Research Topics
Genomes and GenesSpecies | P C ShawSummaryAffiliation: The Chinese University of Hong Kong Country: China Publications
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Detail Information
Publications
Location and reduction of icarapin antigenicity by site specific coupling to polyethylene glycolKa Lok Wong
State Key Laboratory of Phytochemistry and Plant Resources in West China CUHK, Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, N T, Hong Kong, China
Protein Pept Lett 19:238-43. 2012..Our work may be generalized to other proteins for the discovery of antigenic sites and the reduction of antigenicity...- An integrated web medicinal materials DNA database: MMDBD (Medicinal Materials DNA Barcode Database)Shao Ke Lou
Department of Biochemistry, The Chinese University of Hong Kong, Shatin, Hong Kong, China
BMC Genomics 11:402. 2010..Owing to the reduced cost in DNA authentication, the volume of the DNA barcodes produced for medicinal materials is on the rise and necessitates the development of an integrated DNA database... - Isolation and chromosomal mapping of human glycogen synthase kinase-3 alpha and -3 beta encoding genesP C Shaw
Department of Biochemistry, Chinese University of Hong Kong, Shatin, NT, Hong Kong
Genome 41:720-7. 1998..2. On the other hand, by somatic cell hybrid panel DNA amplification and fluorescence in situ hybridisation using the 285-kb YAC clone, GSK-3 beta was mapped to 3q13.3... - Patent applications for using DNA technologies to authenticate medicinal herbal materialPang Chui Shaw
Department of Biochemistry, Chinese University of Hong Kong, Shatin, Hong Kong, PR China
Chin Med 4:21. 2009..It also briefly explains why these patents have been granted... - Recent advances in trichosanthin, a ribosome-inactivating protein with multiple pharmacological propertiesPang Chui Shaw
Department of Biochemistry, Centre for Protein Science and Crystallography, The Chinese University of Hong Kong, Shatin, N T, Hong Kong, China
Toxicon 45:683-9. 2005..These findings give new insights on the pharmacological properties of TCS and other members of ribosome-inactivating proteins... - Structural basis for the interaction of [E160A-E189A]-trichosanthin with adeninePang Chui Shaw
Department of Biochemistry, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, People s Republic of China
Toxicon 41:575-81. 2003.... - Forensically informative nucleotide sequencing (FINS) for the authentication of Chinese medicinal materialsMing Li
State Key Laboratory of Phytochemistry and Plant Resources in West China CUHK, Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China
Chin Med 6:42. 2011..Success of the FINS identification depends on the selection of DNA region and reference species. This article describes the techniques and applications of FINS for authenticating Chinese medicinal materials... - Expression analysis of glycogen synthase kinase-3 in human tissuesK F Lau
Department of Biochemistry, The Chinese University of Hong Kong, Shatin
J Pept Res 54:85-91. 1999.... - Molecular cloning and characterization of the human glycogen synthase kinase-3beta promoterK F Lau
Department of Biochemistry, The Chinese University of Hong Kong, Shatin, N T, Hong Kong, China
Genomics 60:121-8. 1999..CP2 has recently been shown to interact with the Alzheimer disease amyloid precursor protein binding protein Fe65. The significance of these results with respect to Alzheimer disease pathogenesis are discussed... - Application of sequence characterized amplified region (SCAR) analysis to authenticate Panax species and their adulterantsJ Wang
Department of Biochemistry, The Chinese University of Hong Kong, Shatin, Hong Kong, China
Planta Med 67:781-3. 2001..quinquefolius and its homolog in P. ginseng is the presence of a 25 bp insertion in the latter. Primers derived from this sequence were successfully used to authenticate six Panax species and two common adulterants... - Authentication of medicinal Dendrobium species by the internal transcribed spacer of ribosomal DNAD T Lau
Department of Biology, The Chinese University of Hong Kong, Shatin
Planta Med 67:456-60. 2001..Therefore, ITS 2 regions could be adopted as a molecular marker for differentiating medicinal Dendrobium species from one another and also from non-orchids and adulterants... - Trichosanthin interacts with acidic ribosomal proteins P0 and P1 and mitotic checkpoint protein MAD2BS H Chan
Department of Biochemistry, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
Eur J Biochem 268:2107-12. 2001..Our present study suggests that TCS may disrupt the binding of elongation factors to the P-complex, in addition to the well-known N-glycosidase activity for ribosome inactivation... - Anti-acetylcholinesterase activities of traditional Chinese medicine for treating Alzheimer's diseaseH Q Lin
Department of Biochemistry, The Chinese University of Hong Kong, Shatin, N T, Hong Kong, SAR, China
Chem Biol Interact 175:352-4. 2008..The IC(50) of these herbal extracts on inhibition of AChE are at around 5-85 microm/ml. The results of this study indicate that there is a great potential to search for novel usage of these medicinal herbs for the treatment of AD... - Authentication of Panax ginseng and Panax quinquefolius using amplified fragment length polymorphism (AFLP) and directed amplification of minisatellite region DNA (DAMD)W Y Ha
Department of Biochemistry and Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, China
J Agric Food Chem 50:1871-5. 2002..The minisatellite DNA was consequently used in directed amplification minisatellite region DNA analysis to authenticate the two ginsengs... - Interaction between trichosanthin, a ribosome-inactivating protein, and the ribosomal stalk protein P2 by chemical shift perturbation and mutagenesis analysesDenise S B Chan
Department of Biochemistry, Centre for Protein Science and Crystallography and Molecular Biotechnology Programme, The Chinese University of Hong Kong, Shatin, Hong Kong, China
Nucleic Acids Res 35:1660-72. 2007..In an analogy to the role of stalk proteins in binding elongation factors, we propose that interaction with acidic ribosomal stalk proteins help TCS to locate its RNA substrate... - Structure-function study of maize ribosome-inactivating protein: implications for the internal inactivation region and the sole glutamate in the active siteAmanda Nga Sze Mak
Department of Biochemistry, Centre for Protein Science and Crystallography, The Chinese University of Hong Kong, Shatin, N T, Hong Kong, China
Nucleic Acids Res 35:6259-67. 2007..Our structure suggests that maize ribosome-inactivating protein may represent an intermediate product in the evolution of ribosome-inactivating proteins... - Recent progress in medicinal investigations on trichosanthin and other ribosome inactivating proteins from the plant genus TrichosanthesE F Fang
School of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, Hong Kong, China
Curr Med Chem 18:4410-7. 2011..Regarding the debilitating nature of AIDS and different tumors, further understanding of these multifunctional proteins is worthwhile since it may help to open a novel therapeutic window for these stubborn diseases... - Trichosanthin interacts with and enters cells via LDL receptor family membersW L Chan
Department of Physiology, Department of Biochemistry, Chinese University of Hong Kong, Shatin, N T, Hong Kong SAR, China
Biochem Biophys Res Commun 270:453-7. 2000..The results suggest that the known abortifacient and renotoxic actions of trichosanthin are caused by LRP-mediated uptake in trophoblasts and megalin-mediated uptake in proximal tubule epithelial cells, respectively... - Structure of the influenza virus A H5N1 nucleoprotein: implications for RNA binding, oligomerization, and vaccine designAndy Ka Leung Ng
Department of Biochemistry and Center for Protein Science and Crystallography, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China
FASEB J 22:3638-47. 2008..Our study of H5N1 NP provides insight into the oligomerization interface and the RNA-binding groove, which are attractive drug targets, and it identifies the epitopes that might be used for universal vaccine development... - Characterization of the large subunit of EcoHK31I methyltransferase by structural modeling and mutagenesisAmanda N S Mak
Department of Biochemistry and Center for Protein Science and Crystallography, The Chinese University of Hong Kong, Shatin, Hong Kong, China
Biol Chem 388:265-71. 2007..Activity assays indicated that most of these charges can be eliminated or changed conservatively. Among these charged residues, R224, E240, D245 and D251 may take part in proper interaction with DNA in the presence of polypeptide beta... - Lignans from Dysosma versipellis with inhibitory effects on prostate cancer cell linesRen Wang Jiang
Institute of Chinese Medicine, Department of Biochemistry and Department of Biology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, People s Republic of China
J Nat Prod 70:283-6. 2007..Thus, cultivation of D. versipellis in suitable locations may serve as an alternative source for podophyllotoxin (2) production... - Alkaloids and chemical diversity of Stemona tuberosaRen-Wang Jiang
Institute of Chinese Medicine, Department of Biology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, People's Republic of China
J Nat Prod 69:749-54. 2006..tuberosa from 13 localities, and four types of chemical variation featured by the major components 3, neotuberostemonine (4), croomine (5), and stemoninine (6), respectively, were observed... - Expression of glycogen synthase kinase-3 isoforms in mouse tissues and their transcription in the brainHong bing Yao
Department of Biochemistry, The Chinese University of Hong Kong, Shatin, N T, Hong Kong, China
J Chem Neuroanat 23:291-7. 2002..The differential expression of GSK-3alpha and -3beta mRNAs as well as proteins in other tissues indicate that they play different roles in cellular functions and the developmental process... - Substrate binding and catalysis in trichosanthin occur in different sites as revealed by the complex structures of several E85 mutantsQing Guo
Laboratory of Structural Biology and the MOE Laboratory of Protein Science, School of Life Science and Engineering, Tsinghua University, Beijing 100084, China
Protein Eng 16:391-6. 2003..Based on these complex structures, the function of residue 85 and the mechanism of catalysis are proposed... - The C-terminal end of P proteins mediates ribosome inactivation by trichosanthin but does not affect the pokeweed antiviral protein activityMaximiliano Juri Ayub
Fundacion Instituto Leloir, IIBBA CONICET and University of Buenos Aires, Patricias Argentinas 435, 1405 Buenos Aires, Argentina
Biochem Biophys Res Commun 369:314-9. 2008..cruzi ribosomes, and most importantly, does not require from the C-terminal end of P proteins. These results could explain, at least partially, the different selectivity of these toxins against prokaryotic and eukaryotic ribosomes... - Trypanosoma cruzi: high ribosomal resistance to trichosanthin inactivationMaximiliano Juri Ayub
Fundacion Instituto Leloir, IIBBA CONICET, Avenida Patricias Argentinas 435, 1405 Buenos Aires, Argentina
Exp Parasitol 118:442-7. 2008..This mutant showed similar shifting of IC(50) values on rat, T. cruzi and C. fasciculata ribosomes, suggesting that the resistance mechanism might involve other ribosomal components rather than the C-terminal end of P proteins... - Crystal structure of mastoparan from Polistes jadwagae at 1.2 A resolutionShengquan Liu
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101, China
J Struct Biol 160:28-34. 2007..Together with biochemical results, we propose that the interactions between mastoparan molecules play an important role in forming the alpha-helical conformation, which is highly related to their biological activities... - Glycogen synthase kinase-3 beta-mediated tau phosphorylation in cultured cell linesChris Wing-Cheung Lee
Department of Biochemistry, The Chinese University of Hong Kong, Shatin, China
Neuroreport 14:257-60. 2003..This suggests a novel mechanism whereby lithium-mediated inhibition of GSK-3beta activity influences tau phosphorylation... - A phylogenetic analysis of Epimedium (Berberidaceae) based on nuclear ribosomal DNA sequencesYe Sun
Department of Biochemistry, The Chinese University of Hong Kong, Shatin, N.T, Hong Kong, China
Mol Phylogenet Evol 35:287-91. 2005 - Characterization of medicinal Epimedium species by 5S rRNA gene spacer sequencingYe Sun
Department of Biochemistry, The Chinese University of Hong Kong, Shatin, China
Planta Med 70:287-8. 2004..pubescens, E. wushanense, and E. brevicornu. A 19-bp deletion was found for E. koreanum in the 5S rRNA gene spacer. E. koreanum was most divergent from the other four endemic Chinese species of Epimedium... - Trichosanthin induces leakage and membrane fusion of liposomeXiao-Feng Xia
Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, People's Republic of China
IUBMB Life 55:681-7. 2003..Deleting the C-terminus almost completely abolishes the destabilizing effect of TCS on the phospholipid bilayer, which implicates the C-terminus in the interaction between trichosanthin and the membrane... - A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genesRichard J Roberts
New England Biolabs, Beverly, MA 01915, USA
Nucleic Acids Res 31:1805-12. 2003..It provides explicit categories for the many different Type II enzymes now identified and provides a system for naming the putative genes found by sequence analysis of microbial genomes...