Research Topics
Genomes and Genes | Raymond S W TsangSummaryAffiliation: Public Health Agency of Canada Country: Canada Publications
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Publications
Serological diagnosis of syphilis: enzyme-linked immunosorbent assay to measure antibodies to individual recombinant Treponema pallidum antigensIrene E Martin
Pathogenic Neisseria and Syphilis Diagnostic Section, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada
J Immunoassay Immunochem 29:143-51. 2008..Further evaluations with sera from different stages of syphilis can help to define the applications of this ELISA test for each of the four antigens studied...- Genetic and antigenic analysis of invasive serogroup Y Neisseria meningitidis isolates collected from 1999 to 2003 in CanadaRaymond S W Tsang
Laboratory for Vaccine Preventable Bacterial Diseases, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada R3E 3R2
J Clin Microbiol 45:1753-8. 2007..5,2. In contrast to previous reports that patients with serogroup Y disease are usually older, 26% of the Canadian serogroup Y cases were found in the 10-to-19-year-old age group and another 11% were in the 20-to-39-year-old age group... - Immunochemical studies and genetic background of two Neisseria meningitidis isolates expressing unusual capsule polysaccharide antigens with specificities of both serogroup Y and W135Raymond S W Tsang
Laboratory for Vaccine Preventable Bacterial Diseases, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, MB R3E3R2, Canada
Can J Microbiol 54:229-34. 2008..How these strains may affect results of routine surveillance, PCR diagnosis, and immuno-protection by vaccination are discussed... - Characterization of invasive Haemophilus influenzae disease in Manitoba, Canada, 2000-2006: invasive disease due to non-type b strainsRaymond S W Tsang
Vaccine Preventable Bacterial Diseases, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada
Clin Infect Dis 44:1611-4. 2007..influenzae disease was found to be approaching the rates of H. influenzae type b disease that were documented in the prevaccine period. Fifty-six percent of invasive disease now occurs in individuals aged >10 years... - Invasive Haemophilus influenzae in Manitoba, Canada, in the postvaccination eraRaymond S W Tsang
Vaccine Preventable Bacterial Disease Section, National Microbiology Laboratory, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3R2
J Clin Microbiol 44:1530-5. 2006..These results suggest a change in the epidemiology of H. influenzae disease, with the majority of invasive H. influenzae isolates being associated with serotype a and NST strains... - Serological diagnosis of syphilis: comparison of the Trep-Chek IgG enzyme immunoassay with other screening and confirmatory testsRaymond S W Tsang
Laboratory for Pathogenic Neisseria, Syphilis Serology, and Vaccine Preventable Bacterial Diseases, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada
FEMS Immunol Med Microbiol 51:118-24. 2007..3% and 95.6%, respectively. Without further evaluation, we do not recommend use of the Trep-Chek IgG EIA as a stand-alone test for either screening or confirmatory syphilis serology... - Characterization of nontypeable Haemophilus influenzae collected from respiratory infections and invasive disease cases in Manitoba, CanadaMichelle Shuel
National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, Canada
FEMS Immunol Med Microbiol 58:277-84. 2010..Besides a change in the capsule status of invasive Hi strains, the burden of invasive Hi disease, which used to be mainly a childhood disease, has now shifted to involve both adults and infants... - Genetic and antigenic analysis of Bordetella pertussis isolates recovered from clinical cases in Ontario, Canada, before and after the introduction of the acellular pertussis vaccineRaymond S W Tsang
Vaccine Preventable Bacterial Disease Division, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, Canada
Can J Microbiol 51:887-92. 2005..Further surveillance with clinical data and isolates collected periodically will be required to ensure that any genetic divergence that could affect vaccine efficacy will not be occurring... - Population genetics and antibiotic susceptibility of invasive Haemophilus influenzae in Manitoba, Canada, from 2000 to 2006Michelle L Sill
Vaccine Preventable Bacterial Diseases, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, Canada
FEMS Immunol Med Microbiol 51:270-6. 2007..The results of this study suggest a shift in the epidemiology of invasive H. influenzae infections in the post-Hib vaccine era, and surveillance should include all serotypeable and NST isolates... - Molecular typing of Treponema pallidum strains in western Canada: predominance of 14d subtypesIrene E Martin
Division of Pathogenic Neisseria, Syphilis Diagnostics, and Vaccine Preventable Bacterial Diseases, National Microbiology Laboratory, Public Health Agency of Canada, Ottawa, Ontario, Canada
Sex Transm Dis 37:544-8. 2010.... - Macrolide resistance and molecular types of Treponema pallidum causing primary syphilis in Shanghai, ChinaIrene E Martin
Division of Pathogenic Neisseria, Syphilis Diagnostics, Vaccine Preventable Bacterial Diseases, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada
Clin Infect Dis 49:515-21. 2009..This study focuses on implementing polymerase chain reaction (PCR) diagnostic tests for syphilis and for detection of molecular subtypes and macrolide resistance among strains causing primary syphilis in the city of Shanghai, China... - Invasive meningococcal disease in Quebec, Canada, due to an emerging clone of ST-269 serogroup B meningococci with serotype antigen 17 and serosubtype antigen P1.19 (B:17:P1.19)Dennis K S Law
National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada
J Clin Microbiol 44:2743-9. 2006..The emergence of this clone of serogroup B meningococci occurred after a mass vaccination against serogroup C N. meningitidis, suggesting possible capsule replacement... - Nucleotide sequence diversity of the bexA gene in serotypeable Haemophilus influenzae strains recovered from invasive disease patients in CanadaJianwei Zhou
Vaccine Preventable Bacteria Diseases, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada R3E 32R2
J Clin Microbiol 45:1996-9. 2007..This sequence diversity may lead to false-negative PCR results for H. influenzae infections if bexA is the chosen gene target... - Molecular characterization of four Haemophilus influenzae serotype a strains isolated from patients in Quebec, CanadaMichelle L Sill
Vaccine Preventable Bacterial Diseases, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, MB R3E 3R2, Canada
Can J Microbiol 53:1191-4. 2007..Whether this genetic diversity is related to the severity and epidemiology of Hia disease requires further studies... - Canadian beta-lactamase-negative Haemophilus influenzae isolates showing decreased susceptibility toward ampicillin have significant penicillin binding protein 3 mutationsMichelle L Shuel
Vaccine Preventable Bacterial Diseases, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada
Diagn Microbiol Infect Dis 63:379-83. 2009..The difficulty in detection of BLNAR strains was discussed... - Antigenic and genetic characterization of serogroup C meningococci isolated from invasive meningococcal disease cases in Canada from 1999 to 2003Dennis K S Law
Laboratory of Pathogenic Neisseria and Vaccine Preventable Bacterial Diseases, National Microbiology Laboratory, Canada
Can J Microbiol 51:523-30. 2005..Besides the clonal group of ET-15/ET-37, small numbers of serogroup C isolates were found to belong to the clonal complexes of ST-8 (Cluster A4), ST-41/44 (Lineage 3), ST-35, and ST-269... - Antibiotic susceptibility of invasive Haemophilus influenzae strains in CanadaMichelle L Sill
Vaccine Preventable Bacterial Diseases, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada
Antimicrob Agents Chemother 52:1551-2. 2008..5% were beta-lactamase negative ampicillin intermediate, and 2.5% were intermediate to only the 2-microg ampicillin disk. An increase in beta-lactamase-negative ampicillin-intermediate strains has been found... - Serological specificities of murine hybridoma monoclonal antibodies against Neisseria meningitidis serogroups B, C, Y, and W135 and evaluation of their usefulness as serogrouping reagents by indirect whole-cell enzyme-linked immunosorbent assayRaymond S W Tsang
Laboratory for Pathogenic Neisseria and Vaccine Preventable Bacterial Diseases, National Microbiology Laboratory, Health Canada, 1015 Arlington St, Winnipeg, Manitoba, Canada R3E 3R2
Clin Diagn Lab Immunol 12:152-6. 2005..The advantage of using MAbs for serogrouping of meningococci is discussed... - Molecular characterization of syphilis in patients in Canada: azithromycin resistance and detection of Treponema pallidum DNA in whole-blood samples versus ulcerative swabsIrene E Martin
Division of Pathogenic Neisseria, Syphilis Diagnostics, and Vaccine Preventable Bacterial Diseases, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba R3E 3R2, Canada
J Clin Microbiol 47:1668-73. 2009..However, further research is required to determine what specimen(s) would be most appropriate for molecular investigation of syphilis in secondary and latent syphilis... - Outbreak of serogroup C meningococcal disease caused by a variant of Neisseria meningitidis serotype 2a ET-15 in a community of men who have sex with menRaymond S W Tsang
National Microbiology Laboratory, Population and Public Health Branch, Health Canada Winnipeg, Manitoba, Canada
J Clin Microbiol 41:4411-4. 2003..A microbiological characterization of the outbreak strain is presented in this report... - Polymorphisms of the fimbria fim3 gene of Bordetella pertussis strains isolated in CanadaRaymond S W Tsang
National Microbiology Laboratory, Health Canada, Winnipeg, Manitoba, Canada R3E 3R2
J Clin Microbiol 42:5364-7. 2004..At least one of the novel mutations identified was found to involve a surface epitope that has been mapped by serum antibodies from infected or vaccinated subjects... - DNA Sequence analysis of the PorB protein of nonserotypeable serogroup C ET-15 meningococci suggests a potential mutational hot spot on their serotype antigensDennis K S Law
CNS Infection and Vaccine Preventable Bacterial Diseases Division, National Microbiology Laboratory, 1015 Arlington St, Winnipeg, Manitoba R3E 3R2, Canada
J Clin Microbiol 42:2718-23. 2004..This finding of a potential novel mutational hot spot on the PorB proteins of meningococci may have implications for pathogenesis and vaccine development... - Phenotypic and genetic characterization of a unique variant of serogroup C ET-15 meningococci (with the antigenic formula C:2a:P1.7,1) causing invasive meningococcal disease in Quebec, CanadaRaymond S W Tsang
CNS Infection Division, National Microbiology Laboratory, Health Canada, Winnipeg, Manitoba
J Clin Microbiol 42:1460-5. 2004..7,1. Strains of C:2a:P1.7,1 meningococci were not isolated in Canada in large numbers prior to 2001, and the characteristics of these meningococcal strains linked to an outbreak in Quebec, Canada, are described in the present study... - Use of monoclonal antibodies to serotype Bordetella pertussis isolates: comparison of results obtained by indirect whole-cell enzyme-linked immunosorbent assay and bacterial microagglutination methodsRaymond S W Tsang
Vaccine Preventable Bacterial Diseases Division, National Microbiology Laboratory, Population and Public Health Branch, Health Canada, Winnipeg, Manitoba, Canada
J Clin Microbiol 43:2449-51. 2005..Both methods gave concordant results in 60 of the 61 isolates... - Nonencapsulated or nontypeable Haemophilus influenzae are more likely than their encapsulated or serotypeable counterparts to have mutations in their fucose operonMichelle L Shuel
Vaccine Preventable Bacterial Diseases, National Microbiology Laboratory, 1015 Arlington Street, Public Health Agency of Canada, Winnipeg, MB R3E 3R2, Canada
Can J Microbiol 57:982-6. 2011..The significance of these findings is discussed... - Molecular analysis of monoclonal antibodies to group variant capsular polysaccharide of Neisseria meningitidis: recurrent heavy chains and alternative light chain partnersJody D Berry
National Center for Foreign Animal Disease, Canadian Food Inspection Agency, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3M4
Mol Immunol 42:335-44. 2005..Knowledge of the specific immunoglobulin genes used to target common bacterial virulence factors may lead to insights on pathogen-host co-evolution, and the potential use of this information in pre-symptomatic diagnosis is discussed... - A novel interpretation of structural dot plots of genomes derived from the analysis of two strains of Neisseria meningitidisWilfred R Cuff
Public Health Agency of Canada, Canadian Science Centre for Human and Animal Health, Winnipeg, Canada
Genomics Proteomics Bioinformatics 8:159-69. 2010.... - International circumpolar surveillance interlaboratory quality control program for serotyping Haemophilus influenzae and serogrouping Neisseria meningitidis, 2005 to 2009Raymond S W Tsang
National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada
J Clin Microbiol 50:651-6. 2012..These data demonstrate a high degree of concordance for serogroup and serotype determinations of N. meningitidis and H. influenzae isolates, respectively, among the five laboratories participating in this quality control program... - Serotyping and population genetics of invasive Haemophilus influenzaeRaymond S W Tsang
J Clin Microbiol 46:1159. 2008 - Genetic diversity of three lgt loci for biosynthesis of lipooligosaccharide (LOS) in Neisseria speciesPeixuan Zhu
Division of Bacterial, Parasitic and Allergenic Products and Division of Viral Products, Center for Biologics Evaluation and Research, FDA, 8800 Rockville Pike, Bethesda, MD 20892, USA
Microbiology 148:1833-44. 2002..The data demonstrated that pathogenic and commensal Neisseria share a common lgt gene pool and horizontal gene transfer appears to contribute to the genetic diversity of the lgt loci in Neisseria... - Increase in serogroup C meningococcal disease in Canada is associated with antigenic changes in the protein antigens of the ET-15 clone of Neisseria meningitidisRaymond S W Tsang
J Infect Dis 194:1791-2; author reply 1792-3. 2006 - Identification of opcA gene in Neisseria polysaccharea: interspecies diversity of Opc protein familyPeixuan Zhu
Division of Bacterial, Parasitic and Allergenic Products, Center for Biologics Evaluation and Research, FDA, Bethesda, MD 20892, USA
Gene 307:31-40. 2003..The data presented here provide additional information for understanding the distribution, variation and expression of opcA in Neisseria... - Nonencapsulated Neisseria meningitidis strain produces amylopectin from sucrose: altering the concept for differentiation between N. meningitidis and N. polysacchareaPeixuan Zhu
Division of Bacterial, Parasitic and Allergenic Products, Center for Biologics Evaluation and Research, U S Food and Drug Administration, Bethesda, Maryland 20892, USA
J Clin Microbiol 41:273-8. 2003..polysaccharea by horizontal genetic exchange. Therefore, we conclude that genetic analysis is required to complement the traditional phenotypic classification for the nonencapsulated Neisseria strains... - Analysis of phenotypic variants of the serogroup C ET-15 clone of Neisseria meningitidis by pulsed-field gel electrophoresisMarissa L Cameron
J Clin Microbiol 45:2351-2. 2007