Research Topics
Genomes and Genes | Jean Michel ClaverieSummaryAffiliation: Centre National de la Recherche Scientifique Country: France Publications
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Publications
Mimivirus relatives in the Sargasso seaElodie Ghedin
Department of Parasite and Virus Genomics, The Institute for Genomic Research, Rockville, MD 20850, USA
Virol J 2:62. 2005..Their isolation and genome sequencing could prove invaluable in understanding the origin and diversity of large DNA viruses, and shed some light on the role they eventually played in the emergence of eukaryotes...
Determination of strongly overlapping signaling activity from microarray dataGhislain Bidaut
Fox Chase Cancer Center, 333 Cottman Avenue, Philadelphia, PA 19111, USA
BMC Bioinformatics 7:99. 2006....
Fewer genes, more noncoding RNAJean Michel Claverie
Structural and Genomics Information Laboratory, CNRS UPR 2589, Institut de Biologie Structurale et Microbiologie, 31 Chemin Joseph Aiguier, Marseille 13402, France
Science 309:1529-30. 2005..Exploring the role and diversity of these numerous noncoding RNAs now constitutes a main challenge in transcription research...
Recent advances in computational genomicsJ M Claverie
Structural and Genetic Information Laboratory, UMR 1889 CNRS AVENTIS, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
Pharmacogenomics 2:361-72. 2001..It is thus increasingly difficult for the biologist to choose the computational approaches that perform best in these various areas. This paper attempts to review the most useful developments of the last 2 years...
Mimivirus and the emerging concept of "giant" virusJean Michel Claverie
Information Genomique et Structurale, CNRS UPR 2589, IBSM, Parc Scientifique de Luminy, 163 Avenue de Luminy, Case 934, 13288 Marseille Cedex 9, France
Virus Res 117:133-44. 2006..We propose to introduce the word "girus" to recognize the intermediate status of these giant DNA viruses, the genome complexity of which makes them closer to small parasitic prokaryotes than to regular viruses...
Giant viruses in the oceans: the 4th Algal Virus WorkshopJean Michel Claverie
Structural and Genomic Information Laboratory, UPR 2589, IBSM, CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
Virol J 2:52. 2005....
Viruses take center stage in cellular evolutionJean Michel Claverie
Structural and Genomic Information Laboratory, CNRS UPR2589, IBSM, Parc Scientifique de Luminy, 163 Avenue de Luminy, Case 934, Marseille 13288, Cedex 9, France
Genome Biol 7:110. 2006....
The insertion of palindromic repeats in the evolution of proteinsJean Michel Claverie
Information Genetique et Structurale, CNRS AVENTIS UMR 1889, Institut de Biologie Structurale et Microbiologie, Marseille, France
Trends Biochem Sci 28:75-80. 2003..Based on previous results and theories on the coding potential of palindromic elements, we speculate that their insertion and mobility might have played a significant role in the early stages of protein evolution...
Mimivirus and its virophageJean Michel Claverie
Structural and Genomic Information Laboratory, CNRS UPR 2589, IFR 88, Aix Marseille University, Parc Scientifique de Luminy, Case 934, FR 13288 Marseille, France
Annu Rev Genet 43:49-66. 2009..Post-genomic studies are now in progress, slowly shedding some light on the physiology of the most complex virus isolated to date...
Mimivirus and Mimiviridae: giant viruses with an increasing number of potential hosts, including corals and spongesJean Michel Claverie
Structural and Genomic Information Laboratory, CNRS UPR 2589, IFR 88, Parc Scientifique de Luminy, Case 934, Marseille, France
J Invertebr Pathol 101:172-80. 2009....
The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasiteHiroyuki Ogata
Structural and Genomic Information Laboratory, UPR 2589, IBSM, CNRS, Marseille Cedex, France
PLoS Biol 3:e248. 2005..Our study demonstrates that complete genome sequencing is the fastest approach to reveal phenotypic characters of recently cultured obligate intracellular bacteria...
Phydbac (phylogenomic display of bacterial genes): An interactive resource for the annotation of bacterial genomesFrançois Enault
Structural and Genomic Information CNRS UPR2589, Institut de Biologie Structurale et Microbiologie, 31 Chemin Joseph Aiguier, 13402 Marseille, Cedex 20, France
Nucleic Acids Res 31:3720-2. 2003..An extension of Phydbac, including precomputed profiles for all available bacterial genomes (including major pathogens) will soon be available. Phydbac can be accessed at: http://igs-server.cnrs-mrs.fr/phydbac/...
Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogensHiroyuki Ogata
Structural and Genomic Information Laboratory, Centre National de la Recherche Scientifique UPR 2589, Institut de Biologie Structurale et Microbiologie, Parc Scientifique de Luminy, Marseille, France
PLoS Genet 2:e76. 2006....
Structural genomics of highly conserved microbial genes of unknown function in search of new antibacterial targetsChantal Abergel
Structural and Genomic Information Laboratory, UMR 1889 CNRS AVENTIS, 13402 Marseille Cedex 20, France
J Struct Funct Genomics 4:141-57. 2003....
Reductive genome evolution from the mother of RickettsiaGuillaume Blanc
Structural and Genomic Information Laboratory, Institut de Biologie Structurale et Microbiologie, Parc Scientifique de Luminy, Marseille, France
PLoS Genet 3:e14. 2007....
Impact of the excision of an ancient repeat insertion on Rickettsia conorii guanylate kinase activityChantal Abergel
Information Génomique and Structurale, CNRS UPR 2589, IBSM, Marseille Cedex, France
Mol Biol Evol 23:2112-22. 2006....
Molecular evolution of rickettsia surface antigens: evidence of positive selectionGuillaume Blanc
Information Genomique et Structurale, UPR 2589, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
Mol Biol Evol 22:2073-83. 2005....
The conserved Candida albicans CA3427 gene product defines a new family of proteins exhibiting the generic periplasmic binding protein structural foldSébastien Santini
Information Génomique et Structurale CNRS UPR2589, Aix Marseille Universite, Mediterranean Institute of Microbiology, Parc Scientifique de Luminy, Marseille, France
PLoS ONE 6:e18528. 2011..It was then lost by the Saccharomycetales which include Saccharomyces cerevisiae...
mRNA deep sequencing reveals 75 new genes and a complex transcriptional landscape in MimivirusMatthieu Legendre
Structural and Genomic Information Laboratory, Centre National de la Recherche Scientifique, UPR2589, Mediterranean Institute of Microbiology IFR88, Aix Marseille University, Parc Scientifique de Luminy, FR 13288 Marseille, France
Genome Res 20:664-74. 2010....
Protein coding palindromes are a unique but recurrent feature in RickettsiaHiroyuki Ogata
Information Génétique and Structurale, CNRS AVENTIS UMR 1889, 13402 Marseille Cedex 20, France
Genome Res 12:808-16. 2002..This comprehensive study of Rickettsia repeats confirms and extends our previous observations and further indicates a significant role of selfish DNAs in the creation and modification of proteins...
Mimivirus TyrRS: preliminary structural and functional characterization of the first amino-acyl tRNA synthetase found in a virusChantal Abergel
Structural and Genomic Information Laboratory, CNRS UPR 2589, IBSM, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
Acta Crystallogr Sect F Struct Biol Cryst Commun 61:212-5. 2005..Experimental evidence that Mimivirus tyrosyl tRNA synthetase recombinant gene product does indeed activate tyrosine is also presented...
Phydbac2: improved inference of gene function using interactive phylogenomic profiling and chromosomal location analysisFrançois Enault
Information Génomique and Structurale UPR CNRS 2589, Institut de Biologie Structurale et Microbiologie, 31, Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
Nucleic Acids Res 32:W336-9. 2004..The Phydbac2 web server is available at http://igs-server.cnrs-mrs.fr/phydbac/...
Phydbac "Gene Function Predictor": a gene annotation tool based on genomic context analysisFrançois Enault
Structural and Genomic Information, CNRS UPR 2589, 31 Chemin Joseph Aiguier, 13009 Marseille, France
BMC Bioinformatics 6:247. 2005....
Mimivirus gene promoters exhibit an unprecedented conservation among all eukaryotesKarsten Suhre
Information Genomique et Structurale, Centre National de la Recherche Scientifique, Institut de Biologie Structurale et Microbiologie, 13402 Marseille, France
Proc Natl Acad Sci U S A 102:14689-93. 2005..This unprecedented conservation of core promoter regions is another exceptional feature of Mimivirus that again raises the question of its evolutionary origin...
Comparative genomics of multidrug resistance in Acinetobacter baumanniiPierre Edouard Fournier
Information Genomique et Structurale, Institute for Structural Biology and Microbiology, IBSM, Marseille, France
PLoS Genet 2:e7. 2006..Whole-genome sequencing appears to be a fast and efficient approach to the exhaustive identification of resistance genes in epidemic infectious agents of clinical significance...
FusionDB: a database for in-depth analysis of prokaryotic gene fusion eventsKarsten Suhre
Information Génomique and Structurale, CNRS UPR 2589, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
Nucleic Acids Res 32:D273-6. 2004..Together, these servers provide the complete set of information required for in-depth analysis of non-homology-based gene function attribution...
BLAST-EXPLORER helps you building datasets for phylogenetic analysisAlexis Dereeper
Information Génomique and Structurale CNRS UPR2589, Institut de Microbiologie de la Méditerranée IFR 88, Marseille, France
BMC Evol Biol 10:8. 2010....
Lateral gene transfer between obligate intracellular bacteria: evidence from the Rickettsia massiliae genomeGuillaume Blanc
Structural and Genomic Information Laboratory, CNRS UPR 2589, Institut de Biologie Structurale et Microbiologie, IFR 88, Parc Scientifique de Luminy, 13288 Marseille Cedex 9, France
Genome Res 17:1657-64. 2007..Further analysis of the genomic sequences identifies additional candidates of LGT between Rickettsia. Our study indicates that recent LGT between obligate intracellular Rickettsia is more common than previously thought...
Marine mimivirus relatives are probably large algal virusesAdam Monier
Structural and Genomic Information Laboratory, CNRS UPR 2589, IBSM, Parc Scientifique de Luminy, 163 Avenue de Luminy, Case 934, 13288 Marseille Cedex 9, France
Virol J 5:12. 2008..The existence of mimivirus relatives was first suggested by the analysis of the Sargasso Sea metagenomic data...
Horizontal gene transfer and nucleotide compositional anomaly in large DNA virusesAdam Monier
Structural and Genomic Information Laboratory, CNRS UPR 2589, Institute for Structural Biology and Microbiology, Parc Scientifique de Luminy, 163 Avenue de Luminy, FR 13288, Marseille Cedex 09, France
BMC Genomics 8:456. 2007..We then interpreted their compositional heterogeneity in terms of possible causes, including strand asymmetry, gene function/expression, and horizontal transfer...
A new example of viral intein in MimivirusHiroyuki Ogata
Information Genomique et Structurale, UPR2589 CNRS, IBSM, IFR88, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
Virol J 2:8. 2005..After their discovery, inteins have been quickly identified in all domains of life, but only once to date in the genome of a eukaryote-infecting virus...
The polyadenylation site of Mimivirus transcripts obeys a stringent 'hairpin rule'Deborah Byrne
Structural and Genomic Information Laboratory, CNRS UPR 2589, IFR 88, Aix Marseille University, Parc Scientifique de Luminy, Case 934, 13288 Marseille Cedex 9, France
Genome Res 19:1233-42. 2009..The precise molecular mechanisms implementing the hairpin rule into the 3'-end processing of Mimivirus pre-mRNAs remain to be elucidated...
Distant Mimivirus relative with a larger genome highlights the fundamental features of MegaviridaeDefne Arslan
Information Genomique et Structurale, Centre National de la Recherche Scientifique Unité Propre de Recherche 2589, Aix Marseille University, Institut de Microbiologie de la Mediterranee, Parc Scientifique de Luminy, Case 934, FR 13288 Marseille, France
Proc Natl Acad Sci U S A 108:17486-91. 2011....
Two new subfamilies of DNA mismatch repair proteins (MutS) specifically abundant in the marine environmentHiroyuki Ogata
Information Genomique et Structurale, CNRS UPR2589, Institut de Microbiologie de la Mediterranee, Parc Scientifique de Luminy, Aix Marseille Universite, Marseille Cedex 9, France
ISME J 5:1143-51. 2011..Giruses thus seem to represent a major source of the underexplored diversity of the MutS family in the microbial world...
Taxonomic distribution of large DNA viruses in the seaAdam Monier
Structural and Genomic Information Laboratory, CNRS UPR 2589, IFR 88, Université de la Méditerranée Parc Scientifique de Luminy, Avenue de Luminy, FR 13288 Marseille, France
Genome Biol 9:R106. 2008..In this study, we used a new approach--'phylogenetic mapping'--to obtain a comprehensive picture of the taxonomic distribution of large DNA viruses represented in the Sorcerer II Global Ocean Sampling Expedition metagenomic data set...
Virus-encoded aminoacyl-tRNA synthetases: structural and functional characterization of mimivirus TyrRS and MetRSChantal Abergel
Structural and Genomic Information Laboratory, CNRS UPR2589, IBSM IFR88, 163 Avenue de Luminy, Case 934, 13288, Marseille Cedex 9, France
J Virol 81:12406-17. 2007....
Genome analysis of Minibacterium massiliensis highlights the convergent evolution of water-living bacteriaStephane Audic
Structural and Genomic Information Laboratory, Institute for Structural Biology and Microbiology, Marseille, France
PLoS Genet 3:e138. 2007..The M. massiliensis genome analyses strongly suggest that water-living bacteria are a common source for genes involved in heavy-metal resistance, antibiotics resistance, and virulence factors...
Structure and evolution of the Ivy protein family, unexpected lysozyme inhibitors in Gram-negative bacteriaChantal Abergel
Structural and Genomic Information Laboratory, Centre National de la Recherche Scientifique, Unité Propre de Recherche 2589, 13288 Marseille, Cedex 9, France
Proc Natl Acad Sci U S A 104:6394-9. 2007..As such, Ivy acts as a virulence factor for a number of Gram-negative bacteria-infecting vertebrates...
Mechanisms of evolution in Rickettsia conorii and R. prowazekiiH Ogata
, CNRS-AVENTIS UMR 1889, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
Science 293:2093-8. 2001..A 38-kilobase sequence inversion was dated shortly after the divergence of the genus...
Metagrowth: a new resource for the building of metabolic hypotheses in microbiologyHiroyuki Ogata
Information Genomique et Structurale, CNRS UPR 2589, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
Nucleic Acids Res 33:D321-4. 2005..The web interface of Metagrowth helps users to design new axenic culture media eventually suitable for those bacteria. Metagrowth is accessible at http://igs-server.cnrs-mrs.fr/axenic/...
Breaking the 1000-gene barrier for Mimivirus using ultra-deep genome and transcriptome sequencingMatthieu Legendre
Structural and genomic Information Laboratory CNRS, UPR2589, Mediterranean Institute of Microbiology, Aix Marseille Universite, 163 Avenue de Luminy, Case 934, FR 13288 Marseille, France
Virol J 8:99. 2011..Its 1.2 Mb-genome was initially predicted to encode 917 genes. A subsequent RNA-Seq analysis precisely mapped many transcript boundaries and identified 75 new genes...
Dissecting the unique nucleotide specificity of mimivirus nucleoside diphosphate kinaseSandra Jeudy
Structural and Genomic Information Laboratory, CNRS UPR2589, IFR88, Marseille Cedex 9, France
J Virol 83:7142-50. 2009..Our results suggest that the mimivirus enzyme progressively evolved from an ancestral NDK under the constraints of optimizing its efficiency for the replication of an AT-rich (73%) viral genome in a thymidine-limited host environment...
The genome sequence of Brucella pinnipedialis B2/94 sheds light on the evolutionary history of the genus BrucellaStephane Audic
Laboratoire Information Génomique et Structurale, CNRS UPR2589, Aix Marseille University, Institut de Microbiologie de la Mediterranee, IFR 88, Parc Scientifique de Luminy 163 Avenue de Luminy Case 934 FR 13288, Marseille Cedex 09, France
BMC Evol Biol 11:200. 2011..pinnipedialis and B. ceti from marine mammals raised many questions about their origin as well as on the evolutionary history of the whole genus...
Horizontal gene transfer of an entire metabolic pathway between a eukaryotic alga and its DNA virusAdam Monier
Structural and Genomic Information Laboratory, CNRS UPR2589, Mediterranean Institute of Microbiology IFR 88, Universite de la Mediterranee, Parc Scientifique de Luminy, Marseille, France
Genome Res 19:1441-9. 2009....
Tentative mapping of transcription-induced interchromosomal interaction using chimeric EST and mRNA dataPer Unneberg
Structural and Genomic Information Laboratory, Centre National de la Recherche Scientifique CNRS UPR 2589, Institut de Biologie Structurale et Microbiologie, Marseille, France
PLoS ONE 2:e254. 2007..Due to their higher quality, the mRNA findings are of particular interest and we hope that they may serve as food for thought for specialists in diverse areas of molecular biology...
Reverse transcriptase-polymerase chain reaction validation of 25 "orphan" genes from Escherichia coli K-12 MG1655J P Alimi
Structural and Genetic Information Laboratory, Marseille, France
Genome Res 10:959-66. 2000..By extension, this implies that randomly occurring "junk" ORFs have been actively counter selected during the evolution of the dense E. coli genome...
Brucella microti: the genome sequence of an emerging pathogenStephane Audic
Laboratoire Information Génomique et Structurale, CNRS UPR2589, Aix Marseille University, Institut de Microbiologie de la Méditerranée IM2, IFR 88, Parc Scientifique de Luminy, FR 13288, Marseille Cedex 09, France
BMC Genomics 10:352. 2009..The atypical phenotype of B. microti prompted us to look for genomic differences compared to other Brucella species and to look for similarities with Ochrobactrum...
Mimivirus: the emerging paradox of quasi-autonomous virusesJean Michel Claverie
Structural and Genomic Information Laboratory, CNRS UPR 2589, Aix Marseille University, Mediterranean Institute of Microbiology, Parc Scientifique de Luminy, Case 934, 13288 Marseille Cedex 9, France
Trends Genet 26:431-7. 2010....
The genome of Borrelia recurrentis, the agent of deadly louse-borne relapsing fever, is a degraded subset of tick-borne Borrelia duttoniiMagali Lescot
Structural and Genomic Information Laboratory, CNRS UPR2589, IFR88, Parc Scientifique de Luminy, Marseille, France
PLoS Genet 4:e1000185. 2008..The correlation between gene loss and increased virulence of B. recurrentis parallels that of Rickettsia prowazekii, with both species being genomic subsets of less-virulent strains...
The structure of Acidithiobacillus ferrooxidans c(4)-cytochrome: a model for complex-induced electron transfer tuningChantal Abergel
Information Genomique et Structurale, UPR 2589, Institut de Biologie Structurale et Microbiologie, 31 Chemin Joseph Aiguier, 13402 20, Marseille Cedex, France
Structure 11:547-55. 2003....
The Chlorella variabilis NC64A genome reveals adaptation to photosymbiosis, coevolution with viruses, and cryptic sexGuillaume Blanc
Centre National de la Recherche Scientifique, Laboratoire Information Génomique et Structurale UPR2589, Aix Marseille Universite, Institut de Microbiologie de la Mediterranee, 13009 Marseille, France
Plant Cell 22:2943-55. 2010..Analysis of the NC64A genome substantially advances our understanding of the green lineage evolution, including the genomic interplay with viruses and symbiosis between eukaryotes...
Unique genes in giant viruses: regular substitution pattern and anomalously short sizeHiroyuki Ogata
Structural and Genomic Information Laboratory CNRS UPR 2589, IBSM Parc Scientifique de Luminy, Case 934 13288 Marseille Cedex 9, France
Genome Res 17:1353-61. 2007..We propose that their characteristic features are the consequences of the specific evolutionary forces shaping the viral gene repertoires in the context of their parasitic lifestyles...
Annotation of bacterial genomes using improved phylogenomic profilesF Enault
Structural and Genomic Information, CNRS UPR 2589, 31 Chemin Joseph Aiguier, 13009 Marseille, France
Bioinformatics 19:i105-7. 2003..In contrast to earlier work on the same topic, our approach avoids the use of arbitrary cut-off values, and provides a reliability estimate of the functional predictions in form of p-values...
Comparative analysis of the Arabidopsis and rice expressed sequence tag (EST) setsR Ewing
Carnegie Institution of Washington, 260 Panama Street, Stanford, CA 94305, USA
In Silico Biol 1:197-213. 1999..Our results show that 58% of non-singleton ESTs are derived from genes in gene families common to the two species. These gene families constitute the basis of a core set of higher plant genes...
Self-identification of protein-coding regions in microbial genomesS Audic
Structural and Genetic Information Laboratory, Centre National de la Recherche Scientifique EP 91, 31 rue Joseph Aiguier, Marseille F 13402, France
Proc Natl Acad Sci U S A 95:10026-31. 1998..The results show that protein-coding regions can be identified with an accuracy of up to 90% with a totally automated and objective procedure...
Phylogeny.fr: robust phylogenetic analysis for the non-specialistA Dereeper
Information Génomique et Structurale IGS, CNRS UPR2589, IFR 88, Marseille, France
Nucleic Acids Res 36:W465-9. 2008..A guide tree then helps to select neighbor sequences to be used as input for the phylogeny pipeline. Phylogeny.fr is available at: http://www.phylogeny.fr/..
Alternate polyadenylation in human mRNAs: a large-scale analysis by EST clusteringD Gautheret
Structural and Genetic Information, Center National de la Recherche Scientifique E P 91, 13 402 Marseille Cedex 20, France
Genome Res 8:524-30. 1998..This study demonstrates that, besides quantitative aspects of gene expression, the distribution of alternate mRNA forms can be analyzed through EST sampling...
Structure of the Escherichia coli TolB protein determined by MAD methods at 1.95 A resolutionC Abergel
Information Genetique et Structurale, CNRS UMR 1889 Institut de Biologie Structurale et Microbiologie 31 Chemin Joseph Aiguier, Marseille, 13402, Cedex 20, France
Structure 7:1291-300. 1999..TolB homologues are found in many gram-negative bacteria and the Tol-PAL system is thought to play a role in bacterial envelope integrity. TolB is required for lethal infection by Salmonella typhimurium in mice...
Computational methods for the identification of genes in vertebrate genomic sequencesJ M Claverie
Structural and Genetic Information Laboratory, CNRS EP 91, Marseille, France
Hum Mol Genet 6:1735-44. 1997..As the human and mouse genome sequencing projects enter a production mode, the fully automated annotation of megabase-long anonymous genomic sequences is the next big challenge in bioinformatics...
The difficulty of identifying genes in anonymous vertebrate sequencesJ M Claverie
Structural and Genetic Information Laboratory, C N R S E P 91, Institute of Structural Biology and Microbiology, Marseille, France
Comput Chem 21:203-14. 1997..Thus, it appears that our only hope of turning genome data into genome information must rely on drastic progresses in the way we identify and analyse genes in silico...
Crystallization and preliminary crystallographic study of a component of the Escherichia coli tol system: TolBC Abergel
Information Genetique et Structurale, EP 91, Institut de Biologie Structurale et Microbiologie, 31 Chemin Joseph Aiguier, Marseille, 13402 cedex 20, France
Acta Crystallogr D Biol Crystallogr 54:102-4. 1998..In addition, a selenomethionine-substituted protein is being produced in order to use the MAD method for structure determination...
The statistical significance of nucleotide position-weight matrix matchesJ M Claverie
Structural and Genetic Information Laboratory, CNRS E P 91, Institute of Structural Biology and Microbiology, Marseille, France
Comput Appl Biosci 12:431-9. 1996..An original feature of NMksite is the numerical computation of the statistical significance of PWM matches. The utility of this concept is demonstrated in the context of the prediction of splice sites and promoter regions...
Computational methods for exon detectionJ M Claverie
Mol Biotechnol 10:27-48. 1998..After reviewing the intrinsic difficulties in interpreting vertebrate genomic sequences, this article presents the state-of-the-art, with an emphasis on similarity search methods and the resources available through Internet...
Large-scale statistical analyses of rice ESTs reveal correlated patterns of gene expressionR M Ewing
Structural and Genetic Information SGI Laboratory, Centre National de la Recherche Scientifique Unité mixte de Recherche 1889, Marseille 13402 cedex 20, France
Genome Res 9:950-9. 1999..The analysis presented here combines the application of a correlation-based clustering method with a graphical color map allowing intuitive visualization of patterns within a large table of expression measurements...
Escherichia coli ykfE ORFan gene encodes a potent inhibitor of C-type lysozymeV Monchois
Information Genetique et Structurale, UMR1889 CNRS AVENTIS and Laboratoire d Ingénierie des Systèmes Macromoléculaires, UPR 9027, 31 Chemin Joseph Aiguier, 13402 Marseille, Cedex 20, France
J Biol Chem 276:18437-41. 2001..To denote this newly assigned function, ykfE has now been registered under the new gene name Ivy (inhibitor of vertebrate lysozyme) at the E. coli genetic stock center...
Structural and genomic correlates of hyperthermostabilityC Cambillau
Architecture et Fonction des Macromolecules Biologiques, CNRS UPR9039, France
J Biol Chem 275:32383-6. 2000..The biophysical requirements for the presence of charged residues at the protein surface, allowing protein stabilization through ion bonds, is therefore clearly imprinted and detectable in all genome sequences available to date...
Identification of odorant receptors from the Alpine marmot (Marmota marmota)V Matarazzo
CNRS UPR 9024, Laboratoire de Neurobiologie, Groupe des Laboratoires de Marseille, France
Cell Mol Biol (Noisy-le-grand) 46:1249-58. 2000..Finally, Northern blot analysis revealed specific expression of these sequences in the Alpine Marmot olfactory epithelium tissue...
Crystallization and preliminary crystallographic study of b0220, an 'ORFan' protein of unknown function from Escherichia coliC Abergel
Information Genetique et Structurale, UMR1889 CNRS AVENTIS, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
Acta Crystallogr D Biol Crystallogr 56:1694-5. 2000..Frozen crystals diffract to 1.6 A resolution using synchrotron radiation. Phasing was performed using multiwavelength anomalous dispersion (MAD) on the selenomethionine-substituted b0220 protein...
Computational methods for the identification of differential and coordinated gene expressionJ M Claverie
Structural and Genetic Information Laboratory, Chemin Joseph Aiguier, Marseille, France
Hum Mol Genet 8:1821-32. 1999..ii) identify genes expressed in a coordinated manner across a set of conditions; and (iii) delineate clusters of genes sharing coherent expression features, eventually defining global biological pathways...
Crystallization and preliminary crystallographic study of HIP/PAP, a human C-lectin overexpressed in primary liver cancersC Abergel
Information Genetique et Structurale, CNRS UMR 1889, Institut de Biologie Structurale et Microbiologie, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
Acta Crystallogr D Biol Crystallogr 55:1487-9. 1999..Flash-frozen crystals diffract to 1. 78 A resolution using synchrotron radiation. A molecular-replacement solution was obtained using the human Reg/lithostathine structure and the AMoRe software...
"Hidden" dUTPase sequence in human immunodeficiency virus type 1 gp120C Abergel
Laboratory of Structural and Genetic Information, CNRS EP 91, Marseille F 13402, France
J Virol 73:751-3. 1999..W. Sweet, J. Sodroski, and W. A. Hendrickson, Nature 393:648-659, 1998), our results strongly suggest that an ancestral dUTPase gene has evolved into the present primate lentivirus CD4 and cytokine receptor interacting region of gp120...
Structural characterization of CA1462, the Candida albicans thiamine pyrophosphokinaseSébastien Santini
Information Genomique et Structurale, UPR2589, Parc Scientifique de Luminy, 13288, Marseille Cedex 09, France
BMC Struct Biol 8:33. 2008..Since thiamine pyrophosphate is an essential product involved in a variety of metabolic pathways, proteins responsible for its production satisfied these two criteria...
Mimivirus reveals Mre11/Rad50 fusion proteins with a sporadic distribution in eukaryotes, bacteria, viruses and plasmidsTakashi Yoshida
Structural and Genomic Information Laboratory, CNRS UPR 2589, Aix Marseille University, Mediterranean Institute of Microbiology, 163 Avenue de Luminy, Case 934, 13288 Marseille Cedex 9, France
Virol J 8:427. 2011..Here we investigated the amino acid sequence of the Mimivirus R555 gene product, originally annotated as a Rad50 homolog, and later shown to have close homologs in marine microbial metagenomes...
Rickettsia felis, from culture to genome sequencingH Ogata
CNRSIBSM, , Marseille, France
Ann N Y Acad Sci 1063:26-34. 2005..It also contains toxin-antitoxin system encoding genes that are extremely rare in intracellular bacteria and may be linked to plasmid maintenance...
[Transcriptome analysis in cancerology: bioinformatics aspects]J M Claverie
, UMR 1889 CNRS-AVENTIS, 31, chemin Joseph-Aiguier, 13402 Marseille Cedex 20
Bull Cancer 88:269-76. 2001..This is the purpose of the present article...
Characterization of mimivirus DNA topoisomerase IB suggests horizontal gene transfer between eukaryal viruses and bacteriaDelphine Benarroch
Molecular Biology Program, Sloan Kettering Institute, New York, NY 10021, USA
J Virol 80:314-21. 2006....
Crystal structure of Escherichia coli DkgA, a broad-specificity aldo-keto reductaseSandra Jeudy
, CNRS, Marseille, France
Proteins 62:302-7. 2006
A giant virus in amoebaeBernard La Scola
, , CNRS UMR 6020, 13385 Marseille, France
Science 299:2033. 2003
Genome-based design of a cell-free culture medium for Tropheryma whippleiPatricia Renesto
, CNRS UMR 6020, , 27 Boulevard Jean Moulin, 13385, Marseille, France
Lancet 362:447-9. 2003..Genomic information can, therefore, provide sufficient clues for designing axenic media for fastidious and uncultured pathogens...
Tropheryma whipplei Twist: a human pathogenic Actinobacteria with a reduced genomeDidier Raoult
Unite des Rickettsies, Faculte de Medecine, CNRS UMR6020, Universite de la Mediterranee, 13385 Marseille Cedex 05, France
Genome Res 13:1800-9. 2003..The analysis of the genome sequence of this previously largely unknown human pathogen is now guiding the development of molecular diagnostic tools and more convenient culture conditions...
Characterization of the naturally occurring oxacillinase of Acinetobacter baumanniiClaire Héritier
Service de Bacteriologie Virologie, Hopital de Bicetre, Le Kremlin Bicetre, France
Antimicrob Agents Chemother 49:4174-9. 2005..By PCR and sequencing bla(OXA-69)-like genes were identified in all A. baumannii strains tested (n = 12), suggesting that this oxacillinase is naturally occurring in that species...
The nucleoside diphosphate kinase from mimivirus: a peculiar affinity for deoxypyrimidine nucleotidesSandra Jeudy
, CNRS UPR 2589, IBSM, 163 Avenue de Luminy, 13288, Marseille Cedex 9, France
J Bioenerg Biomembr 38:247-54. 2006....
Bayesian decomposition analysis of bacterial phylogenomic profilesGhislain Bidaut
Division of Population Science, Bioinformatics, Fox Chase Cancer Center, Philadelphia, PA 19111, USA
Am J Pharmacogenomics 5:63-70. 2005..CONCLUSION: The use of phylogenomic profiles and Bayesian Decomposition provide important insights for the design of new antibacterial therapeutics...
Comparative analysis of Acinetobacters: three genomes for three lifestylesDavid Vallenet
Génomique Métabolique, CNRS UMR8030, CEA Institut de Génomique Genoscope, Evry, France
PLoS ONE 3:e1805. 2008..Taken together, all these observations suggest that the genome contents of the 3 Acinetobacters compared are partly shaped by life in distinct ecological niches: human (and more largely hospital environment), louse, soil...
CaspR: a web server for automated molecular replacement using homology modellingJean-Baptiste Claude
, Institut de Biologie Structurale et Microbiologie, 31, Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
Nucleic Acids Res 32:W606-9. 2004..We expect this automated procedure to have a significant impact on the throughput of large-scale structural genomics projects. CaspR is freely available at http://igs-server.cnrs-mrs.fr/Caspr/...
Some lessons from Rickettsia genomicsPatricia Renesto
, CNRS UMR 6020, IFR-48, , 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
FEMS Microbiol Rev 29:99-117. 2005..Environmental adaptation of Rickettsia is also discussed...
Microbiology. How to infect a MimivirusHiroyuki Ogata
Structural and Genomic Information Laboratory, CNRSUPR 2589, IFR-88, , Parc Scientifique de Luminy, FR-13288 Marseille, France
Science 321:1305-6. 2008
The 1.2-megabase genome sequence of MimivirusDidier Raoult
Unite des Rickettsies, Faculte de Medecine, CNRS UMR6020, Universite de la Mediterranee, 13385 Marseille Cedex 05, France
Science 306:1344-50. 2004..This new sequence data might help shed a new light on the origin of DNA viruses and their role in the early evolution of eukaryotes...
Mimivirus giant particles incorporate a large fraction of anonymous and unique gene productsPatricia Renesto
Unite des Rickettsies, CNRS UMR 6020, IFR 48, Faculte de Medecine, 27 Boulevard Jean Moulin, 13385 Marseille, France
J Virol 80:11678-85. 2006..Thus, anonymous and unique genes constituting the majority of the mimivirus gene complement encode bona fide proteins that are likely to participate in well-integrated processes...
Discovery of protein-coding palindromic repeats in WolbachiaHiroyuki Ogata
Trends Microbiol 13:253-5. 2005
In Ssarch of new anti-bacterial target genes: a comparative/structural genomics approachJean-Michel Claverie
Structural and Genetic Information Laboratory, UMR 1889 CNRS-AVENTIS, 31 Chemin Joseph Aiguier, 13402 Marseille, Cedex 20, France
Comb Chem High Throughput Screen 5:511-22. 2002....
[An extra large giant virus]Jean-Michel Claverie
Med Sci (Paris) 21:15-6. 2005
Genomic correlates of hyperthermostability, an updateKarsten Suhre
Structural and Genomic Information Laboratory, UPR 2589-CNRS, Institute of Structural Biology and Microbiology, CNRS, Marseille, France
J Biol Chem 278:17198-202. 2003..and Suzuki, M. (2000) Proc. Natl. Acad. Sci. 97, 14257-14262). We show that the CvP-bias is the sole criterion that is able to clearly discriminate hyperthermophile from mesothermophile microorganisms on a global genomic basis...
