Tarik Issad

Summary

Country: France

Publications

  1. ncbi Interaction of PTPB with the insulin receptor precursor during its biosynthesis in the endoplasmic reticulum
    T Issad
    Department of Cell Biology, Institut Cochin, CNRS UMR 8104, INSERM U567, Universite Paris V, 22, Rue Mechain, 75014 Paris, France
    Biochimie 87:111-6. 2005
  2. ncbi The use of bioluminescence resonance energy transfer for the study of therapeutic targets: application to tyrosine kinase receptors
    Tarik Issad
    Institut Cochin, Department of Cell Biology, Universite Paris Descartes, CNRS UMR 8104, 22 rue Mechain, 75014 Paris, France
    Expert Opin Ther Targets 11:541-56. 2007
  3. ncbi Looking for an insulin pill? Use the BRET methodology!
    T Issad
    Department of Cell Biology, Institut Cochin, CNRS UMR 8104, INSERM U567, Universite Paris V, Paris, France
    Diabetes Metab 29:111-7. 2003
  4. ncbi The activity of the insulin receptor assessed by bioluminescence resonance energy transfer
    Tarik Issad
    CNRS, INSERM, Department of Cell Biology, Universite Rene Descartes, Cochin Institute, 75014 Paris, France
    Ann N Y Acad Sci 973:120-3. 2002
  5. ncbi A homogenous assay to monitor the activity of the insulin receptor using Bioluminescence Resonance Energy Transfer
    Tarik Issad
    Department of Cell Biology, Cochin Institute, CNRS, INSERM, University Rene Descartes, 22 rue Mechain, 75014 Paris, France
    Biochem Pharmacol 64:813-7. 2002
  6. ncbi Interaction with Grb14 results in site-specific regulation of tyrosine phosphorylation of the insulin receptor
    Sebastien Nouaille
    Departement de Biologie Cellulaire, Institut Cochin, Universite Paris Descartes, 22 rue Mechain, UMRCNRS 8104, INSERM U567, Paris 75014, France
    EMBO Rep 7:512-8. 2006
  7. ncbi Monitoring the activation state of insulin/insulin-like growth factor-1 hybrid receptors using bioluminescence resonance energy transfer
    Christophe Blanquart
    Department of Cell Biology, Institut Cochin, , 75014 Paris, France
    Mol Pharmacol 70:1802-11. 2006
  8. ncbi Monitoring the activation state of the insulin-like growth factor-1 receptor and its interaction with protein tyrosine phosphatase 1B using bioluminescence resonance energy transfer
    Christophe Blanquart
    Department of Cell Biology, Institut Cochin, , 75014 Paris, France
    Mol Pharmacol 68:885-94. 2005
  9. ncbi Protein tyrosine phosphatase-1B and T-cell protein tyrosine phosphatase regulate IGF-2-induced MCF-7 cell migration
    Christophe Blanquart
    Institut Cochin, Universite Paris Descartes, CNRS UMR 8104, Paris, France
    Biochem Biophys Res Commun 392:83-8. 2010
  10. ncbi O-GlcNAc modification of FoxO1 increases its transcriptional activity: a role in the glucotoxicity phenomenon?
    Meishiue Kuo
    Institut Cochin, Universite Paris Descartes, CNRS UMR 8104, Paris, France
    Biochimie 90:679-85. 2008

Collaborators

  • D Lacasa
  • Roger J Daly
  • Lowenna J Holt
  • S Marullo
  • R Jockers
  • Christophe Blanquart
  • Meishiue Kuo
  • Vladimir Zilberfarb
  • Nicolas Boute
  • Nicolas Gangneux
  • N Boute
  • Sebastien Nouaille
  • Salah Eddine Karouri
  • Diana Goenaga
  • V Zilberfarb
  • Nevena Christeff
  • Dominique Perdereau
  • Anne Françoise Burnol
  • Luis G Bermúdez Humarán
  • A D Strosberg
  • Isabelle Broutin
  • Edith Browaeys-Poly
  • Katia Cailliau
  • Cornelia Hampe
  • Nadège Carré
  • Jean Girard
  • C C Gerhardt
  • Josepha Achi
  • Gerard Corthier
  • Philippe Langella
  • Alexandra Gruss
  • Johan Roix
  • Carmen Gonzalez-Yanes
  • S Bonnafous
  • Y Le Marchand-Brustel
  • L Camoin
  • Samira Boubekeur
  • K Siquier
  • K Pernet
  • J Gros

Detail Information

Publications28

  1. ncbi Interaction of PTPB with the insulin receptor precursor during its biosynthesis in the endoplasmic reticulum
    T Issad
    Department of Cell Biology, Institut Cochin, CNRS UMR 8104, INSERM U567, Universite Paris V, 22, Rue Mechain, 75014 Paris, France
    Biochimie 87:111-6. 2005
    ..Localisation of PTP1B to the endoplasmic reticulum may be important to prevent insulin-independent autonomous activity of the immature insulin receptor precursor...
  2. ncbi The use of bioluminescence resonance energy transfer for the study of therapeutic targets: application to tyrosine kinase receptors
    Tarik Issad
    Institut Cochin, Department of Cell Biology, Universite Paris Descartes, CNRS UMR 8104, 22 rue Mechain, 75014 Paris, France
    Expert Opin Ther Targets 11:541-56. 2007
    ..In addition, BRET can be used to develop high-throughput screening assays for the search of molecules with therapeutic interest and could, therefore, constitute a valuable tool for laboratories involved in drug discovery...
  3. ncbi Looking for an insulin pill? Use the BRET methodology!
    T Issad
    Department of Cell Biology, Institut Cochin, CNRS UMR 8104, INSERM U567, Universite Paris V, Paris, France
    Diabetes Metab 29:111-7. 2003
    ..Therefore, this methodology can also be used in high-throughput screening for the search of molecules that will specifically disrupt the interaction between the insulin receptor and PTP1B...
  4. ncbi The activity of the insulin receptor assessed by bioluminescence resonance energy transfer
    Tarik Issad
    CNRS, INSERM, Department of Cell Biology, Universite Rene Descartes, Cochin Institute, 75014 Paris, France
    Ann N Y Acad Sci 973:120-3. 2002
    ..This work describes a new assay, based on the bioluminescence resonance energy transfer (BRET) methodology, to monitor the activation state of the insulin receptor...
  5. ncbi A homogenous assay to monitor the activity of the insulin receptor using Bioluminescence Resonance Energy Transfer
    Tarik Issad
    Department of Cell Biology, Cochin Institute, CNRS, INSERM, University Rene Descartes, 22 rue Mechain, 75014 Paris, France
    Biochem Pharmacol 64:813-7. 2002
    ..This methodology allows for rapid analysis of the effects of agonists on insulin receptor activity and could, therefore, be used in high-throughput screening for the discovery of molecules with insulin-like properties...
  6. ncbi Interaction with Grb14 results in site-specific regulation of tyrosine phosphorylation of the insulin receptor
    Sebastien Nouaille
    Departement de Biologie Cellulaire, Institut Cochin, Universite Paris Descartes, 22 rue Mechain, UMRCNRS 8104, INSERM U567, Paris 75014, France
    EMBO Rep 7:512-8. 2006
    ..Our work therefore suggests that Grb14 may regulate signalling through the IR by controlling its tyrosine dephosphorylation in a site-specific manner...
  7. ncbi Monitoring the activation state of insulin/insulin-like growth factor-1 hybrid receptors using bioluminescence resonance energy transfer
    Christophe Blanquart
    Department of Cell Biology, Institut Cochin, , 75014 Paris, France
    Mol Pharmacol 70:1802-11. 2006
    ..Therefore, both methods allow monitoring of the activity of IRA/IGF1R hybrid receptor and could be used to detect molecules of therapeutic interest for the treatment of cancer...
  8. ncbi Monitoring the activation state of the insulin-like growth factor-1 receptor and its interaction with protein tyrosine phosphatase 1B using bioluminescence resonance energy transfer
    Christophe Blanquart
    Department of Cell Biology, Institut Cochin, , 75014 Paris, France
    Mol Pharmacol 68:885-94. 2005
    ..In conclusion, these BRET-based assays permit 1) the rapid evaluation of the effects of agonists or inhibitory molecules on IGF1R activation and 2) the analysis of the regulation of IGF1R-PTP1B interaction in living cells...
  9. ncbi Protein tyrosine phosphatase-1B and T-cell protein tyrosine phosphatase regulate IGF-2-induced MCF-7 cell migration
    Christophe Blanquart
    Institut Cochin, Universite Paris Descartes, CNRS UMR 8104, Paris, France
    Biochem Biophys Res Commun 392:83-8. 2010
    ..This novel finding reinforces the potential role of these PTPases as therapeutic targets for treatment of breast cancer...
  10. ncbi O-GlcNAc modification of FoxO1 increases its transcriptional activity: a role in the glucotoxicity phenomenon?
    Meishiue Kuo
    Institut Cochin, Universite Paris Descartes, CNRS UMR 8104, Paris, France
    Biochimie 90:679-85. 2008
    ....
  11. ncbi Characterization of IRA/IRB hybrid insulin receptors using bioluminescence resonance energy transfer
    Christophe Blanquart
    Institut Cochin, Universite Paris Descartes, CNRS UMR 8104, Paris, France
    Biochem Pharmacol 76:873-83. 2008
    ....
  12. ncbi Dynamics of the interaction between the insulin receptor and protein tyrosine-phosphatase 1B in living cells
    Nicolas Boute
    Department of Cell Biology, Institut Cochin, CNRS UMR 8104, INSERM U567, Universite Paris V, 22 rue Mechain, 75014 Paris, France
    EMBO Rep 4:313-9. 2003
    ..Therefore, localization of PTP1B in this compartment might be important for the regulation of insulin receptors during their biosynthesis...
  13. ncbi [A new mode of reglulation of FoxO1 by O-GlcNAc glycosylation: involvement in the glucotoxicity phenomenon]
    Meishiue Kuo
    Institut Cochin, , CNRS (UMR 8104, INSERM, U567, 22, , 75014 Paris, France
    Med Sci (Paris) 24:369-71. 2008
  14. ncbi [O-GlcNAc glycosylation and regulation of cell signaling]
    Tarik Issad
    Institut Cochin, 22, 75014 Paris, France
    Med Sci (Paris) 26:753-9. 2010
    ..This negative feedback may be exacerbated when cells are exposed to elevated glucose concentrations as observed in diabetic patients, and could thereby contribute to insulin resistance and worsening of hyperglycaemia. double dagger...
  15. ncbi Bioluminescence resonance energy transfer to monitor protein-protein interactions
    Tarik Issad
    Institut Cochin, INSERM, CNRS, , France
    Methods Mol Biol 332:195-209. 2006
    ..Determination of the proportion of the dimeric vs monomeric form of a protein in the cell also is possible. Therefore, the BRET technology can be considered as a new and powerful tool in the field of protein-protein interactions...
  16. ncbi Molecular determinants of Grb14-mediated inhibition of insulin signaling
    Diana Goenaga
    Institut Cochin, Universite Paris Descartes, Centre National de la Recherche Scientifique CNRS Unité Mixte de Recherche UMR 8104, Paris, France
    Mol Endocrinol 23:1043-51. 2009
    ..Indeed, a precise knowledge of the molecular mechanism of insulin signaling inhibition by Grb14 is a prerequisite for the development of insulin-sensitizing molecules to treat pathophysiological states such as obesity or type 2 diabetes...
  17. ncbi Desensitization of the beta-adrenergic response in human brown adipocytes
    R Jockers
    CNRS UPR 0415 and Université Paris VII, Institut Cochin de Genetique Moleculaire, France
    Endocrinology 139:2676-84. 1998
    ..Further studies should establish whether the beta3AR is coupled to other signaling pathways in human brown adipocytes and whether these may contribute to BAT hypertrophy and/or thermogenesis...
  18. ncbi O-glycosylation of FoxO1 increases its transcriptional activity towards the glucose 6-phosphatase gene
    Meishiue Kuo
    Institut Cochin, Universite Paris Descartes, 22 rue Mechain, CNRS UMR 8104, Paris, France
    FEBS Lett 582:829-34. 2008
    ..Since glucose-6-phosphatase plays a key role in hepatic glucose production, our observation may be of importance with regard to glucotoxicity associated with chronic hyperglycaemia in diabetes...
  19. ncbi The use of resonance energy transfer in high-throughput screening: BRET versus FRET
    Nicolas Boute
    Institut Cochin, CNRS UMR 8104, INSERM U567, , , , 75014, Paris, France
    Trends Pharmacol Sci 23:351-4. 2002
    ..This technique constitutes an important tool for the study of the functional activity of different types of receptors, and can be used in sensitive, homogenous high-throughput screening assays...
  20. ncbi O-GlcNAc modification of transcription factors, glucose sensing and glucotoxicity
    Tarik Issad
    Institut Cochin, Universite Paris Descartes, CNRS UMR 8104, Paris, France
    Trends Endocrinol Metab 19:380-9. 2008
    ....
  21. ncbi Monitoring the activation state of the insulin receptor using bioluminescence resonance energy transfer
    N Boute
    , , Paris, France
    Mol Pharmacol 60:640-5. 2001
    ..This method allows for rapid analysis of the effects of agonists on insulin receptor activity and could therefore be used in a high-throughput screening test for discovery of molecules with insulin-like properties...
  22. ncbi Implication of protein tyrosine phosphatase 1B in MCF-7 cell proliferation and resistance to 4-OH tamoxifen
    Christophe Blanquart
    Institut Cochin, Universite Paris Descartes, CNRS, UMR, Paris, France
    Biochem Biophys Res Commun 387:748-53. 2009
    ..siRNA experiments showed that PTP1B, but not TC-PTP, is necessary for resistance to 4-OH tamoxifen. Therefore, our work indicates that PTP1B could be a relevant therapeutic target for treatment of tamoxifen-resistant breast cancers...
  23. ncbi Stimulation of the extracellular signal-regulated kinase 1/2 pathway by human beta-3 adrenergic receptor: new pharmacological profile and mechanism of activation
    C C Gerhardt
    Institut Cochin de Genetique Moleculaire, Centre National de la Recherche Scientifique and Université Paris VII, Laboratoire d Immuno Pharmacologie Moléculaire, Paris, France
    Mol Pharmacol 55:255-62. 1999
    ..As was observed for the activation of ERK, the activation of protein kinase B is inhibited by preincubation with pertussis toxin and PI3K inhibitors, suggesting that both are a consequence of a Gi/o-mediated activation of PI3K...
  24. ncbi Effect of dexamethasone on adipocyte differentiation markers and tumour necrosis factor-alpha expression in human PAZ6 cells
    V Zilberfarb
    Institute Cochin of Molecular Genetics, Paris, France
    Diabetologia 44:377-86. 2001
    ....
  25. ncbi The formation of an intrachain disulfide bond in the leptin protein is necessary for efficient leptin secretion
    N Boute
    Department of Cell Biology, Institut Cochin, UMR CNRS 8104, INSERM U567, Universite Paris 5, 22, Rue Mechain, 75014 Paris, France
    Biochimie 86:351-6. 2004
    ..Moreover, the similarity of the behavior of R105W mutant and cystein mutants suggests that the lack of secretion observed with the naturally occurring mutant could result from impaired disulfide bond formation...
  26. ncbi Effects of intranasal administration of a leptin-secreting Lactococcus lactis recombinant on food intake, body weight, and immune response of mice
    Luis G Bermúdez Humarán
    Unité d Ecologie et de Physiologie du Système Digestif, INRA, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France
    Appl Environ Microbiol 73:5300-7. 2007
    ..These results demonstrate that leptin can be produced and secreted in an active form by L. lactis and that leptin-producing L. lactis regulates in vivo antigen-specific immune responses, as well as body weight and food consumption...