Research Topics
| B J LossonSummaryCountry: Belgium Publications
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Publications
Evaluation of a SRS2 sandwich commercial enzyme-linked immunosorbent assay for the detection of anti-Neospora caninum antibodies in bovine and canine seraFarida Ghalmi
Parasitic Diseases Department, Faculty of Veterinary Medicine, Laboratory of Parasitology, University of Liege, Liege, Belgium
J Vet Diagn Invest 21:108-11. 2009..The kappa value was 1 for bovine sera and 0.77 for canine sera. The seroprevalence was 3.9% in bovine sera and 21-23% in canine sera. The SRS2 sandwich ELISA was considered a valuable tool in both species...- Prevalence of Echinococcus multilocularis in the red fox (Vulpes vulpes) in southern BelgiumB Losson
Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liege, B43 Sart Tilman, B 4000 Liege, Belgium
Vet Parasitol 117:23-8. 2003..However, the latter was significantly higher in juveniles (<8 months of age). The geographical distribution of E. multilocularis in Belgium is much wider than originally thought... - The pathology of Psoroptes ovis infestation in cattle with a special emphasis on breed differenceB J Losson
Department of Parasitology and Pathology of Parasitic Diseases, Faculty of Veterinary Medicine, University of Liege, Belgium
Vet Parasitol 83:219-29. 1999..It is concluded that immunological as well as non-immunological factors may be responsible for the breed related susceptibility or resistance to P. ovis... - First isolation of Parafilaria bovicola from clinically affected cattle in BelgiumB Losson
Laboratory of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liege, 4000 Liege, Belgium
Vet Rec 164:623-6. 2009..The clinical herd prevalence in the area was 14.1 per cent (95 per cent confidence interval 11.3 to 17.2 per cent), suggesting that bovine parafilariosis is established in the area... - Immune response and antigen recognition in non-pregnant ewes experimentally infected with Neospora caninum tachyzoitesChantal Rettigner
Department of Infectious and Parasitic Diseases, Laboratory of Parasitology and Pathology of Parasitic Diseases, Faculty of Veterinary Medicine, University of Liege, Boulevard de Colonster B43a, 20, 4000 Liege, Belgium
Vet Parasitol 122:261-71. 2004..These antigens could be related to the acute stage of the infection... - Histopathological pattern and humoral immune response to a crude exo-antigen and purified keratinase of Microsporum canis in symptomatic and asymptomatic infected catsB R Mignon
University of Liege, Faculty of Veterinary Medicine, Department of Parasitology and Parasitic Diseases, Belgium
Med Mycol 37:1-9. 1999..Anti-keratinase specific IgG was only detected in one of the infected cats... - First epidemiological study on exposure to Neospora caninum in different canine populations in the Algiers District (Algeria)F Ghalmi
University of Liege, Faculty of Veterinary Medicine, Department of Infectious Diseases, Liege, Belgium
Parasitol Int 58:444-50. 2009..This study indicates that the level of exposure of the canine population of Algiers area to N. caninum is very high. This would indicate a potentially high risk for N. caninum induced abortion in cattle in this region and in Algeria... - The in vitro and in vivo production of a 31.5-kD keratinolytic subtilase from Microsporum canis and the clinical status in naturally infected catsB R Mignon
Department of Parasitology, Faculty of Veterinary Medicine, University of Liege, Belgium
Dermatology 196:438-41. 1998..The important variability of the clinical signs in cats is poorly understood. Recently, a 31.5-kD keratinolytic subtilase was found to be a putative virulence factor... - Clinical, pathological and diagnostic aspects of congenital neosporosis in a series of naturally infected calvesF De Meerschman
Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, , Boulevard de Colonster 20, , Belgium
Vet Rec 157:115-8. 2005 - The efficacy of moxidectin 0.5% pour-on against Hypoderma bovis in naturally infested cattle: parasitological and serological dataJ F Lonneux
Department of Parasitology and Parasitic Diseases, University of Liege, Belgium
Vet Parasitol 52:313-20. 1994..05). In conclusion, moxidectin 0.5% pour-on formulation at a dose of 0.5 mg kg-1 bodyweight can effectively treat cattle naturally infested with Hypoderma bovis first instar larvae... - Echinococcus multilocularis in Belgium: prevalence in red foxes (Vulpes vulpes) and in different species of potential intermediate hostsR Hanosset
Parasitology and Parasitic Diseases Faculty of Veterinary Medicine, University of Liege, Boulevard de Colonster 20, B 43, B4000 Liege, Belgium
Vet Parasitol 151:212-7. 2008.... - Fasciola hepatica: an assessment on the vectorial capacity of Radix labiata and R. balthica commonly found in BelgiumY Caron
Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liege, B 4000 Liege, Belgium
Vet Parasitol 149:95-103. 2007..R. labiata shed fewer metacercariae than G. truncatula but these were as infective to rats as those shed by G. truncatula. This study demonstrates that R. labiata may act as an incidental intermediate host for F. hepatica in Belgium... - Use of excretory/secretory antigens in a competition test to follow the kinetics of infection by Fasciola hepatica in cattleT Leclipteux
Department of Parasitology and Parasitic Disease, Faculty of Veterinary Medicine, Liege, Belgium
Vet Parasitol 77:103-14. 1998..The animals were slaughtered at the end of the experiment, the livers recovered and post-mortem fluke burdens determined. However, only serum liver enzyme levels gave any indication of the intensity of infection in the different animals... - In vitro entomopathogenic activity of Beauveria bassiana against Psoroptes spp. (Acari: Psoroptidae)M Lekimme
Laboratory of Parasitology and Pathology of Parasitic Diseases, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liege, Boulevard de Colonster 20 B43, B 4000 Liege, Belgium
Vet Parasitol 139:196-202. 2006..It is concluded that B. bassiana has a high entomopathogenic activity against Psoroptes spp. The in vivo use of this biocontrol agent against Psoroptes spp. in rabbit, sheep and cattle deserves further attention... - In vitro tests for evaluation of the hatchability of the eggs of Psoroptes mites following exposure to acaricidal compoundsM Lekimme
Laboratory of Parasitology and Pathology of Parasitic Diseases, Faculty of Veterinary Medicine, University of Liege, Belgium
Med Vet Entomol 20:102-5. 2006..Nevertheless, efficacy of amitraz against both adults and eggs was shown to be higher than that of phoxim... - Comparison of three techniques for the serological diagnosis of Neospora caninum in the dog and their use for epidemiological studiesS Lasri
Laboratory of Parasitology and Pathology of Parasitic Disease, Department of Infectious Parasitic Diseases, Faculty of Veterinary Medicine, University of Liege, Boulevard de Colonster 20, 4000 Liege, Belgium
Vet Parasitol 123:25-32. 2004..This result showed that the neurological symptoms are not usually associated with the Neospora infection. In conclusion, the ELISA developed in our laboratory could replace the IFAT for the screening of a large number of dogs' sera... - Purification and characterization of a 315 kDa keratinolytic subtilisin-like serine protease from Microsporum canis and evidence of its secretion in naturally infected catsB Mignon
Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liege, Belgium
Med Mycol 36:395-404. 1998..The in vitro keratinolytic activity of the enzyme and its production in vivo suggest that it may contribute to pathogenicity... - Pathogenicity and thermotolerance of entomopathogenic fungi for the control of the scab mite, Psoroptes ovisM Lekimme
Laboratory of Parasitology and Pathology of Parasitic Diseases B43, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liege, Boulevard de Colonster 20, 4000, Liege, Belgium
Exp Appl Acarol 46:95-104. 2008..Other critical factors linked with the implementation of this type of biocontrol in P. ovis infected animals are discussed... - The vertical transmission following the reactivation of a Neospora caninum chronic infection does not seem to be due to an alteration of the systemic immune response in pregnant CBA/Ca miceC Rettigner
Laboratory of Parasitology and Pathology of Parasitic Diseases, Faculty of Veterinary Medicine, Department of Infectious and Parasitic Diseases, University of Liege, Boulevard de Colonster, 20, 4000, Liege, Belgium
Parasitology 128:149-60. 2004..caninum chronic infection during pregnancy does not seem to be consecutive to an immunodepression. Nevertheless, pregnancy could favour parasite multiplication in utero after an occasional spontaneous release of bradyzoites... - Purification and characterization of a 43.5 kDa keratinolytic metalloprotease from Microsporum canisF Brouta
Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, , Belgium
Med Mycol 39:269-75. 2001..oryzae, confirming that this 43.5 kDa keratinase is a metalloprotease. This keratinolytic metalloprotease could be a virulence-related factor involved in pathophysiological mechanisms of M. canis dermatophytosis... - Serological and biochemical follow-up in cattle naturally infected with Fasciola hepatica, and comparison with a climate model for predicting risks of fasciolosisK Bossaert
Faculte de Medecine Veterinaire, Universite de Liege, Sart Tilman, Belgium
Vet Res 30:615-28. 1999..The indicators of the infection considered alone were useless and it is concluded that herd diagnosis of fasciolosis may rely on the rise of specific antibody levels, possibly associated with an increase in hepatic enzyme activities... - Humoral immune response in calves to single-dose, trickle and challenge infections with Fasciola hepaticaK Bossaert
Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liege, Sart Tilman, Belgium
Vet Parasitol 87:103-23. 2000..No correlation was found between fluke size and number, and antibody titres, suggesting that IgG1 production has little protective effect against F. hepatica infection... - Oral bioavailability in sheep of albendazole from a suspension and from a solution containing hydroxypropyl-beta-cyclodextrinB Evrard
Lab of Pharmaceutical Technology, Institute of Pharmacy, University of Liege, CHU Tour 4, Bat B36, Avenue de l Hopital 1, 4000 1, Liege, Belgium
J Control Release 85:45-50. 2002..Likewise the peak plasma concentration (C(max)) is twice that of the solution while the time to reach C(max) (T(max)) is reduced... - Cell-mediated immune response in calves to single-dose, trickle, and challenge infections with Fasciola hepaticaK Bossaert
Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liege, Sart Tilman, Belgium
Vet Parasitol 88:17-34. 2000..Liver biopsies were performed and their diagnostic value is discussed. All results suggest that F. hepatica infection predominantly induces a Type-2 response in cattle, and that this response has little protective effect... - Fetal infection with Neospora caninum in dairy and beef cattle in BelgiumF De Meerschman
Laboratory of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, Liege, Belgium
Theriogenology 58:933-45. 2002..001) but no such significant relationships could be demonstrated in dairy fetuses. The study indicated that N. caninum must be considered as an important cause of bovine abortion in Belgium... - Quantification of pigeon circovirus in serum, blood, semen and different tissues of naturally infected pigeons using a real-time polymerase chain reactionJ P Duchatel
Faculty of Veterinary Medicine, Department of Infections and Parasitic Diseases, University of Liege, Liege, Belgium
Avian Pathol 38:143-8. 2009..0 x 10(7) copies/ejaculate) and cloacal swabs (up to 3.6 x 10(10) copies/swab), confirming that PiCV may be transmitted by vertical and horizontal routes... - The carriage of larval echinococcus multilocularis and other cestodes by the musk rat (Ondatra zibethicus) along the Ourthe River and its tributaries (Belgium)A Mathy
Department of Infectious and Parasitic Diseases, University of Liege, 4000 Liege, Belgium
J Wildl Dis 45:279-87. 2009..multilocularis and in Belgium could play the role of reservoir; when present this species could represent an inexpensive and sensitive bioindicator for the study and monitoring of the zoonosis... - Humoral and cellular immune response to a crude exo-antigen and purified keratinase of Microsporum canis in experimentally infected guinea pigsB R Mignon
Faculty of Veterinary Medicine, Department of Parasitology and Parasitic Diseases, University of Liege, Liege, Belgium
Med Mycol 37:123-9. 1999..These results are in agreement with those previously described for naturally infected cats, and indicate that the 31.5 kDa keratinase is not a major antigen in M. canis infection... - Detection of Neospora caninum in dog organs using real time PCR systemsFarida Ghalmi
University of Liege, Faculty of Veterinary Medicine, Department of Infectious Diseases, Parasitology, Sart Tilman, B43, 4000 Liege, Belgium
Vet Parasitol 155:161-7. 2008..Therefore serology can underestimate the real carriage in dogs. However, PCR methods must be improved in terms of sensitivity and inhibition problems... - Distribution of potential bluetongue vectors on Belgium farmsJean-Yves Zimmer
Vet Rec 162:700. 2008 - Immunization and dermatophytesBernard Mignon
Department of Infectious and Parasitic Diseases, Parasitology, Faculty of Veterinary Medicine, University of Liege, Liege, Belgium
Curr Opin Infect Dis 21:134-40. 2008..This review provides an update on the current situation and focuses on recent advances in host-dermatophyte relationships that could have implications for future vaccination against the most prevalent of the fungal diseases... - Dermatitis in a horse associated with the poultry mite (Dermanyssus gallinae)Bernard Mignon
Faculty of Veterinary Medicine, Department of Infectious and Parasitic Diseases, University of Liege, Liege, Belgium
Vet Dermatol 19:38-43. 2008.... - The detection and quantification of a digenean infection in the snail host with special emphasis on Fasciola spYannick Caron
Faculty of Veterinary Medicine, Department of Infectious Diseases, University of Liege, 20 Boulevard de Colonster, B 43a, 4000 Liege, Belgium
Parasitol Res 103:735-44. 2008..However, at the time being this technique is too expensive to be used routinely in the field. The choice between classical or new techniques is usually based on a compromise, as each technique has its advantages and drawbacks... - Survival, immune responses and tissue cyst production in outbred (Swiss white) and inbred (CBA/Ca) strains of mice experimentally infected with Neospora caninum tachyzoitesChantal Rettigner
Laboratory of Parasitology and Pathology of Parasitic Diseases, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liege, Boulevard de Colonster, 20, 4000 Liege, Belgium
Vet Res 35:225-32. 2004..This study indicates that CBA/Ca female mice infected with 5 x 10(6) NC-1 tachyzoites represent a useful model for the study of specific maternal immune responses in pregnant animals... - Use of a serum-free medium to produce in vitro Neospora caninum and Toxoplasma gondii tachyzoites on Vero cellsFrançois De Meerschman
Laboratory of Parasitology and Pathology of Parasitic Diseases, Faculty of Veterinary Medicine, University of Liege, Belgium
Vet Res 33:159-68. 2002..Vero cells, T. gondii and N. caninum tachyzoite production gave similar growth patterns with either HS or DefCell supplemented media. Defcell was considered as a good alternative to supplement culture medium... - Bluetongue in northern EuropeEtienne Thiry
Vet Rec 159:327. 2006 - Ultrastructural morphology of the male and female genital tracts of Psoroptes spp. (Acari: Astigmata: Psoroptidae)Mireille Lekimme
Service of Parasitology and Pathology of Parasitic Diseases, Department of Infectious Diseases, Faculty of Veterinary Medicine, University of Liege, Boulevard de Colonster 20, B43, 4000 Liege, Belgium
Exp Appl Acarol 36:305-24. 2005..Ovaries are composed of somatic cells, germ cells and a central cell, with a multilobular nucleus, connected to oocytes by a stalk. Similarities with other astigmatic mites belonging to Psoroptidia and Acaridia are also discussed... - Secreted subtilisins of Microsporum canis are involved in adherence of arthroconidia to feline corneocytesAline Baldo
Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liege, B43 Sart Tilman, 4000 Liege, Belgium
J Med Microbiol 57:1152-6. 2008..Moreover, two mAbs against the keratinolytic protease subtilisin 3 (Sub3) inhibited M. canis adherence to RFE by 23%, suggesting that subtilisins, and Sub3 in particular, are involved in the adherence process... - RNA silencing in the dermatophyte Microsporum canisSandy Vermout
Department of Infectious and Parasitic Diseases, Parasitology, Faculty of Veterinary Medicine, University of Liege, Liege, Belgium
FEMS Microbiol Lett 275:38-45. 2007..These results show that RNA silencing can be used for functional genomics in M. canis, and particularly to circumvent the limits and technical difficulties of conventional disruption methods... - Biting midges overwintering in BelgiumBertrand Losson
Vet Rec 160:451-2. 2007 - Reconstructed interfollicular feline epidermis as a model for Microsporum canis dermatophytosisJeremy Tabart
Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liege, B43 Sart Tilman, 4000 Liege, Belgium
J Med Microbiol 56:971-5. 2007..In view of these results, an M. canis dermatophytosis model on RFE seems to be a useful tool to investigate mechanisms involved in natural M. canis feline infections... - Breeding sites of bluetongue vectors in northern EuropeJean-Yves Zimmer
Vet Rec 162:131. 2008 - Reconstructed interfollicular feline epidermis as a model for the screening of antifungal drugs against Microsporum canisJeremy Tabart
Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liege, Liege, Belgium
Vet Dermatol 19:130-3. 2008..3 microg mL (final concentration in the culture medium). This study demonstrates that RFE is an adequate model for the in vitro screening of drugs against M. canis and potentially against other skin pathogens...