Research Topics
Species | P NeubauerSummaryAffiliation: University of Oulu Country: Finland Publications
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Detail Information
Publications
Biotechnology - 12th European CongressPeter Neubauer
University of Oulu, Department of Process and Environmental Engineering, Bioprocess Engineering Laboratory, Finland
IDrugs 8:809-12. 2005- Isolation and characterization of a T7-like lytic phage for Pseudomonas fluorescensSanna Sillankorva
IBB Institute for Biotechnology and Bioengineering, Centre of Biological, Engineering, Universidade do Minho, Campus de Gualtar 4710 057, Braga, Portugal
BMC Biotechnol 8:80. 2008..Here we describe the isolation and characterization of a lytic phage capable to infect a variety of P. fluorescens strains isolated from Portuguese and United States dairy industries... - Improved production of human type II procollagen in the yeast Pichia pastoris in shake flasks by a wireless-controlled fed-batch systemMaria Ruottinen
Bioprocess Engineering Laboratory, Dept, of Process and Environmental Engineering, University of Oulu, P O Box 4300, FIN 90014 University of Oulu, Finland
BMC Biotechnol 8:33. 2008..The development of feeding protocols and the study of AOX1-controlled recombinant protein production have been largely made in shake flasks, although shake flasks have very limited possibilities for measurement and control... - Effective inhibition of lytic development of bacteriophages lambda, P1 and T4 by starvation of their host, Escherichia coliMarcin Łoś
Department of Molecular Biology, University of Gdansk, Kładki 24, 80 822 Gdansk, Poland
BMC Biotechnol 7:13. 2007..Therefore, a simple method for quick inhibition of phage development after detection of bacterial culture infection should be very useful... - Pseudomonas fluorescens biofilms subjected to phage phiIBB-PF7ASanna Sillankorva
IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar 4710 057, Braga, Portugal
BMC Biotechnol 8:79. 2008..Here we describe the application of phage phiIBB-PF7, a newly isolated phage, to control P. fluorescens biofilms. The biofilms were formed under static or dynamic conditions and with or without renewal of medium... - Metabolic load of recombinant protein production: inhibition of cellular capacities for glucose uptake and respiration after induction of a heterologous gene in Escherichia coliP Neubauer
Bioprocess Engineering Laboratory, P O Box 4300, Department of Process and Environmental Engineering, Biocenter Oulu, University of Oulu, FIN 90014 Oulu, Finland
Biotechnol Bioeng 83:53-64. 2003..2001] Biotechnol Bioeng 73:349-357). The new model also includes the population characteristics and gives a good fit to the measured data describing growth, production, substrate consumption, by-product formation, and respiration... - Norvaline is accumulated after a down-shift of oxygen in Escherichia coli W3110Jaakko Soini
Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering and Biocenter Oulu, University of Oulu, P, O, Box 4300, FI 90014 Oulu, Finland
Microb Cell Fact 7:30. 2008..abstract:.. - High cell density media for Escherichia coli are generally designed for aerobic cultivations - consequences for large-scale bioprocesses and shake flask culturesJaakko Soini
Bioprocess Engineering Laboratory, Dept, of Process and Environmental Engineering, University of Oulu, P, O, Box 4300, FIN 90014 Oulu, Finland
Microb Cell Fact 7:26. 2008..abstract:.. - 16S rRNA targeted sandwich hybridization method for direct quantification of mycobacteria in soilsTimo Nieminen
Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering and Biocenter Oulu, University of Oulu, Finland
J Microbiol Methods 67:44-55. 2006..The 16S rRNA sandwich hybridization method described in this paper offers a culture independent solution for tracking environmental reservoirs of viable and potentially infectious mycobacteria... - Octaketide-producing type III polyketide synthase from Hypericum perforatum is expressed in dark glands accumulating hypericinsKatja Karppinen
Department of Biology, University of Oulu, Finland
FEBS J 275:4329-42. 2008..Based on our results, HpPKS2 may have a role in the biosynthesis of hypericins in H. perforatum but some additional factors are possibly required for the production of emodin anthrone in vivo... - Efficient lactic acid production from high salt containing dairy by-products by Lactobacillus salivarius ssp. salicinius with pre-treatment by proteolytic microorganismsAntti Vasala
Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering, University of Oulu, FIN 90014 Oulu, Finland
J Biotechnol 117:421-31. 2005..The results show that fast and complete conversion of lactose to lactic acid can be obtained in dairy by-products without expensive additives... - LC/MS/MS identification of glycosides produced by biotransformation of cinnamyl alcohol in Rhodiola rosea compact callus aggregatesAri Tolonen
Department of Chemistry, University of Oulu, PO Box 3000, 90014 Oulu, Finland
Biomed Chromatogr 18:550-8. 2004.... - Murine Wnt-1 with an internal c-myc tag recombinantly produced in Escherichia coli can induce intracellular signaling of the canonical Wnt pathway in eukaryotic cellsBeatrix Fahnert
Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering, University of Oulu, P O Box 4300, FIN 90014 Oulu, Finland
J Biol Chem 279:47520-7. 2004..Thus, this approach could make recombinant murine Wnt-1 available as a good starting point for other Wnts needed, for example, for maintaining and differentiating stem cells, organ restoration therapy, and tissue engineering... - Inclusion bodies: formation and utilisationBeatrix Fahnert
Biocenter Oulu, Department of Process and Environmental Engineering, Bioprocess Engineering Laboratory, University of Oulu, PO Box 4300, 90014 Oulu, Finland
Adv Biochem Eng Biotechnol 89:93-142. 2004..In addition, simple strategies have been developed to optimise the purification and renaturation of disulfide bond containing proteins making a fast transfer of such processes into the industrial production scale realistic... - Two-dimensional proteome reference map for the radiation-resistant bacterium Deinococcus geothermalisChristina Liedert
Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering and Biocenter Oulu, University of Oulu, Linnanmaa, Oulu, Finland
Proteomics 10:555-63. 2010..A total of 34 abundant proteins with unknown function may relate to the extreme stress tolerance of D. geothermalis... - Quality control of inclusion bodies in Escherichia coliBritta Jürgen
Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering and Biocenter Oulu, University of Oulu, FIN 90014 Oulu, Finland
Microb Cell Fact 9:41. 2010..IBs mostly contain only minor impurities and are relatively homogenous... - Sandwich ELISA for quantitative detection of human collagen prolyl 4-hydroxylaseEkaterina Osmekhina
Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering, P O Box 4300, FIN 90014 University of Oulu, Finland
Microb Cell Fact 9:48. 2010..Then the following holoenzyme detection is accomplished by a goat anti-rabbit IgG labeled with alkaline phosphatase which AP catalyzes the reaction of a substrate transformation with fluorescent signal generation... - High-level production of human collagen prolyl 4-hydroxylase in Escherichia coliAntje Neubauer
Collagen Research Unit, Biocenter Oulu and Department of Medical Biochemistry and Molecular Biology, P. O. Box 5000, University of Oulu, FIN-90014 Oulu, Finland
Matrix Biol 24:59-68. 2005..The enzyme expressed in E. coli differed from those present in vivo and those produced in other hosts in that it lacked the N glycosylation of its alpha subunits, which may be advantageous in crystallization experiments... - Fermentation process for tetrameric human collagen prolyl 4-hydroxylase in Escherichia coli: improvement by gene optimisation of the PDI/beta subunit and repeated addition of the inducer anhydrotetracyclineAntje Neubauer
Collagen Research Unit, Biocenter Oulu and Department of Medical Biochemistry and Molecular Biology, P O Box 5000, University of Oulu, FIN 90014 Oulu, Finland
J Biotechnol 128:308-21. 2007..The optimisation approach resulted in a fermentation yield of 143 mg L(-1) of active C-P4H, corresponding to approximately 7.5% of the total soluble cell protein... - RNA-based sandwich hybridisation method for detection of lactic acid bacteria in brewery samplesSanna Huhtamella
Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering, Biocenter Oulu, University of Oulu, P O Box 4300, FI 90014 Oulu, Finland
J Microbiol Methods 68:543-53. 2007.... - Modelling of translation of human protein disulfide isomerase in Escherichia coli-A case study of gene optimisationOlli Niemitalo
Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering, University of Oulu, Oulu, Finland
J Biotechnol 120:11-24. 2005..coli translational ribosome traffic based upon the stochastic Gillespie algorithm. The study indicates the applicability of such models in optimisation of recombinant protein sequences... - A novel fed-batch based cultivation method provides high cell-density and improves yield of soluble recombinant proteins in shaken culturesMirja Krause
BioSilta Oy, c o Department of Biochemistry, University of Oulu, Finland
Microb Cell Fact 9:11. 2010..In this article we describe the impact of a novel enzymatically controlled fed-batch cultivation technology on recombinant protein production in Escherichia coli in simple shaken cultures... - Structure-based protein engineering efforts with a monomeric TIM variant: the importance of a single point mutation for generating an active site with suitable binding propertiesMarkus Alahuhta
Biocenter Oulu, University of Oulu, Oulu, Finland
Protein Eng Des Sel 21:257-66. 2008..The binding of this citrate molecule is stabilized by hydrogen bonding interactions with the new binding groove... - Structural studies show that the A178L mutation in the C-terminal hinge of the catalytic loop-6 of triosephosphate isomerase (TIM) induces a closed-like conformation in dimeric and monomeric TIMMarkus Alahuhta
Biocenter Oulu and Department of Biochemistry, University of Oulu, PO Box 3000, FIN 90014 University of Oulu, Finland
Acta Crystallogr D Biol Crystallogr 64:178-88. 2008..In the liganded structures of A178L and ml1A178L the structural differences induced by the mutation are minimal. Structural characterization of the ml1A178L variant highlights its structural plasticity... - A role for bacteriophage T4 rI gene function in the control of phage development during pseudolysogeny and in slowly growing host cellsMarcin Los
Biocenter Oulu and Department of Process and Environmental Engineering, University of Oulu, Oulu, Finland
Res Microbiol 154:547-52. 2003..The rI gene, previously known to be indispensable for lysis inhibition, seems to play an important role in optimization of phage development in slowly growing cells as well as during establishment and maintenance of pseudolysogeny... - Isolation and genotype-dependent, organ-specific expression analysis of a Rhodiola rosea cDNA encoding tyrosine decarboxylaseZsuzsanna György
Department of Process and Environmental Engineering, University of Oulu, 90014 Oulu, Finland
J Plant Physiol 166:1581-6. 2009..The difference in the expression intensity suggests a decisive role for this enzyme in the salidroside biosynthesis... - Expression of Escherichia coli glutaredoxin 2 is mainly regulated by ppGpp and sigmaSAristi Potamitou
Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 171 77 Stockholm, Sweden
J Biol Chem 277:17775-80. 2002..Furthermore, Grx2 levels were elevated in an oxyR(-) strain. In accordance with the role of Grx2 as a stationary phase protein, null mutants for grxB were shown to lyse under starvation conditions and exhibited a distorted morphology... - Change of extracellular cAMP concentration is a sensitive reporter for bacterial fitness in high-cell-density cultures of Escherichia coliHongying Lin
, , Halle, Germany
Biotechnol Bioeng 87:602-13. 2004.... - A novel monothiol glutaredoxin (Grx4) from Escherichia coli can serve as a substrate for thioredoxin reductaseAristi Potamitou Fernandes
Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 171 77 Stockholm, Sweden
J Biol Chem 280:24544-52. 2005..Grx4 was highly elevated upon iron depletion, suggesting an iron-related function for the protein...