Research Topics
| R KunertSummaryAffiliation: University of Natural Resources and Applied Life Sciences Country: Austria Publications
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Detail Information
Publications
Characterization of molecular features, antigen-binding, and in vitro properties of IgG and IgM variants of 4E10, an anti-HIV type 1 neutralizing monoclonal antibodyRenate Kunert
Institute of Applied Microbiology, University of Natural Resources and Applied Life Sciences, A 1190 Vienna, Austria
AIDS Res Hum Retroviruses 20:755-62. 2004..The antiviral activity could be greatly enhanced by change of IgG(3) to IgG(1). In contrast, the IgM isotype almost completely lost its neutralizing potential...- Stable recombinant expression of the anti HIV-1 monoclonal antibody 2F5 after IgG3/IgG1 subclass switch in CHO cellsR Kunert
Institute of Applied Microbiology, University of Agricultural Sciences, Muthgasse 18, Haus B, A 1190 Vienna, Austria
Biotechnol Bioeng 67:97-103. 2000..CHO clones have been established, which stably express humAb 2F5 at high levels. The specificity, affinity and in vitro function of both isotypes were identical... - Efficient selection of high-producing subclones during gene amplification of recombinant Chinese hamster ovary cells by flow cytometry and cell sortingN Borth
Institute for Applied Microbiology, University of Agriculture and Forestry, Muthgasse 18, 1190 Vienna, Austria
Biotechnol Bioeng 71:266-73. 2000..4 microM), the clones isolated by sorting were already homogeneous at 0.8 microM methotrexate... - A potent cross-clade neutralizing human monoclonal antibody against a novel epitope on gp41 of human immunodeficiency virus type 1G Stiegler
Institute of Applied Microbiology, University of Agricultural Sciences, A 1190 Vienna, Austria
AIDS Res Hum Retroviruses 17:1757-65. 2001..Moreover, our results suggest that 4E10 should be further investigated for passive anti-HIV immunotherapy... - Analysis of changes during subclone development and ageing of human antibody-producing heterohybridoma cells by northern blot and flow cytometryN Borth
Institute for Applied Microbiology, University of Agriculture, Vienna, Austria
J Biotechnol 67:57-66. 1999..During long time cultivation of the heterohybridoma cell line a continuous decrease in light and heavy chain production was seen without the appearance of a non producing sub-population... - Analysis of alterations in gene expression after amplification of recombinant genes in CHO cellsJ Grillari
Institute of Applied Microbiology, University of Agricultural Sciences, Muthgasse 18, A 1190, Vienna, Austria
J Biotechnol 87:59-65. 2001..Furthermore, an alternatively spliced, non-functional form of the DHFR mRNA was detected, suggesting a dramatic increase of the selection pressure exerted by MTX... - Humanization strategies for an anti-idiotypic antibody mimicking HIV-1 gp41A Mader
Department of Biotechnology, Institute for Applied Microbiology, BOKU University of Natural Resources and Applied Life Sciences, Muthgasse 11, A 1190 Vienna, Austria
Protein Eng Des Sel 23:947-54. 2010..In this study, we developed humanized Ab2/3H6 variants that retained the same affinity as the parental antibody, and are therefore of potential interest for future clinical trails... - Functional analysis of the broadly neutralizing human anti-HIV-1 antibody 2F5 produced in transgenic BY-2 suspension culturesMarkus Sack
Institute of Molecular Biotechnology, RTWH Aachen University, Worringerweg 1, 52074 Aachen, Germany
FASEB J 21:1655-64. 2007..This highlights important issues raised by the use of plant systems to produce human biologics... - Structural analysis and in vivo administration of an anti-idiotypic antibody against mAb 2F5Johannes S Gach
Institute of Applied Microbiology, Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, A 1190 Vienna, Austria
Mol Immunol 45:1027-34. 2008..Moreover, we report about an immunisation study with Ab2/3H6 Fab variants, which elicited a specific 2F5-like humoral immune response in BALB/c mice... - High level expression of a promising anti-idiotypic antibody fragment vaccine against HIV-1 in Pichia pastorisJohannes S Gach
Institute of Applied Microbiology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, A 1190 Vienna, Austria
J Biotechnol 128:735-46. 2007..Biochemical analysis confirmed that the N-glycosylation does not contribute to the binding properties of 3H6... - Protein-free transfection of CHO host cells with an IgG-fusion protein: selection and characterization of stable high producers and comparison to conventionally transfected clonesChristine Lattenmayer
Austrian Center of Biopharmaceutical Technology, Muthgasse 18, 1190 Vienna, Austria
Biotechnol Bioeng 96:1118-26. 2007.... - Biochemical characterization of rhEpo-Fc fusion protein expressed in CHO cellsKornelia Schriebl
Austrian Center of Biopharmaceutical Technology, Muthgasse 18, A 1190 Vienna, Austria
Protein Expr Purif 49:265-75. 2006..The direct comparison with other rhEpo-Fc fusion proteins failed, because no appropriate data were found in the literature... - Process parameter shifting: Part II. Biphasic cultivation-A tool for enhancing the volumetric productivity of batch processes using Epo-Fc expressing CHO cellsEvelyn Trummer
Department of Biotechnology, Institute of Applied Microbiology, University of Natural Resources and Applied Life Sciences Vienna, Muthgasse 18, 1190 Vienna, Austria
Biotechnol Bioeng 94:1045-52. 2006.... - Recombinant antibody 2G12 produced in maize endosperm efficiently neutralizes HIV-1 and contains predominantly single-GlcNAc N-glycansThomas Rademacher
Institute for Molecular Biotechnology, Biology VII, RWTH Aachen, Worringerweg 1, 52074 Aachen, Germany
Plant Biotechnol J 6:189-201. 2008.... - Reassessment of autoreactivity of the broadly neutralizing HIV antibodies 4E10 and 2F5 and retrospective analysis of clinical safety dataBrigitta Vcelar
Polymun Scientific, Vienna bImmunology Outpatient Clinic, Vienna, Austria
AIDS 21:2161-70. 2007..The broadly neutralizing recombinant human HIV-1 antibodies 4E10, 2F5 and Igh1b12 are reported to have autoreactive potential, which is significant for HIV-1 vaccine development and passive immunotherapy using these antibodies... - Production of a monoclonal antibody in plants with a humanized N-glycosylation patternMatthias Schähs
Institute of Applied Genetics and Cell Biology, BOKU Wien, Austria
Plant Biotechnol J 5:657-63. 2007..Our results demonstrate the feasibility of a knockout strategy for N-glycan engineering of plants towards mammalian-like structures, thus providing a significant improvement in the use of plants as an expression platform... - Crystal structure of the complex between the F(ab)' fragment of the cross-neutralizing anti-HIV-1 antibody 2F5 and the F(ab) fragment of its anti-idiotypic antibody 3H6Steve Bryson
Department of Biochemistry, University of Toronto, 1 King s College Circle, Toronto, Ontario, Canada M5S 1A8
J Mol Biol 382:910-9. 2008..This defines Ab2/3H6 as an anti-idiotypic antibody of the Ab2gamma class, i.e., an antigen-inhibitable idiotype that does not carry the internal image of the linear primary gp41 (662)ELDKWAS(668) epitope... - Analysis of immunoglobulin glycosylation by LC-ESI-MS of glycopeptides and oligosaccharidesJohannes Stadlmann
Department of Chemistry, University of Natural Resources and Applied Life Sciences BOKU, Vienna, Austria
Proteomics 8:2858-71. 2008..Regarding fast and yet detailed structural assignment, LC-MS on graphitic carbon supersedes all other current methods... - The broadly neutralizing anti-human immunodeficiency virus type 1 4E10 monoclonal antibody is better adapted to membrane-bound epitope recognition and blocking than 2F5Nerea Huarte
Unidad de Biofisica CSIC UPV EHU, Universidad del Pais Vasco, Aptdo 644, 48080 Bilbao, Spain
J Virol 82:8986-96. 2008..We conclude that the capacity to interfere with the membrane activity of conserved MPER sequences is best correlated with the broad neutralization of the 4E10 MAb... - Transcriptional profiling of phenotypically different Epo-Fc expressing CHO clones by cross-species microarray analysisEvelyn Trummer
Department of Biotechnology, Institute of Applied Microbiology, University of Natural Resources and Applied Life Sciences Vienna, Vienna, Austria
Biotechnol J 3:924-37. 2008..These promising results will further increase our efforts to develop CHO specific microarrays that deliver information about the suitability of a clone candidate for industrial production... - Expression, purification, and in vivo administration of a promising anti-idiotypic HIV-1 vaccineJohannes Simon Gach
Institute of Applied Microbiology, Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, A 1190 Vienna, Austria
Mol Biotechnol 39:119-25. 2008..The crude sera were screened for specific antibodies against the epitope of mAb 2F5 ELDKWA as well as for reactivity against HIV-1 gp41... - A plant-derived human monoclonal antibody induces an anti-carbohydrate immune response in rabbitsChunsheng Jin
Department of Chemistry, University of Natural Resources and Applied Life Sciences, Muthgasse 18, 1190 Vienna, Austria
Glycobiology 18:235-41. 2008..This emphasizes the need for the use of glyco-engineered plants lacking any potentially antigenic N-glycan structures for the production of plant-derived recombinant proteins intended for parenteral human application... - Partial humanization and characterization of an anti-idiotypic antibody against monoclonal antibody 2F5, a potential HIV vaccine?Johannes S Gach
Institute of Applied Microbiology, Department of Biotechnology, University of Natural Resources and Applied Life Sciences, A 1190 Vienna, Austria
AIDS Res Hum Retroviruses 23:1405-15. 2007..Moreover, the inhibition potency of chimeric Ab2/3H6 analyzed by in vitro studies could be shown to be the same as that detected for the hybridoma-derived murine Ab2/3H6... - Process parameter shifting: Part I. Effect of DOT, pH, and temperature on the performance of Epo-Fc expressing CHO cells cultivated in controlled batch bioreactorsEvelyn Trummer
Department of Biotechnology, Institute of Applied Microbiology, University of Natural Resources and Applied Life Sciences Vienna, Muthgasse 18, 1190 Vienna, Austria
Biotechnol Bioeng 94:1033-44. 2006..The well directed alteration of the process environment has emerged as a tool adequate for further process optimization applying a biphasic cultivation strategy... - Broadly neutralizing anti-HIV antibody 4E10 recognizes a helical conformation of a highly conserved fusion-associated motif in gp41Rosa M F Cardoso
Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
Immunity 22:163-73. 2005..These structural insights should assist in the design of immunogens to elicit 4E10-like neutralizing responses... - Anti-human immunodeficiency virus type 1 (HIV-1) antibodies 2F5 and 4E10 require surprisingly few crucial residues in the membrane-proximal external region of glycoprotein gp41 to neutralize HIV-1Michael B Zwick
Department of Immunology IMM 2, The Scripps Research Institute, 10550 North Torrey Pines Rd, La Jolla, CA 92037, USA
J Virol 79:1252-61. 2005..Neutralization experiments showing synergy between and T20 and 4E10 against HIV-1 are also presented. The data presented may aid in the design of antigens that better present the MPER of gp41 to the immune system... - Comprehensive cross-clade neutralization analysis of a panel of anti-human immunodeficiency virus type 1 monoclonal antibodiesJames M Binley
IMM2, Department of Immunology, The Scripps Research Institute, 10550 N Torrey Pines Rd, La Jolla, CA 92037, USA
J Virol 78:13232-52. 2004..As well as the significance for vaccine design, our data have implications for passive-immunization studies in countries where clade C viruses are common, given that only MAbs b12 and 4E10 were effective against viruses from this clade... - Characterization of human class-switched polymeric (immunoglobulin M [IgM] and IgA) anti-human immunodeficiency virus type 1 antibodies 2F5 and 2G12Susanne Wolbank
Institute of Applied Microbiology, University of Agriculture, A-1190 Vienna, Austria
J Virol 77:4095-103. 2003.... - The long third complementarity-determining region of the heavy chain is important in the activity of the broadly neutralizing anti-human immunodeficiency virus type 1 antibody 2F5Michael B Zwick
Department of Immunology, The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, California 92037, USA
J Virol 78:3155-61. 2004..These observations present a challenge to vaccine strategies based on peptide mimics of the linear epitope... - Binding of the 2F5 monoclonal antibody to native and fusion-intermediate forms of human immunodeficiency virus type 1 gp41: implications for fusion-inducing conformational changesEve de Rosny
Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Bethesda, Maryland 20892-4555, USA
J Virol 78:2627-31. 2004..We also demonstrate conformational changes in the C heptad of gp41... - Antibody domain exchange is an immunological solution to carbohydrate cluster recognitionDaniel A Calarese
Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
Science 300:2065-71. 2003..The unique interdigitation of Fab domains within an antibody uncovers a previously unappreciated mechanism for high-affinity recognition of carbohydrate or other repeating epitopes on cell or microbial surfaces... - Cardiolipin polyspecific autoreactivity in two broadly neutralizing HIV-1 antibodiesBarton F Haynes
Duke University School of Medicine, Durham, NC 27710, USA
Science 308:1906-8. 2005..These results may have important implications for generating effective neutralizing antibody responses by using HIV-1 vaccines... - Antiviral activity of the neutralizing antibodies 2F5 and 2G12 in asymptomatic HIV-1-infected humans: a phase I evaluationGabriela Stiegler
Institute of Applied Microbiology, University of Agricultural Sciences, Muthgasse, Vienna, Austria
AIDS 16:2019-25. 2002..However, the antiviral effects of antibody treatment have not been fully analyzed in this first clinical trial... - Effect of increased expression of protein disulfide isomerase and heavy chain binding protein on antibody secretion in a recombinant CHO cell lineNicole Borth
Institute for Applied Microbiology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, 1190 Vienna, Austria
Biotechnol Prog 21:106-11. 2005..Overexpression of BIP, both alone and in combination with PDI, reduced the specific secretion rate, whereas PDI, when overexpressed alone, caused an increase of product secretion rate... - Anti-neuroblastoma effect of ch14.18 antibody produced in CHO cells is mediated by NK-cells in miceYan Zeng
, University Medicine, 13353 Berlin, Germany
Mol Immunol 42:1311-9. 2005..In vivo depletion of NK-cells completely abrogated this effect, suggesting that the mechanism involved in the ch14.18/CHO induced anti-neuroblastoma effect is mediated by NK-dependent ADCC... - HIV-1 mutants escaping neutralization by the human antibodies 2F5, 2G12, and 4E10: in vitro experiments versus clinical studiesSabine Nakowitsch
Institute of Applied Microbiology, University of Natural Resources and Applied Life Sciences, Vienna, Austria
AIDS 19:1957-66. 2005.... - Validated method for quantification of genetically modified organisms in samples of maize flourRenate Kunert
Institute of Applied Microbiology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, A 1190 Vienna, Austria
J Agric Food Chem 54:678-81. 2006..The results were evaluated with ValiData for homology of variance, linearity, accuracy of the standard curve, and standard deviation... - Engineering of Pichia pastoris for improved production of antibody fragmentsBrigitte Gasser
Institute of Applied Microbiology, BOKU University of Natural Resources and Applied Life Sciences Vienna, Muthgasse 18, A-1190 Vienna, Austria
Biotechnol Bioeng 94:353-61. 2006..3-fold, PDI enabled an increase of the Fab level by 1.9-fold. Hence, the formation of interchain disulfide bonds can be seen as a major rate limiting factor to Fab assembly and subsequent secretion... - Inhibition of human immunodeficiency virus type 1 entry in cells expressing gp41-derived peptidesMarc Egelhofer
Institute for Biomedical Research Georg-Speyer-Haus, Frankfurt a. M. Medizinische Hochschule Hannover, Hannover, Germany
J Virol 78:568-75. 2004..Preclinical toxicity and efficacy studies of this antiviral vector have been completed, and clinical trials are in preparation... - A novel strategy for quantitative isoform detection directly performed from culture supernatantKornelia Schriebl
Austrian Center of Biopharmaceutical Technology, Muthgasse 18, A 1190 Vienna, Austria
J Pharm Biomed Anal 42:322-7. 2006..A fundamental benefit of this technique is the ability to perform quantitative isoform pattern analysis directly from serum-free culture supernatant... - Electron microscopic and immunochemical analysis of the broadly neutralizing HIV-1-specific, anti-carbohydrate antibody, 2G12K H Roux
Department of Biological Science, 2nd Institute of Molecular Biophysics, Florida State University, Tallahassee, FL 32306 4307, USA
Mol Immunol 41:1001-11. 2004..Efforts to identify IgG molecules with a similar intertwined Fab dimer structure in a large IgG pool were unsuccessful... - A peptide inhibitor of HIV-1 neutralizing antibody 2G12 is not a structural mimic of the natural carbohydrate epitope on gp120Alfredo Menendez
Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC, V5A 1S6, Canada
FASEB J 22:1380-92. 2008..Moreover, 2G12.1 peptide is not an immunogenic mimic of the 2G12 epitope, since antisera produced against it did not bind gp120... - Recognition and blocking of HIV-1 gp41 pre-transmembrane sequence by monoclonal 4E10 antibody in a Raft-like membrane environmentMaier Lorizate
, University of the Basque Country, P. O. Box 644, 48080 Bilbao, Spain
J Biol Chem 281:39598-606. 2006..This work comprises the first experimental report on a lipid-modulated antibody capacity to bind a membrane-bound epitope sequence and arrest its restructuring activity... - Characterisation of recombinant CHO cell lines by investigation of protein productivities and genetic parametersChristine Lattenmayer
Austrian Center of Biopharmaceutical Technology, Muthgasse 18, 1190 Vienna, Austria
J Biotechnol 128:716-25. 2007..Nevertheless, a wider range of cell lines has to be investigated in order to implement genetic analyses into a screening process... - Applicability of different fluorescent dyes for isoform quantification on linear IPG gelsKornelia Schriebl
Austrian Center of Biopharmaceutical Technology, Vienna, Austria
Electrophoresis 28:2100-7. 2007..g., clone screening, process optimization, and purification monitoring... - Improvement of the energy metabolism of recombinant CHO cells by cell sorting for reduced mitochondrial membrane potentialGeorg Hinterkörner
Institute for Applied Microbiology, Department of Biotechnology, Universität für Bodenkultur, Muthgasse 18, Vienna, Austria
J Biotechnol 129:651-7. 2007..The potential of using flow cytometric cell sorting methods based on physiological activity for cell line optimisation is discussed...