Targeted disruption of the alpha1,3-galactosyltransferase gene in cloned pigsYifan Dai
PPL Therapeutics Inc, 1700 Kraft Drive, Blacksburg, VA 24060, USA
Nat Biotechnol 20:251-5. 2002
..Using cells from one colony, we produced six cloned female piglets, of which five were of normal weight and apparently healthy. Southern blot analysis confirmed that these five piglets contain one disrupted pig alpha1,3GT allele...
- Production of alpha 1,3-galactosyltransferase-deficient pigs
Carol J Phelps
PPL Therapeutics Inc, 1700 Kraft Drive, Blacksburg, VA 24060, USA
Science 299:411-4. 2003
....
- Efficient production of transgenic cloned calves using preimplantation screening
Shu Hung Chen
PPL Therapeutics Inc, Blacksburg, Virginia 24060, USA
Biol Reprod 67:1488-92. 2002
..The results suggest that both selection methods are useful for detecting transgenic NT embryos without negatively affecting their development into viable transgenic offspring...
- Acute rejection is associated with antibodies to non-Gal antigens in baboons using Gal-knockout pig kidneys
Gang Chen
1] Department of Surgery, University of Western Ontario, London, Ontario N6A 5C1, Canada
Nat Med 11:1295-8. 2005
..These data suggest that antibodies to non-Gal antigens will present an additional barrier to transplantation of organs from GalT-KO pigs to humans...
- Generation of cloned transgenic pigs rich in omega-3 fatty acids
Liangxue Lai
Division of Animal Science, University of Missouri-Columbia, Columbia, Missouri 65211, USA
Nat Biotechnol 24:435-6. 2006
..The hfat-1 transgenic pigs produce high levels of n-3 fatty acids from n-6 analogs, and their tissues have a significantly reduced ratio of n-6/n-3 fatty acids (P < 0.001)...
- Cloned transgenic swine via in vitro production and cryopreservation
Rongfeng Li
Division of Animal Science, University of Missouri-Columbia, Columbia, Missouri 65211, USA
Biol Reprod 75:226-30. 2006
..Application of this technique may permit national and international movement of cloned transgenic swine embryos, storage until a suitable surrogate is available, or the long-term frozen storage of valuable genetics...
- Expression of the fat-1 gene diminishes prostate cancer growth in vivo through enhancing apoptosis and inhibiting GSK-3 beta phosphorylation
Yi Lu
Department of Medicine, University of Pittsburgh, VA Pittsburgh Healthcare Systems, Room 2E146, University Drive, Pittsburgh, PA 15240, USA
Mol Cancer Ther 7:3203-11. 2008
..These results suggest that fat-1 gene transfer directly into tumor cells could be used as a novel therapeutic approach...
