological Chemistry, 85764 Neuherberg, Germany
    Rapid Commun Mass Spectrom 23:2338-46. 2009
    ....
  13. ncbi Perspective on element speciation
    Bernhard Michalke
    Helmholtz Zentrum Munchen German Research Center for Environmental Health, Institute of Ecological Chemistry, 85764 Neuherberg, Germany
    J Environ Monit 11:1754-6. 2009
    ..However, organic and inorganic matrix compounds at much higher concentration levels in a sample than trace elements make the determination of trace elements often rather difficult...
  14. ncbi A method for low volume and low Se concentration samples and application to paired cerebrospinal fluid and serum samples
    Bernhard Michalke
    Helmholtz Center Munich German Research Center for Environmental Health, Ingolstadter Landstr 1, 85764 Neuherberg, Germany
    J Trace Elem Med Biol 23:243-50. 2009
    ..When relating the paired Se concentrations of CSF samples to respective serum samples it turned out that Se-CSF (behind blood brain barrier (BBB)) is independent on Se-serum concentration (before BBB)...
  15. ncbi Measurements of daily urinary uranium excretion in German peacekeeping personnel and residents of the Kosovo region to assess potential intakes of depleted uranium (DU)
    U Oeh
    GSF National Research Center for Environment and Health, Institute of Radiation Protection, 85764 Neuherberg, Germany
    Sci Total Environ 381:77-87. 2007
    ..It is concluded that both peacekeeping personnel and residents serving or living in the Balkans, respectively, were not exposed to significant amounts of DU...
  16. ncbi Observation of changes in urinary excretion of thorium in humans following ingestion of a therapeutic soil
    V Höllriegl
    Institute of Radiation Protection, GSF National Research Center for Environment and Health, Ingolstadter Landstrasse 1, D 85764 Neuherberg, Germany
    J Environ Radioact 95:149-60. 2007
    ....
  17. ncbi Distribution pattern of inhaled ultrafine gold particles in the rat lung
    S Takenaka
    GSF Institute for Inhalation Biology, Neuherberg Munich, Germany
    Inhal Toxicol 18:733-40. 2006
    ..To a low degree, systemic particle translocation took place...
  18. ncbi Platinum speciation in clinical and environmental samples: scrutiny of data obtained by using electrophoresis techniques (flatbed and capillary)
    S Lustig
    University of Ghent, Institute for Nuclear Sciences, Laboratory for Analytical Chemistry, Gent, Belgium
    Electrophoresis 20:1627-33. 1999
    ..Previous literature data, in which electrophoresis was used for platinum speciation without mentioning the platinum recoveries, becomes questionable...
  19. ncbi Iodine species and the endocrine system: thyroid hormone levels in adult Danio rerio and developing Xenopus laevis
    R Simon
    GSF Institute of Ecological Chemistry, Neuherberg, Germany
    Anal Bioanal Chem 372:481-5. 2002
    ..The data demonstrate that the LC-ICP-MS method was successful at measuring I-, MIT, DIT, T4, T3, and rT3 in these two species. Furthermore, the method also detected five additional iodinated compounds which are currently unidentified...
  20. ncbi Study of the copper distribution in contaminated soils of hop fields by single and sequential extraction procedures
    O Schramel
    GSF National Research Centre for Environment and Health, Institute for Ecological Chemistry, Neuherberg, Germary
    Sci Total Environ 263:11-22. 2000
    ..After comparing the results of the different extraction procedures the environmental behaviour of copper and its risks for plants, micro-organisms and groundwater is discussed in brief...
  21. ncbi Speciation of Pt(II) and Pt(IV) in spiked extracts from road dust using on-line liquid chromatography-inductively coupled plasma mass spectrometry
    V Nischwitz
    GSF National Research Centre for Environment and Health, Institute of Ecological Chemistry, Ingolstadter Landstr 1, 85764 Neuherberg, Germany
    J Chromatogr A 1016:223-34. 2003
    ..Additionally, the elution pattern of further elements in the road dust extracts was monitored and total element contents were determined in unspiked road dust extracts...
  22. ncbi Identification and quantification of metallothionein isoforms and superoxide dismutase in spiked liver extracts using HPLC-ESI-MS offline coupling and HPLC-ICP-MS online coupling
    V Nischwitz
    GSF National Research Center for Environment and Health, Institute for Ecological Chemistry, Ingolstadter Landstrasse 1, 85764 Neuherberg, Germany
    Anal Bioanal Chem 375:145-56. 2003
    ..An alternative quantification is proposed for the extension towards other lesser or even unknown trace element species, especially the native porcine MT and SOD...
  23. ncbi JEM spotlight: metal speciation related to neurotoxicity in humans
    Bernhard Michalke
    Helmholtz Zentrum Munchen, Institute of Ecological Chemistry, 85764, Neuherberg, Germany
    J Environ Monit 11:939-54. 2009
    ..Current results indicate that knowledge on trace element speciation can contribute to a better understanding of the transport of metals across the neural barriers and potentially of their role in diseased human brains...
  24. ncbi Daily urinary excretion of uranium in members of the public of Southwest Nigeria
    Vera Höllriegl
    Helmholtz Center München, Research Unit Medical Radiation Physics and Diagnostics, Ingolstadter Landstrasse 1, 85764 Neuherberg, Germany
    Sci Total Environ 412:344-50. 2011
    ..The data can be considered as baseline values of urinary uranium in unexposed subjects in Nigeria...
  25. ncbi Long-term monitoring of arsenic and selenium species in contaminated groundwaters by HPLC and HG-AAS
    M Raessler
    GSF National Research Centre for Environment and Health, Institute of Eco logical Chemistry, Neuherberg, Germany
    Sci Total Environ 258:171-81. 2000
    ..When applied to groundwater wells from Kelheim (Germany) they revealed that arsenic (V) and selenium (VI) were the dominating species. The presence of arsenic (III) and selenium (IV) was assumed to be supported by organic matter...
  26. ncbi Review on metal speciation analysis in cerebrospinal fluid-current methods and results: a review
    Bernhard Michalke
    Helmholtz Zentrum Munchen, Institute of Ecological Chemistry, 85764 Neuherberg, Germany
    Anal Chim Acta 682:23-36. 2010
    ..Data on total concentrations of numerous elements in CSF as well as speciation information of elements such as Al, As, Ca, Cd, Cu, Fe, Mg, Mn, Hg, Pb, Se and Zn in CSF are summarized...
  27. ncbi Application of capillary zone electrophoresis-inductively coupled plasma mass spectrometry and capillary isoelectric focusing-inductively coupled plasma mass spectrometry for selenium speciation
    B Michalke
    GSF-National Research Centre for Environment and Health, Neuherberg, Germany
    J Chromatogr A 807:71-80. 1998
    ..Detection limits were around 10-30 micrograms Se l-1. The method was applied to standard mixtures and body fluids like human milk and serum...
  28. ncbi Genome-wide analysis of caesium and strontium accumulation in Saccharomyces cerevisiae
    Sabine Heuck
    Helmholtz Zentrum Munchen, Institut fur Strahlenschutz, Neuherberg, Germany
    Yeast 27:817-35. 2010
    ..Single candidates (e.g. KHA1) are presented which might be specifically responsible for Cs(+) homeostasis...
  29. ncbi Optimisation of extraction procedures for metallothionein-isoforms and superoxide dismutase from liver samples using spiking experiments
    Volker Nischwitz
    GSF-National Research Centre for Environment and Health, Institute of Ecological Chemistry, , 85764 Neuherberg, Germany
    Analyst 128:109-15. 2003
    ..Additionally the buffer concentration and the extractant-to-liver ratio were varied for optimisation. The metal balance of an extraction showed recoveries of 81% for Cd, 94% for Cu and 87% for Zn...
  30. ncbi Measurement of cerium in human breast milk and blood samples
    Vera Höllriegl
    Institute of Radiation Protection, Helmholtz Zentrum Munchen, German Research Center for Environmental Health, Neuherberg, Germany
    J Trace Elem Med Biol 24:193-9. 2010
    ..Possibly, the cerium content in plasma/serum could be an indicator for environmental cerium, which is not valid for breast milk...
  31. ncbi Antimony speciation in environmental samples by interfacing capillary electrophoresis on-line to an inductively coupled plasma mass spectrometer
    B Michalke
    GSF National Research Center for Environment and Health, Institute for Ecological Chemistry, Neuherberg, Germany
    J Chromatogr A 834:341-8. 1999
    ..1 microgram/1-0.7 microgram/1, depending on species, were achieved. When analysing liquid phases from fouling and sewage sludge up to eight antimony species were detected. Sb (V) as well as methylated Sb species were found...
  32. ncbi Iodine speciation in human serum by reversed-phase liquid chromatography-ICP-mass spectrometry
    B Michalke
    GSF National Research Center for Environment and Health, Institute for Ecological Chemistry, Neuherberg, Germany
    Biol Trace Elem Res 78:81-91. 2000
    ....
  33. ncbi Method developments for iodine speciation by reversed-phase liquid chromatography-ICP-mass spectrometry
    B Michalke
    GSF National Research Center for Environment and Health, Institute for Ecological Chemistry, Neuherberg, Germany
    Biol Trace Elem Res 78:67-79. 2000
    ..Detection limits were determined between 0.08 and 1.5 microg/L (iodine related), dependent on species. Appplications on a urine sample were performed, showing iodide as the predominant iodine species...
  34. ncbi Effect of selenium on thioredoxin reductase activity in Txnrd1 or Txnrd2 hemizygous mice
    Claudia Kiermayer
    Department of Comparative Medicine, GSF Research Centre for Environment and Health, D 85764 Neuherberg, Germany
    Biol Chem 388:1091-7. 2007
    ..The only exception was Txnrd2 activity in kidney, heart and muscle, where we found an additive effect...
  35. ncbi Selenium status alters tumour differentiation but not incidence or latency of pancreatic adenocarcinomas in Ela-TGF-alpha p53+/ mice
    Michaela Aichler
    Department of Comparative Medicine, GSF National Research Centre for Environment and Health, Germany
    Carcinogenesis 28:2002-7. 2007
    ..These data point at a new role of the trace element selenium in carcinogenesis...