Blocking transforming growth factor-beta receptor signaling down-regulates transforming growth factor-beta1 autoproduction in keloid fibroblastsWei Liu
Department of Plastic and Reconstructive Surgery, Shanghai Institute of Plastic and Reconstructive Surgery, Tissue Engineering Center, Shanghai 9th People s Hospital, Shanghai Second Medical University, Shanghai 200011, China
Chin J Traumatol 5:77-81. 2002
..To study transforming growth factor-beta1 (TGF-beta1) autoproduction in keloid fibroblasts and the regulation effect of blocking TGF-beta intracellular signaling on rhTGF-beta1 autoproduction...
- Donor age and cell passage affects differentiation potential of murine bone marrow-derived stem cells
James D Kretlow
Department of Bioengineering, Rice University, PO Box 1892, MS 142, Houston, TX 77251 1892, USA
BMC Cell Biol 9:60. 2008
..Furthermore, there currently exists little understanding as to how these two factors may be influenced by one another...
- Tissue engineering and tissue repair in immunocompetent animals: tissue construction and repair
Y Cao
Department of Plastic and Reconstructive Surgery, Shanghai 9th People s Hospital, National Tissue Engineering Center of China, Shanghai Jiao Tong University School of Medicine, Shanghai, People s Republic of China
Handchir Mikrochir Plast Chir 39:156-60. 2007
..As an important step towards a clinical application, research work in large animals is essential. This article presents our experience of tissue engineering and repair in large animals in the areas of bone, cartilage and tendon...
- Bridging tendon defects using autologous tenocyte engineered tendon in a hen model
Yilin Cao
Department of Plastic and Reconstructive Surgery, Shanghai Ninth People s Hospital, Tissue Engineering Center, Shanghai Second Medical University, Shanghai, ROC
Plast Reconstr Surg 110:1280-9. 2002
..The engineered tendons resembled natural tendons not only in gross appearance and histologic structure but also in biomechanical properties...
- The role of bFGF in down-regulating ?-SMA expression of chondrogenically induced BMSCs and preventing the shrinkage of BMSC engineered cartilage
Qiong Li
Department of Plastic and Reconstructive Surgery, Shanghai 9th People s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, Shanghai Stem Cell Institute, 639 Zhi Zao Ju Road, Shanghai 200011, PR China
Biomaterials 32:4773-81. 2011
....
- In vitro precultivation alleviates post-implantation inflammation and enhances development of tissue-engineered tubular cartilage
Xusong Luo
Department of Plastic and Reconstructive Surgery, Shanghai Key Laboratory of Tissue Engineering, Shanghai 9th People s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, People s Republic of China
Biomed Mater 4:025006. 2009
..These results demonstrated that the post-implantation inflammatory reaction could be alleviated by in vitro precultivation, which contributes to the formation of satisfactory tubular cartilage for tracheal reconstruction...
- Repair of articular cartilage defect in non-weight bearing areas using adipose derived stem cells loaded polyglycolic acid mesh
Lei Cui
Department of Plastic and Reconstructive Surgery, Shanghai 9th People s Hospital, Shanghai Jiao Tong University School of Medicine, 639 Zhi Zao Ju Road, Shanghai 200011, PR China
Biomaterials 30:2683-93. 2009
..55% of the normal cartilage at 3 months to 88.05% at 6 months. The successful repair thus substantiates the potentiality of using chondrogenic induced ASCs and PGA/PLA scaffold for cartilage regeneration...
- In vivo ectopic chondrogenesis of BMSCs directed by mature chondrocytes
Xia Liu
Department of Plastic and Reconstructive Surgery, Shanghai 9th People s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, 639 Zhi Zao Ju Road, Shanghai 200011, PR China
Biomaterials 31:9406-14. 2010
....
- In vivo engineering of a functional tendon sheath in a hen model
Liang Xu
Department of Plastic and Reconstructive Surgery, Shanghai 9th People s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, PR China
Biomaterials 31:3894-902. 2010
..Taken together, these results suggest that tendon sheaths that are functionally and structurally similar to native sheaths are possible to be engineered in vivo using tendon sheath cells and PGA scaffolds...
- The dependence of in vivo stable ectopic chondrogenesis by human mesenchymal stem cells on chondrogenic differentiation in vitro
Kai Liu
Department of Plastic and Reconstructive Surgery, Shanghai 9th People s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, 639 Zhi Zao Ju Road, Shanghai 200011, PR China
Biomaterials 29:2183-92. 2008
..These results indicate that a fully differentiated stage achieved by extended chondrogenic induction in vitro is necessary for hBMSCs to form stable ectopic chondrogenesis in vivo...
- A small diameter elastic blood vessel wall prepared under pulsatile conditions from polyglycolic acid mesh and smooth muscle cells differentiated from adipose-derived stem cells
Chen Wang
Department of Plastic and Reconstructive Surgery, Shanghai 9th People s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, PR China
Biomaterials 31:621-30. 2010
....
- The role of the extracellular signal-related kinase signaling pathway in osteogenic differentiation of human adipose-derived stem cells and in adipogenic transition initiated by dexamethasone
Qihai Liu
Department of Plastic and Reconstructive Surgery, Shanghai 9th People s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People s Republic of China
Tissue Eng Part A 15:3487-97. 2009
....
- In vitro engineering of fibrocartilage using CDMP1 induced dermal fibroblasts and polyglycolide
Guiqing Zhao
Department of Plastic and Reconstructive Surgery, Shanghai 9th People s Hospital, Shanghai Jiao Tong University School of Medicine, 639 Zhi Zao Ju Road, Shanghai 200011, PR China
Biomaterials 30:3241-50. 2009
..Furthermore, the potential of using DFs as seed cells to engineer fibrocartilage is substantiated and further study on using the engineered tissue to repair fibrocartilage defects is currently ongoing in our group...
- Differentiation of adipose-derived stem cells into contractile smooth muscle cells induced by transforming growth factor-beta1 and bone morphogenetic protein-4
Chen Wang
Department of Plastic and Reconstructive Surgery, Shanghai Jiao Tong University School of Medicine, Shanghai, People s Republic of China
Tissue Eng Part A 16:1201-13. 2010
..SMCs differentiated from hASCs may provide an abundant source as seed cells for blood vessel engineering...
- In vitro engineering of human ear-shaped cartilage assisted with CAD/CAM technology
Yu Liu
Department of Plastic and Reconstructive Surgery, Shanghai 9th People s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, 639 Zhi Zao Ju Road, Shanghai 200011, PR China
Biomaterials 31:2176-83. 2010
..These results may provide a useful strategy for reconstructing cartilage tissue with complicated shapes such as a human ear by an in vitro engineering approach...
- Mesenchymal-like stem cells derived from human parthenogenetic embryonic stem cells
Yao Chen
Shanghai Key Laboratory of Tissue Engineering, Department of Plastic and Reconstructive Surgery, Shanghai 9th People s Hospital, Shanghai Jiao Tong University School of Medicine, National Tissue Engineering Center of China, Shanghai, China
Stem Cells Dev 21:143-51. 2012
..This is the first work that demonstrates the differentiation of hpESCs into multipotent MSC-like cells. These hpESCs could be a potential source for cell-based therapies...
- Chondrogenic transdifferentiation of human dermal fibroblasts stimulated with cartilage-derived morphogenetic protein 1
Shuo Yin
Department of Plastic and Reconstructive Surgery, Shanghai 9th People s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Tissue Eng Part A 16:1633-43. 2010
..The integrin alpha5 mediating cell-cell and cell-matrix interactions is required for maintaining the chondrogenic phenotype of DFs...
- Engineering human neo-tendon tissue in vitro with human dermal fibroblasts under static mechanical strain
Dan Deng
Department of Plastic and Reconstructive Surgery, Shanghai Ninth People s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, 639 Zhi Zao Ju Road, Shanghai 200011, PR China
Biomaterials 30:6724-30. 2009
....
- Repair of porcine articular osteochondral defects in non-weightbearing areas with autologous bone marrow stromal cells
Guangdong Zhou
Department of Plastic and Reconstructive Surgery, 9th People's Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, PR China
Tissue Eng 12:3209-21. 2006
..These results strongly indicate that the implanted BMSCs can differentiate into either chondrocytes or osteoblasts and repair articular osteochondral defects by forming engineered cartilage and engineered bone...
- In vitro and in vivo evaluation of osteogenesis of human umbilical cord blood-derived mesenchymal stem cells on partially demineralized bone matrix
Guangpeng Liu
The Key Laboratory of Tissue Engineering, Shanghai 9th People s Hospital, Shanghai JiaoTong Universtiy School of Medicine, Shanghai, China
Tissue Eng Part A 16:971-82. 2010
..These results demonstrate that pDBM can support osteogenic differentiation of human UCB-MSCs in vitro and in vivo, and UCB-MSCs may serve as an alternative cell source for bone tissue engineering and regeneration...
- Tissue engineering of corneal stromal layer with dermal fibroblasts: phenotypic and functional switch of differentiated cells in cornea
Yan Qing Zhang
Shanghai Jiao Tong University School of Medicine, Plastic and Reconstructive Surgery, Shanghai 9th People s Hospital, Shanghai, China
Tissue Eng Part A 14:295-303. 2008
..The functional restoration of corneal transparency using dermal fibroblasts suggests that they could be an alternative cell source for corneal stroma engineering...
- Potent in vitro chondrogenesis of CD105 enriched human adipose-derived stem cells
Ting Jiang
Department of Plastic and Reconstructive Surgery, Shanghai 9th People s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, 639 Zhi Zao Ju Road, Shanghai 200011, PR China
Biomaterials 31:3564-71. 2010
....
- Differentiation of human multipotent dermal fibroblasts into islet-like cell clusters
Dan Bi
Department of Plastic and Reconstructive Surgery, Shanghai 9th People s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, National Tissue Engineering Center of China, Shanghai 200011, China
BMC Cell Biol 11:46. 2010
..It is of great interest to know whether these cells could be further differentiated into functional insulin-producing cells...
- Remodeling of buccal mucosa by bladder microenvironment
Mujun Lu
Department of Plastic and Reconstructive Surgery, Shanghai 9th People s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, Shanghai, PR China
Urology 75:1514.e7-14. 2010
..This study explored the feasibility of phenotype transformation of oral mucosa towards bladder urothelium by transplanting the oral mucosa into bladder local microenvironment...
- Compressed collagen gel as the scaffold for skin engineering
Kuikui Hu
Department of Plastic and Reconstructive Surgery, Shanghai 9th People s Hospital, Shanghai Jiao Tong University School of Medicine, 639 Zhi Zao Ju Rd, Shanghai, 200011, People s Republic of China
Biomed Microdevices 12:627-35. 2010
..This study reveals the potential of using compressed collagen gel as a scaffold for skin engineering...
- Tissue engineering of cartilage, tendon and bone
Hengyun Sun
Department of Plastic and Reconstructive Surgery, Shanghai 9th People s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, Shanghai, 200011, China
Front Med 5:61-9. 2011
..Based on sophisticated animal studies, several clinical trials of engineered bone have been launched with promising preliminary results, displaying the high potential for clinical application...
- Chondrogenic differentiation of mouse embryonic stem cells promoted by mature chondrocytes
Feng Xie
Department of Plastic and Reconstructive Surgery, Shanghai 9th People s Hospital, Shanghai, 200011, China
Sci China C Life Sci 51:774-8. 2008
..Positive staining of mouse Major Histocompatibility Complex class I molecules confirmed that part of the chondrocytes were derived from mouse ES cells. The current study established a new approach for directing ES cell differentiation...
- [Experiment of adipose derived stem cells induced into smooth muscle cells]
Ping Yang
Department of Plastic and Reconstructive Surgery, Ninth People s Hospital, Affiliated to Shanghai Jiaotong University, Shanghai, 200011, P R China
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 22:481-6. 2008
..To study the feasibility of human adipose derived stem cells (ADSCs) in monolayer culture induced into smooth muscle cells in vitro as seeding cells in vascular tissue engineering...
- Repair of cranial bone defects with adipose derived stem cells and coral scaffold in a canine model
Lei Cui
Department of Plastic and Reconstructive Surgery, Shanghai Ninth People s Hospital, Shanghai Stem Cell Institute, Shanghai Jiao Tong University School of Medicine, 639 Zhi Zao Ju Road, Shanghai 200011, PR China
Biomaterials 28:5477-86. 2007
..The successful repair of critical-sized bone defects via the current approach substantiates the potentiality of using ASCs with coral scaffolds for bone regeneration...
- In vitro formation of lacuna structure by human dermal fibroblasts co-cultured with porcine chondrocytes on a 3D biodegradable scaffold
Xia Liu
Department of Plastic and Reconstructive Surgery, Shanghai 9th People s Hospital, Shanghai Jiao Tong University School of Medicine, 200011, Shanghai, China
Biotechnol Lett 29:1685-90. 2007
..Moreover, the 3D-co-culture system can serve as an optimal model for directing stem cell differentiation in vitro...
- Platelet endothelial cell adhesion molecule-1, stage-specific embryonic antigen-1, and Flk-1 mark distinct populations of mouse embryonic stem cells during differentiation toward hematopoietic/endothelial cells
Wei Yue
Department of Plastic and Reconstructive Surgery, National Tissue Engineering Center of China, Shanghai Jiao Tong University School of Medicine, Shanghai 9th People s Hospital, Shanghai, China
Stem Cells Dev 19:1937-48. 2010
....
- Adenoviral transduction of hTGF-beta1 enhances the chondrogenesis of bone marrow derived stromal cells
Wanyao Xia
Department of Plastic and Reconstructive Surgery, Shanghai Ninth People s Hospital, Shanghai Tissue Engineering Center, Shanghai Jiao Tong University School of Medicine, 639 Zhizaoju Road, Shanghai, 200011, People s Republic of China
Biotechnol Lett 31:639-46. 2009
..The results demonstrate that transfer of hTGF-beta1 into BMSCs via adenoviral transduction can induce chondrogenic differentiation in vitro and enhance chondrogenesis in vivo...
- Enhanced proliferation capacity of porcine tenocytes in low O2 tension culture
Yanjie Zhang
Department of Plastic and Reconstructive Surgery, Shanghai 9th People s Hospital, Shanghai Key Laboratory of Tissue Engineering Research, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, People s Republic of China
Biotechnol Lett 32:181-7. 2010
..In conclusion, low O(2) tension culture can significantly enhance the expansion capacity of tenocytes without affecting their phenotype and functions...
- Cryopreservation of tissue-engineered epithelial sheets in trehalose
FanFan Chen
Department of Plastic and Reconstructive Surgery, Shanghai 9th People s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, 639 Zhi Zao Ju Road, Shanghai 200011, PR China
Biomaterials 32:8426-35. 2011
..The results indicate that the use of trehalose improves cryopreservation of tissue-engineered epithelial sheets...
- [Construction of recombinant human smad 7 adenoviral vector and its expression in vitro]
Danru Wang
Department of Plastic and Reconstructive Surgery, Shanghai Ninth People s Hospital, Shanghai Second Medical University, Shanghai, China
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 22:769-72. 2005
..These results demonstrated that the recombinant Smad7 adenoviral vector can be expressed in cultured cells in vitro, and it may provide a new therapeutic strategy for keloid gene therapy...
- Tissue engineering of cartilage with the use of chitosan-gelatin complex scaffolds
Wanyao Xia
Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Second Medical University, People's Republic of China
J Biomed Mater Res B Appl Biomater 71:373-80. 2004
..Thus, this study demonstrated that the chitosan-gelatin complex may serve as a suitable scaffold for cartilage tissue engineering...
- Repair of tendon defect with dermal fibroblast engineered tendon in a porcine model
Wei Liu
Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Tissue Engineering Center, Shanghai Second Medical University, Shanghai, PR China
Tissue Eng 12:775-88. 2006
..05). These results suggest that dermal fibroblasts may have the potential as seed cells for tendon engineering...
- Engineering of an elastic large muscular vessel wall with pulsatile stimulation in bioreactor
Zhi C Xu
Department of Plastic and Reconstructive Surgery, Shanghai 9th People s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, Shanghai, China
Biomaterials 29:1464-72. 2008
..Our results indicate that the approach developed in the current work is efficient for large vessel engineering. This approach may also be suitable for the engineering of other tissues with muscular tubular structure...
- Repair of canine mandibular bone defects with bone marrow stromal cells and porous beta-tricalcium phosphate
Jie Yuan
Shanghai Key Laboratory of Tissue Engineering, Department of Plastic and Reconstructive Surgery, Shanghai 9th People s Hospital, Shanghai Jiao Tong University School of Medicine, 639 Zhi Zao Ju Road, Shanghai 200011, China
Biomaterials 28:1005-13. 2007
..05). Based on these results, we conclude that engineered bone from osteogenically induced BMSCs and biodegradable beta-TCP can well repair the critical-sized segmental mandibular defects in canines...
- The impact of low levels of collagen IX and pyridinoline on the mechanical properties of in vitro engineered cartilage
Dan Yan
Department of Plastic and Reconstructive Surgery, Shanghai 9th People s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, 639 Zhi Zao Ju Road, Shanghai 200011, PR China
Biomaterials 30:814-21. 2009
..Thus, the discovery provides a clue for engineering strong cartilage in vitro in the future by enhancing the levels of these two molecules...
- In vitro tendon engineering with avian tenocytes and polyglycolic acids: a preliminary report
Dejun Cao
Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, China
Tissue Eng 12:1369-77. 2006
..Thus, application of dynamic mechanical load with a bioreactor to the construction of tendon tissue will be our next goal in this series of in vitro tendon engineering study...
- Clonal analysis of nestin(-) vimentin(+) multipotent fibroblasts isolated from human dermis
Fu Guo Chen
Department of Plastic and Reconstructive Surgery, Shanghai 9th People s Hospital, Shanghai Stem Cell Institute, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, Shanghai 200011, China
J Cell Sci 120:2875-83. 2007
....
- Repair of experimental alveolar bone defects by tissue-engineered bone
Yulai Weng
Department of Plastic and Reconstructive Surgery, 9th People's Hospital, Shanghai Second Medical University, Shanghai, P.R. China
Tissue Eng 12:1503-13. 2006
..01). We thus conclude that BMSCs can be induced to become osteogenic and can be used as seed cells to engineer bone tissue and repair experimental alveolar bone defects...
- Degradation or excretion of quantum dots in mouse embryonic stem cells
Qing Meng Pi
Department of Plastic and Reconstructive Surgery, Shanghai 9th People s Hospital, Shanghai Jiao Tong University School of Medicine, National Tissue Engineering Center of China, Shanghai, China
BMC Biotechnol 10:36. 2010
..The purpose of this study is to investigate the metabolism of QDs in different type of cells...
- Collagen tissue engineering: development of novel biomaterials and applications
Lian Cen
Department of Plastic and Reconstructive Surgery, Shanghai 9th People s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China
Pediatr Res 63:492-6. 2008
..The field of tissue engineering will ultimately be advanced by increasing control of collagen in native tissue and by continual manipulation of biomaterials...
- Preparation and evaluation of thiolated chitosan scaffolds for tissue engineering
Zhe Li
National Tissue Engineering Center of China, Shanghai, China
J Biomed Mater Res A 92:973-8. 2010
..The preferential growth of fibroblasts on this scaffold was also demonstrated. Hence, results in this study would offer valuable information on the preparation of suitable TCS scaffolds for tissue engineering...
- Engineering of extensor tendon complex by an ex vivo approach
Bin Wang
Department of Plastic and Reconstructive Surgery, Shanghai 9th People s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, PR China
Biomaterials 29:2954-61. 2008
..These findings indicate that an extensor tendon complex like structure is possible to generate by an ex vivo approach and in vivo mechanical loading might be an optimal niche for engineering functional extensor tendon...
- Construction and clinical application of a human tissue-engineered epidermal membrane
Jun Yang
Department of Plastic and Reconstructive Surgery, Shanghai 9th People s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Plast Reconstr Surg 125:901-9. 2010
..3 mm are common problems that continue to challenge plastic surgeons in the clinic. As such, a human tissue-engineered epidermal membrane was constructed to promote wound healing and reduce scar hypertrophy...
- A sandwich model for engineering cartilage with acellular cartilage sheets and chondrocytes
Yi Yi Gong
Department of Plastic and Reconstructive Surgery, Shanghai 9th People s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China
Biomaterials 32:2265-73. 2011
..Furthermore, we achieved cartilage with a designed shape by pre-shaping the sheets prior to implantation. These results indicate that the sandwich model could be a useful model for engineering cartilage in vitro and in vivo...
- Tissue engineering of blood vessel
Wen Jie Zhang
Department of Plastic and Reconstructive Surgery, Shanghai 9th People s Hospital, Shanghai Jiao Tong University School of Medicine, National Tissue Engineering Center of China, Shanghai, China
J Cell Mol Med 11:945-57. 2007
..The remaining challenges are also discussed...
- A closer view of tissue engineering in China: the experience of tissue construction in immunocompetent animals
Wei Liu
Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, and Shanghai Tissue Engineering Center, Shanghai Second Medical University, Shanghai, People's Republic of China
Tissue Eng 9:S17-30. 2003
..The engineered tissues include bone, cartilage, tendon, skin, blood vessels, cornea, and peripheral nerves...
- Inhibiting scar formation in rat wounds by adenovirus-mediated overexpression of truncated TGF-beta receptor II
Wei Liu
Department of Plastic and Reconstructive Surgery, Shanghai Ninth People s Hospital, Shanghai Second Medical University, Shanghai Institute of Plastic and Reconstructive Surgery, Shanghai Tissue Engineering Center, P R China
Plast Reconstr Surg 115:860-70. 2005
..05). These results indicate that gene therapy by targeting wound TGF-beta can effectively inhibit wound scarring and may potentially be applied to clinical scar treatment...
- Expanded adipose-derived stem cells suppress mixed lymphocyte reaction by secretion of prostaglandin E2
Lei Cui
Department of Plastic and Reconstructive Surgery, 9th People s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People s Republic of China
Tissue Eng 13:1185-95. 2007
..These results indicated that in vitro expanded ADSCs retain low immunogenicity and immunosuppressive effect, and PGE2 might be the major soluble factor involved in the in vitro inhibition of allogeneic lymphocyte reaction...
- Tissue-engineered bone repair of goat-femur defects with osteogenically induced bone marrow stromal cells
Lian Zhu
Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Tissue Engineering Center, Shanghai Second Medical University, Shanghai, China
Tissue Eng 12:423-33. 2006
..Based on these results, we conclude that engineered bone from osteogenically induced BMSCs and coral can ideally heal critical-sized segmental bone defects in the weight-bearing area of goats...
- Tissue engineering of nearly transparent corneal stroma
Xiaojie Hu
Department of Plastic and Reconstructive Surgery, Ninth People's Hospital and Tissue Engineering Center, Shanghai Second Medical University, Shanghai, People's Republic of China
Tissue Eng 11:1710-7. 2005
..Marked losses of translucence during this period combined with restoration of ultrastructure indicate that the implants provide the functions needed for deturgescing initially swollen stroma...
- Creating thick linear scar by inserting a gelatin sponge into rat excisional wounds
Xiaoli Wu
Department of Plastic and Reconstructive Surgery, Shanghai 9th People s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Wound Repair Regen 15:595-606. 2007
..Moreover, this model may serve as a tool for studying gene-mediated tissue regeneration during wound repair using inserted gelatin sponge as a gene carrier...
- Application of scaffold materials in tissue reconstruction in immunocompetent mammals: our experience and future requirements
Wei Liu
Department of Plastic and Reconstructive Surgery, Shanghai 9th People s Hospital, Shanghai Jiao Tong University School of Medicine, National Tissue Engineering Center of China, 639 Zhi Zao Ju Road, Shanghai 200011, PR China
Biomaterials 28:5078-86. 2007
..Additionally, future requirements for scaffold application in tissue reconstruction and regeneration are proposed as well...
- The impact of PLGA scaffold orientation on in vitro cartilage regeneration
Yingying Zhang
Department of Plastic and Reconstructive Surgery, Shanghai 9th People s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, Shanghai, PR China
Biomaterials 33:2926-35. 2012
..These results indicated that the longitudinal microtubular orientation of scaffolds can efficiently improve the structure and function of in vitro engineered cartilage...
- Repairing large porcine full-thickness defects of articular cartilage using autologous chondrocyte-engineered cartilage
Yanchun Liu
Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai, People's Republic of China
Tissue Eng 8:709-21. 2002
..Thus, the results of this study may provide insight into the clinical repair of articular cartilage defects...
- HLA-G*0105N and HLA-G 14 bp dimorphisms in exon 8 in four distinct populations in mainland China
W Tian
Immunogenetics Research Group, Department of Immunology, College of Basic Medical Sciences, Central South University, Changsha, Hunan, China
Tissue Antigens 75:227-34. 2010
..The LD patterns uncovered will facilitate the understanding of HLA-G role in associations previously described between HLA-A subregion and diseases...
- Gene therapy of scarring: a lesson learned from fetal scarless wound healing
W Liu
Department of Plastic and Reconstructive Surgery, Shanghai Ninth People s Hospital, Shanghai Second Medical University, 639 Zhizaoju Road, Shanghai, 200011, P R China
Yonsei Med J 42:634-45. 2001
..Finally, the authors propose possible strategies of wound gene therapy, to reduce wound scarring and to promote tissue regeneration...
- Molecular cloning and characterization of a cDNA encoding soybean nodule IMP dehydrogenase
Y Cao
Department of Botany and Microbiology, University of Oklahoma, Norman, OK 73019 0245, USA
Biochim Biophys Acta 1520:242-6. 2001
..Southern blot analysis suggested a single copy of IMPDH gene in the soybean genome. Northern blot analysis showed that the expression of IMPDH gene is apparently nodule-specific...
- MICA-STR, HLA-B haplotypic diversity and linkage disequilibrium in the Hunan Han population of southern China
W Tian
Department of Immunology, Central South University Xiang Ya School of Medicine, Changsha, Hunan, China
Int J Immunogenet 33:241-5. 2006
....
- Residual undifferentiated cells during differentiation of induced pluripotent stem cells in vitro and in vivo
Wei Fu
Shanghai Key Laboratory of Tissue Engineering, Department of Plastic and Reconstructive Surgery, National Tissue Engineering Center of China, Shanghai Jiao Tong University School of Medicine, Shanghai 9th People s Hospital, Shanghai, China
Stem Cells Dev 21:521-9. 2012
..These findings prompt us to reconsider the strategies for solving the tumorigenic problem of iPS cells, not only focusing on improving the reprogramming process...
- Auricular reconstruction for microtia: personal 6-year experience based on 350 microtia ear reconstructions in China
Qun Zhang
Department of Plastic and Reconstructive Surgery, Shanghai 9th People s Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, People s Republic of China
Plast Reconstr Surg 123:849-58. 2009
..The authors report 350 patients with different types of microtia who underwent total ear reconstruction. The youngest was aged 5.5 years and the oldest was aged 50 years...
- The transfection of embryonic stem cells with Tet-on system and its responsiveness to doxycycline
Yan Wang
Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Second Medical University, Shanghai Key Laboratory of Tissue Engineering, China
Sci China C Life Sci 48:307-10. 2005
..This indicated that the ESCs transfected with Tet-on system could response to DOX very well, and the regulation of target gene expression is dose dependent...
- Efficient Schwann cell purification by differential cell detachment using multiplex collagenase treatment
Yu Qing Jin
Department of Plastic and Reconstructive Surgery, Shanghai 9th People s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Tissue Engineering Laboratory, Shanghai, China
J Neurosci Methods 170:140-8. 2008
..In addition, this efficient method can reach a high cell yield after two rounds of differential detachment procedures and does not require antimitotic treatment or special equipment as often needed by other reported methods...
- Engineering of various types of tissues in immunocompetent animals and its potential for clinical application
Y Cao
Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Second Medical University, Shanghai Tissue Engineering Center
Med J Malaysia 59:2. 2004
- Biomimetic scaffolds: implications for craniofacial regeneration
Jie Yuan
Department of Plastic and Reconstructive Surgery, Shanghai 9th People s Hospital, Shanghai Tissue Engineering Key Laboratory, Shanghai Jiao Tong University School of Medicine, Shanghai, China
J Craniofac Surg 23:294-7. 2012
..This article summarizes new developments of biomimetic scaffolds including the biomimesis of extracellular matrix, cell-matrix interaction, and tissue-regenerative signaling...
- Tissue-engineered bone repair of sheep cranial defects with autologous bone marrow stromal cells
Q Shang
Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Tissue Engineering Center, Shanghai Second Medical University, China
J Craniofac Surg 12:586-93; discussion 594-5. 2001
..Three-dimensional computerized tomography (CT) scan revealed an almost complete repair of the defect of experimental group at 18 weeks. This study may provide insight for future clinical repair of cranial defect...
- Porous poly (DL-lactic acid) modified chitosan-gelatin scaffolds for tissue engineering
Haifeng Liu
Research Institute of Polymeric Materials, Tianjin University, Tianjin 300072, China
J Biomater Appl 19:303-22. 2005
..1% concentration could increase the tensile strength fourfold in comparison with the pristine Cs-Gel scaffold, while maintaining the human fibroblast adhesion, migration, and proliferation just like the Cs-Gel scaffold...
- [Tissue engineered tendon with skin fibroblasts as seed cells, a preliminary study]
Bing Chen
The Research Center of Tissue Engineering, Department of Plastic Surgery, Ninth People's Hospital Affiliated to Shanghai Second Medical University, Shanghai 200011, China
Zhonghua Yi Xue Za Zhi 82:1105-7. 2002
..0 +/- 18.2 N and 18.9 +/- 1.9 MPa, reaching 57.4% and 51.9% of those of normal tendon respectively. CONCLUSION: Skin fibroblasts can be used as seed cells to regenerate tendon with normal structure and function...
- [Repair of porcine full-thickness skin defects with autologous tissue engineered skin]
Yilin Cao
Department of Plastic Reconstructive Surgery, Shanghai Ninth People's Hospital, The Key Laboratory of Tissue Engineering, Shanghai Second Medical University, Shanghai 200011, China
Zhonghua Wai Ke Za Zhi 40:24-6. 2002
..CONCLUSION: Tissue engineered skin can repair autologous full-thickness skin defects with primary-cultured keratinocytes and fibroblasts as seed cells and PGA as a cell carrier...
- [Repair of meniscal defects with autologous tissue-engineered fibrocartilage]
Yimin Cui
Tissue Engineering Research Center of Shanghai Second Medical University, Department of Plastic and Reconstructive Surgery, Shanghai No. 9 People's Hospital, Affiliated to Shanghai Second Medical University, Shanghai 200011, China
Zhonghua Yi Xue Za Zhi 82:191-3. 2002
..CONCLUSION: Autologous tissue-engineered fibrocartilage is a promising feasible method to regenerate or reconstruct menisci so as to hold back the degenerative changes of the knee...
- Tissue-engineered bone formation with cryopreserved human bone marrow mesenchymal stem cells
Guangpeng Liu
Shanghai Tissue Engineering Research and Development Center, Shanghai 200235, China
Cryobiology 56:209-15. 2008
..Based on these results, it can be concluded that cryopreservation allows human MSCs to be available for potential therapeutic use to tissue-engineer bone...
- Tissue-engineered bone formation using human bone marrow stromal cells and novel beta-tricalcium phosphate
Guangpeng Liu
National Tissue Engineering Research and Development Center, Shanghai 200235, People s Republic of China
Biomed Mater 2:78-86. 2007
..The above findings illustrate that the novel porous beta-TCP scaffolds developed in this work have prominent osteoconductive activity and the potential for applications in bone tissue engineering...
- Repair of goat tibial defects with bone marrow stromal cells and beta-tricalcium phosphate
Guangpeng Liu
Shanghai Tissue Engineering Research and Development Center, Shanghai, 200235, China
J Mater Sci Mater Med 19:2367-76. 2008
..Thus, it can be concluded that the mechanical properties of the BMSCs/beta-TCP composites could be much improved via tissue engineering approach and beta-TCP might be used to repair the weight-bearing segmental defects of goat tibias...
- Cryopreservation of tissue-engineered dermal replacement in Me2SO: Toxicity study and effects of concentration and cooling rates on cell viability
Xin Wang
Institute of Cryomedicine, Shanghai University of Science and Technology, Shanghai 200093, China
Cryobiology 55:60-5. 2007
..The micrograph obtained by electronic scanning microscope also confirmed this result...
- Bone reconstruction with bone marrow stromal cells
Wei Liu
Department of Plastic Surgery, Shanghai 9th People's Hospital, Shanghai, People's Republic of China
Methods Enzymol 420:362-80. 2006
..Our in vivo study in this aspect and the published results indicate that bone regeneration and repair by BMSCs and biodegradable scaffold is a realistic goal that can be achieved...
- A study on a chitosan-gelatin-hyaluronic acid scaffold as artificial skin in vitro and its tissue engineering applications
Haifeng Liu
Research Institute of Polymer Materials, Tianjin University, Tianjin 300072, Peoples Republic of China
J Biomater Sci Polym Ed 15:25-40. 2004
..The artificial skin obtained was flexible and had good mechanical properties. The data from this study suggested that chitosan-gelatin-hyaluronic acid scaffolds are suitable for preparing a bilayer skin substitute...
- Influence of the concentrations of hyaluronic acid on the properties and biocompatibility of Cs-Gel-HA membranes
Haifeng Liu
Research Institute of Polymer Materials, Tianjin University, Tianjin 300072, People's Republic of China
Biomaterials 25:3523-30. 2004
..01-0.1%), that could promote the cell adhesion, migration and proliferation, while the concentration of HA was above 0.1% it would either reduce or even inhibit these behaviors...
- Electroporation-mediated plasmid gene transfer in rat incisional wound
Zhen Gao
J Dermatol Sci 47:161-4. 2007
- Fabrication and surface modification of macroporous poly(L-lactic acid) and poly(L-lactic-co-glycolic acid) (70/30) cell scaffolds for human skin fibroblast cell culture
Jian Yang
Center for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China
J Biomed Mater Res 62:438-46. 2002
..The results suggest that anhydrous ammonia plasma treatment enhances the cell affinity of porous scaffolds. Mass transport issues also have been considered...
- Study of novel chitosan-gelatin artificial skin in vitro
Jinshu Mao
Research Institute of Polymeric Materials, Tianjin University, Tianjin 300072, Peoples Republic of China
J Biomed Mater Res A 64:301-8. 2003
..Moreover, there was no contraction observed in the in vitro cell culture tests. The data from this study suggest that chitosan-gelatin scaffolds are suitable for skin tissue engineering goals...
- [In vitro chondrogenic phenotype differentiation of bone marrow stem cells]
Wanyao Xia
Research Center of Tissue Engineering, Shanghai Second Medical University, Department of Plastic Surgery, Shanghai Ninth People's Hospital, Shanghai 200011, China
Zhonghua Zheng Xing Wai Ke Za Zhi 18:12-4. 2002
..CONCLUSIONS: Bone marrow stem cells obtained from adult swine can differentiate to be chondrogenic phenotype when cultured in vitro. MSCs can likely be served as optimal autogenous cell source for cartilage tissue engineering...
