Research Topics
| M HolecekSummaryAffiliation: Charles University Country: Czech Republic Publications
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Publications
Alterations in protein metabolism and amino acid concentrations in rats fed by a high-protein (casein-enriched) diet - effect of starvationMilan Holecek
Department of Physiology, Charles University in Prague, Medical Faculty in Hradec Kralove, Czech Republic
Food Chem Toxicol 49:3336-42. 2011....- Acute hyperammonemia activates branched-chain amino acid catabolism and decreases their extracellular concentrations: different sensitivity of red and white muscleMilan Holecek
Department of Physiology, Medical Faculty in Hradec Kralove, Charles University in Prague, Simkova 870, 500 38, Hradec Kralove, Czech Republic
Amino Acids 40:575-84. 2011..These metabolic changes are not directly associated with marked changes in protein turnover. The effect of ammonia is more pronounced in muscles with high content of white fibres... - Three targets of branched-chain amino acid supplementation in the treatment of liver diseaseMilan Holecek
Department of Physiology, Charles University in Prague, Faculty of Medicine in Hradec Kralove, Czech Republic
Nutrition 26:482-90. 2010..An important task for clinical research is to identify groups of patients for whom BCAA treatment can significantly improve the health-related quality of life and the prognosis of hepatic disease... - Effect of beta-hydroxy-beta-methylbutyrate (HMB) on protein metabolism in whole body and in selected tissuesM Holecek
Department of Physiology and Biochemistry, Charles University, Medical Faculty, Hradec Kralove, Czech Republic
Food Chem Toxicol 47:255-9. 2009..Alterations in protein synthesis in visceral tissues may affect several important functions and the metabolic status of the whole body... - Simultaneous infusion of glutamine and branched-chain amino acids (BCAA) to septic rats does not have more favorable effect on protein synthesis in muscle, liver, and small intestine than separate infusionsMilan Holecek
Department of Physiology, Charles University, Medical Faculty, Hradec Kralove, Czech Republic
JPEN J Parenter Enteral Nutr 30:467-73. 2006..In the present study, we evaluated their effect on protein synthesis in skeletal muscle, liver, and jejunum of septic rats... - Proteasome inhibitor MG-132 enhances whole-body protein turnover in ratMilan Holecek
Department of Physiology, Charles University, Medical Faculty, Hradec Kralove, Czech Republic
Biochem Biophys Res Commun 345:38-42. 2006.... - [Glutamine and branched-chain amino acids--practical importance of their metabolic relations]M Holecek
Ustav fyziologie LF UK, Hradec Kralove
Cas Lek Cesk 144:9-12. 2005..Finally, it is suggested that the favourable effect of glutamine administration on protein metabolism is related to changes in BCAA metabolism... - Nutritional modulation of liver regeneration by carbohydrates, lipids, and amino acids: a reviewM Holecek
Department of Physiology, Charles University School of Medicine, Hradec Kralove, Czech Republic
Nutrition 15:784-8. 1999.... - Acute effects of acidosis on protein and amino acid metabolism in perfused rat liverMilan Holecek
Department of Physiology, Charles University Prague, Hradec Kralove, Czech Republic
Int J Exp Pathol 84:185-90. 2003.... - Effect of alanyl-glutamine on leucine and protein metabolism in irradiated ratsM Holecek
Department of Physiology, Charles University Medical Faculty, Hradec Kralove, Czech Republic
Amino Acids 22:95-108. 2002.... - Effect of alanyl-glutamine on leucine and protein metabolism in endotoxemic ratsM Holecek
Department of Physiology, Charles University, School of Medicine, Prague, Czech Republic
JPEN J Parenter Enteral Nutr 24:215-22. 2000..This study was performed to investigate whether exogenous alanine or glutamine would affect leucine and protein metabolism in intact and endotoxemic rats... - Leucine and protein metabolism in rats with chronic renal insufficiencyM Holecek
Department of Physiology, Charles University School of Medicine, Hradec Kralove, Czech Republic
Exp Toxicol Pathol 53:71-6. 2001..We conclude that marked activation of proteolysis occurs in severe chronic renal failure and is probably caused by metabolic changes related to acidosis development... - Influence of buthionine sulfoximine, S-adenosylmethionine and glutathione on liver regeneration following partial hepatectomyM Holecek
Charles University School of Medicine, Department of Physiology and Radioisotope Laboratory, Hradec Kralove, Czech Republic
Arzneimittelforschung 50:1093-8. 2000..It is concluded that derangement in methionine metabolism can impair the development of liver regeneration. The data also demonstrate the favourable effect of SAM and negative effect of GSH... - Metabolism of branched-chain amino acids in starved rats: the role of hepatic tissueM Holecek
Department of Physiology, Faculty of Medicine, Charles University, Hradec Krcálové, Czech Republic
Physiol Res 50:25-33. 2001..The increase in plasma BCAA levels in starved animals results in part from decreased BCAA catabolism, particularly in heart and skeletal muscles, and from a net release of BCAA by the hepatic tissue... - Effect of starvation on branched-chain alpha-keto acid dehydrogenase activity in rat heart and skeletal muscleM Holecek
Department of Physiology, Faculty of Medicine, Charles University, Hradec Kralove, Czech Republic
Physiol Res 50:19-24. 2001..The increased activity of BCKAD on the 6th day of starvation indicates activated oxidation of BCAA and accelerated protein breakdown... - Leucine and protein metabolism after bilateral nephrectomy in rats: the role of hepatic tissueM Holecek
Department of Physiology, Charles University School of Medicine, Hradec Kralove, Czech Republic
Res Exp Med (Berl) 200:53-65. 2000..The data obtained in the IPL model indicate that BNX causes metabolic changes that enable resynthesis of BCAA from corresponding branched-chain keto acids in liver... - The effect of new proteasome inhibitors, belactosin A and C, on protein metabolism in isolated rat skeletal muscleT Muthny
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Charles University, Hradec Kralove, Czech Republic
J Physiol Biochem 65:137-46. 2009..In summary, belactosin A and C affected basic parameters of protein metabolism in rat skeletal muscle. The response was both muscle- and belactosin-type-dependent... - Acute effects of decreased glutamine supply on protein and amino acid metabolism in hepatic tissue: a study using isolated perfused rat liverMilan Holecek
Department of Physiology, Charles Unviersity Prague, Medical Faculty, Hradec Kralove, Czech Republic
Metabolism 52:1062-7. 2003.... - Protein metabolism in guanethidine-treated ratsMilan Holecek
Department of Physiology, Charles University School of Medicine, , Czech Republic
Int J Exp Pathol 85:257-64. 2004..These changes may also be induced by administration of other sympathetic blocking agents, e.g. in treatment of hypertension... - Direct effects of proteasome inhibitor AdaAhx3L3VS on protein and amino acid metabolism in rat skeletal muscleJ Kadlcikova
Department of Pharmacology, Faculty of Pharmacy, Charles University, Hradec Kralove, Czech Republic
Physiol Res 54:541-7. 2005..The release of amino acids into the medium was reduced. We conclude that AdaAhx3L3VS significantly decreased proteolysis and protein synthesis and increased leucine oxidation... - Effect of a keto acid-amino acid supplement on the metabolism and renal elimination of branched-chain amino acids in patients with chronic renal insufficiency on a low protein dietV Teplan
Department of Nephrology, Transplant Center, Institute of Clinical and Experimental Medicine, Postgraduate Medical School, Prague, Czech Republic
Wien Klin Wochenschr 112:876-81. 2000.... - Modulation of inflammatory response in sepsis by proteasome inhibitionRoman Safranek
Department of Surgery, School of Medicine, Kurume University, Kurume, Japan
Int J Exp Pathol 87:369-72. 2006..IL-6 was not significantly affected. A mortality study revealed prolonged survival in MG-132 treated mice. We conclude that MG-132 treatment decreases inflammatory response and prolongs survival in the CLP model of sepsis... - Protein metabolism in slow- and fast-twitch skeletal muscle during turpentine-induced inflammationTomas Muthny
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Charles University, Hradec Kralove, Czech Republic
Int J Exp Pathol 89:64-71. 2008..Release of leucine was inhibited significantly more in SOL. We conclude that turpentine-induced inflammation affects more CHTLA, protein synthesis and leucine release in EDL compared to SOL... - Effects of proteasome inhibitors MG132, ZL3VS and AdaAhx3L3VS on protein metabolism in septic ratsJana Kadlcikova
Department of Pharmacology, Faculty of Pharmacy, Charles University, Heyrovského 1230, 500 05 Hradec Kralove, Czech Republic
Int J Exp Pathol 85:365-71. 2004..Leucine oxidation was increased by AdaAhx(3)L(3)VS in the septic EDL muscle and decreased by MG132 in intact EDL muscle. We conclude that MG132 and AdaAhx(3)L(3)VS reversed protein catabolism in septic rat muscles... - Relation between glutamine, branched-chain amino acids, and protein metabolismMilan Holecek
Department of Physiology, Charles University School of Medicine, Hradec Kralove, Czech Republic
Nutrition 18:130-3. 2002..In conclusion some favorable effects of BCAA supply can be explained by its role in the synthesis of glutamine and some positive effects of glutamine exogenous supply can be explained by its effect on metabolism of BCAA... - Aspects of protein and amino acid metabolism in a model of severe glutamine deficiency in sepsisRoman Safranek
Department of Physiology, Faculty of Medicine, Charles University, Hradec Kralove, Czech Republic
Ann Nutr Metab 50:361-7. 2006..The aim was to investigate the effects of glutamine deficiency in sepsis with and without GH treatment on protein and amino acid metabolism...