Research Topics
Genomes and Genes
| Volker GerkeSummaryCountry: Germany Publications
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Publications
Annexin A8 displays unique phospholipid and F-actin binding propertiesVerena Goebeler
Institute for Medical Biochemistry, Centre for Molecular Biology of Inflammation, University of Munster, Von Esmarch Str 56, D 48149 Munster, Germany
FEBS Lett 580:2430-4. 2006..These properties suggest a role of annexin A8 in the organization of certain actin-associated membrane domains...- Annexins: linking Ca2+ signalling to membrane dynamicsVolker Gerke
Institute of Medical Biochemistry, Centre for Molecular Biology of Inflammation, University of Munster, Germany
Nat Rev Mol Cell Biol 6:449-61. 2005..These and related properties enable annexins to participate in several otherwise unrelated events that range from membrane dynamics to cell differentiation and migration... - Annexin A8 regulates late endosome organization and functionVerena Goebeler
Institute of Medical Biochemistry, Centre for Molecular Biology of Inflammation, University of Muenster, 48149 Muenster, Germany
Mol Biol Cell 19:5267-78. 2008.... - Characterization of the Ca2+ -regulated ezrin-S100P interaction and its role in tumor cell migrationJudith Austermann
Institute of Medical Biochemistry, Center for Molecular Biology of Inflammation, University of Muenster, 48149 Muenster, Germany
J Biol Chem 283:29331-40. 2008..Thus, a prometastatic role of ezrin and S100P that had been proposed based on their overexpression in highly metastatic cancers is probably due to a direct interaction between the two proteins and the S100P-mediated activation of ezrin... - S100A2 induces metastasis in non-small cell lung cancerEtmar Bulk
Department of Medicine, Hematology and Oncology, University of Munster, Munster, Germany
Clin Cancer Res 15:22-9. 2009..In this study, we analyzed the prognostic role of mRNA levels of all S100 proteins in early stage non-small cell lung cancer (NSCLC) patients as well as the pathogenetic of S100A2 in the development of metastasis in NSCLC... - S100P is a novel interaction partner and regulator of IQGAP1Annika Heil
Institute of Medical Biochemistry, Centre for Molecular Biology of Inflammation, University of Muenster, Von Esmarch Strasse 56, D 48149 Muenster, Germany
J Biol Chem 286:7227-38. 2011..Thus, S100P is a novel Ca(2+)-dependent regulator of IQGAP1 that can down-regulate the function of IQGAP1 as a signaling intermediate by direct interaction... - Generation and characterization of a novel, permanently active S100P mutantJudith Austermann
Center for Molecular Biology of Inflammation, Institute of Medical Biochemistry, Von Esmarch Str 56, 48149 Munster, Germany
Biochim Biophys Acta 1793:1078-85. 2009..Thus, S100P pa is a novel type of S100 protein mutant locked in a permanently active state that shows an unregulated complex formation with its cellular target ezrin... - Specific association of annexin 1 with plasma membrane-resident and internalized EGF receptors mediated through the protein core domainSusanne Radke
Institute for Medical Biochemistry, Centre for Molecular Biology of Inflammation, ZMBE, IZKF Münster, University of Munster, Von Esmarch Str 56, 48149 Munster, Germany
FEBS Lett 578:95-8. 2004..We found that this interaction was independent of a functional phosphorylation site in the annexin 1 N-terminal domain but mediated through the Ca2+ binding core domain... - Annexin A5 interacts with polycystin-1 and interferes with the polycystin-1 stimulated recruitment of E-cadherin into adherens junctionsArseni Markoff
Institute of Medical Biochemistry, Westfalian Wilhelms University of Muenster, Von Esmarch Str 56, 48149 Muenster, Germany
J Mol Biol 369:954-66. 2007..This polycystin-1 stimulated recruitment is significantly delayed by extracellular annexin A5... - Expression and functions of annexins in the kidneyArseni Markoff
Institute of Medical Biochemistry, Centre for Molecular Biology of Inflammation, University of Muenster, 48149 Muenster, Germany
Am J Physiol Renal Physiol 289:F949-56. 2005..Last but not least, some annexins seem to work as extracellular autocrine modulators of receptor function under different physiological conditions... - Annexin A1 released from apoptotic cells acts through formyl peptide receptors to dampen inflammatory monocyte activation via JAK/STAT/SOCS signallingDanute Pupjalis
Centre for Molecular Biology of Inflammation, and Interdisciplinary Clinical Research Centre, Institute of Medical Biochemistry, University of Muenster, Muenster, Germany
EMBO Mol Med 3:102-14. 2011..This identifies annexin A1 as part of the anti-inflammatory pattern of apoptotic cells and links the activation of FPRs to established signalling pathways triggering anti-inflammatory responses... - S100A10/p11: family, friends and functionsUrsula Rescher
Institute of Medical Biochemistry, Centre for Molecular Biology of Inflammation, University of Muenster, Von Esmarch Str 56, 48149 Muenster, Germany
Pflugers Arch 455:575-82. 2008..Based on these data, we propose that S100A10 functions as a linker tethering certain transmembrane proteins to annexin A2 thereby assisting their traffic to the plasma membrane and/or their firm anchorage at certain membrane sites... - VAMP3 is associated with endothelial weibel-palade bodies and participates in their Ca(2+)-dependent exocytosisInés Rojo Pulido
Institute of Medical Biochemistry, Centre for Molecular Biology of Inflammation, University of Munster, D 48149 Munster, Germany
Biochim Biophys Acta 1813:1038-44. 2011..This article is part of a Special Issue entitled: 11th European Symposium on Calcium... - Phospholipase D1 is specifically required for regulated secretion of von Willebrand factor from endothelial cellsJennifer Disse
Institute of Medical Biochemistry, Centre for Molecular Biology of Inflammation, University of Munster, Munster, Germany
Blood 113:973-80. 2009..Thus, different mechanisms appear to control the agonist-induced secretion of VWF and tPA, with only the former requiring PLD1... - Tyrosine phosphorylation of annexin A2 regulates Rho-mediated actin rearrangement and cell adhesionUrsula Rescher
Institute of Medical Biochemistry, Centre for Molecular Biology of Inflammation, and Interdisciplinary Clinical Research Centre, University of Muenster, 48149 Muenster, Germany
J Cell Sci 121:2177-85. 2008..Thus, a tyrosine phosphorylation switch in annexin A2 is an important event in triggering Rho/ROCK-dependent and actin-mediated changes in cell morphology associated with the control of cell adhesion... - Myosin Va acts in concert with Rab27a and MyRIP to regulate acute von-Willebrand factor release from endothelial cellsInés Rojo Pulido
Institute of Medical Biochemistry, Centre for Molecular Biology of Inflammation, University of Munster, Munster, Germany
Traffic 12:1371-82. 2011..These results indicate that a Rab27a/MyRIP/myosin Va complex is involved in linking WPB to the peripheral actin cytoskeleton of endothelial cells to allow full maturation and prevent premature secretion of vWF... - Adjuvant therapy with small hairpin RNA interference prevents non-small cell lung cancer metastasis development in miceEtmar Bulk
Departments of Medicine, Hematology and Oncology, University of Munster, Munster, Germany
Cancer Res 68:1896-904. 2008..05). These findings suggest that RNA interference-based therapy approaches can be highly effective in the adjuvant setting... - Agonists of proteinase-activated receptor-2 affect transendothelial migration and apoptosis of human neutrophilsVictoria M Shpacovitch
Department of Dermatology, IZKF Münster, and Boltzmann Institute for Immunobiology of the Skin, University of Munster, Germany
Exp Dermatol 16:799-806. 2007.... - Transcriptional profiling of human monocytes reveals complex changes in the expression pattern of inflammation-related genes in response to the annexin A1-derived peptide Ac1-25Carsten Lange
Institute of Medical Biochemistry, Centre for Molecular Biology of Inflammation, and Interdisciplinary Clinical Research Centre, University of Muenster, Muenster, Germany
J Leukoc Biol 82:1592-604. 2007..This identifies the annexin A1 N-terminal peptide as a stimulus, eliciting not only short-term, post-translational effects in human monocytes but also transcriptional changes, defining a more anti-inflammatory profile... - Rab3D and annexin A2 play a role in regulated secretion of vWF, but not tPA, from endothelial cellsMarkus Knop
Institute of Medical Biochemistry, Centre for Molecular Biology of Inflammation, University of Munster, Munster, Germany
EMBO J 23:2982-92. 2004..This indicates that distinct mechanisms underlie the acute secretion of vWF and tPA, enabling endothelial cells to fine-regulate the release of thrombogenic and fibrinolytic factors... - Regulation of epithelial and endothelial junctions by PAR proteinsKlaus Ebnet
Institute of Medical Biochemistry, ZMBE, University of Muenster, D 48149 Muenster, Germany
Front Biosci 13:6520-36. 2008..Here we discuss the mechanisms by which PAR proteins regulate the formation of cell junctions with a special emphasis on vertebrate epithelial cells... - Annexins--unique membrane binding proteins with diverse functionsUrsula Rescher
Institute of Medical Biochemistry, Center for Molecular Biology of Inflammation, University of Munster, Von Esmarch Strasse 56, Munster 48149, Germany
J Cell Sci 117:2631-9. 2004..Among other things, this is evident in annexin A1- and A2-knockout mice, which show impaired regulation of neutrophil extravasation and defects in plasmin generation, respectively... - Wnt signaling regulates transendothelial migration of monocytesLara Tickenbrock
Department of Medicine, Hematology and Oncology, University of Munster, Domagkstr 3, 48129 Munster, Germany
J Leukoc Biol 79:1306-13. 2006..Our study implicates for the first time a role of canonical Wnt signaling in inflammatory processes in monocytes... - Annexins: from structure to functionVolker Gerke
Institute for Medical Biochemistry, Center for Molecular Biology of Inflammation, University of Munster, Munster, Germany
Physiol Rev 82:331-71. 2002..Finally, deregulations in annexin expression and activity have been correlated with human diseases, e.g., in acute promyelocytic leukemia and the antiphospholipid antibody syndrome, and the term annexinopathies has been coined... - TRPV5 is internalized via clathrin-dependent endocytosis to enter a Ca2+-controlled recycling pathwayStan F J van de Graaf
Institute of Medical Biochemistry, Center for Molecular Biology of Inflammation, University of Munster, Von Esmarch Strasse 56, Munster 48149, Germany
J Biol Chem 283:4077-86. 2008..Furthermore, the endocytic recycling kinetics is decreased upon treatment with Ca(2+) chelator BAPTA-AM, indicating that the channel's trafficking pathways are dynamically controlled by Ca(2+)... - The C-terminal cytoplasmic tail of claudins 1 and 5 but not its PDZ-binding motif is required for apical localization at epithelial and endothelial tight junctionsClaas Rüffer
Institute of Medical Biochcmistry, Center for Molecular Biology of Inflammation, University of Munster, Munster, Germany
Eur J Cell Biol 83:135-44. 2004..This indicates that crucial determinants for stable TJ incorporation of claudins reside in a cytoplasmic C-terminal sequence which up to now has not been implicated in specific protein-protein interactions... - Myeloid-related proteins 8 and 14 induce a specific inflammatory response in human microvascular endothelial cellsDorothee Viemann
Institute of Experimental Dermatology, Integrated Functional Genomics, and the Interdisciplinary Clinical Research Center, University Hospital Muenster, University of Muenster, Röntgenstrasse 21, 48149 Muenster, Germany
Blood 105:2955-62. 2005..MRP8/MRP14 may represent novel targets for anti-inflammatory strategies... - Structural and functional characterisation of the mouse annexin A9 promoterMarcin Chlystun
Centre for Molecular Biology of Inflammation, Institute for Medical Biochemistry, University of Muenster Von Esmarch Str 56, D 48149 Muenster, Germany
Biochim Biophys Acta 1742:141-9. 2004..This suggests that the entire region containing the non-protein coding exon I and the adjacent intron I is involved in the regulation of mouse annexin A9 transcription... - Atypical properties displayed by annexin A9, a novel member of the annexin family of Ca(2+) and lipid binding proteinsVerena Goebeler
Institute of Medical Biochemistry, Centre for Molecular Biology of Inflammation, University of Munster, Von Esmarch Str 56, D 48149 Munster, Germany
FEBS Lett 546:359-64. 2003..These results indicate that annexin A9 is a unique member of the annexin family whose intracellular activity is not subject to Ca(2+) regulation... - MRP8 and MRP14 control microtubule reorganization during transendothelial migration of phagocytesThomas Vogl
Institute of Experimental Dermatology, Department of Pediatrics, University of Munster, Röntgenstrasse 21, D 48149 Munster, Germany
Blood 104:4260-8. 2004..Thus, the complex of MRP8/MRP14 is the first characterized molecular target integrating MAPK- and calcium-dependent signals during migration of phagocytes... - Ca2+ -regulated secretion of tissue-type plasminogen activator and von Willebrand factor in human endothelial cellsMarkus Knop
Institute of Medical Biochemistry, Centre for Molecular Biology of Inflammation, University of Munster, Von Esmarch Str 56, Munster, Germany
Biochim Biophys Acta 1600:162-7. 2002..Our data thus point to differences in the Ca(2+)-triggered secretion of vWF and tPA which could allow a fine-tuning of their release thereby ensuring a balanced physiological action... - S100 family members and trypsinogens are predictors of distant metastasis and survival in early-stage non-small cell lung cancerSven Diederichs
Department of Medicine, Hematology Oncology, University of Munster, Munster, Germany
Cancer Res 64:5564-9. 2004..For the first time, this implicates a role of S100 proteins and trypsinogens in the metastatic process of early-stage NSCLC... - Endothelial Rho signaling is required for monocyte transendothelial migrationAnke Strey
Institute of Medical Biochemistry, ZMBE, , , Germany
FEBS Lett 517:261-6. 2002..Thus, efficient leukocyte extravasation requires Rho signaling not only within the migrating leukocytes but also within the endothelial lining of the vessel wall... - The formation of the cAMP/protein kinase A-dependent annexin 2-S100A10 complex with cystic fibrosis conductance regulator protein (CFTR) regulates CFTR channel functionLee A Borthwick
Academic Unit of Child Health, The University of Sheffield, Sheffield Children s Hospital, Sheffield, S10 2TH, United Kingdom
Mol Biol Cell 18:3388-97. 2007..Furthermore, peptide Ac1-14 inhibited acetylcholine-induced short-circuit current measured across a sheet of intact intestinal biopsy. Our data suggests that the anx 2-S100A10/CFTR complex is important for CFTR function across epithelia... - The annexin 2-S100A10 complex and its association with TRPV6 is regulated by cAMP/PKA/CnA in airway and gut epitheliaLee A Borthwick
Academic Unit of Child Health, University of Sheffield, Stephenson Wing, Sheffield Children s Hospital, Sheffield, South Yorkshire S10 2TH, UK
Cell Calcium 44:147-57. 2008..In conclusion, our data demonstrates that cAMP/PKA/CnA signalling is important for anx 2-S100A10 complex formation and interaction with target molecules in both absorptive and secretory epithelia... - PTEN-mediated apical segregation of phosphoinositides controls epithelial morphogenesis through Cdc42Fernando Martin-Belmonte
Department of Anatomy, University of California, San Francisco, CA 94143, USA
Cell 128:383-97. 2007..Loss of function of PTEN, Anx2, Cdc42, or aPKC prevents normal development of the apical surface and lumen. We conclude that the mechanism of PTEN, PtdIns(4,5)P2, Anx2, Cdc42, and aPKC controls apical plasma membrane and lumen formation... - Cell-surface attachment of pedestal-forming enteropathogenic E. coli induces a clustering of raft components and a recruitment of annexin 2Nicole Zobiack
Institute for Medical Biochemistry, Center for Molecular Biology of Inflammation, Von-Esmarch-Str. 56, , Germany
J Cell Sci 115:91-8. 2002.... - Ca2+-dependent binding and activation of dormant ezrin by dimeric S100PMax Koltzscher
Institute for Medical Biochemistry, University of Muenster, Germany
Mol Biol Cell 14:2372-84. 2003..This identifies S100P as a novel activator of ezrin and indicates that activation of ezrin's cross-linking function can occur directly in response to Ca2+ transients... - The annexin 2/S100A10 complex controls the distribution of transferrin receptor-containing recycling endosomesNicole Zobiack
Institute for Medical Biochemistry, Center for Molecular Biology of Inflammation, D 48149 Munster, Germany
Mol Biol Cell 14:4896-908. 2003.... - Annexin II is required for apical transport in polarized epithelial cellsRalf Jacob
Department of Physiological Chemistry, School of Veterinary Medicine Hannover, Germany
J Biol Chem 279:3680-4. 2004..The annexin II-S100A10 complex plays therefore a crucial role in routing SAVs to the apical membrane of epithelial cells... - Annexin 2 is a phosphatidylinositol (4,5)-bisphosphate binding protein recruited to actin assembly sites at cellular membranesUrsula Rescher
Institute for Medical Biochemistry, Center for Molecular Biology of Inflammation, Von Esmarch Str 56, Munster 48149, Germany
J Cell Sci 117:3473-80. 2004..Thus, the direct interaction of annexin 2 with PtdIns(4,5)P(2) is a means of specifically recruiting the annexin 2-p11 complex to sites of membrane-associated actin assembly... - The molecular arrangement of membrane-bound annexin A2-S100A10 tetramer as revealed by scanning force microscopyManuela Menke
, Chemo- und Biosensorik, , 93040 Regensburg, Germany
Chembiochem 5:1003-6. 2004 - Annexin-actin interactionsMatthew J Hayes
Division of Cell Biology, Institute of Ophthalmology, University College London, 11-43 Bath Street, London EC1V 9EL, UK
Traffic 5:571-6. 2004..In this review we discuss the current state of knowledge on this topic, and consider how and where annexins may fit into the complex molecular machinery that regulates the actin cytoskeleton... - Annexin 2 promotes the formation of lipid microdomains required for calcium-regulated exocytosis of dense-core vesiclesSylvette Chasserot-Golaz
Centre National de la Recherche Scientifique, , IFR 37 des Neurosciences, 67084 Strasbourg Cedex, France
Mol Biol Cell 16:1108-19. 2005..We propose that annexin 2 acts as a calcium-dependent promoter of lipid microdomains required for structural and spatial organization of the exocytotic machinery... - Phosphatidylserine membrane domain clustering induced by annexin A2/S100A10 heterotetramerManuela Menke
, Chemo- und Biosensorik, , 93040 Regensburg, Germany
Biochemistry 44:15296-303. 2005..A depletion of these POPS domains was observed in the vicinity of annexin A2t protein domains. These results indicate that annexin A2t is a peripheral membrane-binding complex capable of inducing lipid segregation... - Requirement for annexin A1 in plasma membrane repairAnna K McNeil
Department of Cellular Biology and Anatomy, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, Georgia 30912, USA
J Biol Chem 281:35202-7. 2006..We propose that Ca2+ entering through a disruption locally induces annexin A1 binding to membranes, initiating emergency fusion events whenever and wherever required... - Functional expression of the epithelial Ca(2+) channels (TRPV5 and TRPV6) requires association of the S100A10-annexin 2 complexStan F J van de Graaf
Department of Cell Physiology, Nijmegen Center for Molecular Life Sciences, University Medical Center Nijmegen, PO Box 9101, NL 6500 HB Nijmegen, The Netherlands
EMBO J 22:1478-87. 2003..In conclusion, the S100A10-annexin 2 complex plays a crucial role in routing of TRPV5 and TRPV6 to plasma membrane... - Identification of an AHNAK binding motif specific for the Annexin2/S100A10 tetramerSandrine De Seranno
INSERM, EMI01 04, Grenoble, France
J Biol Chem 281:35030-8. 2006..Together, our data demonstrate that A2tBP1 is a specific A2t complex binding domain and may be a powerful tool to help elucidate A2t structure and cellular functions... - A splice form of polycystin-2, lacking exon 7, does not interact with polycystin-1Karl Hackmann
Institut fur Humangenetik, Universitatsklinikum Munster, Germany
Hum Mol Genet 14:3249-62. 2005..The predominant expression in brain indicates a function in this organ. The inability to interact with polycystin-1 expands further the PKD1-independent functions of polycystin-2 forms... - Cooperative adsorption of ezrin on PIP2-containing membranesAlexander Herrig
, , Tammannstrasse 2, , Germany
Biochemistry 45:13025-34. 2006..On the basis of the kinetic analysis, we were also able to reconstruct the adsorption isotherm... - Membrane composition affects the reversibility of annexin A2t binding to solid supported membranes: a QCM studyMichaela Ross
, , Wilhelm-Klemm-Strasse 2, , Germany
Biochemistry 42:3131-41. 2003..We propose a model discussing the results obtained in terms of two binding sites differing in their affinity due to lipid rearrangement... - Kinetics and thermodynamics of annexin A1 binding to solid-supported membranes: a QCM studyKatja Kastl
, Chemo- und Biosensorik, , 93040 Regensburg, Germany
Biochemistry 41:10087-94. 2002.... - Functional activation of the formyl peptide receptor by a new endogenous ligand in human lung A549 cellsUrsula Rescher
Center for Molecular Biology of Inflammation, Institute for Medical Biochemistry, , Germany
J Immunol 169:1500-4. 2002..Hence, activation of nonmyeloid FPR by its endogenous ligand annexin 1 could participate in the regulation of acute-phase responses, e.g., during inflammation and/or wound healing... - Proteolytic cleavage of annexin 1 by human leukocyte elastaseUrsula Rescher
Center for Molecular Biology of Inflammation, Institute for Medical Biochemistry, Von Esmarch Strasse 56, 48149 Munster, Germany
Biochim Biophys Acta 1763:1320-4. 2006..The identification of annexin 1 as a substrate for HLE supports the model in which annexin 1 participates in regulating leukocyte emigration into inflamed tissue through N-terminal peptides generated at inflammatory sites... - A common haplotype of the annexin A5 (ANXA5) gene promoter is associated with recurrent pregnancy lossNadia Bogdanova
Institut fur Humangenetik, Westfalian Wilhelms University of Münster and University Clinic Münster, Munster, Germany
Hum Mol Genet 16:573-8. 2007.... - Partially reversible adsorption of annexin A1 on POPC/POPS bilayers investigated by QCM measurements, SFM, and DMC simulationsKatja Kastl
, Chemo- und Biosensorik, , 93040 Regensburg, Germany
Chembiochem 7:106-15. 2006..Monte Carlo simulations suggest that the area of the POPS-enriched phase increases by a factor of 7 when the Ca2+ concentration is changed from 0.01 to 1 mM... - An annexin 1 N-terminal peptide activates leukocytes by triggering different members of the formyl peptide receptor familyStefanie Ernst
Institute for Medical Biochemistry, Center for Molecular Biology of Inflammation, Von Esmarch Strasse 56, D 48149 Munster, Germany
J Immunol 172:7669-76. 2004.... - Defective formation of PKA/CnA-dependent annexin 2-S100A10/CFTR complex in DeltaF508 cystic fibrosis cellsLee Anthony Borthwick
Academic Unit of Child Health, The University of Sheffield, Sheffield Children s, Hospital, Stephenson Wing, Sheffield, S10 2TH, UK
Cell Signal 20:1073-83. 2008..Thus, we demonstrate that cAMP/PKA/CnA signaling pathway is defective in CF cells and suggest that loss of anx 2-S100A10/CFTR complex formation may contribute to defective cAMP/PKA-dependent CFTR channel function... - Actin binding of ezrin is activated by specific recognition of PIP2-functionalized lipid bilayersMatthias Janke
Institute of Physical Chemistry, University of Mainz, Welder Weg 11, 55128 Mainz, Germany
Biochemistry 47:3762-9. 2008..Collectively, these data indicate that activation of ezrin can occur as a consequence of PIP 2 binding and does not require additional cofactors... - Retinosomes: new insights into intracellular managing of hydrophobic substances in lipid bodiesYoshikazu Imanishi
Department of Ophthalmology, University of Washington, Box 356485, Seattle, WA 98195-6485, USA
J Cell Biol 166:447-53. 2004..These observations suggest that lipid bodies, including retinosomes, carry out specific functions that go beyond those of mere lipid storage organelles... - Deletion of annexin 2 light chain p11 in nociceptors causes deficits in somatosensory coding and pain behaviorThomas Foulkes
Molecular Nociception Group, Department of Biology, University College London, London WC1E 6BT, United Kingdom
J Neurosci 26:10499-507. 2006..A significant deficit in neuropathic pain behavior was also apparent in the conditional-null mice. These results confirm an important role for p11 in nociceptor function...