Research Topics
| Aled EdwardsSummaryAffiliation: University of Toronto Country: Canada Publications
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Detail Information
Publications
MAID : an effect size based model for microarray data integration across laboratories and platformsIvan Borozan
Banting and Best Department of Medical Research, University of Toronto, 112 College St, Toronto, ON M5G1L6, Canada
BMC Bioinformatics 9:305. 2008..The increase in sensitivity and reliability of measurements of gene expression changes can be improved through a systematic integration of different microarray datasets that address the same or similar biological questions...- Protein production: feeding the crystallographers and NMR spectroscopistsA M Edwards
Ontario Cancer Institute, Toronto, Canada
Nat Struct Biol 7:970-2. 2000..Thousands of proteins are expected to be produced in the next few years. The purified proteins will be valuable reagents for the entire research community... - Origin DNA-binding proteinsA M Edwards
Banting and Best Department of Medical Research, University of Toronto, Ontario, Canada
Curr Opin Struct Biol 8:49-53. 1998..One of the origin-binding proteins, Epstein-Barr virus nuclear antigen 1, most likely has two modes of DNA binding; the sequential use of these modes may be important for the initiation of DNA replication... - Open access chemical and clinical probes to support drug discoveryAled M Edwards
Structural Genomics Consortium, University of Toronto, Toronto, Ontario, Canada
Nat Chem Biol 5:436-40. 2009..Chemical biologists could play a central role in this effort... - Large-scale structural biology of the human proteomeAled Edwards
Banting and Best Department of Medical Research, University of Toronto, Ontario, Canada
Annu Rev Biochem 78:541-68. 2009..Half of the unique human protein structures determined in the past three years derive from these initiatives... - The structural basis of gas-responsive transcription by the human nuclear hormone receptor REV-ERBbetaKeith I Pardee
Banting and Best Department of Medical Research, The Department of Molecular Genetics, University of Toronto, Toronto, Canada
PLoS Biol 7:e43. 2009..They also raise the possibility for gas-based therapies for the many disorders associated with REV-ERB biological functions... - The crystal structure of the complex of replication protein A subunits RPA32 and RPA14 reveals a mechanism for single-stranded DNA bindingA Bochkarev
Department of Medical Genetics and Microbiology, University of Toronto, 1 King s College Circle, Toronto, Ontario M5S 1A8
EMBO J 18:4498-504. 1999..The structure also suggests a mechanism for RPA trimer formation... - In situ proteolysis to generate crystals for structure determination: an updateAmy Wernimont
Midwest Center for Structural Genomics and Structural Genomics Consortium, University of Toronto, Toronto, Ontario, Canada
PLoS ONE 4:e5094. 2009..The overall success rate is similar to that observed in the smaller study, and appears to be higher than any other method reported to rescue stalled protein crystallography projects... - Structure of the single-stranded-DNA-binding domain of replication protein A bound to DNAA Bochkarev
Institute for Molecular Biology and Biotechnology, Cancer Research Group, McMaster University, Hamilton, Ontario, Canada
Nature 385:176-81. 1997..The ssDNA lies in a channel that extends from one subdomain to the other. The structure of each RPA70 subdomain is similar to those of the bacteriophage SSBs, indicating that the mechanism of ssDNA-binding is conserved... - Genome-scale protein expression and structural biology of Plasmodium falciparum and related Apicomplexan organismsMasoud Vedadi
Structural Genomics Consortium, U of Toronto, 100 College St Rm 522B, Toronto, Ont, Canada M5G 1L5
Mol Biochem Parasitol 151:100-10. 2007.... - The Drosophila nuclear receptor e75 contains heme and is gas responsiveJeff Reinking
Banting and Best Department of Medical Research, Charles H Best Institute, 112 College Street, Toronto, Ontario, M5G 1L6, Canada
Cell 122:195-207. 2005..Further, the E75-DHR3 interaction is also regulated by the binding of NO or CO to the heme center, suggesting that E75 may also function as a diatomic gas sensor. Possible mechanisms and roles for these interactions are discussed... - Solution structure of ribosomal protein S28E from Methanobacterium thermoautotrophicumBin Wu
Northeast Structural Genomics Consortium, Division of Molecular and Structural Biology, Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, Toronto, Ontario M5G 2M9, Canada
Protein Sci 12:2831-7. 2003..A broad positively charged surface extending over one side of the beta-barrel and into the flexible C terminus may present a putative binding site for RNA... - Structural proteomics of an archaeonD Christendat
Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto 610 University Ave, Toronto, Ontario, Canada M5G 2M9
Nat Struct Biol 7:903-9. 2000..This demonstrates that structural proteomics is feasible and can play a central role in functional genomics... - Insights into ligand binding and catalysis of a central step in NAD+ synthesis: structures of Methanobacterium thermoautotrophicum NMN adenylyltransferase complexesV Saridakis
Division of Molecular and Structural Biology, Ontario Cancer Institute, 610 University Avenue Toronto M5G 2M9, Ontario, Canada
J Biol Chem 276:7225-32. 2001..In addition, the role of the conserved (16)HXGH(19) active site motif in catalysis was probed by mutagenic, enzymatic and crystallographic techniques, including the characterization of an NMN(+)/SO4(2-) complex of mutant H19A NMNATase... - The precompetitive space: time to move the yardsticksThea Norman
Structural Genomics Consortium, University of Toronto, Toronto, ON M5G 1L7, Canada
Sci Transl Med 3:76cm10. 2011..The group will reconvene in April 2011 to create a business plan that specifies the generation of two positive POCM results per year... - High-throughput protein crystallizationRaymond Hui
Affinium Pharmaceuticals, 100 University Avenue, Ont, Toronto, Canada
J Struct Biol 142:154-61. 2003..Increasing the success rate will require a multidisciplinary approach that must invoke techniques from molecular biology, protein biochemistry, biophysics, artificial intelligence, and automation... - A chemical probe selectively inhibits G9a and GLP methyltransferase activity in cellsMasoud Vedadi
Structural Genomics Consortium, University of Toronto, Toronto, Ontario, Canada
Nat Chem Biol 7:566-74. 2011..In mouse embryonic stem cells, UNC0638 reactivated G9a-silenced genes and a retroviral reporter gene in a concentration-dependent manner without promoting differentiation... - The RPA32 subunit of human replication protein A contains a single-stranded DNA-binding domainE Bochkareva
Ontario Cancer Institute, Department of Medical Biophysics, University of Toronto, Toronto, Ontario M5G 2M9, Canada
J Biol Chem 273:3932-6. 1998..The ssDNA-binding affinity of this trimeric complex was 3 to 5-fold higher than hRPA14.32-(43-171) alone, suggesting a role for the C terminus of hRPA70 in ssDNA binding... - Binding of elongin A or a von Hippel-Lindau peptide stabilizes the structure of yeast elongin CM V Botuyan
Division of Molecular and Structural Biology, Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, 610 University Avenue, Toronto, ON, Canada M5G 2M9
Proc Natl Acad Sci U S A 96:9033-8. 1999..These results suggest a structural basis for the interaction of an F-box-containing protein with a SKP1 homologue and the modulation of this interaction by the tumor suppressor VHL... - Structure of a conserved domain common to the transcription factors TFIIS, elongin A, and CRSP70V Booth
Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, Toronto, Ontario M5G 2M9, Canada
J Biol Chem 275:31266-8. 2000.... - Crystal structure of dTDP-4-keto-6-deoxy-D-hexulose 3,5-epimerase from Methanobacterium thermoautotrophicum complexed with dTDPD Christendat
Division of Molecular and Structural Biology, Ontario Cancer Institute, Toronto, Ontario M5G 2M9, Canada
J Biol Chem 275:24608-12. 2000..The conservation of the active site residues suggests that the mechanism of action is also conserved and that the RmlC structure may be useful in guiding the design of antibacterial drugs... - Solution structure and dynamics of yeast elongin C in complex with a von Hippel-Lindau peptideM V Botuyan
Division of Molecular and Structural Biology, Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, Toronto, ON M5G 2M9, Canada
J Mol Biol 312:177-86. 2001..Moreover, the disappearance of conformational exchange phenomena on the microsecond to millisecond time scale within Elc1 upon VHL peptide binding suggests a role for slow internal motions in ligand recognition... - RNA polymerase II subunit Rpb9 regulates transcription elongation in vivoS A Hemming
Banting and Best Department of Medical Research, University of Toronto, Charles H Best Institute, Toronto, Ontario M5G 1L6, Canada
J Biol Chem 275:35506-11. 2000..Genome-wide transcription profiling of Deltarpb9 cells revealed only a few changes, predominantly in genes related to metabolism... - Elongin from Saccharomyces cerevisiaeC M Koth
Department of Medical Genetics and Microbiology, University of Toronto, Toronto, Ontario M5S 1A8
J Biol Chem 275:11174-80. 2000..These studies revealed that yeast elongin A is unfolded but undergoes a dramatic modification of its structure in the presence of elongin C, and that elongin C forms a stable dimer in the absence of elongin A... - Dissociable Rpb4-Rpb7 subassembly of rna polymerase II binds to single-strand nucleic acid and mediates a post-recruitment step in transcription initiationS M Orlicky
Banting and Best Department of Medical Research and Department of Medical Genetics and Microbiology, C H Best Institute, University of Toronto, Ontario M5G 1L6, Canada
J Biol Chem 276:10097-102. 2001.... - Gene expression profiling of acute liver stress during living donor liver transplantationI Borozan
Banting and Best Department of Medical Research, University of Toronto, Toronto, Ontario, Canada
Am J Transplant 6:806-24. 2006..This study has identified a set of genes whose expression is altered in acute, but not chronic, liver stress, likely to play a central role in the pathogenesis of acute liver injury of liver transplantation...