Research Topics
| Gunther GerischSummaryCountry: Germany Publications
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Detail Information
Publications
Genetic evidence for concerted control of actin dynamics in cytokinesis, endocytic traffic, and cell motility by coronin and Aip1Hellen C Ishikawa-Ankerhold
Cell Dynamics, Max Planck Institute of Biochemistry, Martinsried, Germany
Cytoskeleton (Hoboken) 67:442-55. 2010..Our results prove that coronin and Aip1 are important effectors that act together in maintaining the balance of actin polymerization and depolymerization in living cells...
Curvature recognition and force generation in phagocytosisMargaret Clarke
Oklahoma Medical Research Foundation, Oklahoma City, OK 73121, USA
BMC Biol 8:154. 2010..We survey mechanisms that enable a phagocyte to remodel actin organization in response to particles of complex shape...
Different modes of state transitions determine pattern in the Phosphatidylinositide-Actin systemGunther Gerisch
Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
BMC Cell Biol 12:42. 2011..To study de-novo cell polarization, we first depolymerized actin and subsequently recorded the spontaneous reorganization of actin patterns in relation to PTEN...
Tubular-vesicular transformation in the contractile vacuole system of DictyosteliumGunther Gerisch
Max Planck Institut fur Biochemie, D 82152 Martinsried, Germany
Cell Biol Int 26:845-52. 2002..Proteins that control activities of this specialized organelle in Dictyostelium have been conserved during evolution and also regulate membrane trafficking in man...
Actin-binding proteins required for reliable chromosome segregation in mitosisGunther Gerisch
Max Planck Institut fur Biochemie, Martinsried, Germany
Cell Motil Cytoskeleton 57:18-25. 2004..We hypothesize that mutations in regulatory proteins of the actin network might be one cause of genetic instability of malignant tumor cells...
Mobile actin clusters and traveling waves in cells recovering from actin depolymerizationGunther Gerisch
Max Planck Institut fur Biochemie, D 82152 Martinsried, Germany
Biophys J 87:3493-503. 2004..These data show that the initiation of actin polymerization is separated in Dictyostelium from front protrusion, and that the coupling of polymerization to protrusion is a later step in the reconstitution of a leading edge...
GFP-golvesin constructs to study Golgi tubulation and post-Golgi vesicle dynamics in phagocytosisGunther Gerisch
Max Planck Institut fur Biochemie, Martinsried, Germany
Eur J Cell Biol 83:297-303. 2004..Applying this construct to a phagosome filled with multiple particles, we observed that the phagosome is segmented during exocytosis, meaning that sequential release of particles alternates with membrane fusion...
Toward the structure of dynamic membrane-anchored actin networks: an approach using cryo-electron tomographyGunther Gerisch
Max Planck Institut fur Biochemie, Martinsried, Germany
Cell Adh Migr 1:145-8. 2007....
Self-organizing actin waves as planar phagocytic cup structuresGunther Gerisch
Max Planck Institut fur Biochemie, Martinsried, Germany
Cell Adh Migr 3:373-82. 2009..We suggest that actin waves enable a phagocyte to scan a surface for particles that might be engulfed...
Self-organizing actin waves that simulate phagocytic cup structuresGunther Gerisch
Max Planck Institut fur Biochemie, Am Klopferspitz 18, D 82152 Martinsried, Germany
PMC Biophys 3:7. 2010..5) The actin wave and PIP3 patterns resemble 2-dimensional projections of phagocytic cups, suggesting that they are involved in the scanning of surfaces for particles to be taken up.PACS Codes: 87.16.Ln, 87.19.lp, 89.75.Fb...
Cytokinesis without myosin IIG Gerisch
Max Planck Institut fur Biochemie, Martinsried, D 82152, Germany
Curr Opin Cell Biol 12:126-32. 2000..A centripetal flow of actin filaments followed by their disassembly in the cleavage furrow is proposed to underlie the translocation...
Dynamic actin patterns and Arp2/3 assembly at the substrate-attached surface of motile cellsTill Bretschneider
Max Planck Institut fur Biochemie, D 82152, Martinsried, Germany
Curr Biol 14:1-10. 2004..The analysis of actin network dynamics requires the combination of high-resolution imaging with a specific fluorescent probe that highlights the filamentous actin structures in live cells...
Mechanically induced actin-mediated rocketing of phagosomesMargaret Clarke
Program in Molecular, Cell, and Developmental Biology, Oklahoma Medical Research Foundation, Oklahoma City, OK 73121, USA
Mol Biol Cell 17:4866-75. 2006..The forces generated by rocketing phagosomes are sufficient to push the entire microtubule apparatus forward and to dislocate the nucleus...
Endosome fusion and microtubule-based dynamics in the early endocytic pathway of dictyosteliumMargaret Clarke
Program in Molecular and Cell Biology, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma 73104, USA
Traffic 3:791-800. 2002..Further maturation of endosomes led to loss of pleiomorphy in favor of a spherical shape, inability to fuse with new macropinosomes, and diminished motility...
Dynamics of the vacuolar H(+)-ATPase in the contractile vacuole complex and the endosomal pathway of Dictyostelium cellsMargaret Clarke
Oklahoma Medical Research Foundation, Oklahoma City, USA
J Cell Sci 115:2893-905. 2002..VatM-GFP was retrieved from the phagosome membrane prior to exocytosis of the indigestible remnants of the yeast particle. These data suggest that vacuolar proton pumps are recycled by fusion of advanced with newly formed endosomes...
Retrieval of the vacuolar H-ATPase from phagosomes revealed by live cell imagingMargaret Clarke
Program in Genetic Models of Disease, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, United States of America
PLoS ONE 5:e8585. 2010..Similarly, the enzyme is thought to be retrieved from phagosome membranes prior to exocytosis of indigestible material, although that process has not been directly visualized...
The three-dimensional dynamics of actin waves, a model of cytoskeletal self-organizationTill Bretschneider
Max Planck Institut fur Biochemie, D 82152 Martinsried, Germany
Biophys J 96:2888-900. 2009..These data show that force-generating, highly organized supramolecular networks are autonomously formed in live cells from molecular motors and proteins controlling actin polymerization and depolymerization...
Time-resolved responses to chemoattractant, characteristic of the front and tail of Dictyostelium cellsMartin Etzrodt
Max Planck Institut fur Biochemie, Am Klopferspitz 18a, D 82152 Martinsried, Germany
FEBS Lett 580:6707-13. 2006..The cortical accumulation of myosin-II starts when the front response has declined, supporting the concept of divergent signal transmission and adaptation pathways...
Imaging actin cytoskeleton dynamics in Dictyostelium chemotaxisGunther Gerisch
Max Planck Institut fur Biochemie, Martinsried, Germany
Methods Mol Biol 571:385-400. 2009..Techniques of total internal reflection fluorescence (TIRF) and spinning-disc confocal microscopy provide appropriate temporal and spatial resolution for the analysis of actin dynamics...
Organization of actin networks in intact filopodiaOhad Medalia
Max Planck Institut fur Biochemie, D 82152 Martinsried, Germany
Curr Biol 17:79-84. 2007..We hypothesize that growth of the highly dynamic filopodia of Dictyostelium is accompanied by repetitive nucleation of actin polymerization at the filopod tip, followed by the rearrangement of filaments within the shaft...
Dictyostelium mutants lacking the cytoskeletal protein coronin are defective in cytokinesis and cell motilityE L de Hostos
Max Planck Institut fur Biochemie, Martinsried, Germany
J Cell Biol 120:163-73. 1993..This contrasts with the localization of myosin II in the cleavage furrow and suggests that coronin functions independently of the conventional myosin in facilitating cytokinesis...
Talin-null cells of Dictyostelium are strongly defective in adhesion to particle and substrate surfaces and slightly impaired in cytokinesisJ Niewöhner
Max Planck Institut fur Biochemie, D 82152 Martinsried, Germany
J Cell Biol 138:349-61. 1997..With their high proportion of binucleated cells, the talin-null mutants revealed interactions of the mitotic apparatus with the cell cortex that were not obvious in mononucleated cells...
Golvesin-GFP fusions as distinct markers for Golgi and post-Golgi vesicles in Dictyostelium cellsN Schneider
Max Planck lnstitut für Biochemie, Martinsried, Germany
Biol Cell 92:495-511. 2000..The fusion protein is retained in Golgi vesicles during mitosis, visualizing Golgi disassembly and reorganization in line with cytokinesis...
Chemoattractant-controlled accumulation of coronin at the leading edge of Dictyostelium cells monitored using a green fluorescent protein-coronin fusion proteinG Gerisch
Max Planck Institut fur Biochemie, Martinsried, Germany
Curr Biol 5:1280-5. 1995..It is a member of the WD-repeat family of proteins and is known to interact with actin-myosin complexes. In coronin null mutants, cell locomotion is slowed down and cytokinesis is impaired...
Coactosin interferes with the capping of actin filamentsU Röhrig
Max Planck Institut fur Biochemie, Martinsried, Germany
FEBS Lett 374:284-6. 1995....
Calreticulin and calnexin in the endoplasmic reticulum are important for phagocytosisA Müller-Taubenberger
Max Planck Institut fur Biochemie, D 82152 Martinsried, Germany
EMBO J 20:6772-82. 2001....
Phosphorylation of threonine residues on cloned fragments of the Dictyostelium myosin heavy chainG Wagle
Max Planck Institut fur Biochemie, Martinsried, FRG
FEBS Lett 227:71-5. 1988..Sites of phosphorylation were tentatively localized to a sequence in the C-terminal region of the heavy chain where four threonine residues are found...
Interaction of a Dictyostelium member of the plastin/fimbrin family with actin filaments and actin-myosin complexesJ Prassler
Max Planck Institut fur Biochemie, Martinsried, Germany
Mol Biol Cell 8:83-95. 1997..This result shows that myosin can disperse an actin network when it acts as a motor and sheds light on the dynamics of protein-protein interactions in the cortex of a motile cell where myosin II and Dd plastin are simultaneously present...
Cortexillins, major determinants of cell shape and size, are actin-bundling proteins with a parallel coiled-coil tailJ Faix
Max Planck Institut fur Biochemie, Martinsried, Federal Republic of Germany
Cell 86:631-42. 1996..The double-mutant cells are multinucleate owing to a severe impairment of cytokinesis...
Coactosin, a 17 kDa F-actin binding protein from Dictyostelium discoideumE L de Hostos
Max Planck Institut fur Biochemie, Martinsried, Germany
Cell Motil Cytoskeleton 26:181-91. 1993..The cDNA-derived amino-acid sequence reveals similarities of coactosin to the drebrins identified in neurons and to actin-binding proteins from other organisms, including yeast ABP1p, and yeast and vertebrate cofilins...
Drainin required for membrane fusion of the contractile vacuole in Dictyostelium is the prototype of a protein family also represented in manM Becker
Max Planck Institut fur Biochemie, D 82152 Martinsried, Germany
EMBO J 18:3305-16. 1999..We suggest that drainin acts in a signaling cascade that couples a volume-sensing device in the vacuolar membrane to the membrane fusion machinery...
Coronin, an actin binding protein of Dictyostelium discoideum localized to cell surface projections, has sequence similarities to G protein beta subunitsE L de Hostos
Max Planck Institut fur Biochemie, Martinsried, Germany
EMBO J 10:4097-104. 1991..It is hypothesized that coronin is implicated in the transmission of chemotactic signals from cAMP receptors in the plasma membrane through G proteins to the cortical cytoskeleton, whose structure and activity is locally modulated...
Proteasomes from Dictyostelium discoideum: characterization of structure and functionT M Schauer
Max Planck Institute for Biochemistry, Martinsried, Germany
J Struct Biol 111:135-47. 1993..The resulting model implies that the alpha- and beta-subunits have a fixed pattern of relationships. D. discoideum proteasomes are found both in the cytosol and, in higher concentrations, in the nucleus...
The hybrid histidine kinase DokA is part of the osmotic response system of DictyosteliumS C Schuster
Abteilung fur Membranebiochemie, Max Planck Institut fur Biochemie, 82152 Martinsried, Germany
EMBO J 15:3880-9. 1996..dokA is a member of a family of histidine kinase-like genes that play regulatory roles in eukaryotic cell function...
Classification of tyrosine kinases from Dictyostelium discoideum with two distinct, complete or incomplete catalytic domainsK Adler
Max Planck Institut fur Biochemie, Martinsried, Germany
FEBS Lett 395:286-92. 1996..discoideum, a branch of their own, separate from the tyrosine kinase domains in sensu strictu. Domain II in DPYK4 is found on a different branch close to serine/threonine kinases...
cDNA-derived sequence of UMP-CMP kinase from Dictyostelium discoideum and expression of the enzyme in Escherichia coliL Wiesmuller
Max Planck Institut fur Biochemie, Martinsried, Federal Republic of Germany
J Biol Chem 265:6339-45. 1990..05 and 0.1 microM) and is inactivated by modification of free thiol groups with 5,5'-dithiobis(2-nitrobenzoic acid)...
