Evaluation of the bacterial diversity in the feces of cattle using 16S rDNA bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP)Scot E Dowd
USDA ARS Livestock Issues Research Unit, Lubbock, TX 79403, USA
BMC Microbiol 8:125. 2008
..We have evaluated the microbiome from the feces of 20 commercial, lactating dairy cows...
- Evaluation of the bacterial diversity among and within individual venous leg ulcers using bacterial tag-encoded FLX and titanium amplicon pyrosequencing and metagenomic approaches
Randall D Wolcott
Southwest Regional Wound Care Clinic, Lubbock, TX 79410, USA
BMC Microbiol 9:226. 2009
..There are known underlying pathologies which contribute to the chronic nature of VLU including biofilm phenotype infections...
- The complexity of Rhipicephalus (Boophilus) microplus genome characterised through detailed analysis of two BAC clones
Paula M Moolhuijzen
Centre for Comparative Genomics, Murdoch University, South St, Perth, Western Australia, 6150, Australia
BMC Res Notes 4:254. 2011
..abstract:..
- The effect of the macrolide antibiotic tylosin on microbial diversity in the canine small intestine as demonstrated by massive parallel 16S rRNA gene sequencing
Jan S Suchodolski
Gastrointestinal Laboratory, Texas A and M University, College Station, TX, USA
BMC Microbiol 9:210. 2009
..Jejunal brush samples were collected through the fistula on days 0, 14, and 28 (14 days after withdrawal of tylosin). Bacterial diversity was characterized using massive parallel 16S rRNA gene pyrosequencing...
- Assessment of bacterial diversity in the cattle tick Rhipicephalus (Boophilus) microplus through tag-encoded pyrosequencing
Renato Andreotti
USDA ARS Knipling Bushland U S Livestock Insects Research Laboratory, 2700 Fredericksburg Rd, Kerrville, TX 78028, USA
BMC Microbiol 11:6. 2011
..Pyrosequencing was performed on adult males and females, eggs, and gut and ovary tissues from adult females derived from samples of R. microplus collected during outbreaks in southern Texas...
- Evaluation of the bacterial diversity of pressure ulcers using bTEFAP pyrosequencing
Drake M Smith
Research and Testing Laboratory, Lubbock, TX 79407, USA
BMC Med Genomics 3:41. 2010
..The microflora of chronic wounds such as ulcers most commonly exist in the biofilm phenotype and have been known to significantly impair normal healing trajectories...
- Gene expression profiling within the spleen of Clostridium perfringens-challenged broilers fed antibiotic-medicated and non-medicated diets
Aimie J Sarson
Guelph Food Research Centre, Agriculture and Agri Food Canada, Guelph, Canada
BMC Genomics 10:260. 2009
..We therefore investigated gene expression profiles within immunologically-relevant tissue, the spleen, in order to identify factors that are involved in immunity to NE and have potential as therapeutic targets...
- Gene expression profiling in chicken heterophils with Salmonella enteritidis stimulation using a chicken 44 K Agilent microarray
Hsin I Chiang
Department of Poultry Science, Texas A and M University, College Station, TX 77843, USA
BMC Genomics 9:526. 2008
..To dissect the interplay between heterophils and SE infection, we utilized large-scale gene expression profiling...
- Microarray analysis and draft genomes of two Escherichia coli O157:H7 lineage II cattle isolates FRIK966 and FRIK2000 investigating lack of Shiga toxin expression
Scot E Dowd
Research and Testing Laboratory, Lubbock, Texas 79407, USA
Foodborne Pathog Dis 7:763-73. 2010
..The microarray and genome data have been made available to the scientific community to allow continuing analysis of these cattle-isolated lineage II genomes and their gene expression...
- Polymicrobial nature of chronic diabetic foot ulcer biofilm infections determined using bacterial tag encoded FLX amplicon pyrosequencing (bTEFAP)
Scot E Dowd
Research and Testing Laboratory, Lubbock, Texas, USA
PLoS ONE 3:e3326. 2008
..Diabetic foot ulcers are the most common, disabling, and costly complications of diabetes. Here we seek to derive a better understanding of the polymicrobial nature of chronic diabetic extremity ulcer infections...
- Survey of fungi and yeast in polymicrobial infections in chronic wounds
S E Dowd
Research and Testing Laboratory, Lubbock, TX, USA
J Wound Care 20:40-7. 2011
..To assess the incidence, abundance and species diversity of fungi in chronic wounds, as well as to describe the associations of major fungi populations...
- Molecular diagnostics and personalised medicine in wound care: assessment of outcomes
S E Dowd
Research and Testing Laboratory and Pathogenius Diagnostics, Lubbock, TX, USA
J Wound Care 20:232, 234-9. 2011
....
- Healing and healing rates of chronic wounds in the age of molecular pathogen diagnostics
R D Wolcott
Southwest Regional Wound Care Center, Lubbock, Texas, USA
J Wound Care 19:272-8, 280-1. 2010
..To compare healing outcomes at a wound healing centre both before and after the introduction of molecular pathogen diagnostics...
- Propagation of anaerobic bacteria within an aerobic multi-species chronic wound biofilm model
Y Sun
Research and Testing Laboratory, Lubbock, TX, USA
J Wound Care 18:426-31. 2009
..In order to better screen antimicrobial and antibiofilm therapeutics, we evaluated the ability of key anaerobes to incorporate and propagate within our aerobic chronic wound biofilm...
- Bacterial diversity in surgical site infections: not just aerobic cocci any more
R D Wolcott
Southwest Regional Wound Care Centre, Lubbock, Texas, USA
J Wound Care 18:317-23. 2009
..To evaluate the microbial diversity in chronic surgical site infections (SSIs)...
- A rapid molecular method for characterising bacterial bioburden in chronic wounds
R D Wolcott
Southwest Regional Wound Care Center, Lubbock, TX, USA
J Wound Care 17:513-6. 2008
..This case study describes how, unlike traditional culture reports, a modification of this technique rapidly determined that Pseudomonas spp. was present in a chronic wound...
- Biofilm maturity studies indicate sharp debridement opens a time- dependent therapeutic window
R D Wolcott
Southwest Regional Wound Care Center, Lubbock, TX, USA
J Wound Care 19:320-8. 2010
..To investigate the hypothesis that newly formed wound biofilms (or bioburdens) are more susceptible to antimicrobial treatment...
- Identification of yeast in chronic wounds using new pathogen-detection technologies
J L Leake
Texas Tech University Howard Hughes Medical Institute Program, Lubbock, Texas, USA
J Wound Care 18:103-4, 106, 108. 2009
..To evaluate the ability of two new diagnostic methods to detect and accurately identify yeast associated with chronic wound infections...
- Chronic wounds and the medical biofilm paradigm
R D Wolcott
Southwest Regional Wound Care Center, Lubbock, Texas, USA
J Wound Care 19:45-6, 48-50, 52-3. 2010
..There is a growing recognition that biofilms are the principal cause of wound chronicity. The development of treatments for wound biofilms raises the prospect that chronic wounds can be treated, potentially saving many patients' lives...
- Effects of biofilm treatments on the multi-species Lubbock chronic wound biofilm model
S E Dowd
US Department of Agriculture ARS Livestock Issues Research Unit, Lubbock, Texas, USA
J Wound Care 18:508, 510-12. 2009
..To evaluate the efficacy of several biofilm effectors in inhibiting biofilm formation in an in vitro multi-species chronic wound biofilm model...
- Regular debridement is the main tool for maintaining a healthy wound bed in most chronic wounds
R D Wolcott
Southwest Regional Wound Care Center, Lubbock, Texas, USA
J Wound Care 18:54-6. 2009
..Sharp debridement is the most clinically and cost-effective way of physically removing and suppressing a biofilm. Continued debridement, as part of a multifaceted treatment strategy, will keep the biofilm in a weakened state...
- Bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP) for microbiome studies: bacterial diversity in the ileum of newly weaned Salmonella-infected pigs
Scot E Dowd
USDA ARS Livestock Issues Research Unit, Lubbock, Texas 79403, USA
Foodborne Pathog Dis 5:459-72. 2008
..Using bTEFAP, we can expect to gain a better understanding of how the microbiome of an animal contributes to its health and well-being...
- Comparison of Shiga-like toxin II expression between two genetically diverse lineages of Escherichia coli O157:H7
Scot E Dowd
U S Department of Agriculture, Agricultural Research Service Livestock Issues Research Unit, 1604 West FM 1294, Lubbock, Texas 79403, USA
J Food Prot 71:1673-8. 2008
..coli O157 populations to differentiate these lineages. This study provides useful information on the ecology of E. coli O157, with broad implications within the clinical, scientific, and livestock industries...
- Biofilms and chronic wound inflammation
R D Wolcott
Southwest Regional Wound Care Center, Lubbock, Texas, USA
J Wound Care 17:333-41. 2008
..In contrast to the commonly accepted hypothesis of host-centred pathology, it is possible that surface bacteria, not host dysfunction, cause the chronicity and perpetual inflammation associated with chronic non-healing wounds...
- Microarray based comparison of two Escherichia coli O157:H7 lineages
Scot E Dowd
Livestock Issues Research Unit, USDA ARS, Lubbock, TX, USA
BMC Microbiol 6:30. 2006
..To determine if there are virulence related genes differentially expressed between the two lineages we chose to utilize microarray technology to perform an initial screening...
- Survey of bacterial diversity in chronic wounds using pyrosequencing, DGGE, and full ribosome shotgun sequencing
Scot E Dowd
United States Department of Agriculture ARS Livestock Issues Research Unit, Lubbock, TX, USA
BMC Microbiol 8:43. 2008
....
- Evaluation of gene expression in a single antibiotic exposure-derived isolate of Salmonella enterica typhimurium 14028 possessing resistance to multiple antibiotics
Scot E Dowd
Livestock Issues Research Unit, U S Department of Agriculture, Agricultural Research Service, Lubbock, Texas 79403, USA
Foodborne Pathog Dis 5:205-21. 2008
..Future work in sequencing these genomes and evaluating pathogenicity are suggested...
- Positive adaptive state: microarray evaluation of gene expression in Salmonella enterica Typhimurium exposed to nalidixic acid
Scot E Dowd
United States Department of Agriculture, Agricultural Research Service, Livestock Issues Research Unit, Lubbock, Texas 79403, USA
Foodborne Pathog Dis 4:187-200. 2007
..Evaluation of gene expression profile changes that occur during exposure to antibiotics will continue to improve our understanding of the development of antibiotic resistance...
- Escherichia coli O157:H7 gene expression in the presence of catecholamine norepinephrine
Scot E Dowd
United States Department of Agriculture, Agriculture Research Service, Livestock Issues Research Unit, Lubbock, TX 79403, USA
FEMS Microbiol Lett 273:214-23. 2007
..coli O157:H7. The results corroborate many of the previous findings detailed in the literature and provide new observations that could expand the scope of microbial endocrinology...
- Characterization of a newly developed chicken 44K Agilent microarray
Xianyao Li
Texas Agricultural Experiment Station, Texas A and M University, College Station, TX, USA
BMC Genomics 9:60. 2008
..This provides a flexible and powerful platform with high sensitivity and specificity for gene expression studies...
- Handling may cause increased shedding of Escherichia coli and total coliforms in pigs
Scot E Dowd
Livestock Issues Research Unit, USDA Agricultural Research Service, Lubbock, TX 79403, USA
Foodborne Pathog Dis 4:99-102. 2007
..coli and total coliforms, possibly due to stress, and these populations of bacteria may have potential utility as inexpensive, noninvasive indicators of handling-related stress in pigs...
- Choline and osmotic-stress tolerance induced in Arabidopsis by the soil microbe Bacillus subtilis (GB03)
Huiming Zhang
Department of Chemistry, Texas Tech University, Lubbock, TX, USA
Mol Plant Microbe Interact 23:1097-104. 2010
..These results provide a biological strategy to enhance Cho biosynthesis in plants and, in turn, increase plant tolerance to osmotic stress by elevating osmoprotectant accumulation...
- In vitro multispecies Lubbock chronic wound biofilm model
Yan Sun
Medical Biofilm Research Institute, Lubbock, Texas 79410, USA
Wound Repair Regen 16:805-13. 2008
..We have defined a realistic in vitro multispecies biofilm model simulating the functional characteristics of chronic pathogenic biofilms and developed effective tools for its characterization and analyses...
- Windows .NET Network Distributed Basic Local Alignment Search Toolkit (W.ND-BLAST)
Scot E Dowd
Livestock Issues Research Unit, Agriculture Research Service, USDA, Lubbock, TX, USA
BMC Bioinformatics 6:93. 2005
..Our goal was to develop an easy to use, fault tolerant, high-throughput BLAST solution that incorporates a comprehensive BLAST result viewer with curation and annotation functionality...
- Confirmed detection of Cyclospora cayetanesis, Encephalitozoon intestinalis and Cryptosporidium parvum in water used for drinking
Scot E Dowd
United States Department of Agriculture, Agricultural Research Service, Livestock Issues Unit, Texas Technological University, Lubbock, TX 79409, USA
J Water Health 1:117-23. 2003
..The fact that the waters tested may be used for human consumption indicates that these emerging protozoa may be transmitted by ingestion of contaminated water...
- Iron regulated genes of Salmonella enterica serovar Typhimurium in response to norepinephrine and the requirement of fepDGC for norepinephrine-enhanced growth
Bradley L Bearson
Swine Odor and Manure Management Research Unit, USDA, ARS, National Soil Tilth Laboratory, Ames, IA 50011, USA
Microbes Infect 10:807-16. 2008
..Contrary to previous reports using small animal models, the fepA iroN cirA mutant of Salmonella Typhimurium colonized the swine gastrointestinal tract, as did the fepC mutant...
- Detection of protozoan parasites and microsporidia in irrigation waters used for crop production
Jeanette A Thurston-Enriquez
US Department of Agriculture, Agricultural Research Service, University of Nebraska, Lincoln 68583-0934, USA
J Food Prot 65:378-82. 2002
..The presence of human pathogenic parasites in irrigation waters used in the production of crops traditionally consumed raw suggests that there may be a risk of infection to consumers who come in contact with or eat these products...
