Research Topics
Genomes and GenesSpecies | Gilles CurienSummaryCountry: France Publications
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Publications
Identification of six novel allosteric effectors of Arabidopsis thaliana aspartate kinase-homoserine dehydrogenase isoforms. Physiological context sets the specificityGilles Curien
Laboratoire de Physiologie Cellulaire Végétale UMR5168, CEA CNRS INRA Université Joseph Fourier, 38054 Grenoble, France
J Biol Chem 280:41178-83. 2005..Thus, physiological context rather than enzyme structure sets the specificity of the allosteric control. Stimulation by alanine may play the role of a feed forward activation of the aspartate-derived amino acid pathway in plant...
Allosteric monofunctional aspartate kinases from ArabidopsisGilles Curien
Laboratoire de Physiologie cellulaire Végétale PCV DRDC, CEA CNRS INRA Université Joseph Fourier, Grenoble, France
FEBS J 274:164-76. 2007..The increase in aspartate kinase 1 apparent affinity for lysine in the presence of SAM thus results from the displacement of the unfavorable equilibrium between aspartate kinase 1 and aspartate kinase 1-Lys towards the inactive form...
Understanding the regulation of aspartate metabolism using a model based on measured kinetic parametersGilles Curien
CNRS, UMR 5168, 17 rue des Martyrs, Grenoble, France
Mol Syst Biol 5:271. 2009..Another result is the identification of the threonine concentration as the most sensitive variable in the system, suggesting a regulatory role for threonine at a higher level of integration...
Methionine metabolism in plants: chloroplasts are autonomous for de novo methionine synthesis and can import S-adenosylmethionine from the cytosolStéphane Ravanel
Departement de Reponse et Dynamique Cellulaires, CEA Grenoble, 17 rue des Martyrs, 38054 Grenoble Cedex 9, France
J Biol Chem 279:22548-57. 2004..Therefore, the chloroplastic AdoMet carrier serves as a link between cytosolic and chloroplastic one-carbon metabolism...
A new mode of dimerization of allosteric enzymes with ACT domains revealed by the crystal structure of the aspartate kinase from CyanobacteriaAdeline Y Robin
Centre National de la Recherche Scientifique, Unité Mixte de Recherche 5168, Grenoble, France
J Mol Biol 399:283-93. 2010....
Amino acid biosynthesis: new architectures in allosteric enzymesGilles Curien
Laboratoire de Physiologie Cellulaire Végétale, Universite Joseph Fourier, Commissariat a l Energie Atomique, Institut de Recherche et de Technologie des Sciences du Vivant, 38054 Grenoble, France
Plant Physiol Biochem 46:325-39. 2008....
Regulation of one-carbon metabolism in Arabidopsis: the N-terminal regulatory domain of cystathionine gamma-synthase is cleaved in response to folate starvationKaren Loizeau
Laboratoire de Physiologie Cellulaire Végétale, Institut National de la Recherche Agronomique, Universite Joseph Fourier Grenoble I, Grenoble, France
Plant Physiol 145:491-503. 2007..We suggest that a metabolic effector, to date unidentified, can modulate CGS activity in vivo through an interaction with the N-terminal domain of the enzyme and that removal of this domain can suppress this regulation...
Identification of a plant gene encoding glutamate/aspartate-prephenate aminotransferase: the last homeless enzyme of aromatic amino acids biosynthesisMatthieu Graindorge
Université Grenoble 1, Grenoble, France
FEBS Lett 584:4357-60. 2010..Our data revealed that this activity is housed by the prokaryotic-type plastidic aspartate aminotransferase (At2g22250). This represents the first identification of a gene encoding PAT...
A kinetic model of the branch-point between the methionine and threonine biosynthesis pathways in Arabidopsis thalianaGilles Curien
Laboratoire de Physiologie Cellulaire Végétale DRDC CEA Grenoble, France
Eur J Biochem 270:4615-27. 2003..Threonine synthase and cystathionine gamma-synthase display similar kinetic efficiencies in the metabolic context considered and are first-order for the phosphohomoserine substrate. Under these conditions outflows are coordinated...
A novel organization of ACT domains in allosteric enzymes revealed by the crystal structure of Arabidopsis aspartate kinaseCorine Mas-Droux
Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Universite Joseph Fourier, Commissariat a l Energie Atomique, Departement Reponse et Dynamique Cellulaires, France
Plant Cell 18:1681-92. 2006..The organization of ACT domains in the present structure is different from that observed in Thr deaminase and in the regulatory subunit of acetohydroxyacid synthase III...
Mechanism of control of Arabidopsis thaliana aspartate kinase-homoserine dehydrogenase by threonineStephane Paris
Unité Mixte de Recherche 1932, Laboratoire Mixte CNRS Institut National de la Recherche Agronomique Bayer Cropscience, 14 20 rue Pierre Baizet, 69263 Lyon, France
J Biol Chem 278:5361-6. 2003..Interaction of this second threonine with Gln(524) leads to inhibition of homoserine dehydrogenase activity...
