Research Topics
Genomes and GenesSpecies | Bernard D LemireSummaryAffiliation: University of Alberta Country: Canada Publications
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Publications
The Saccharomyces cerevisiae mitochondrial succinate:ubiquinone oxidoreductaseBernard D Lemire
Canadian Institutes of Health Research Group in the Molecular Biology of Membrane Proteins, Department of Biochemistry, University of Alberta, Edmonton, AB T6G 2H7, Canada
Biochim Biophys Acta 1553:102-16. 2002..The yeast SDH provides the best opportunity for understanding the biogenesis of this family of iron-sulfur flavoproteins...- C. elegans longevity pathways converge to decrease mitochondrial membrane potentialBernard D Lemire
Department of Biochemistry, University of Alberta, Alberta, Canada
Mech Ageing Dev 130:461-5. 2009..Our results are consistent with the 'uncoupling to survive' hypothesis, which states that dissipation of the DeltaPsi(m) will extend lifespan... - Expression of Ndi1p, an alternative NADH:ubiquinone oxidoreductase, increases mitochondrial membrane potential in a C. elegans model of mitochondrial diseaseAdrienne DeCorby
Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
Biochim Biophys Acta 1767:1157-63. 2007..However, we have also shown that Ndi1p cannot substitute for the absence of complex I. Nevertheless, the yeast Ndi1p should be considered as a candidate for gene therapy in human diseases involving complex I... - The Saccharomyces cerevisiae succinate dehydrogenase does not require heme for ubiquinone reductionKayode S Oyedotun
Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
Biochim Biophys Acta 1767:1436-45. 2007..Our results strongly suggest that heme is not required for electron transport from succinate to quinone nor is it necessary for assembly of the S. cerevisiae SDH... - Mutations in the Saccharomyces cerevisiae succinate dehydrogenase result in distinct metabolic phenotypes revealed through (1)H NMR-based metabolic footprintingSamuel S W Szeto
Department of Biochemistry, School of Molecular and Systems Medicine, University of Alberta, Edmonton, Alberta, Canada
J Proteome Res 9:6729-39. 2010..Our study provides novel insight into the metabolic effects of SDH dysfunction and highlights the effectiveness of metabolic footprinting for examining complex disorders, such as mitochondrial diseases... - Mutations in the C. elegans succinate dehydrogenase iron-sulfur subunit promote superoxide generation and premature agingJingzhou Huang
Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada
J Mol Biol 387:559-69. 2009..Our work leads to a better understanding of the relationship between genotype and phenotype in respiratory chain mutations and of the mechanisms of aging and tumorigenesis... - Introduction of an additional pathway for lactate oxidation in the treatment of lactic acidosis and mitochondrial dysfunction in Caenorhabditis elegansLeslie I Grad
Department of Biochemistry, University of Alberta, Edmonton, AB, Canada T6G 2H7
Proc Natl Acad Sci U S A 102:18367-72. 2005.... - DiS-C3(3) monitoring of in vivo mitochondrial membrane potential in C. elegansDana Gaskova
Charles University, Faculty of Mathematics and Physics, Institute of Physics, Ke Karlovu 5, 12116 Prague 2, Czech Republic Department of Biochemistry, University of Alberta, Edmonton, Alta, Canada T6G 2H7
Biochem Biophys Res Commun 354:814-9. 2007..Our fluorescence assay will enable us to better dissect and understand the complex phenotypic consequences of mitochondrial dysfunction... - Mitochondrial ATP synthase controls larval development cell nonautonomously in Caenorhabditis elegansWilliam Y Tsang
The Canadian Institutes of Health Membrane Protein Research Group, Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada
Dev Dyn 226:719-26. 2003..We conclude that atp-2 functions cell nonautonomously in this developmental process. Our findings suggest that atp-2 is involved in the production or the regulation of a global, developmental signal required for the L3-to-L4 transition... - Caenorhabditis elegans development requires mitochondrial function in the nervous systemSarah Ndegwa
Department of Biochemistry, Membrane Protein Research Group of the Canadian Institutes of Health Research, University of Alberta, Edmonton, Alta, Canada T6G 2H7
Biochem Biophys Res Commun 319:1307-13. 2004..Our results indicate that MRC function in the nervous system is needed to send and receive signals that control larval development and exit from dauer... - The ubiquinone-binding site of the Saccharomyces cerevisiae succinate-ubiquinone oxidoreductase is a source of superoxideJing Guo
Canadian Institutes of Health, Membrane Protein Research Group, Department of Biochemistry, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
J Biol Chem 278:47629-35. 2003..Our data also challenge the dogma that superoxide production by SDH is a flavin-mediated event rather than a quinone-mediated one... - Ubiquinone-binding site mutations in the Saccharomyces cerevisiae succinate dehydrogenase generate superoxide and lead to the accumulation of succinateSamuel S W Szeto
Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada
J Biol Chem 282:27518-26. 2007..We suggest that SDH mutations can promote tumor formation by contributing to both reactive oxygen species production and to a proliferative response normally induced by hypoxia via the accumulation of succinate... - Isolation and functional analysis of mitochondria from the nematode Caenorhabditis elegansLeslie I Grad
Membrane Protein Research Group, Department of Biochemistry, University of Alberta, Edmonton, Canada
Methods Mol Biol 372:51-66. 2007..In this chapter, we describe the growth of C. elegans in liquid culture, the isolation of crude and purified mitochondria, and polarographic and histochemical approaches for measuring mitochondrial respiratory chain function... - Riboflavin enhances the assembly of mitochondrial cytochrome c oxidase in C. elegans NADH-ubiquinone oxidoreductase mutantsLeslie I Grad
Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
Biochim Biophys Acta 1757:115-22. 2006..We propose that one primary pathogenic mechanism of some complex I mutations is to destabilize complex IV. Enhancing complex I assembly with riboflavin results in the added benefit of partially reversing the complex IV deficit... - The role of Sdh4p Tyr-89 in ubiquinone reduction by the Saccharomyces cerevisiae succinate dehydrogenaseYuri Silkin
Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
Biochim Biophys Acta 1767:143-50. 2007..Our results support a role for Tyr-89 in the protonation of ubiquinone and argue that the generation of reactive oxygen species is not causative of tumor formation... - (1)H NMR-based metabolic profiling reveals inherent biological variation in yeast and nematode model systemsSamuel S W Szeto
Department of Biochemistry, School of Molecular and Systems Medicine, University of Alberta, Edmonton, AB, T6G 2H7, Canada
J Biomol NMR 49:245-54. 2011..We also highlight the efficacy of metabolic profiling for defining these subtle metabolic alterations... - Identification of the heme axial ligands in the cytochrome b562 of the Saccharomyces cerevisiae succinate dehydrogenaseKayode S Oyedotun
Canadian Institutes of Health Research Group in Membrane Protein Research, Department of Biochemistry, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
J Biol Chem 279:9432-9. 2004..Loss of cytochrome b(562) has no effect on enzyme assembly and quinone reduction; the role of the heme in enzyme structure and function is discussed... - The role of mitochondria in the life of the nematode, Caenorhabditis elegansWilliam Y Tsang
Canadian Institutes of Health Research Group in Membrane Protein Research, Department of Biochemistry, University of Alberta, 474 Medical Sciences Bldg, Edmonton, Alberta, Canada T6G 2H7
Biochim Biophys Acta 1638:91-105. 2003..We anticipate that further significant contributions to our understanding of mitochondrial function in animal biology are forthcoming with the C. elegans model system... - The quaternary structure of the Saccharomyces cerevisiae succinate dehydrogenase. Homology modeling, cofactor docking, and molecular dynamics simulation studiesKayode S Oyedotun
Canadian Institutes of Health Membrane Protein Research Group, Department of Biochemistry, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
J Biol Chem 279:9424-31. 2004..The simulation offers insight into why Sdh4p Cys-78 may be serving as the second axial ligand for the heme instead of a histidine residue. We discuss the possible roles of heme and of the two quinone-binding sites in electron transport... - Stable heteroplasmy but differential inheritance of a large mitochondrial DNA deletion in nematodesWilliam Y Tsang
Canadian Institutes of Health Research, Department of Biochemistry, University of Alberta, Edmonton
Biochem Cell Biol 80:645-54. 2002..These two forces prevent the segregation of the mtDNAs to homoplasmy... - Mitochondrial complex I mutations in Caenorhabditis elegans produce cytochrome c oxidase deficiency, oxidative stress and vitamin-responsive lactic acidosisLeslie I Grad
Canadian Institutes of Health Research Group in Membrane Protein Research, Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
Hum Mol Genet 13:303-14. 2004.... - Mitochondrial genome content is regulated during nematode developmentWilliam Y Tsang
Canadian Institutes of Health Research Group in the Molecular Biology of Membrane Proteins, University of Alberta, Edmonton, Alberta, T6G 2H7, Canada
Biochem Biophys Res Commun 291:8-16. 2002..Thus, mtDNA amplification is a necessary component of normal development and its regulation may involve an energy-sensing decision or checkpoint that can be invoked when mitochondrial energy generation is impaired... - The mitochondrial prohibitin complex is essential for embryonic viability and germline function in Caenorhabditis elegansMarta Artal-Sanz
Swammerdam Institute for Life Sciences, Section for Molecular Biology, University of Amsterdam, Kruislaan 318, Amsterdam 1098 SM, The Netherlands
J Biol Chem 278:32091-9. 2003....