Pete Gagnon

Summary

Publications

  1. ncbi An adaptation of hydrophobic interaction chromatography for estimation of protein solubility optima
    P Gagnon
    Validated Biosystems Incorporated, Tucson, AZ 85750, USA
    J Pharm Biomed Anal 16:587-92. 1997
  2. ncbi Monoclonal antibody purification with hydroxyapatite
    Pete Gagnon
    Validated Biosystems, 240 Avenida Vista Montana, Ste 7F, San Clemente, CA 92672, United States
    N Biotechnol 25:287-93. 2009
  3. ncbi Antibody aggregate removal by hydroxyapatite chromatography
    Pete Gagnon
    Validated Biosystems, San Clemente, CA 92672, USA
    Curr Pharm Biotechnol 10:440-6. 2009
  4. ncbi IgG aggregate removal by charged-hydrophobic mixed mode chromatography
    Pete Gagnon
    Validated Biosystems, San Clemente, CA 92672, USA
    Curr Pharm Biotechnol 10:434-9. 2009
  5. ncbi Cooperative multimodal retention of IgG, fragments, and aggregates on hydroxyapatite
    Pete Gagnon
    Validated Biosystems, San Clemente, CA 92672, USA
    J Sep Sci 32:3857-65. 2009
  6. ncbi Improved antibody aggregate removal by hydroxyapatite chromatography in the presence of polyethylene glycol
    Pete Gagnon
    Validated Biosystems, 240 Avenida Vista Montana, Suite 7F, San Clemente, CA 92672, USA
    J Immunol Methods 336:222-8. 2008
  7. ncbi Reverse calcium affinity purification of Fab with calcium derivatized hydroxyapatite
    Pete Gagnon
    Validated Biosystems, San Clemente, CA 92672, USA
    J Immunol Methods 342:115-8. 2009

Detail Information

Publications7

  1. ncbi An adaptation of hydrophobic interaction chromatography for estimation of protein solubility optima
    P Gagnon
    Validated Biosystems Incorporated, Tucson, AZ 85750, USA
    J Pharm Biomed Anal 16:587-92. 1997
    ..Cooperative effects of solubility modifiers such as amino acids can likewise be assessed. The technique can be a useful tool in the development of liquid formulations for protein pharmaceuticals...
  2. ncbi Monoclonal antibody purification with hydroxyapatite
    Pete Gagnon
    Validated Biosystems, 240 Avenida Vista Montana, Ste 7F, San Clemente, CA 92672, United States
    N Biotechnol 25:287-93. 2009
    ..Continuing development of novel elution strategies, alternative forms of HA, and application of robotic high throughput screening systems promise to expand HA's utility in the field...
  3. ncbi Antibody aggregate removal by hydroxyapatite chromatography
    Pete Gagnon
    Validated Biosystems, San Clemente, CA 92672, USA
    Curr Pharm Biotechnol 10:440-6. 2009
    ..Consideration is also given to HA's specific strengths and limitations from an industrial perspective...
  4. ncbi IgG aggregate removal by charged-hydrophobic mixed mode chromatography
    Pete Gagnon
    Validated Biosystems, San Clemente, CA 92672, USA
    Curr Pharm Biotechnol 10:434-9. 2009
    ..Consideration is also given to their specific strengths and limitations from an industrial perspective...
  5. ncbi Cooperative multimodal retention of IgG, fragments, and aggregates on hydroxyapatite
    Pete Gagnon
    Validated Biosystems, San Clemente, CA 92672, USA
    J Sep Sci 32:3857-65. 2009
    ..Electrostatic repulsion of amino residues and attraction of carboxyls by HA calcium appeared to be blocked by strong complexation of calcium with mobile phase phosphate...
  6. ncbi Improved antibody aggregate removal by hydroxyapatite chromatography in the presence of polyethylene glycol
    Pete Gagnon
    Validated Biosystems, 240 Avenida Vista Montana, Suite 7F, San Clemente, CA 92672, USA
    J Immunol Methods 336:222-8. 2008
    ..The HA method is suitable for preparation of aggregate-free IgG and IgM monoclonal antibodies for research, diagnostic, or therapeutic applications...
  7. ncbi Reverse calcium affinity purification of Fab with calcium derivatized hydroxyapatite
    Pete Gagnon
    Validated Biosystems, San Clemente, CA 92672, USA
    J Immunol Methods 342:115-8. 2009
    ..Alternatively, Fab can be concentrated on native hydroxyapatite then eluted selectively by conversion to the calcium-derivatized form...