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Genomes and Genes | A TiborSummaryAffiliation: University of Namur Country: Belgium Publications
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Publications
Effect of P39 gene deletion in live Brucella vaccine strains on residual virulence and protective activity in miceA Tibor
Laboratoire de microbiologie et d immunologie, Facultes Universitaires Notre Dame de la Paix, B 5000 Namur, Belgium
Infect Immun 66:5561-4. 1998..CD-1 mice vaccinated with the parent or mutant strains were protected against a virulent challenge. Mutant vaccine strains devoid of P39 could provide a means for differentiating vaccinated from infected animals...- Outer membrane proteins Omp10, Omp16, and Omp19 of Brucella spp. are lipoproteinsA Tibor
Laboratoire de microbiologie et d immunologie, Facultes Universitaires Notre Dame de la Paix, B 5000 Namur, Belgium
Infect Immun 67:4960-2. 1999..The three proteins were radioimmunoprecipitated from [(3)H]palmitic acid-labeled Brucella abortus lysates with monoclonal antibodies. These results demonstrate that Omp10, Omp16, and Omp19 are lipoproteins... - Identification of the perosamine synthetase gene of Brucella melitensis 16M and involvement of lipopolysaccharide O side chain in Brucella survival in mice and in macrophagesF Godfroid
Unité de Recherche en Biologie Moléculaire URBM, Laboratoire d Immunologie et de Microbiologie, Facultes Universitaires Notre Dame de la Paix, Namur, Belgium
Infect Immun 66:5485-93. 1998..The survival of B. melitensis mutant strain B3B2 in the mouse model and in bovine macrophages was examined. The results suggested that S-LPS or, more precisely, its O side chain is essential for survival in mice but not in macrophages... - FtcR is a new master regulator of the flagellar system of Brucella melitensis 16M with homologs in RhizobiaceaeS Leonard
Unité de Recherche en Biologie Moléculaire, Laboratoire d Immunologie Microbiologie, Facultes Universitaires Notre Dame de la Paix, Namur, Belgium
J Bacteriol 189:131-41. 2007..Finally, FtcR binds directly to the upstream region of the fliF gene. Therefore, our data support the role of FtcR as a flagellar master regulator in B. melitensis and perhaps in other related alpha-proteobacteria... - Molecular characterization, occurrence, and immunogenicity in infected sheep and cattle of two minor outer membrane proteins of Brucella abortusA Tibor
Laboratoire de microbiologie et d immunologie, Facultes Universitaires Notre Dame de la Paix, Namur, Ghent, Belgium
Infect Immun 64:100-7. 1996..abortus-infected cattle were almost completely unreactive in this assay... - Characterization, occurrence, and molecular cloning of a 39-kilodalton Brucella abortus cytoplasmic protein immunodominant in cattleP A Denoel
Laboratoire de microbiologie et d immunologie, Faculté Universitaires Notre Dame de la Paix, Namur, Belgium
Infect Immun 65:495-502. 1997..The nucleotide sequence of the P39 gene was determined, and the deduced amino acid sequence is in accordance with the sequence of an internal peptide isolated from P39... - Characterization of smooth lipopolysaccharides and O polysaccharides of Brucella species by competition binding assays with monoclonal antibodiesV Weynants
Unité d Immunologie Microbiologie, Facultes Universitaires Notre Dame de la Paix, Namur, Belgium
Infect Immun 65:1939-43. 1997..Competitive binding assays between these monoclonal antibodies suggested that these different epitopes are probably overlapping structures... - Survival of a bacterioferritin deletion mutant of Brucella melitensis 16M in human monocyte-derived macrophagesP A Denoel
U R Biologie Moléculaire, Laboratoire de microbiologie et d immunologie, Facultes Universitaires Notre Dame de la Paix, Namur, Belgium
Infect Immun 65:4337-40. 1997..melitensis PAD 2-78, were similar to those of its parental strain in human monocyte-derived macrophages (MDM). These results suggest that BFR is not essential for the intracellular survival of B. melitensis in human MDM... - Structure and function prediction of the Brucella abortus P39 protein by comparative modeling with marginal sequence similaritiesK de Fays
Unité de Recherche en Biologie Moléculaire, Facultes Universitaires Notre Dame de la Paix, Namur, Belgium
Protein Eng 12:217-23. 1999..Knowing the genetic context of the P39 reading frame, we are now able to suggest a function for the P39 protein: it would act as a periplasmic substrate-binding protein... - Molecular cloning, nucleotide sequence, and occurrence of a 16.5-kilodalton outer membrane protein of Brucella abortus with similarity to pal lipoproteinsA Tibor
Laboratoire de microbiologie et d immunologie, Facultes Universitaires Notre Dame de la Paix, Belgium
Infect Immun 62:3633-9. 1994..In immunoblotting with a monoclonal antibody specific for Omp16, we demonstrated that Omp16 was expressed in the 34 Brucella strains tested, representing all six species and known biovars... - Fun stories about Brucella: the "furtive nasty bug"J J Letesson
Laboratoire d Immunologie et de Microbiologie, Unité de Recherche en Biologie Moléculaire, Facultes Universitaires Notre Dame de la Paix, Rue de Bruxelles 61, B 5000 Namur, Belgium
Vet Microbiol 90:317-28. 2002..In this paper, we summarize some of the research in progress in our laboratory that we think could contribute to a better understanding of the Brucella molecular virulence mechanisms and their regulation... - Cloning and sequencing of the bacterioferritin gene of Brucella melitensis 16M strainP A Denoel
Laboratoire de microbiologie et d immunologie, Facultes Universitaires Notre Dame de la Paix, Namur, Belgium
FEBS Lett 361:238-42. 1995..The sequence of the Brucella bacterioferritin is 69% similar to that of E. coli, and many of the ferroxidase centre and haem-ligation residues are conserved... - Protection of BALB/c mice against Brucella abortus 544 challenge by vaccination with bacterioferritin or P39 recombinant proteins with CpG oligodeoxynucleotides as adjuvantA Al-Mariri
, Laboratoire d'Immunologie et de Microbiologie, , B-5000 Namur, Belgium
Infect Immun 69:4816-22. 2001..Intriguingly, no protection was detected after BFR vaccination. All other groups did not demonstrate any protection... - Induction of immune response in BALB/c mice with a DNA vaccine encoding bacterioferritin or P39 of Brucella sppA Al-Mariri
, Laboratoire d'Immunologie et de Microbiologie, , B-5000 Namur, Belgium
Infect Immun 69:6264-70. 2001..The pCIP39 delivered by intramuscular injection (but not the pCIBFR or control vectors) induced a moderate protection in BALB/c mice challenged with B. abortus 544 compared to that observed in positive control mice vaccinated with S19... - Attenuated signature-tagged mutagenesis mutants of Brucella melitensis identified during the acute phase of infection in miceP Lestrate
, Laboratoire d'Immunologie et de Microbiologie, University of Namur, 5000 Namur, Belgium
Infect Immun 71:7053-60. 2003..The results presented here highlight the fact that the ability of Brucella to induce pathogenesis shares similarities with the molecular mechanisms used by both Rhizobium and Agrobacterium to colonize their hosts... - The sheathed flagellum of Brucella melitensis is involved in persistence in a murine model of infectionD Fretin
Unité de Recherche en Biologie Moléculaire, University of Namur, Belgium
Cell Microbiol 7:687-98. 2005..In contrast, all these mutants were unable to establish a chronic infection in mice infected via the intraperitoneal route, raising the question of the biological role(s) of this flagellar appendage...