Survival after T cell-depleted haploidentical stem cell transplantation is improved using the mother as donorMartin Stern
Department of Hematology, University Hospital Basel, Basel, Switzerland
Blood 112:2990-5. 2008
..Although obtained in a retrospective analysis, these data suggest that the mother of the patient should be preferred as donor for haploidentical HSCT...
- Phenotypical/functional characterization of in vitro-expanded mesenchymal stromal cells from patients with Crohn's disease
Maria Ester Bernardo
Oncoematologia Pediatrica, Fondazione IRCCS Policlinico San Matteo, Universita di Pavia, Pavia, Italy
Cytotherapy 11:825-36. 2009
..The aim of the study was in vitro characterization of MSC from active CD patients for future clinical application...
- Co-infusion of ex vivo-expanded, parental MSCs prevents life-threatening acute GVHD, but does not reduce the risk of graft failure in pediatric patients undergoing allogeneic umbilical cord blood transplantation
M E Bernardo
Pediatric Hematology Oncology, Fondazione IRCCS Policlinico San Matteo, University of Pavia, Pavia, Italy
Bone Marrow Transplant 46:200-7. 2011
..Although these data do not support the use of MSCs in UCBT to support hematopoietic engraftment, they suggest that MSCs, possibly because of their immunosuppressive effect, may abrogate life-threatening acute GVHD and reduce early TRM...
- Human bone marrow derived mesenchymal stem cells do not undergo transformation after long-term in vitro culture and do not exhibit telomere maintenance mechanisms
Maria Ester Bernardo
Oncoematologia Pediatrica, Fondazione Istituto di Ricovero e Cura a Carattere Scientifico Policlinico San Matteo, Universita di Pavia, Pavia, Italy
Cancer Res 67:9142-9. 2007
..ALT was not evidenced in the MSCs tested. BM-derived MSCs can be safely expanded in vitro and are not susceptible to malignant transformation, thus rendering these cells suitable for cell therapy approaches...
- Optimization of in vitro expansion of human multipotent mesenchymal stromal cells for cell-therapy approaches: further insights in the search for a fetal calf serum substitute
M E Bernardo
Oncoematologia Pediatrica, IRCCS Policlinico San Matteo, Universita di Pavia, Pavia, Italy
J Cell Physiol 211:121-30. 2007
....
- Treosulfan-based conditioning regimen for allogeneic haematopoietic stem cell transplantation in patients with thalassaemia major
Maria Ester Bernardo
Oncoematologia Pediatrica, Fondazione IRCCS Policlinico San Matteo, Universita di Pavia, Italy
Br J Haematol 143:548-51. 2008
..This regimen might find elective application in patients at high risk of developing life-threatening complications...
- Human mesenchymal stem cells derived from bone marrow display a better chondrogenic differentiation compared with other sources
M E Bernardo
Pediatric Hematology Oncology, Fondazione IRCCS Policlinico San Matteo, University of Pavia, Pavia, Italy
Connect Tissue Res 48:132-40. 2007
..As MSCs represent an attractive tool for cartilage tissue repair strategies, our data suggest that bone marrow should be considered the preferred MSC source for these therapeutic approaches...
- Generation of mesenchymal stromal cells in the presence of platelet lysate: a phenotypic and functional comparison of umbilical cord blood- and bone marrow-derived progenitors
Maria Antonietta Avanzini
Oncoematologia Pediatrica, Fondazione IRCCS Policlinico San Matteo, Universita di Pavia, Pavia, Italy
Haematologica 94:1649-60. 2009
..However, relatively little is known about the mechanisms responsible for their immunomodulatory effects, which could be influenced by both the cell source and culture conditions...
- Preemptive therapy of EBV-related lymphoproliferative disease after pediatric haploidentical stem cell transplantation
P Comoli
Laboratory of Transplant Immunology and Pediatric Hematology Oncology, Fondazione IRCCS Policlinico S Matteo, University of Pavia, Pavia, Italy
Am J Transplant 7:1648-55. 2007
..We conclude that preemptive therapy with rituximab is safe, but only partly effective in haplo-HSCT recipients. Patients who progress to PTLD under rituximab treatment can be rescued permanently by infusion of EBV-specific CTLs...
- The outcome of children with Fanconi anemia given hematopoietic stem cell transplantation and the influence of fludarabine in the conditioning regimen: a report from the Italian pediatric group
Franco Locatelli
Oncoematologia Pediatrica, Fondazione IRCCS Policlinico San Matteo e Università di Pavia, Pavia, Italy
Haematologica 92:1381-8. 2007
..While in the past HSCT from an HLA-identical sibling was proven to cure many patients, a higher incidence of treatment failure has been reported in recipients of an unrelated donor (UD) or HLA-partially matched related allograft...
- The role of reduced intensity preparative regimens in patients with thalassemia given hematopoietic transplantation
Alice Bertaina
Department of Paediatric Haematology Oncology, University of Pavia, Ospedale Bambino Gesu, Rome, Italy
Ann N Y Acad Sci 1202:141-8. 2010
..This novel approach is a promising alternative for reducing the risk of life-threatening complications and increasing the number of TM patients successfully cured with an allograft...
- Low percentages of circulating CD8(+)/CD45RA(+) human T lymphocytes expressing beta7 integrin correlate with the occurrence of intestinal acute graft-versus-host disease after allogeneic hematopoietic stem cell transplantation
M Antonietta Avanzini
Pediatric Hematology-Oncology and Laboratory of Transplant Immunology, IRCCS Policlinico San Matteo-Pavia, University of Pavia, Pavia, Italy
Exp Hematol 34:1429-34. 2006
....
- Mesenchymal stromal cells
Maria Ester Bernardo
Oncoematologia Pediatrica, Fondazione IRCCS Policlinico San Matteo, Universita di Pavia, Pavia, Italy
Ann N Y Acad Sci 1176:101-17. 2009
....
- Familial platelet disorder with propensity to acute myelogenous leukemia: genetic heterogeneity and progression to leukemia via acquisition of clonal chromosome anomalies
Antonella Minelli
Biologia Generale e Genetica Medica, , Pavia, Italy
Genes Chromosomes Cancer 40:165-71. 2004
....
- Clofarabine, cyclophosphamide and etoposide as single-course re-induction therapy for children with refractory/multiple relapsed acute lymphoblastic leukaemia
Franco Locatelli
Oncoematologia Pediatrica, Fondazione IRCCS Policlinico San Matteo, Universita di Pavia, Italy
Br J Haematol 147:371-8. 2009
..01). These data suggest that the clofarabine/cyclophosphamide/etoposide regimen is well tolerated and can induce clinical response in a relevant proportion of children with refractory/multiple relapsed ALL...
- Use of a DNAemia cut-off for monitoring human cytomegalovirus infection reduces the number of preemptively treated children and young adults receiving hematopoietic stem-cell transplantation compared with qualitative pp65 antigenemia
Daniele Lilleri
Servizio di Virologia, Universita di Pavia, Fondazione Istituto di Ricovero e Cura a Carattere Scientifico IRCCS Policlinico San Matteo, Pavia, Italy
Blood 110:2757-60. 2007
..259), the number of patients treated was significantly lower in the DNAemia arm (18% vs 31%, P = .026). No patient developed HCMV disease. The use of a DNAemia cut-off avoids unnecessary antiviral treatment...
- T-cell lines specific for peptides of adenovirus hexon protein and devoid of alloreactivity against recipient cells can be obtained from HLA-haploidentical donors
Patrizia Comoli
Pediatric Hematology Oncology, Fondazione IRCCS Policlinico S Matteo, University of Pavia, Pavia, Italy
J Immunother 31:529-36. 2008
..These T cells may be safely employed for adoptive treatment of HAdV complications...
- Single-cell cloning of human, donor-derived antileukemia T-cell lines for in vitro separation of graft-versus-leukemia effect from graft-versus-host reaction
Daniela Montagna
Department of Pediatrics, Laboratory of Immunology, University of Pavia, Italy
Cancer Res 66:7310-6. 2006
....
- Donor multipotent mesenchymal stromal cells may engraft in pediatric patients given either cord blood or bone marrow transplantation
Sara Pozzi
Centro Cellule Staminali e Terapia Cellulare, Ospedale San Martino, Genova, Italy
Exp Hematol 34:934-42. 2006
..CONCLUSIONS: BM of pediatric patients might be a more favorable milieu than that of adults for sustained engraftment of transplanted MSCs. MSCs able to engraft in the host can be transferred with cryopreserved CB units...
- Cotransplantation of ex vivo expanded mesenchymal stem cells accelerates lymphocyte recovery and may reduce the risk of graft failure in haploidentical hematopoietic stem-cell transplantation
Lynne M Ball
Department of Pediatric Stem Cell Transplantation, Leiden University Medical Centre, Leiden, The Netherlands
Blood 110:2764-7. 2007
..These data suggest that MSCs, possibly thanks to their potent immunosuppressive effect on alloreactive host T lymphocytes escaping the preparative regimen, reduce the risk of graft failure in haploidentical HSC transplant recipients...
