Ulf-Håkan Stenman

Summary

Affiliation: University of Helsinki
Country: Finland

Publications

  1. Urinary matrix metalloproteinase -8, -9, -14 and their regulators (TRY-1, TRY-2, TATI) in patients with diabetic nephropathy
    Anneli Lauhio
    Helsinki University Central Hospital, Department of Medicine, Division of Infectious Diseases, Helsinki, Finland
    Ann Med 40:312-20
  2. Application of proteomic technology in identifying pancreatic secretory trypsin inhibitor variants in urine of patients with pancreatitis
    Leena Valmu
    Department of Clinical Medicine, Division of Clinical Chemistry, Biomedicum, University of Helsinki, Helsinki, Finland
    Clin Chem 52:73-81
  3. Up-regulation of trypsin and mesenchymal MMP-8 during development of hyperoxic lung injury in the rat
    Katariina Cederqvist
    Department of Pediatrics, Helsinki University Central Hospital and University of Helsinki, FIN 00290 Helsinki, Finland
    Pediatr Res 60:395-400
  4. Trypsin-2 degrades human type II collagen and is expressed and activated in mesenchymally transformed rheumatoid arthritis synovitis tissue
    Mathias Stenman
    Department of Anatomy, Institute of Biomedicine, Helsinki, Finland
    Am J Pathol 167:1119-24
  5. Pulmonary trypsin-2 in the development of bronchopulmonary dysplasia in preterm infants
    Katariina Cederqvist
    Hospital for Children and Adolescents, Helsinki University Central Hospital, Helsinki, Finland
    Pediatrics 112:570-7
  6. Site-specific glycan analysis of human chorionic gonadotropin beta-subunit from malignancies and pregnancy by liquid chromatography--electrospray mass spectrometry
    Leena Valmu
    Department of Clinical Medicine, Division of Clinical Chemistry, Biomedicum, University of Helsinki, PO Box 63 Haartmaninkatu 8, Helsinki FIN 00014, Finland
    Glycobiology 16:1207-18
  7. Relative concentrations of hK2/PSA mRNA in benign and malignant prostatic tissue
    Susanna Lintula
    Department of Clinical Chemistry, Helsinki University Central Hospital, Helsinki, Finland
    Prostate 63:324-9
  8. Placental 11beta-HSD2 activity, early postnatal clinical course, and adrenal function in extremely low birth weight infants
    Eero Kajantie
    Department of Epidemiology and Health Promotion, National Public Health Institute, Helsinki, Finland
    Pediatr Res 59:575-8
  9. Biochemistry and clinical role of trypsinogens and pancreatic secretory trypsin inhibitor
    Annukka Paju
    Department of Clinical Chemistry, Helsinki University Central Hospital, Helsinki, Finland
    Crit Rev Clin Lab Sci 43:103-42
  10. Development of peptides specifically modulating the activity of KLK2 and KLK3
    Hannu Koistinen
    Department of Clinical Chemistry, Biomedicum Helsinki, University of Helsinki and Helsinki University Central Hospital, 00014 Helsinki, Finland
    Biol Chem 389:633-42

Collaborators

Detail Information

Publications59

  1. Urinary matrix metalloproteinase -8, -9, -14 and their regulators (TRY-1, TRY-2, TATI) in patients with diabetic nephropathy
    Anneli Lauhio
    Helsinki University Central Hospital, Department of Medicine, Division of Infectious Diseases, Helsinki, Finland
    Ann Med 40:312-20
    ..Our findings suggest that a trypsin-MMP cascade is involved in the pathogenesis of DNP, which may offer new possibilities for diagnosis and treatment of DNP with MMP inhibitors...
  2. Application of proteomic technology in identifying pancreatic secretory trypsin inhibitor variants in urine of patients with pancreatitis
    Leena Valmu
    Department of Clinical Medicine, Division of Clinical Chemistry, Biomedicum, University of Helsinki, Helsinki, Finland
    Clin Chem 52:73-81
    ..In addition, the N34S variant occurs at higher concentrations than the wild type. This finding casts new light on the possible role of PSTI as a cause of hereditary pancreatitis...
  3. Up-regulation of trypsin and mesenchymal MMP-8 during development of hyperoxic lung injury in the rat
    Katariina Cederqvist
    Department of Pediatrics, Helsinki University Central Hospital and University of Helsinki, FIN 00290 Helsinki, Finland
    Pediatr Res 60:395-400
    ..Marked up-regulation of trypsin and MMP-8 early during hyperoxic lung injury suggests that these enzymes play a role in the pathogenesis of acute lung injury and may therefore be potential targets for therapy of lung injury...
  4. Trypsin-2 degrades human type II collagen and is expressed and activated in mesenchymally transformed rheumatoid arthritis synovitis tissue
    Mathias Stenman
    Department of Anatomy, Institute of Biomedicine, Helsinki, Finland
    Am J Pathol 167:1119-24
    ..Thus, trypsin-2 and its regulators should be further studied for use as markers of prognosis and disease activity in rheumatoid arthritis...
  5. Pulmonary trypsin-2 in the development of bronchopulmonary dysplasia in preterm infants
    Katariina Cederqvist
    Hospital for Children and Adolescents, Helsinki University Central Hospital, Helsinki, Finland
    Pediatrics 112:570-7
    ..This raises the possibility that therapy with exogenous proteinase inhibitors might prevent the development of BPD in preterm infants with respiratory distress...
  6. Site-specific glycan analysis of human chorionic gonadotropin beta-subunit from malignancies and pregnancy by liquid chromatography--electrospray mass spectrometry
    Leena Valmu
    Department of Clinical Medicine, Division of Clinical Chemistry, Biomedicum, University of Helsinki, PO Box 63 Haartmaninkatu 8, Helsinki FIN 00014, Finland
    Glycobiology 16:1207-18
    ..This may facilitate the detection of this malignancy-associated variant by a lectin assay. Analysis of hCGh affinity purified with antibody B152 confirmed that this antibody recognizes a core-2 glycan on Ser-132...
  7. Relative concentrations of hK2/PSA mRNA in benign and malignant prostatic tissue
    Susanna Lintula
    Department of Clinical Chemistry, Helsinki University Central Hospital, Helsinki, Finland
    Prostate 63:324-9
    ..Using this method we have shown that the ratio of hK2/PSA mRNA is higher in cancerous than in benign prostatic tissue...
  8. Placental 11beta-HSD2 activity, early postnatal clinical course, and adrenal function in extremely low birth weight infants
    Eero Kajantie
    Department of Epidemiology and Health Promotion, National Public Health Institute, Helsinki, Finland
    Pediatr Res 59:575-8
    ..Although an association with baseline or ACTH-stimulated cortisol was not seen, possible relationships with other components of the hypothalamic-pituitary-adrenal axis remain to be determined...
  9. Biochemistry and clinical role of trypsinogens and pancreatic secretory trypsin inhibitor
    Annukka Paju
    Department of Clinical Chemistry, Helsinki University Central Hospital, Helsinki, Finland
    Crit Rev Clin Lab Sci 43:103-42
    ..e., the roles of trypsinogens and PSTI/TATI/SPINK1 in spontaneous and hereditary pancreatitis, tumor progression, and prognosis...
  10. Development of peptides specifically modulating the activity of KLK2 and KLK3
    Hannu Koistinen
    Department of Clinical Chemistry, Biomedicum Helsinki, University of Helsinki and Helsinki University Central Hospital, 00014 Helsinki, Finland
    Biol Chem 389:633-42
    ..In this review we summarize the possible roles of KLK3 and KLK2 in prostate cancer and then concentrate on the development of peptides that modulate the activity of these proteases...
  11. Gene expression changes associated with the anti-angiogenic activity of kallikrein-related peptidase 3 (KLK3) on human umbilical vein endothelial cells
    Johanna M Mattsson
    Department of Clinical Chemistry, Biomedicum Helsinki, University of Helsinki and Helsinki University Central Hospital, 00014 University of Helsinki, Finland
    Biol Chem 389:765-71
    ....
  12. Early sequential changes in serum markers of acute pancreatitis induced by endoscopic retrograde cholangiopancreatography
    Marko Lempinen
    Second Department of Surgery, Helsinki University Central Hospital, Helsinki, Finland
    Pancreatology 5:157-64
    ....
  13. Novel small molecule inhibitors for prostate-specific antigen
    Hannu Koistinen
    Department of Clinical Chemistry, Biomedicum Helsinki, University of Helsinki, Helsinki University Central Hospital, Finland
    Prostate 68:1143-51
    ..CONCLUSIONS: We found several PSA inhibitors that could be useful tools for studying the role of PSA in cancer models and in normal physiology as showed in angiogenesis model...
  14. Enhanced detection of cholangiocarcinoma with serum trypsinogen-2 in patients with severe bile duct strictures
    Marko Lempinen
    Clinic of Surgery, Department of Transplantation and Liver Surgery, Helsinki University Hospital, P O Box 263, FIN 00029, Helsinki, Finland
    J Hepatol 47:677-83
    ..759. CONCLUSIONS: Our results suggest that serum trypsinogen-2 is a most useful marker for diagnosing patients with cholangiocarcinoma, and it is superior to serum CA19-9 and CEA...
  15. Targeted gene-expression analysis by genome-controlled reverse transcription-PCR
    Jakob Stenman
    Hospital for Children and Adolescents, University of Helsinki, Helsinki, Finland
    Clin Chem 52:1988-96
    ....
  16. Expression of trypsinogen-1, trypsinogen-2, and tumor-associated trypsin inhibitor in ovarian cancer: prognostic study on tissue and serum
    Annukka Paju
    Department of Clinical Chemistry, Helsinki University Central Hospital, Helsinki, Finland
    Clin Cancer Res 10:4761-8
    ..These results suggest that trypsin expression plays a role in the progression of ovarian cancer. TATI and trypsin-2-API are of potential use as an aid for stratification of randomized studies and for selecting treatment strategies...
  17. Prostate-specific antigen and other prostate-derived proteases cleave IGFBP-3, but prostate cancer is not associated with proteolytically cleaved circulating IGFBP-3
    Hannu Koistinen
    Department of Obstetrics and Gynecology, Biomedicum Helsinki, Helsinki University Central Hospital, Helsinki, Finland
    Prostate 50:112-8
    ..CONCLUSIONS: These results show that, while seminal plasma and prostate-derived proteases can cleave IGFBP-3, in patients with prostate cancer the circulating IGFBP-3 is not significantly proteolyzed by either PSA, hK2, or trypsin...
  18. Separation of enzymatically active and inactive prostate-specific antigen (PSA) by peptide affinity chromatography
    Ping Wu
    Department of Clinical Chemistry, Helsinki University Central Hospital, Helsinki, Finland
    Prostate 58:345-53
    ..CONCLUSIONS: PSA-binding peptides can be used to separate enzymatically active and inactive forms of PSA. Thus the peptides are potentially useful as ligands for development of methods for specific detection of active free PSA...
  19. Estimation of prostate cancer risk on the basis of total and free prostate-specific antigen, prostate volume and digital rectal examination
    Patrik Finne
    Department of Clinical Chemistry, Helsinki University Central Hospital, Biomedicum Helsinki, A418a, P O Box 700 Haartmaninkatu 8, FIN 00029 HUS, Finland
    Eur Urol 41:619-26; discussion 626-7
    ..The nomograms established can be used to avoid or defer biopsy in men with a low prostate cancer probability in spite of a serum PSA level exceeding 4 microg/l...
  20. Structural characterization and anti-angiogenic properties of prostate-specific antigen isoforms in seminal fluid
    Johanna M Mattsson
    Department of Clinical Chemistry, Biomedicum Helsinki, Helsinki University Central Hospital and University of Helsinki, Helsinki, Finland
    Prostate 68:945-54
    ..CONCLUSIONS: Our results show that the anti-angiogenic effect of PSA is based on its proteolytic activity...
  21. Placental 11 beta-hydroxysteroid dehydrogenase-2 and fetal cortisol/cortisone shuttle in small preterm infants
    Eero Kajantie
    Hospital for Children and Adolescents, Helsinki University Central Hospital, 00029 HUS, Helsinki, Finland
    J Clin Endocrinol Metab 88:493-500
    ..Whether these conditions are associated with early postnatal adrenal insufficiency or long-term cardiovascular risk remains an important issue for further study...
  22. New insights into juvenile parotitis
    Kaija Leena Kolho
    Hospital for Children and Adolescents, Helsinki University Central Hospital, Helsinki, Finland
    Acta Paediatr 94:1566-70
    ..It is tempting to speculate that disturbed proteolytic balance may play a role in the development of symptoms...
  23. Dual-label immunoassay for simultaneous measurement of prostate-specific antigen (PSA)-alpha1-antichymotrypsin complex together with free or total PSA
    Lei Zhu
    Department of Clinical Chemistry, Helsinki University Central Hospital, Biomedicum, PB 700, FIN-00029 Helsinki, Finland
    Clin Chem 49:97-103
    ..The same clinical validity can be obtained by determination of fPSA or PSA-ACT together or in combination with tPSA...
  24. Combination of serum hCG beta and p53 tissue expression defines distinct subgroups of serous ovarian carcinoma
    Juhani Vartiainen
    Department of Obstetrics and Gynecology, Helsinki University Central Hospital, Hospital District of Helsinki and Uusimaa, Biomedicum Helsinki, Helsinki, Finland
    Int J Cancer 122:2125-9
    ..Preoperative serum hCG beta and tumor tissue p53 expression are feasible markers that divide serous ovarian carcinomas into clinically relevant subgroups...
  25. Mass spectrometric detection of tyrosine sulfation in human pancreatic trypsinogens, but not in tumor-associated trypsinogen
    Outi Itkonen
    Department of Clinical Chemistry, Helsinki University Central Hospital, Finland
    FEBS J 275:289-301
    ..We conclude that the previously known differences in charge, substrate specificity and inhibitor binding between pancreatic and tumor-associated trypsinogens are probably caused by sulfation of Tyr154 in pancreatic trypsinogens...
  26. Increased expression of tumor-associated trypsin inhibitor, TATI, in prostate cancer and in androgen-independent 22Rv1 cells
    Annukka Paju
    Department of Clinical Chemistry, Helsinki University Central Hospital, Helsinki, Finland
    Eur Urol 52:1670-9
    ..CONCLUSIONS: We demonstrate for the first time that TATI is expressed in the benign and malignant prostate. Increased TATI protein expression is found in high-grade tumors and in 22Rv1 cells in which it is regulated by androgens...
  27. A sensitive proximity ligation assay for active PSA
    Lei Zhu
    Department of Clinical Chemistry, Biomedicum/Helsinki University Central Hospital, FI-00029 Helsinki, Finland
    Biol Chem 387:769-72
    ..Our results show that a highly sensitive immunopeptidometric assay can be developed using proximity ligation. This principle should facilitate establishment of specific assays for active forms of other proteases...
  28. Tumor-associated trypsin inhibitor as a prognostic factor during follow-up of bladder cancer
    Eija Kelloniemi
    Department of Urology, Helsinki University Central Hospital, Helsinki, Finland
    Urology 62:249-53
    ..CONCLUSIONS: Serum TATI is an independent prognostic factor in transitional cell carcinoma and is potentially useful for the identification of patients with an adverse prognosis...
  29. Monoclonal antibodies, immunofluorometric assay, and detection of human semenogelin in male reproductive tract: no association with in vitro fertilizing capacity of sperm
    Hannu Koistinen
    Department of Obstetrics and Gynecology, Helsinki University Central Hospital, 00290 Helsinki, Finland
    Biol Reprod 66:624-8
    ..The levels of semenogelin appear to bear no relationship to the in vitro fertilization capacity of the spermatozoa...
  30. Diabetic pregnancy associated with increased epidermal growth factor in cord serum at term
    Mikko Loukovaara
    Department of Obstetrics and Gynecology, Helsinki University Central Hospital, Haartmaninkatu 2, 00290 Helsinki, Finland
    Obstet Gynecol 103:240-4
    ..We hypothesize that the rise in epidermal growth factor levels is a metabolic response of the fetoplacental unit to diabetes-related hyperglycemia. LEVEL OF EVIDENCE: III..
  31. Serum HCG beta and CA 72-4 are stronger prognostic factors than CEA, CA 19-9 and CA 242 in pancreatic cancer
    Johanna Louhimo
    Department of Surgery, Helsinki University Central Hospital, University of Helsinki, Helsinki, Finland
    Oncology 66:126-31
    ..CONCLUSIONS: All the tumor markers studied (CEA, CA 19-9, CA 242, CA 72-4, and hCG beta) had prognostic value in pancreatic cancer, and hCG beta, CA 72-4, and stage were the strongest independent prognostic factors in this study...
  32. Algorithms based on prostate-specific antigen (PSA), free PSA, digital rectal examination and prostate volume reduce false-positive PSA results in prostate cancer screening
    Patrik Finne
    Department of Clinical Chemistry, University of Helsinki, Helsinki, Finland
    Int J Cancer 111:310-5
    ..718). A multivariate algorithm can be used to reduce unnecessary prostate biopsies in screening more effectively than the proportion of free PSA alone, but the algorithms will require updating when clinical practice develops with time...
  33. Failure of second-trimester measurement of soluble endoglin and other angiogenic factors to predict placental abruption
    Minna Tikkanen
    Department of Obstetrics and Gynecology, University Central Hospital, Helsinki, Finland
    Prenat Diagn 27:1143-6
    ..Similarly, sFlt-1, PlGF, or sFlt-1/PlGF ratio showed no difference between the cases and the controls. CONCLUSION: Our data suggest that sEng, PlGF and sFlt-1 levels in early second trimester fail to predict placental abruption...
  34. Epitope mapping of antibodies against prostate-specific antigen with use of peptide libraries
    Jari Leinonen
    Department of Clinical Chemistry, Helsinki University Central Hospital, Haartmaninkatu 8, FIN 0029 Helsinki, Finland
    Clin Chem 48:2208-16
    ..Peptides reactive with two MAbs recognizing the same epitope can be obtained by sequential panning. This method can be used to predict the location of some epitopes, but additional methods are needed to confirm the result...
  35. Tissue AP-2gamma and Oct-3/4, and serum CA 125 as diagnostic and prognostic markers of malignant ovarian germ cell tumors
    Jonna Salonen
    Children s Hospital and Program for Women s Health, Biomedicum Helsinki, University of Helsinki, Helsinki, Finland
    Tumour Biol 29:50-6
    ..Taken together, increased preoperative serum CA 125 levels indicate poor prognosis of MOGCTs. Tissue AP-2gamma and Oct-3/4 are associated with dysgerminomas and can thus be used as additional differential diagnostic tools in MOGCTs...
  36. Trypsinogen-2 and trypsinogen activation peptide (TAP) in urine of patients with acute pancreatitis
    Marko Lempinen
    Second Department of Surgery, Helsinki University Central Hospital, Helsinki, Finland
    J Surg Res 111:267-73
    ..These results suggest that it could be a valuable adjunct in the early assessment of the severity of acute pancreatitis...
  37. Expression of vascular endothelial growth factor in peripheral blood cells of preeclamptic women
    Piia Vuorela
    Department of Obstetrics and Gynecology, Helsinki University Central Hospital, Biomedicum, Helsinki, Finland
    Hypertens Pregnancy 22:193-201
    ..07). CONCLUSION: Uncomplicated pregnancy is associated with an elevated VEGF content of mononuclear cells. Preeclampsia does not seem to affect the VEGF content of maternal peripheral blood mononuclear cells, granulocytes, or platelets...
  38. Novel peptide inhibitors of human kallikrein 2
    Can Hekim
    Department of Clinical Chemistry, Helsinki University Central Hospital, Biomedicum, FIN-00290 Helsinki, Finland
    J Biol Chem 281:12555-60
    ..Amino acid substitution analyses revealed that motifs of six amino acids were required for the inhibitory activity. These peptides are potentially useful for treatment and targeting of prostate cancer...
  39. Activity and stability of human kallikrein-2-specific linear and cyclic peptide inhibitors
    Miikka Pakkala
    Department of Chemistry, University of Kuopio, P O Box 1627, FIN 70211, Kuopio, and Department of Clinical Chemistry, Helsinki University Central Hospital, Finland
    J Pept Sci 13:348-53
    ..This should render the peptides more useful for in vivo applications, such as tumor imaging and prostate cancer targeting...
  40. Characterization of monoclonal antibodies against prostate specific antigen produced by genetic immunization
    Jari Leinonen
    Department of Clinical Chemistry in Biomedicum, Helsinki University Central Hospital, Finland
    J Immunol Methods 289:157-67
    ..As applied in the present study, it is less efficient than protein immunization, but it is a useful technique when the antigen is not available in the quantities needed for immunization...
  41. Overexpression of human chorionic gonadotropin beta genes 3, 5 and 8 in tumor tissue and urinary cells of bladder cancer patients
    Kristina Hotakainen
    Department of Clinical Chemistry, Helsinki University Central Hospital and University of Helsinki, Helsinki, Finland
    Tumour Biol 28:52-6
    ..Quantification of the relative mRNA expression levels of the hCGbeta type I and II genes in urine cells should be further studied as a potential noninvasive tool for the diagnosis and follow-up of bladder cancer...
  42. Glycodelin reduces breast cancer xenograft growth in vivo
    Laura C Hautala
    Department of Obstetrics and Gynecology, Helsinki University Central Hospital, Biomedicum Helsinki, University of Helsinki, Helsinki, Finland
    Int J Cancer 123:2279-84
    ..Our results suggest that glycodelin acts as a tumor suppressor in breast cancer. This may explain its reported association with a more favorable prognosis in some cancers...
  43. Elevated hCG and a high proportion of hCGbeta in serum preceding the diagnosis of trophoblastic disease by seven months
    Juhani Vartiainen
    Department of Obstetrics and Gynaecology, Helsinki University Central Hospital, Finland
    BJOG 109:589-90
  44. Gonadotropins in doping: pharmacological basis and detection of illicit use
    U H Stenman
    Department of Clinical Chemistry, Helsinki University Central Hospital, HUS, Finland
    Br J Pharmacol 154:569-83
    ..It is therefore difficult to detect illicit use of LH. The characteristics and reference values for hCG and LH assays used in doping control and the cutoff values need to be defined...
  45. Pancreatic secretory trypsin inhibitor (SPINK1) gene mutations in patients with acute pancreatitis
    Eija Tukiainen
    Second Department of Surgery, University of Helsinki and Helsinki University Central Hospital, Helsinki, Finland
    Pancreas 30:239-42
    ..2%), but the differences were not statistically significant. No differences in age at admission and number of attacks of AP were observed between the groups. CONCLUSION: SPINK1 N34S mutation enhances the susceptibility of AP...
  46. Fetal hypoxia is associated with elevated cord serum C-reactive protein levels in diabetic pregnancies
    Mikko Loukovaara
    Department of Obstetrics and Gynecology, Helsinki University Central Hospital, Helsinki, Finland
    Biol Neonate 85:237-42
    ..Amniotic fluid erythropoietin showed an independent effect on cord serum CRP in multiple regression analysis. These data suggest that the fetus responds to hypoxia by an inflammatory reaction...
  47. Determination of aldosterone in serum by liquid chromatography-tandem mass spectrometry
    Ursula Turpeinen
    HUSLAB, Laboratory of Women s Clinic, P O Box 140, 00029 HUS, Helsinki, Finland
    J Chromatogr B Analyt Technol Biomed Life Sci 862:113-8
    ..The sensitivity is adequate for patient samples. Aldosterone concentrations reported by nonextraction RIA were consistently higher than those produced by LC-MS/MS...
  48. Mutations N34S and P55S of the SPINK1 gene in patients with chronic pancreatitis or pancreatic cancer and in healthy subjects: a report from Finland
    Marko Lempinen
    Fourth Department of Surgery, Helsinki University Central Hospital, FI 00029 Helsinki, Finland
    Scand J Gastroenterol 40:225-30
    ..The association of the N34S mutation with alcoholic CP was marginally stronger than in earlier studies, whereas in the Finnish population in general, this mutation was significantly more frequent than reported elsewhere...
  49. Preoperative hCGbeta and CA 72-4 are prognostic factors in gastric cancer
    Johanna Louhimo
    Department of Surgery, Helsinki University Central Hospital, University of Helsinki, Helsinki, Finland
    Int J Cancer 111:929-33
    ..In gastric cancer, tumour markers hCGbeta and CA 72-4 are independent prognostic factors in addition to stage and histological type of the tumour...