Research Topics
| S BuusSummaryAffiliation: University of Copenhagen Country: Denmark Publications
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Detail Information
Publications
Large-scale validation of methods for cytotoxic T-lymphocyte epitope predictionMette V Larsen
Center for Biological Sequence Analysis, BioCentrum DTU, Building 208, Technical University of Denmark, DK 2800 Lyngby, Denmark
BMC Bioinformatics 8:424. 2007..Here, we develop such large-scale benchmark and corresponding performance measures and report the performance of an updated version 1.2 of NetCTL in comparison with the four other methods...- Sensitive quantitative predictions of peptide-MHC binding by a 'Query by Committee' artificial neural network approachS Buus
Division of Experimental Immunology, Institute of Medical Microbiology and Immunology, University of Copenhagen, Denmark
Tissue Antigens 62:378-84. 2003..Finally, it is an example of an iterative feedback loop whereby advanced, computational bioinformatics optimize experimental strategy, and vice versa... - Tumor-associated antigens identified by mRNA expression profiling induce protective anti-tumor immunityS Mathiassen
Institute of Medical Anatomy, University of Copenhagen, Copenhagen, Denmark
Eur J Immunol 31:1239-46. 2001..This may enable a multi-epitope based immunotherapy with improved prospects of clinical tumor rejection... - Addition of TAT protein transduction domain and GrpE to human p53 provides soluble fusion proteins that can be transduced into dendritic cells and elicit p53-specific T-cell responses in HLA-A*0201 transgenic miceS Justesen
Institute of International Health, Immunology and Microbiology, The Panum Institute, University of Copenhagen, Denmark
Immunology 122:326-34. 2007..The use of rTAT-p53 in ongoing clinical protocols should be applicable and offers advantages to current strategies omitting the use of HLA-typed patients... - SARS CTL vaccine candidates; HLA supertype-, genome-wide scanning and biochemical validationC Sylvester-Hvid
Division of Experimental Immunology, Institute of Medical Microbiology and Immunology (IMMI, University of Copenhagen, Building 18.3, Panum Institute, Blegdamsvej 3, 2200 Copenhagen N, Denmark
Tissue Antigens 63:395-400. 2004..These should be further validated in SARS survivors and used for vaccine formulation. We suggest that immunobioinformatics may become a fast and valuable tool in rational vaccine design... - Efficient assembly of recombinant major histocompatibility complex class I molecules with preformed disulfide bondsL Ostergaard Pedersen
Institute of Medical Microbiology and Immunology, University of Copenhagen, Copenhagen, Denmark
Eur J Immunol 31:2986-96. 2001..The analysis of the specificity of the several hundred human MHC haplotypes, should benefit considerably from the availability of pre-oxidized recombinant MHC-I... - Quantitative predictions of peptide binding to MHC class I molecules using specificity matrices and anchor-stratified calibrationsS L Lauemøller
Department of Experimental Immunology, Institute of Medical Microbiology and Immunology, University of Copenhagen, Copenhagen, Denmark
Tissue Antigens 57:405-14. 2001..This allowed us to define unique anchor-stratified calibrations, which led to predictions of improved precision and accuracy... - Modified human beta 2-microglobulin (desLys(58)) displays decreased affinity for the heavy chain of MHC class I and induces nitric oxide production and apoptosisM Wang
Department of International Health, Immunology and Microbiology, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark
Scand J Immunol 69:203-12. 2009.... - Longer peptide can be accommodated in the MHC class I binding site by a protrusion mechanismA Stryhn
Institute of Medical Microbiology and Immunology, University of Copenhagen, Copenhagen, Denmark
Eur J Immunol 30:3089-99. 2000..e. that extensions may be part of the specificity of the T cell immune response. We suggest that such extensions may play a physiological role... - Complete protection against lethal Toxoplasma gondii infection in mice immunized with a plasmid encoding the SAG1 geneH V Nielsen
Department of Mycobacteria and Parasitology, Statens Serum Institut, University of Copenhagen, Copenhagen, Denmark
Infect Immun 67:6358-63. 1999..In conclusion, DNA vaccination with p1tPASAG1 gave effective protection in mice against T. gondii infection and the protection could be adoptively transferred by purified CD8(+) T cells... - Phage display of peptide / major histocompatibility class I complexesN Vest Hansen
Institut of Medical Microbiology and Immunology, University of Copenhagen, Copenhagen, Denmark
Eur J Immunol 31:32-8. 2001..e. peptide-specific, MHC-I-restricted) antibody. Thus, properly assembled and folded peptide-MHC-I complexes can be displayed on filamentous phage. Despite the successful display, interaction with T cells could not be demonstrated... - Treatment of transplanted CT26 tumour with dendritic cell vaccine in combination with blockade of vascular endothelial growth factor receptor 2 and CTLA-4A E Pedersen
Laboratory of Cellular Immunology, Department of Medical Anatomy A, The Panum Institute, University of Copenhagen, Denmark
Cancer Lett 235:229-38. 2006..However, effective treatment of mice with already established tumours was only obtained after combination of vaccination, DC101 and 9H10 treatment in which setting 80% of the mice rejected their tumours... - Role of the T cell receptor ligand affinity in T cell activation by bacterial superantigensP S Andersen
Institute for Medical Microbiology and Immunology, University of Copenhagen, Blegdamsvej 3C, DK 2200 Copenhagen, Denmark
J Biol Chem 276:33452-7. 2001.... - Measurement of peptide-MHC interactions in solution using the spin column filtration assayS Buus
Institute of Medical Microbiology and Immunology, University of Copenhagen, Copenhagen, Denmark
Curr Protoc Immunol . 2001..It also demands fewer resources both in terms of unique reagents and labor, and it generates less hazardous waste. Thus, the spin column gel-filtration assay is ideal for routine work... - T cell responses affected by aminopeptidase N (CD13)-mediated trimming of major histocompatibility complex class II-bound peptidesS L Larsen
Department of Experimental Immunology, University of Copenhagen, Denmark
J Exp Med 184:183-9. 1996..This could be demonstrated both in cell-free systems using purified reagents and in cellular systems. Thus, MHC class II and APN may act in concert to generate the final T cell epitopes... - Self-peptides with intermediate capacity to bind and stabilize MHC class I molecules may be immunogenicM L M Andersen
Laboratory of Cellular Immunology, Department of Medical Anatomy, The Panum Institute, University of Copenhagen, Copenhagen, Denmark
Scand J Immunol 57:21-7. 2003..These data should be considered in the design of tumour vaccines based on MHC-I-binding self-peptides... - Description and prediction of peptide-MHC binding: the 'human MHC project'S Buus
Institute of Medical Microbiology and Immunology, Panum Building 18 3 22, Blegdamsvej 3, 2200, Copenhagen N, Denmark
Curr Opin Immunol 11:209-13. 1999..Recent advances in combinatorial peptide chemistry have improved the description and prediction of peptide-MHC binding. It is envisioned that a complete mapping of human immune reactivities will be possible... - The interaction of beta 2-microglobulin (beta 2m) with mouse class I major histocompatibility antigens and its ability to support peptide binding. A comparison of human and mouse beta 2mL O Pedersen
Institute of Medical Microbiology and Immunology, University of Copenhagen, Denmark
Eur J Immunol 25:1609-16. 1995..This would explain why mouse beta 2m is less efficient than human beta 2m in generating the peptide binding moiety, and identifies the empty MHC class I heavy chain as the molecule that binds human beta 2m preferentially... - Phenotypic and functional characterization of clinical grade dendritic cells generated from patients with advanced breast cancer for therapeutic vaccinationA E Pedersen
Laboratory of Cellular Immunology, Department of Medical Anatomy A, University of Copenhagen, Copenhagen, Denmark
Scand J Immunol 61:147-56. 2005..These data suggest that patient-derived DC are more differentiated but are less sensitive to maturation-inducing agents than DC obtained from healthy individuals... - Establishment of a quantitative ELISA capable of determining peptide - MHC class I interactionC Sylvester-Hvid
University of Copenhagen, Denmark
Tissue Antigens 59:251-8. 2002..This assay is simple and sensitive, and one can easily envisage that the necessary reagents, standards and protocols could be made generally available to the scientific community...