Gene therapy by electroporation for the treatment of chronic renal failure in companion animalsPatricia A Brown
VGX Animal Health, 2700 Research Forest Drive, Suite 180, The Woodlands, Texas 77381, USA
BMC Biotechnol 9:4. 2009
..4 mg or 0.1 mg species-specific plasmid injection, respectively, intramuscularly followed by electroporation, and analyzed up to 75 days post-treatment; controls underwent electroporation without plasmid administration...
- Optimization of electroporation parameters for the intramuscular delivery of plasmids in pigs
Amir S Khan
ADViSYS, Inc, The Woodlands, Texas 77381, USA
DNA Cell Biol 22:807-14. 2003
..These results demonstrate that electroporation parameters such as amperage, lag time, and the number of pulses are able to regulate the levels of reporter gene expression in pigs...
- Double-blinded, Placebo-controlled plasmid GHRH trial for cancer-associated anemia in dogs
Angela M Bodles-Brakhop
VGX Pharmaceuticals, The Woodlands, Texas 77381, USA
Mol Ther 16:862-70. 2008
..Overall, the possibility of a GHRH plasmid-based therapy for anemia in cancer-afflicted subjects is important enough to deserve further investigation...
- Plasmid-based growth hormone-releasing hormone supplementation and its applications
Amir S Khan
ADViSYS Inc, 2700 Research Forest Drive, Suite 180, The Woodlands, TX 77381, USA
Curr Opin Mol Ther 7:306-16. 2005
..The positive results obtained with plasmid-based GHRH in companion and farm animals may be translated to a number of human applications...
- Parameters for DNA vaccination using adaptive constant-current electroporation in mouse and pig models
Ruxandra Draghia-Akli
VGX Pharmaceuticals, 2700 Research Forest Drive, Suite 180, The Woodlands, TX 77381, United States
Vaccine 26:5230-7. 2008
..Furthermore, various conditions of EP that limit the amount of muscle damage were measured. The results of these studies will help to advance the success of DNA vaccines in animals into success in human clinical trials...
- Maternal GHRH plasmid administration changes pituitary cell lineage and improves progeny growth of pigs
Amir S Khan
ADViSYS, Inc, The Woodlands, TX 77381, USA
Am J Physiol Endocrinol Metab 285:E224-31. 2003
..The similarity of the response across species suggests that the effect is likely exerted as a fundamental component of gestational and developmental physiology...
- Immune-enhancing effects of growth hormone-releasing hormone delivered by plasmid injection and electroporation
Patricia A Brown
ADViSYS, Inc, 2700 Research Forest Drive, Suite 180, The Woodlands, TX 77381, USA
Mol Ther 10:644-51. 2004
..The plasmid injection followed by electroporation could prove an efficient method for the systemic production of therapeutic proteins and may provide a useful means for basic research in relevant animal models...
- Delivery of DNA into skeletal muscle in large animals
Patricia A Brown
VGX Animal Health, The Woodlands, TX, USA
Methods Mol Biol 423:215-24. 2008
..These results could be extrapolated for a different muscle group in pigs, the biceps femoris, and may be an evaluation starting point for large muscle in veterinary species or humans (see Note 1 )...
- Plasmid growth hormone releasing hormone therapy in healthy and laminitis-afflicted horses-evaluation and pilot study
Patricia A Brown
VGX Animal Health, The Woodlands, Texas 77381, USA
J Gene Med 10:564-74. 2008
..In vivo electroporation dramatically improves the potency of plasmid-mediated therapies, including in large animal models. Laminitis and arthritis are common and debilitating diseases in the horse, as well as humans...
- Plasmid-based expression technology using growth hormone releasing hormone: a novel method for physiologically stimulating long-term growth hormone secretion
Ruxandra Draghia-Akli
ADViSYS, Inc, The Woodlands, Texas 77381, USA
Comb Chem High Throughput Screen 9:181-5. 2006
..Using the species-specific sequences of GHRH or other molecule along with the appropriate choice of plasmid backbone and expression cassette components can result in long and steady expression of the transgene product...
- Effects of plasmid growth hormone-releasing hormone treatment during heat stress
Patricia A Brown
VGX Animal Health, The Woodlands, Texas 77381, USA
DNA Cell Biol 27:629-35. 2008
..3% vs. 30.8%). This study shows that the use of plasmid-mediated therapy delivered by EP can maintain health status during periods of heat stress, important for both animals and potentially humans in hot, challenging climates...
- Electrical enhancement of formulated plasmid delivery in animals
Ruxandra Draghia-Akli
ADViSYS, Inc, 2700 Research Forest Drive, Suite 180, The Woodlands, Texas 77381, USA
Technol Cancer Res Treat 1:365-72. 2002
..Muscle lesions were reduced by the protective PLG. Thus, PLG may constitute a useful adjuvant for increased expression and reduced muscle trauma to plasmid DNA delivered by electroporation...
