Research Topics
Genomes and Genes | Jean Jacques LetessonSummaryAffiliation: University of Namur Country: Belgium Publications
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Publications
Fun stories about Brucella: the "furtive nasty bug"J J Letesson
Laboratoire d Immunologie et de Microbiologie, Unité de Recherche en Biologie Moléculaire, Facultes Universitaires Notre Dame de la Paix, Rue de Bruxelles 61, B 5000 Namur, Belgium
Vet Microbiol 90:317-28. 2002..In this paper, we summarize some of the research in progress in our laboratory that we think could contribute to a better understanding of the Brucella molecular virulence mechanisms and their regulation...- A quorum-sensing regulator controls expression of both the type IV secretion system and the flagellar apparatus of Brucella melitensisRose May Delrue
Research Unit in Molecular Biology URBM, University of Namur, 61 rue de Bruxelles, Namur, Belgium
Cell Microbiol 7:1151-61. 2005..In agreement with this hypothesis, we observed that, as virB operon, flagellar genes are controlled by the pheromone. All together these data support a model in which VjbR acts as a major regulator of virulence factors in Brucella... - DNA polymorphism analysis of Brucella lipopolysaccharide genes reveals marked differences in O-polysaccharide biosynthetic genes between smooth and rough Brucella species and novel species-specific markersMichel S Zygmunt
INRA, UR1282, Infectiologie Animale et Sante Publique, IASP, Nouzilly, France
BMC Microbiol 9:92. 2009..In smooth brucellae, lipopolysaccharide is made of lipid A-core oligosaccharide and N-formylperosamine O-polysaccharide. B. ovis and B. canis (rough species) lack the O-polysaccharide... - Humoral immune responses of Brucella-infected cattle, sheep, and goats to eight purified recombinant Brucella proteins in an indirect enzyme-linked immunosorbent assayJ J Letesson
URBM, Immunology Laboratory, FUNDP, Namur, Belgium
Clin Diagn Lab Immunol 4:556-64. 1997..None of the recombinant antigens detected experimentally infected nonpregnant cows and sheep or naturally infected cattle. This study is a first step towards the development of a multiprotein diagnostic reagent for brucellosis... - Morphological analysis of the sheathed flagellum of Brucella melitensisJonathan Ferooz
Unité de Recherche en Biologie Moléculaire URBM, Facultes Universitaires Notre Dame de la Paix FUNDP, 61 rue de Bruxelles, B 5000 Namur, Belgium
BMC Res Notes 3:333. 2010..It was recently shown that B. melitensis is flagellated. However, the flagellar structure remains poorly described... - Effect of omp10 or omp19 deletion on Brucella abortus outer membrane properties and virulence in miceAnne Tibor
Unité de Recherche en Biologie Moléculaire, Facultes Universitaires Notre Dame de la Paix, B 5000 Namur, Belgium
Infect Immun 70:5540-6. 2002..The omp19 mutant exhibited an increase in sensitivity to the polycation polymyxin B and to sodium deoxycholate. These results indicate that inactivation of the omp19 gene alters the outer membrane properties of B. abortus... - Identification of a Brucella spp. secreted effector specifically interacting with human small GTPase Rab2Marie de Barsy
URBM, University of Namur FUNDP, Belgium
Cell Microbiol 13:1044-58. 2011..abortus?ricA mutant in HeLa cells, compared with the wild-type control. Altogether, the data reported here suggest RicA as the first reported effector with a proposed function for B. abortus... - The Ton system, an ABC transporter, and a universally conserved GTPase are involved in iron utilization by Brucella melitensis 16MIsabelle Danese
Unité de Recherche en Biologie Moléculaire, University of Namur, Belgium
Infect Immun 72:5783-90. 2004..None of the three mutants was attenuated in cellular models or in the mouse model of infection, which is consistent with the previous suggestion that DHBA utilization is not required in these models... - Plasticity of a transcriptional regulation network among alpha-proteobacteria is supported by the identification of CtrA targets in Brucella abortusAnne Flore Bellefontaine
Unité de Recherche en Biologie Moléculaire URBM, Facultes Universitaires Notre Dame de la Paix, 61, rue de Bruxelles, B 5000 Namur, Belgium
Mol Microbiol 43:945-60. 2002..crescentus, similar cellular processes are regulated by CtrA through the control of distinct target genes. The plasticity of the regulation network involving CtrA in these two bacteria may be related to their distinct lifestyles... - RpoE1, an extracytoplasmic function sigma factor, is a repressor of the flagellar system in Brucella melitensisJonathan Ferooz
Unité de Recherche en Biologie Moléculaire URBM, Facultés Universitaires Notre Dame de la Paix Namur FUNDP, 61 rue de Bruxelles, B 5000 Namur, Belgium
Microbiology 157:1263-8. 2011..Together, these data show that RpoE1 represses the flagellar synthesis and filament length in B. melitensis... - Identification of a quorum-sensing signal molecule in the facultative intracellular pathogen Brucella melitensisBernard Taminiau
Unité de Recherche en Biologie Moléculaire URBM, Laboratoire d Immunologie et Microbiologie, Facultes Universitaires Notre Dame de la Paix, 5000 Namur, Belgium
Infect Immun 70:3004-11. 2002..This mimics events seen during the stationary phase of growth and suggests that quorum sensing may play a role in the control of virulence in Brucella... - Overproduced Brucella abortus PdhS-mCherry forms soluble aggregates in Escherichia coli, partially associating with mobile foci of IbpA-YFPCharles Van der Henst
Molecular Biology Research Unit URBM, University of Namur FUNDP, 61 rue de Bruxelles, 5000 Namur, Belgium
BMC Microbiol 10:248. 2010..These structures are associated with chaperones like IbpA. However, there are reported cases of "non-classical" inclusion bodies in which proteins are soluble, folded and active... - PdhS, an old-pole-localized histidine kinase, recruits the fumarase FumC in Brucella abortusJohann Mignolet
Molecular Biology Research Unit URBM, University of Namur FUNDP, 61 rue de Bruxelles, B 5000 Namur, Belgium
J Bacteriol 192:3235-9. 2010..FumC homologs are not polarly localized in Sinorhizobium meliloti and Caulobacter crescentus, suggesting that polar recruitment of FumC by PdhS is evolutionarily recent... - The alkylation response protein AidB is localized at the new poles and constriction sites in Brucella abortusDelphine Dotreppe
URBM, University of Namur FUNDP, 61 rue de Bruxelles, Namur, 5000, Belgium
BMC Microbiol 11:257. 2011..Brucella abortus is the etiological agent of a worldwide zoonosis called brucellosis. This alpha-proteobacterium is dividing asymmetrically, and PdhS, an essential histidine kinase, was reported to be an old pole marker... - Brucella melitensis 16M produces a mannan and other extracellular matrix components typical of a biofilmMarie Godefroid
Unité de Recherche en Biologie Moléculaire, Facultes Universitaires Notre Dame de la Paix, Namur, Belgium
FEMS Immunol Med Microbiol 59:364-77. 2010..Taken together, these data reinforce the evidence that B. melitensis could form biofilms in its lifecycle... - Functional, molecular and structural characterisation of five anti-Brucella LPS mAbThierry Cl Laurent
Unité de Recherche en Biologie Moléculaire, Facultes Universitaires, Notre Dame de la Paix, Rue de Bruxelles 61, B 5000 Namur, Belgium
Mol Immunol 40:1237-47. 2004..The analysis of their 3D structure gives new hypothesis of the epitopes recognised... - The stringent response mediator Rsh is required for Brucella melitensis and Brucella suis virulence, and for expression of the type IV secretion system virBMarie Dozot
Research Unit in Molecular Biology, University of Namur, 61 rue de Bruxelles, B 5000 Namur, Belgium
Cell Microbiol 8:1791-802. 2006.... - Global analysis of quorum sensing targets in the intracellular pathogen Brucella melitensis 16 MSophie Uzureau
Unité de Recherche en Biologie Moléculaire, Laboratoire d Immunologie Microbiologie, FUNDP University of Namur, Namur, Belgium
J Proteome Res 9:3200-17. 2010.... - Gateway-based destination vectors for functional analyses of bacterial ORFeomes: application to the Min system in Brucella abortusRégis Hallez
Unité de Recherche en Biologie Moléculaire, University of Namur, 61 rue de Bruxelles, B 5000 Namur, Belgium
Appl Environ Microbiol 73:1375-9. 2007..In an attempt to validate several of these vectors, we observed polar and oscillating localization of MinD in Brucella abortus... - Role of FlbT in flagellin production in Brucella melitensisJonathan Ferooz
Unité de Recherche en Biologie Moléculaire URBM, Facultés Universitaires Notre Dame de la Paix Namur FUNDP, 61 rue de Bruxelles, B 5000 Namur, Belgium
Microbiology 157:1253-62. 2011..This mechanism could be conserved in the Rhizobiales since complementation of an flbT or an ftcR mutant with flbT from Sinorhizobium meliloti restores FliC synthesis... - Identification of the essential Brucella melitensis porin Omp2b as a suppressor of Bax-induced cell death in yeast in a genome-wide screeningGéraldine Laloux
Research Unit in Molecular Biology, Department of Biology, University of Namur FUNDP, Namur, Belgium
PLoS ONE 5:e13274. 2010..Brucellae, which are the causative agent of a worldwide zoonosis, prevent apoptosis of infected cells, probably to support survival of their replication niche... - The Omp85 protein of Neisseria meningitidis is required for lipid export to the outer membraneStéphanie Genevrois
Research Unit in Molecular Biology URBM, University of Namur FUNDP, rue de Bruxelles, 61, 5000 Namur, Belgium
EMBO J 22:1780-9. 2003..Omp85 depletion did not affect localization of integral outer membrane proteins PorA and Opa. These results provide compelling evidence for a role for Omp85 in lipid transport to the outer membrane... - MyD88-dependent activation of B220-CD11b+LY-6C+ dendritic cells during Brucella melitensis infectionRichard Copin
Unité de Recherche en Biologie Moléculaire, Laboratoire d Immunologie et de Microbiologie, Faculté Universitaire Notre Dame de la Paix, Namur, Belgium
J Immunol 178:5182-91. 2007..Taken together, our results provide new insight into how immune responses fight Brucella infection... - Mutations of the quorum sensing-dependent regulator VjbR lead to drastic surface modifications in Brucella melitensisSophie Uzureau
Facultes Universitaires Notre Dame de la Paix, Unité de Recherche en Biologie Moléculaire, Laboratoire d Immunologie Microbiologie, Rue de Bruxelles 61, 5000 Namur, Belgium
J Bacteriol 189:6035-47. 2007..melitensis could form biofilms. Together, these results indicate that QS through VjbR is a major regulatory system of important cell surface structures of Brucella and as such plays a key role in host-pathogen interactions... - The asymmetric distribution of the essential histidine kinase PdhS indicates a differentiation event in Brucella abortusRégis Hallez
Unité de Recherche en Biologie Moléculaire, University of Namur FUNDP, Namur, Belgium
EMBO J 26:1444-55. 2007..Altogether, these data suggest that prokaryotic differentiation may be much more widespread than expected... - Design and implementation of a database for Brucella melitensis genome annotationBenoît De Hertogh
U R B M, F U N D P, rue de Bruxelles, 61, B 5000 Namur, Belgium
Vet Microbiol 127:369-78. 2008..The database is publicly available through a Web server at the following url: http://www.fundp.ac.be/urbm/bioinfo/aPAGe... - FtcR is a new master regulator of the flagellar system of Brucella melitensis 16M with homologs in RhizobiaceaeS Leonard
Unité de Recherche en Biologie Moléculaire, Laboratoire d Immunologie Microbiologie, Facultes Universitaires Notre Dame de la Paix, Namur, Belgium
J Bacteriol 189:131-41. 2007..Finally, FtcR binds directly to the upstream region of the fliF gene. Therefore, our data support the role of FtcR as a flagellar master regulator in B. melitensis and perhaps in other related alpha-proteobacteria... - Intracellular rescuing of a B. melitensis 16M virB mutant by co-infection with a wild type strainC Nijskens
Unité de Recherche en Biologie Moléculaire URBM, University of Namur, Namur, Belgium
Microb Pathog 45:134-41. 2008..Finally, vacuoles containing the rescued DeltavirB mutant were shown to exclude the LAMP-1 marker in a way similar to the WT containing vacuoles... - The sheathed flagellum of Brucella melitensis is involved in persistence in a murine model of infectionD Fretin
Unité de Recherche en Biologie Moléculaire, University of Namur, Belgium
Cell Microbiol 7:687-98. 2005..In contrast, all these mutants were unable to establish a chronic infection in mice infected via the intraperitoneal route, raising the question of the biological role(s) of this flagellar appendage... - Attenuated signature-tagged mutagenesis mutants of Brucella melitensis identified during the acute phase of infection in miceP Lestrate
, Laboratoire d'Immunologie et de Microbiologie, University of Namur, 5000 Namur, Belgium
Infect Immun 71:7053-60. 2003..The results presented here highlight the fact that the ability of Brucella to induce pathogenesis shares similarities with the molecular mechanisms used by both Rhizobium and Agrobacterium to colonize their hosts...