Research Topics
Genomes and GenesSpecies | F R OpperdoesSummaryCountry: Belgium Publications
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Publications
Enzymes of carbohydrate metabolism as potential drug targetsF R Opperdoes
Christian de Duve Institute of Cellular Pathology, ICP TROP 74 39, Avenue Hippocrate 74, B 1200, Brussels, Belgium
Int J Parasitol 31:482-90. 2001....
Comparison and evolutionary analysis of the glycosomal glyceraldehyde-3-phosphate dehydrogenase from different KinetoplastidaV Hannaert
Research Unit for Tropical Diseases, Christian de Duve Institute of Cellular Pathology ICP, and Laboratory of Biochemistry, Catholic University of Louvain, Brussels, Belgium
J Mol Evol 47:728-38. 1998..The division of the Kinetoplastida into two suborders, Bodonina and Trypanosomatina, was well supported. Within the letter group, the Trypanosoma species appeared to be monophyletic, whereas the other trypanosomatids form a second clade...
The glycosomal ATP-dependent phosphofructokinase of Trypanosoma brucei must have evolved from an ancestral pyrophosphate-dependent enzymeP A Michels
International Institute of Cellular and Molecular Pathology and Laboratory of Biochemistry, Catholic University of Louvain, Brussels, Belgium
Eur J Biochem 250:698-704. 1997..brucei PFK shows many structural differences, even at the active site, when compared with other ATP-dependent PFKs. These differences offer great potential for the structure-based design of trypanocidal drugs...
Trypanosoma brucei contains a 2,3-bisphosphoglycerate independent phosphoglycerate mutaseN Chevalier
Research Unit for Tropical Diseases, Christian de Duve Institute of Cellular Pathology and Laboratory of Biochemistry, Universite Catholique de Louvain, Brussels, Belgium
Eur J Biochem 267:1464-72. 2000..The presence of distinct nonhomologous PGAMs in the parasite and its human host offers great potential for the design of selective inhibitors which could form leads for new trypanocidal drugs...
Molecular characterization of the first two enzymes of the pentose-phosphate pathway of Trypanosoma brucei. Glucose-6-phosphate dehydrogenase and 6-phosphogluconolactonaseF Duffieux
Research Unit for Tropical Diseases, Christian de Duve Institute of Cellular Pathology and the Laboratory of Biochemistry, Universite Catholique de Louvain, Avenue Hippocrate 74, 1200 Brussels, Belgium
J Biol Chem 275:27559-65. 2000..The corresponding enzymes have been overexpressed in Escherichia coli and purified, and their biochemical characteristics have been determined...
Molecular cloning and analysis of two tandemly linked genes for pyruvate kinase of Trypanosoma bruceiS Allert
International Institute of Cellular and Molecular Pathology, Research Unit for Tropical Diseases, Brussels, Belgium
Eur J Biochem 200:19-27. 1991..The polypeptide is shorter than that of most other pyruvate kinases and the calculated net charge is only +3...
Glucosephosphate isomerase from Trypanosoma brucei. Cloning and characterization of the gene and analysis of the enzymeM Marchand
International Institute of Cellular and Molecular Pathology, Research Unit for Tropical Diseases, Brussels, Belgium
Eur J Biochem 184:455-64. 1989..Agaricic acid, a potent inhibitor of various glycosomal enzymes of T. brucei, has also a strong, irreversible effect on glucose-6-phosphate isomerase, while leaving the yeast and mammalian enzymes relatively unaffected...
Demonstration of glycosomes (microbodies) in the Bodonid flagellate Trypanoplasma borelli (Protozoa, Kinetoplastida)F R Opperdoes
International Institute of Cellular and Molecular Pathology, , Brussels, Belgium
Mol Biochem Parasitol 30:155-63. 1988..Apparently the presence of glycosomes is a characteristic of all Kinetoplastida...
Characterization of the genes for fructose-bisphosphate aldolase in Trypanosoma bruceiM Marchand
International Institute of Cellular and Molecular Pathology, Research Unit for Tropical Diseases, Brussels, Belgium
Mol Biochem Parasitol 29:65-75. 1988..We have previously proposed that the positively charged residues may be involved in the import of the proteins into the organelles...
Possible localisation of dolichol-dependent mannosyltransferase of Trypanosoma brucei to the rough endoplasmic reticulumM Prado-Figueroa
Research Unit for Tropical Diseases, International Institute of Cellular and Molecular Pathology, Brussels, Belgium
Mol Biochem Parasitol 63:255-64. 1994..It is concluded that the formation of dolichol phosphoryl mannose from GDP-mannose and dolichyl phosphate in T. brucei occurs mainly in the rough endoplasmic reticulum...
Localisation of a 3-hydroxy-3-methylglutaryl-coenzyme A reductase in the mitochondrial matrix of Trypanosoma brucei procyclicsN Heise
Christian de Duve Institute of Cellular Pathology ICP, and Laboratory of Biochemistry, Catholic University of Louvain, Brussels, Belgium
Z Naturforsch C 55:473-7. 2000..brucei procyclics is the explanation for a high 3-hydroxy-3-methylglutaryl-coenzyme A reductase activity in these protozoan organelles...
The electrochemical proton gradient in the bloodstream form of Trypanosoma brucei is dependent on the temperatureB H ter Kuile
Research Unit for Tropical Diseases, International Institute of Cellular and Molecular Pathology, Brussels, Belgium
Mol Biochem Parasitol 55:21-7. 1992..brucei when it resides in the bloodstream of the mammalian host at 37 degrees C; and (iii) that below 26 degrees C an electrochemical proton gradient is maintained over the plasma membrane...
Alkyl dihydroxyacetone phosphate synthase in glycosomes of Trypanosoma bruceiA W Zomer
Centre for Biomembranes and Lipid Enzymology, Utrecht, The Netherlands
Biochim Biophys Acta 1257:167-73. 1995..It is concluded that T. brucei glycosomes contain the enzymes responsible for glycero-ether bond formation analogous to mammalian peroxisomes...
Glyceraldehyde-phosphate dehydrogenase from Trypanosoma brucei. Comparison of the glycosomal and cytosolic isoenzymesO Misset
Eur J Biochem 162:501-7. 1987..This observation explains in part the great difference in subunit size between the two T. brucei isoenzymes and suggests that at least one of these insertions is responsible for import of the glycosomal isoenzyme into the organelle...
Glycolysis as a target for the design of new anti-trypanosome drugsC L Verlinde
Department of Biological Structure, Biomolecular Structure Center, University of Washington, Seattle, USA
Drug Resist Updat 4:50-65. 2001..For some trypanosomatid enzymes, potent and selective inhibitors have already been developed that affect only the growth of cultured trypanosomatids, and not mammalian cells...
The Trypanosoma cruzi proteomeJ A Atwood
Complex Carbohydrate Research Center, University of Georgia, Athens, GA 30602, USA
Science 309:473-6. 2005..The four parasite stages appear to use distinct energy sources, including histidine for stages present in the insect vectors and fatty acids by intracellular amastigotes...
The extraordinary mitochondrion and unusual citric acid cycle in Trypanosoma bruceiJ J Van Hellemond
Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht University, 3584 CM Utrecht, The Netherlands
Biochem Soc Trans 33:967-71. 2005..Therefore the citric acid cycle in trypanosomes does not function as a cycle...
