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Genomes and Genes | Didier RaoultSummaryCountry: France Publications
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Publications
A new example of viral intein in MimivirusHiroyuki Ogata
Information Genomique et Structurale, UPR2589 CNRS, IBSM, IFR88, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
Virol J 2:8. 2005..After their discovery, inteins have been quickly identified in all domains of life, but only once to date in the genome of a eukaryote-infecting virus...
HIV-2 Protease resistance defined in yeast cellsNajoua Ben M'Barek
Unite des Rickettsies, Faculte de Medecine, 27 Bd Jean Moulin, 13385 Marseille Cedex 05, Pathologies Transmissibles et Pathologies Infectieuses Tropicales, IFR48, France
Retrovirology 3:58. 2006..In order to be able to define the specific mutations in the Protease that confer loss of susceptibility to Protease Inhibitors, we set up an experimental model system based in the expression of the viral protein in yeast...
Microarray for serotyping of Bartonella speciesCyrille J Bonhomme
Unite des Rickettsies, CNRS UMR 6020, Faculte de Medecine de Marseille, Marseille Cedex 05, France
BMC Microbiol 7:59. 2007..Bacteria of the genus Bartonella are responsible for a large variety of human and animal diseases. Serological typing of Bartonella is a method that can be used for differentiation and identification of Bartonella subspecies...
SVARAP and aSVARAP: simple tools for quantitative analysis of nucleotide and amino acid variability and primer selection for clinical microbiologyPhilippe Colson
Laboratoire de Virologie, Fédération Hospitalière de Bactériologie VirologieClinique et d Hygiène, CHRU Timone, 264 rue Saint Pierre 13385, Marseille Cedex 05, France
BMC Microbiol 6:21. 2006..Our aim was therefore to test easy and cost-free tools (SVARAP and aSVARAP) that require short hands-on work, little expertise, and which allow visual interpretation and statistical analysis of results...
Detection of "Rickettsia sp. strain Uilenbergi" and "Rickettsia sp. strain Davousti" in Amblyomma tholloni ticks from elephants in AfricaKotaro Matsumoto
Unite des Rickettsies, CNRS UMR 6020 IFR 48, Faculte de Medecine, Marseille, France
BMC Microbiol 7:74. 2007..To date, 6 tick-borne rickettsiae pathogenic for humans are known to occur in Africa and 4 of them were first identified in ticks before being recognized as human pathogens...
Multi-pathogens sequence containing plasmids as positive controls for universal detection of potential agents of bioterrorismRemy N Charrel
Unite des Rickettsies, CNRS UMR 6020 IFR 48, Faculte de Medecine, Marseille, France
BMC Microbiol 4:21. 2004..burnetii using constructed plasmids as positive controls...
Sca1, a previously undescribed paralog from autotransporter protein-encoding genes in Rickettsia speciesMaxime Ngwamidiba
Unite des Rickettsies, IFR 48, CNRS UMR 6020, Faculte de Medecine, Universite de la Mediterranee, 13385 Marseille Cedex 05, France
BMC Microbiol 6:12. 2006....
False positive PCR detection of Tropheryma whipplei in the saliva of healthy peopleJean Marc Rolain
Universite de la Mediterranee, Unite des Rickettsies, CNRS UMR 6020, IFR 48, Faculte de Medecine, Marseille Cedex 05, France
BMC Microbiol 7:48. 2007..whipplei in a series of 57 saliva from healthy people...
From cat scratch disease to endocarditis, the possible natural history of Bartonella henselae infectionFrédérique Gouriet
Unite des Rickettsies, CNRS UMR 6020, IFR 48, Faculte de Medecine, Universite de la Mediterranee, Marseille Cedex 05, France
BMC Infect Dis 7:30. 2007..To date, none of the reported patients have had a history of typical cat scratch disease (CSD) which is also a manifestation of infection with B. henselae...
Prosthetic valve endocarditis caused by Pseudomonas luteolaJean Paul Casalta
Laboratoire de Microbiologie, IFR 48, Centre Hospitalier Universitaire de la Timone, 264 rue Saint Pierre, Marseille, France
BMC Infect Dis 5:82. 2005..Pseudomonas luteola has been recognized as an uncommon cause of bacteremia and of infections in patients with underlying medical disorders...
Comparative genomic analysis of Tropheryma whipplei strains reveals that diversity among clinical isolates is mainly related to the WiSP proteinsMy Van La
Unite des Rickettsies, CNRS UMR6020, IFR48, Faculte de Medecine, 27 Bd Jean Moulin, Marseille, F13385, France
BMC Genomics 8:349. 2007..whipplei Twist strain. For each isolate, the genes were defined as either present or absent/divergent using the GACK analysis software. Genomic changes were then further characterized by PCR and sequencing...
A flea-associated Rickettsia pathogenic for humansD Raoult
Unite des Rickettsies, CNRS UPRESA 6020, Faculte de Medecine, Universite de la Mediterranee, 27 Bd Jean Moulin, 13385 Marseille, France
Emerg Infect Dis 7:73-81. 2001..Our successful isolation of this rickettsia makes it available for use in serologic tests to determine its clinical spectrum, prevalence, and distribution...
Natural history and pathophysiology of Q feverD Raoult
Unite des Rickettsies, CNRS UMR 6020, Faculte de Medecine, Universite de la Mediterranee, Marseille, France
Lancet Infect Dis 5:219-26. 2005..Advances in understanding the pathophysiology and natural history of Q fever are reviewed...
What does the future hold for clinical microbiology?Didier Raoult
Unite des Rickettsies, CNRS UMR 6020, Faculte de Medecine, Universite de la Mediterranee, 27 Bd Jean Moulin, 13385 Marseille, France
Nat Rev Microbiol 2:151-9. 2004....
First isolation of Bartonella alsatica from a valve of a patient with endocarditisDidier Raoult
Universite de la Mediterranee, Unite des Rickettsies, CNRS UMR 6020, IFR 48, Faculte de Medecine, Marseille, France
J Clin Microbiol 44:278-9. 2006..B. alsatica was identified by serology and culture and by PCR of an aortic valve specimen. B. alsatica should be added to the list of zoonotic agents of blood culture-negative endocarditis...
Nanobacteria are mineralo fetuin complexesDidier Raoult
Unite des Rickettsies, Centre National de la Recherche Scientifique UMR 6020, IFR 48, Faculte de Medecine, Marseille, France
PLoS Pathog 4:e41. 2008..quot; The presence of fetuin within renal calculi was also evidenced, suggesting its role as a hydroxyapatite nucleating factor...
Infections in the homelessD Raoult
Unite des Rickettsies, Universite de la Mediterranee, Faculte de Medecine, and Hĵpital Nord Service des Maladies Infectieuses et Tropicales, Marseille, France
Lancet Infect Dis 1:77-84. 2001..Patients with serious and contagious illnesses should be hospitalised. Physicians should be aware of these specific issues to enhance care...
Q fever during pregnancy: diagnosis, treatment, and follow-upDidier Raoult
Unite des Rickettsies, Universite de la Mediterranee, Faculte de Medecine, CNRS UMR 6020, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
Arch Intern Med 162:701-4. 2002..Q fever, caused by Coxiella burnetii, may result in abortions, premature deliveries, and stillbirths in infected pregnant women...
Lack of correlation between Bartonella DNA detection within fleas, serological results, and results of blood culture in a Bartonella-infected stray cat populationB La Scola
Unite des Rickettsies, CNRS UPRESA 6020, Faculte de Medecine, Marseille, France
Clin Microbiol Infect 8:345-51. 2002..To correlate the presence of different Bartonella species in the blood of a stray cat population trapped on a French military base with specific antibodies and species detected in cat fleas...
Outcome and treatment of Bartonella endocarditisDidier Raoult
Unite des Rickettsies, Faculte de Medecine, Universite de la Mediterranee, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
Arch Intern Med 163:226-30. 2003..Endocarditis caused by Bartonella species is a potentially lethal infection characterized by a subacute evolution and severe valvular lesions...
Spotless rickettsiosis caused by Rickettsia slovaca and associated with Dermacentor ticksDidier Raoult
Unite des Rickettsies, Faculte de Medecine, Universite de la Mediterranee, 13385 Marseille Cedex 05, France
Clin Infect Dis 34:1331-6. 2002..Immunofluorescence and/or Western blot analysis detected antibodies in 50% of tested patients. Three Dermacentor ticks obtained from patients revealed R. slovaca by PCR...
Treatment of Q fever endocarditis: comparison of 2 regimens containing doxycycline and ofloxacin or hydroxychloroquineD Raoult
Unite des Rickettsies, Faculte de Medecine, Université de la Mediterranée CNRS, Marseille, France
Arch Intern Med 159:167-73. 1999..Q fever endocarditis, caused by Coxiella burnetii, is fatal in 25% to 60% of patients. Currently, treatment with a long-term tetracycline and quinolone regimen for at least 4 years is recommended, although relapses are frequent...
Contribution of systematic serological testing in diagnosis of infective endocarditisD Raoult
Unite des Rickettsies, Hopital de la Timone, Faculte de Medicine, Universite de la Mediterranee, 13385 Marseille Cedex 05, France
J Clin Microbiol 43:5238-42. 2005..9%) possible cases of IE as definite cases. Systematic serological testing improved the performance of the modified Duke criteria and was instrumental in establishing the etiologic diagnosis in 8% (34/427) cases of IE...
Purification of Encephalitozoon cultures contaminated by mycoplasmas by murine intraperitoneal inoculationO Ridoux
Unité des Rickettsies CNRS UPRES A 6020, Faculte de Medecine, Universite de la Mediterranee, Marseille, France
J Clin Microbiol 36:2380-2. 1998..Mycoplasma-free microsporidia were isolated from the brains and spleens of inoculated mice 24 h postinoculation by using the centrifugation shell vial system. Identification was confirmed by direct sequencing of PCR-amplified 16S rRNA...
A pedagogical farm as a source of Q fever in a French cityHervé Tissot-Dupont
Faculte de Medecine, Unite des Rickettsies, CNRS UMR 6020, Marseille, France
Eur J Epidemiol 20:957-61. 2005..These farms require a drastic hygiene and control. Q fever, as a public health problem, still requires further studies to identify and confirm individual behavioral risk factors...
The 1.2-megabase genome sequence of MimivirusDidier Raoult
Unite des Rickettsies, Faculte de Medecine, CNRS UMR6020, Universite de la Mediterranee, 13385 Marseille Cedex 05, France
Science 306:1344-50. 2004..This new sequence data might help shed a new light on the origin of DNA viruses and their role in the early evolution of eukaryotes...
Evidence for louse-transmitted diseases in soldiers of Napoleon's Grand Army in VilniusDidier Raoult
Unite des Rickettsies, Centre National de la Recherche Scientifique CNRS Unite Mixte de Recherche UMR 6020, Universite de la Mediterranee, Faculte de Medecine, Marseille, France
J Infect Dis 193:112-20. 2006..Because soldiers were commonly infested with body lice, it has been speculated that louse-borne infectious diseases, such as epidemic typhus (caused by Rickettsia prowazekii), were common...
Survey of three bacterial louse-associated diseases among rural Andean communities in Peru: prevalence of epidemic typhus, trench fever, and relapsing feverD Raoult
Unite des Rickettsies, Faculte de Medecine, Universite de la Mediterranee, Marseille, France
Clin Infect Dis 29:434-6. 1999..quintana DNA was amplified from lice. Epidemic typhus remains extant in the area, and B. quintana infections were encountered and documented for the first time in South America...
The body louse as a vector of reemerging human diseasesD Raoult
Universite de la Mediterranee, Faculte de Medecine, Centre National de la Recherche Scientifique, Unité Propre de Recherche de l Enseignement Superieur Associée 6020, Marseille, France
Clin Infect Dis 29:888-911. 1999..A small outbreak was also reported in Russia in 1997. Louse infestation appears to become more prevalent worldwide, associated with a decline in social and hygienic conditions provoked by civil unrest and economic instability...
Cultivation of the bacillus of Whipple's diseaseD Raoult
Unite des Rickettsies, Universite de la Mediterranee, Faculte de Medecine, Marseilles, France
N Engl J Med 342:620-5. 2000..Whipple's disease is a systemic bacterial infection, but to date no isolate of the bacterium has been established in subculture, and no strain of this bacterium has been available for study...
Naming of Rickettsiae and rickettsial diseasesDidier Raoult
Unite des Rickettsies, IFR 48, CNRS UMR 6020, Faculte de Medecine, Universite de la Mediterranee, 13385 Marseille Cedex 05, France
Ann N Y Acad Sci 1063:1-12. 2005..In this paper, we review taxonomic rules and appropriate approaches to valid naming of rickettsial species and the diseases they cause...
Q fever 1985-1998. Clinical and epidemiologic features of 1,383 infectionsD Raoult
Unite des Rickettsies, Universite de la Mediterranee, Marseille, France
Medicine (Baltimore) 79:109-23. 2000..Our data confirm that chronic Q fever is mainly determined by host factors and demonstrate for the first time that host factors may also play a role in the clinical expression of acute Q fever...
Molecular genetic methods for the diagnosis of fastidious microorganismsFlorence Fenollar
, , , Marseille, France
APMIS 112:785-807. 2004..Ehrlichia spp., Coxiella burnetii, Bartonella spp., Tropheryma whipplei and Yersinia pestis...
Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogensHiroyuki Ogata
Structural and Genomic Information Laboratory, Centre National de la Recherche Scientifique UPR 2589, Institut de Biologie Structurale et Microbiologie, Parc Scientifique de Luminy, Marseille, France
PLoS Genet 2:e76. 2006....
Rickettsia tamurae sp. nov., isolated from Amblyomma testudinarium ticksPierre Edouard Fournier
Unite des Rickettsies, IFR 48, CNRS UMR 6020, Faculte de Medecine, Universite de la Mediterranee, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
Int J Syst Evol Microbiol 56:1673-5. 2006..Such genotypic and phenotypic characteristics warrant its classification as a representative of a novel species, for which the name Rickettsia tamurae sp. nov. is proposed, with the type strain AT-1(T) (=CSUR R1(T))...
Coxiella burnetii in humans and ticks in rural SenegalOleg Mediannikov
URMITE, UMR IRD 198 CNRS 6236, Mediterranean University, Medical Faculty, Marseille, France
PLoS Negl Trop Dis 4:e654. 2010..Q fever is a worldwide zoonotic disease caused by Coxiella burnetii. Epidemiologically, animals are considered reservoirs and humans incidental hosts...
rpoB gene sequence-based characterization of emerging non-tuberculous mycobacteria with descriptions of Mycobacterium bolletii sp. nov., Mycobacterium phocaicum sp. nov. and Mycobacterium aubagnense sp. novToidi Adekambi
Unite des Rickettsies, CNRS UMR 6020 IFR 48, Faculte de Medecine, 27, Boulevard Jean Moulin, Université de la Méditerranée and Assistance Publique Hôpitaux de Marseille Timone, Fédération de Microbiologie Clinique, Marseille, France
Int J Syst Evol Microbiol 56:133-43. 2006....
Rapid comparative genomic analysis for clinical microbiology: the Francisella tularensis paradigmBernard La Scola
Unite des Rickettsies CNRS UMR 6020, IFR 48, Faculte de Medecine de Marseille, Universite de la Mediterranee, 13385 Marseille Cedex 05, France
Genome Res 18:742-50. 2008..In the bioterrorism context, it allows the rapid detection of strain manipulation, including intentionally added virulence genes and genes that support antibiotic resistance...
Analysis of the Rickettsia africae genome reveals that virulence acquisition in Rickettsia species may be explained by genome reductionPierre Edouard Fournier
Unite des Rickettsies, IFR 48 CNRS UMR 6020, Faculte de Medecine, Universite de la Mediterranee, 27 Boulevard Jean Moulin, Marseille Cedex 05, France
BMC Genomics 10:166. 2009..prowazekii, which causes epidemic typhus and kills its arthropod host, to the mild pathogen R. africae, the agent of African tick-bite fever, which does not affect the fitness of its tick vector...
Genomic analysis of an emerging multiresistant Staphylococcus aureus strain rapidly spreading in cystic fibrosis patients revealed the presence of an antibiotic inducible bacteriophageJean Marc Rolain
URMITE CNRS IRD UMR 6236, Faculte de Medecine et de Pharmacie, Universite de la Mediterranee, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
Biol Direct 4:1. 2009..We also conducted a retrospective epidemiological analysis on all S. aureus isolated from 2002 to 2007 in CF patients from our institution...
Staphylococcus massiliensis sp. nov., isolated from a human brain abscessMouhamad Al Masalma
Laboratoire de Bacteriologie virologie, Hopital de la Timone, URMITE CNRS IRD UMR 6236, IFR48, 264 rue Saint Pierre, 13385 Marseille, Cedex 05, France
Int J Syst Evol Microbiol 60:1066-72. 2010..8 %). On the basis of phenotypic and genotypic data, this isolate represents a novel species for which the name Staphylococcus massiliensis sp. nov. is proposed (type strain 5402776(T)=CCUG 55927(T)=CSUR P23(T))...
Detection of a rickettsia closely related to Rickettsia aeschlimannii, "Rickettsia heilongjiangensis," Rickettsia sp. strain RpA4, and Ehrlichia muris in ticks collected in Russia and KazakhstanStanislav Shpynov
Unite des Rickettsies, IFR48, CNRS UMR 6020, Faculte de Medecine, Universite de la Mediterranee, 13385 Marseille Cedex 5, France
J Clin Microbiol 42:2221-3. 2004..In Kazakhstan, we detected Rickettsia sp. strain RpA4 and a rickettsia closely related to Rickettsia aeschlimannii. These agents should be considered in a differential diagnosis of tick-borne infections in these areas...
Persistent Coxiella burnetii infection in mice overexpressing IL-10: an efficient model for chronic Q fever pathogenesisSoraya Meghari
Unite des Rickettsies, CNRS UMR 6020, Institut Federatif de Recherche 48, Universite de la Mediterranee, Faculte de Medecine, Marseille, France
PLoS Pathog 4:e23. 2008..To our knowledge, this is the first efficient model for chronic Q fever pathogenesis...
Reductive genome evolution from the mother of RickettsiaGuillaume Blanc
Structural and Genomic Information Laboratory, Institut de Biologie Structurale et Microbiologie, Parc Scientifique de Luminy, Marseille, France
PLoS Genet 3:e14. 2007....
Outbreak of Corynebacterium pseudodiphtheriticum infection in cystic fibrosis patients, FranceFadi Bittar
Universite de la Mediterranee, Marseille, France
Emerg Infect Dis 16:1231-6. 2010..Our study reemphasizes the conclusion that correctly identifying bacteria at the species level facilitates detection of an outbreak of new or emerging infections in humans...
Finegoldia magna, an early post-operative cause of infectious endocarditis: report of two cases and review of the literaturePierre Edouard Fournier
Fédération de Microbiologie, Hopital de la Timone, Marseille Hôpital de la Timone, rue Saint Pierre, 13385 Marseille Cedex 5, France
Anaerobe 14:310-2. 2008..Our report highlights the potential role of F. magna in early post-surgical endocarditis on prosthetic valves...
A murine model of infection with Rickettsia prowazekii: implications for pathogenesis of epidemic typhusYassina Bechah
Unite des Rickettsies, CNRS UMR 6020, Universite de la Mediterranee, Faculte de Medecine, 27 Bld J Moulin, 13385 Marseille Cedex 05, France
Microbes Infect 9:898-906. 2007..We concluded that the BALB/c mouse strain provides a useful model for studying the pathogenic mechanisms of epidemic typhus and its control by the immune system...
Identification of two putative rickettsial adhesins by proteomic analysisPatricia Renesto
Unite des Rickettsies, CNRS UMR 6020, IFR 48, Faculte de Medecine, 27 Boulevard Jean Moulin, 13385 Marseille, France
Res Microbiol 157:605-12. 2006..Both proteins are highly conserved within the Rickettsia genus and might play a critical role in their pathogenicity. These data may have important implications for the development of future vaccines against rickettsial infections...
Novel virus influenza A (H1N1sw) in South-Eastern France, April-August 2009Antoine Nougairede
Unité Mixte de Recherche 190 Unité des Virus Emergents, Université de la Méditerranée et Institut de Recherche pour le Développement, Marseille, France
PLoS ONE 5:e9214. 2010..In April 2009, the first cases of pandemic (H1N1)-2009 influenza [H1N1sw] virus were detected in France. Virological surveillance was undertaken in reference laboratories of the seven French Defence Zones...
Emended description of Rickettsia felis (Bouyer et al. 2001), a temperature-dependent cultured bacteriumBernard La Scola
Unite des Rickettsies, CNRS UPRESA 6020, Faculte de Medecine, Universite de la Mediterranee, 27 Bd Jean Moulin, 13385 Marseille 05, France
Int J Syst Evol Microbiol 52:2035-41. 2002..felis and those observed for the isolate in this study indicated some differences, although concurrent analysis of the two was not possible as no extant isolates of the first isolate of R. felis exist...
Multispacer typing to study the genotypic distribution of Bartonella henselae populationsWenjun Li
, IFR 48, CNRS UMR 6020, , , 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
J Clin Microbiol 44:2499-506. 2006..Finally, we provide a free-access MST-Rick online software program (http://ifr48.timone.univ-mrs.fr/MST_BHenselae/mst) that investigators may use to compare their own MST sequences to our database...
Molecular detection of Bartonella spp. in the dental pulp of stray cats buried for a yearGerard Aboudharam
Unite des Rickettsies, CNRS UMR 6020, IFR 48, Faculte de Medecine, Universite de la Mediterranee, 27, Boulevard Jean Moulin, Marseille Cedex 05 13385, France
Microb Pathog 38:47-51. 2005..grahamii (one cat). Our study indicates that dental pulp from the teeth of cats, especially the canine teeth, may be used for the PCR detection of Bartonella in animals buried for a year...
MALDI-TOF-mass spectrometry applications in clinical microbiologyPiseth Seng
Pôle des Maladies Infectieuses, Assistance Publique Hôpitaux de Marseille et URMITE UMR CNRS IRD 6236, IFR48, Faculte de Medecine, Universite de la Mediterranee, Marseille, France
Future Microbiol 5:1733-54. 2010..We will also discuss the application of MALDI-TOF-MS tools in the identification of Archaea, eukaryotes and viruses. Pathogenic identification from colony-cultured, blood-cultured, urine and environmental samples is also reviewed...
Isolation of a rickettsia related to Astrakhan fever rickettsia from a patient in ChadPierre Edouard Fournier
Unite des Rickettsies, IFR 48, CNRS UMR 6020, Faculte de Medecine, Universite de la Mediterranee, 13385 Marseilles Cedex 05, France
Ann N Y Acad Sci 990:152-7. 2003..Although Astrakhan fever rickettsia is transmitted by Rhipicephalus ticks in Europe, further studies are indicated to identify its vector in Africa where these ticks are also prevalent...
TLR2 is necessary to inflammatory response in Coxiella burnetii infectionSoraya Meghari
Unité des Rickerrsies, CNRS UMR 6020, Université de Méditerranée, 13005 Marseille, France
Ann N Y Acad Sci 1063:161-6. 2005..These results show that TLR2 is required for inflammatory and immune response to C. burnetii, but is dispensable for bacterial clearance...
Molecular identification of a collection of spotted Fever group rickettsiae obtained from patients and ticks from RussiaStanislav N Shpynov
Unite des Rickettsies, Faculte de Medecine, Marseille, France
Am J Trop Med Hyg 74:440-3. 2006..Clinicians should be aware that several spotted fever rickettsioses with different prognoses coexist in Russia in areas where NATT was the only previously recognized rickettsiosis...
Pig liver sausage as a source of hepatitis E virus transmission to humansPhilippe Colson
Pôle des Maladies Infectieuses et Tropicales Clinique et Biologique, Fédération de Bactériologie Hygiène Virologie, Centre Hospitalo Universitaire Timone, Marseille, France
J Infect Dis 202:825-34. 2010..We investigated the role of figatellu, a traditional pig liver sausage widely eaten in France and commonly consumed raw, as a source of HEV infection...
Frequent transmission of hepatitis E virus among piglets in farms in Southern FranceMamadou Kaba
URMITE CNRS IRD UMR 6236, Facultes de Medecine et de Pharmacie, Universite de la Mediterranee Aix Marseille II, Marseille, France
J Med Virol 81:1750-9. 2009..The present data indicate that 3-month-old pigs from Southern France might represent a potential source of HEV transmission to humans, and stress the potential of HEV to cause epizootic infections in population of farm pigs...
Investigation of blood culture-negative early prosthetic valve endocarditis reveals high prevalence of fungiFranck Thuny
Unite de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, CNRS IRD UMR 6236, Faculte de Medecine, Universite de la Mediterranee, 27 Blvd Jean Moulin, 13385 Marseille Cedex 05, France
Heart 96:743-7. 2010..Therefore, fungi should be investigated particularly by molecular methods on surgical specimens and an antifungal drug might be added to the empiric treatment...
Rickettsia conorii transcriptional response within inoculation escharPatricia Renesto
Unite des Rickettsies, IRD CNRS UMR 6236, Faculte de Medecine, Marseille, France
PLoS ONE 3:e3681. 2008..The skin thus constitutes an important barrier for the entry and propagation of R. conorii. Given this, analysis of the survival strategies used by the bacterium within infected skin is critical for our understanding of rickettsiosis...
Genotyping, Orientalis-like Yersinia pestis, and plague pandemicsMichel Drancourt
, Marseille, France
Emerg Infect Dis 10:1585-92. 2004..pestis Orientalis. These data indicate that Y. pestis caused cases of Justinian plague. The two historical plague pandemics were likely caused by Orientalis-like strains...
The transcriptional programme of human heart valves reveals the natural history of infective endocarditisMarie Benoit
Unité de Recherche sur les Maladies Infectieuses Transmissibles et Emergentes, Faculte de Medecine, Centre National de la Recherche Scientifique Unité Mixte de Recherche 6236, Universite de la Mediterranee, Marseille, France
PLoS ONE 5:e8939. 2010..Some parameters identified in this work could become tools for measuring the disease activity and should be tested as biomarkers for diagnosis or prognosis assessment in future studies...
Proposal to create subspecies of Rickettsia conorii based on multi-locus sequence typing and an emended description of Rickettsia conoriiYong Zhu
Unite des Rickettsies, IFR 48, CNRS UMR 6020, Faculte de Medecine, Universite de la Mediterranee, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
BMC Microbiol 5:11. 2005..Then, we selected a representative of each MLST genotype and used multi-spacer typing (MST) and mouse serotyping to estimate their degree of taxonomic relatedness...
Cardiac valves in patients with Whipple endocarditis: microbiological, molecular, quantitative histologic, and immunohistochemical studies of 5 patientsHubert Lepidi
Unite des Rickettsies, Centre National de la Recherche Scientifique, Unite de Mixte de Recherche 6020, Institut Federatif Recherche 48, Faculte de Medecine, Universite de la Mediterranee, Marseille, France
J Infect Dis 190:935-45. 2004..We report, for the first time, the immunodetection of T. whipplei in a surgically removed arterial embolus. Pathological and immunohistologic analyses may contribute to the diagnosis of Whipple endocarditis...
Actinomyces massiliensis sp. nov., isolated from a patient blood cultureAurelie Renvoise
Laboratoire de Bacteriologie virologie, Hopital de la Timone, CNRS UMR 6236, CNRS IRD, IFR48, 264 rue Saint Pierre, 13385 Marseille Cedex 05, France
Int J Syst Evol Microbiol 59:540-4. 2009..On the basis of phenotypic data and phylogenetic inference, the novel species Actinomyces massiliensis sp. nov. is proposed; the type strain is 4401292(T) (=CSUR P18(T)=CCUG 53522(T))...
Lipopolysaccharide from Coxiella burnetii is involved in bacterial phagocytosis, filamentous actin reorganization, and inflammatory responses through Toll-like receptor 4Amélie Honstettre
Unite des Rickettsies, Centre National de la Recherche Scientifique Unité Mixte de Recherche 6020, Institut Federatif de Recherche 48 Université de la Méditerranée, Marseille, France
J Immunol 172:3695-703. 2004..Taken together, these results demonstrate that TLR4 controls early events of C. burnetii infection such as macrophage phagocytosis, granuloma formation, and cytokine production...
Value of Tropheryma whipplei quantitative polymerase chain reaction assay for the diagnosis of Whipple disease: usefulness of saliva and stool specimens for first-line screeningFlorence Fenollar
Unite des Rickettsies, Universite de la Mediterranee, Centre National de Recherche Scientifique, Marseille, France
Clin Infect Dis 47:659-67. 2008..Whipple disease (WD) is a chronic infectious disease caused by Tropheryma whipplei. WD DNA has been found in stool and saliva specimens from patients and asymptomatic carriers...
Intracellular life of Coxiella burnetii in macrophagesEric Ghigo
URMITE CNRS UMR 6236 IRD 3R198, Institut Federatif de Recherche 48, Universite de la Mediterranee, Marseille, France
Ann N Y Acad Sci 1166:55-66. 2009..burnetii also depends on the functional state of myeloid cells. This review describes the mechanisms used by C. burnetii to circumvent uptake and trafficking events, and the role of cytokines on C. burnetii survival in myeloid cells...
Heterogeneity of susceptibility to fluoroquinolones in Bartonella isolates from Australia reveals a natural mutation in gyrAEmmanouil Angelakis
Unite des Rickettsies, CNRS UMR 6020, IFR 48, Faculté de Médecine et de Pharmacie Université de la Méditerranée, 27 Bd Jean Moulin, 13385 Marseille Cedex 05, France
J Antimicrob Chemother 61:1252-5. 2008..Our objective was to evaluate in vitro antibiotic susceptibilities of 20 new Bartonella isolates from animals in Australia...
Coxiella burnetii infection in C57BL/6 mice aged 1 or 14 monthsMarc Leone
Unite des Rickettsies, Centre National de la Recherche Scientifique, Universite de la Mediterranee, Faculte de Medecine, Marseille, France
FEMS Immunol Med Microbiol 50:396-400. 2007..In addition, macrophage microbicidal competence was similar in mature and young mice. These results suggest the importance of individual host factors in the pathophysiology of an infectious disease such as Q fever...
Lymph node biopsy specimens and diagnosis of cat-scratch diseaseJean-Marc Rolain
, Marseille, France
Emerg Infect Dis 12:1338-44. 2006..Histologic analysis of lymph node biopsy specimens should be routinely performed because some patients might have a concurrent malignant disease or mycobacteriosis...
Proteome analysis of Rickettsia conorii by two-dimensional gel electrophoresis coupled with mass spectrometryPatricia Renesto
Unite des Rickettsies, CNRS UMR 6020, IFR 48, Faculte de Medecine, 27 Boulevard Jean Moulin, 13385 Marseille, France
FEMS Microbiol Lett 245:231-8. 2005..conorii, to compare protein profiles of different isolates or of bacteria maintained under different experimental conditions and to identify immunogenic proteins as potential vaccine targets...
Coxiella burnetii stimulates production of RANTES and MCP-1 by mononuclear cells: modulation by adhesion to endothelial cells and its implication in Q feverSoraya Meghari
, CNRS UMR 6020, , , , 27 Bd Jean Moulin, 13385 Marseille Cedex 5, France
Eur Cytokine Netw 17:253-9. 2006..We suggest that the overproduction of RANTES and MCP-1 secondary to the contact of PBMC with endothelium may perpetuate exaggerated inflammatory responses leading to inappropriate PBMC trafficking and to the pathogenesis of Q fever...
Yersinia pestis Orientalis in remains of ancient plague patientsMichel Drancourt
Universite de la Mediterranee, Marseille, France
Emerg Infect Dis 13:332-3. 2007..We have now sequenced Y pestis glpD gene in such remains, showing a 93-bp deletion specific for biotype Orientalis. These data show that only Orientalis type caused the 3 plague pandemics...
First molecular evidence of new Bartonella spp. in fleas and a tick from PeruPhilippe Parola
, , , Marseille, France
Am J Trop Med Hyg 67:135-6. 2002..One of the genotypes is closely related to B. vinsoni subsp. berkhoffii, and the others seem to originate from unknown Bartonella species, whose medical importance has yet to be clarified...
Body lice as tools for diagnosis and surveillance of reemerging diseasesV Roux
, CNRS UPRES A 6020, , 13385 Marseille, France
J Clin Microbiol 37:596-9. 1999....
Genotypic characteristics of two serotypes of Bartonella henselaeBernard La Scola
, CNRS UMR 6020, 13385 Marseille Cedex 5, France
J Clin Microbiol 40:2002-8. 2002..Study of 22 additional strains of B. henselae isolated from French bacteremic cats demonstrated that they all belong to one or the other of the proposed serotype or genotype...
Use of highly variable intergenic spacer sequences for multispacer typing of Rickettsia conorii strainsPierre-Edouard Fournier
, IFR 48, CNRS UMR 6020, , , 27 Blvd. Jean Moulin, 13385 Marseille Cedex 5, France
J Clin Microbiol 42:5757-66. 2004..conorii isolates. Our study supports the usefulness of MST for strain genotyping. This tool may be useful for tracing a strain and identifying its source during outbreaks, including those resulting from bioterrorism...
Genetic identification of rickettsiae isolated from ticks in JapanPierre Edouard Fournier
Unite des Rickettsies, Faculte de Medecine, CNRS UMR 6020 27, Universite de la Mediterranee, 13385 Marseille, Cedex 05, France
J Clin Microbiol 40:2176-81. 2002..Finally, we identified a new genotype of unknown pathogenicity, genotype AT, that was isolated from Amblyomma testudinarium ticks and that is closely related to a Slovakian genotype obtained from Ixodes ricinus ticks...
[Molecular tools in the epidemiology of tick-borne bacterial diseases]P Parola
, CNRS UMR 6020, , 27, Bd Jean Moulin, 13385 Marseille Cedex 5, France
Ann Biol Clin (Paris) 59:177-82. 2001..Anaplasma marginale the agent of bovine anaplasmosis was detected in Mali. Rickettsiae of unknown pathogenicity were detected in Mali and Niger...
Bacteroides massiliensis sp. nov., isolated from blood culture of a newbornLukas Fenner
, CNRS UPRESA 6020, , , Marseille, France
Int J Syst Evol Microbiol 55:1335-7. 2005..8 % 16S rRNA similarity to Bacteroides vulgatus and a DNA G + C content of 49 mol%. The major cellular fatty acid is anteiso-branched C(15 : 0). The type strain of B. massiliensis sp. nov. is B84634(T) (=CIP 107942(T) = CCUG 48901(T))...
Q Fever during pregnancy: a cause of poor fetal and maternal outcomeXavier Carcopino
Service de Gynecologie Obstetrique, Hopital Nord, Chemin des Bourrely, 13915 cedex 20, Marseille, France
Ann N Y Acad Sci 1166:79-89. 2009..Such treatment should be used to treat pregnant women with Q fever. Women with previous history of Q fever should have a regular serological follow up. Obstetricians' knowledge about Q fever must be improved...
Validation of partial rpoB gene sequence analysis for the identification of clinically important and emerging Acinetobacter speciesVijay A K B Gundi
Unite des Rickettsies, CNRS UMR 6236, Faculte de Medecine de Marseille, 13385 Marseille Cedex 05, France
Microbiology 155:2333-41. 2009..Our data emphasize the recognition of A. schindleri as an emerging cause of Acinetobacter-related infection and confirm that A. gen. sp. 3 is the second most commonly isolated Acinetobacter species after A. baumannii in patients...
Massive comparative genomic analysis reveals convergent evolution of specialized bacteriaVicky Merhej
Faculty of Medicine, Unit for Research on Emergent and Tropical Infectious Diseases, CNRS IRD UMR 6236 IFR48, University of the Mediterranean, Marseilles, France
Biol Direct 4:13. 2009..The growing number of available sequenced genomes makes it possible to perform a statistical comparative analysis of 317 genomes from bacteria with different lifestyles...
Immunofluorescent detection of intraerythrocytic Bartonella henselae in naturally infected catsJ M Rolain
, CNRS UMR 6020, , 13385 Marseille Cedex 05, France
J Clin Microbiol 39:2978-80. 2001..Three cats that were positive by blood culture were also positive by direct immunofluorescence, and laser confocal microscopy confirmed the intraerythrocytic location of B. henselae...
Bartonella quintana in a 4000-year-old human toothMichel Drancourt
Unite des Rickettsies, CNR UMR 6020, IFR 48, Marseille, France
J Infect Dis 191:607-11. 2005..Humans are the reservoir of B. quintana, the louse-borne agent of trench fever. We detected DNA of B. quintana in the dental pulp of a person who died 4000 years ago...
Bosea eneae sp. nov., Bosea massiliensis sp. nov. and Bosea vestrisii sp. nov., isolated from hospital water supplies, and emendation of the genus Bosea (Das et al. 1996)Bernard La Scola
, CNRS UPRESA 6020, , , 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
Int J Syst Evol Microbiol 53:15-20. 2003..For treatment of infections caused by these novel bacteria, doxycycline appears to be the sole antibacterial compound with a consistently low MIC value...
PCR detection of bacteria on cardiac valves of patients with treated bacterial endocarditisClarisse Rovery
, , , , Marseille, France
J Clin Microbiol 43:163-7. 2005....
Gene repertoire of amoeba-associated giant virusesPhilippe Colson
URMITE UMR CNRS 6236 IRD 198, Facultes de Medecine et de Pharmacie, Universite de la Mediterranee, Marseille, France
Intervirology 53:330-43. 2010..Altogether, the data so far accumulated on amoeba-associated giant viruses are a powerful incentive to isolate and study additional strains to gain better understanding of their pangenome...
Blood culture-negative endocarditis in a reference center: etiologic diagnosis of 348 casesPierre Houpikian
, , , CNRS UPRES A 6020, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
Medicine (Baltimore) 84:162-73. 2005..Further investigations should be focused on this group to identify new agents of infective endocarditis...
Value of microimmunofluorescence for diagnosis and follow-up of Bartonella endocarditisPierre-Edouard Fournier
, IFR 48 CNRS, , , 13385 Marseille Cedex 05, France
Clin Diagn Lab Immunol 9:795-801. 2002..We propose that this criterion, which may also help with the detection of late relapses, be included as a major criterion in the Duke criteria for the diagnosis of infective endocarditis...
Corpuscular antigenic microarray for the serodiagnosis of blood culture-negative endocarditisLaurent Samson
, CNRS UMR 6020, IFR 48, , , 27 Bd Jean Moulin, 13385 Marseille Cedex 05, France
Ann N Y Acad Sci 1078:595-6. 2006..Preliminary results indicate that this new serologic test is valuable for the rapid, automated serological diagnosis of blood culture-negative endocarditis...
Legionella drancourtii sp. nov., a strictly intracellular amoebal pathogenBernard La Scola
, CNRS UPRESA 6020, , , 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
Int J Syst Evol Microbiol 54:699-703. 2004..Taken together, these results support the proposal of LLAP12(T) as the type strain of Legionella drancourtii sp. nov...
Bacterial strain typing in the genomic eraWenjun Li
Unité des Rickettsies et pathogènes émergents, CNRS UMR 6236, IRD 198, Faculte de Medecine, Universite de la Mediterranee, Marseille, France
FEMS Microbiol Rev 33:892-916. 2009....
Gene sequence-based criteria for identification of new rickettsia isolates and description of Rickettsia heilongjiangensis sp. novPierre Edouard Fournier
Unite des Rickettsies, IFR 48, CNRS UMR 6020, Faculte de Medecine, Universite de la Mediterranee, 13385 Marseille Cedex 05, France
J Clin Microbiol 41:5456-65. 2003..3% for the ompA and ompB genes and gene D, respectively. By use of our classification scheme, "Rickettsia heilongjiangii" belongs to a new species for which we officially propose the name Rickettsia heilongjiangensis sp. nov...
Streptococcus massiliensis sp. nov., isolated from a patient blood cultureOlga O Glazunova
, , CNRS UMR 6020, IFR48, 264 rue Saint-Pierre, 13385 Marseille, Cedex 05, France
Int J Syst Evol Microbiol 56:1127-31. 2006..ferus and S. sanguinis, respectively, confirming it as belonging to a novel taxon, for which the name Streptococcus massiliensis sp. nov. is proposed. The type strain is 4401825T (=CIP 108498T=CCUG 49690T)...
Bacillus massiliensis sp. nov., isolated from cerebrospinal fluidOlga O Glazunova
, , CNRS UMR 6020, IFR48, 264 rue Saint-Pierre, 13385 Marseille Cedex 05, France
Int J Syst Evol Microbiol 56:1485-8. 2006..The major fatty acid was iso-C(15 : 0) (48 %). The name Bacillus massiliensis sp. nov. is proposed for the novel isolate, with strain 4400831(T) (=CIP 108446(T)=CCUG 49529(T)) as the type strain...
Identification and characterization of a phospholipase D-superfamily gene in rickettsiaePatricia Renesto
Unite des Rickettsies, Centre National de la Recherche Scientifique, Unite Mixte de Recherche 6020, Institut Federatif de Recherche 48, Faculte de Medecine, Marseille, France
J Infect Dis 188:1276-83. 2003..This enzyme might account for the activity previously attributed to PLA(2) and might be critical for the intracellular life of these bacteria...
Q FeverEmmanouil Angelakis
Unite des Rickettsies, CNRS UMR 6020, IFR 48, Faculte de Medecine, Université de Méditerranée, 27 Bd Jean Moulin, 13385 Marseille Cedex 05, France
Vet Microbiol 140:297-309. 2010..Vaccination of animals in infected flocks, as well as in uninfected ones close to them, with an efficient vaccine can prevent abortions and shedding of the bacteria...
Detection of new genotypes of Orientia tsutsugamushi infecting humans in ThailandP E Fournier
Unite des Rickettsies, IFR 48, CNRS UMR6020, Faculte de Medecine, Universite de la Mediterranee, Marseille, France
Clin Microbiol Infect 14:168-73. 2008..Other genotypes formed two other clusters that may also be associated with undescribed serotypes...
Identification of protein candidates for the serodiagnosis of Q fever endocarditis by an immunoproteomic approachZ Sekeyova
Faculte de Medecine, Universite de la Mediterranee, URMITE 6236 IRD, IFR 48, 27 Boulevard Jean Moulin, 05 13385 Marseille Cedex, France
Eur J Clin Microbiol Infect Dis 28:287-95. 2009..Two proteins, arginine repressor and OmpH, were recognised exclusively by the sera of patients with Q fever endocarditis. These proteins are promising candidates for the development of serodiagnostic assays for Q fever endocarditis...
