Research Topics
Genomes and GenesSpecies | Juraj GreganSummaryAffiliation: University of Vienna Country: Austria Publications
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Publications
Construction of conditional analog-sensitive kinase alleles in the fission yeast Schizosaccharomyces pombeJuraj Gregan
Department of Chromosome Biology, Max F Perutz Laboratories, University of Vienna, Dr Bohr Gasse 1, 1030 Vienna, Austria
Nat Protoc 2:2996-3000. 2007..Here, we describe a protocol to construct conditional analog-sensitive kinase alleles in the fission yeast Schizosaccharomyces pombe. This protocol can be completed in about 3 weeks and should be applicable to other organisms as well...- Chemogenomic and transcriptome analysis identifies mode of action of the chemosensitizing agent CTBT (7-chlorotetrazolo[5,1-c]benzo[1,2,4]triazine)Monika Batova
Comenius University in Bratislava, Department of Microbiology and Virology, Bratislava, Slovak Republic
BMC Genomics 11:153. 2010..Here we elucidate CTBT mode of action with a combination of systematic genetic and transcriptome analysis... - The kinetochore proteins Pcs1 and Mde4 and heterochromatin are required to prevent merotelic orientationJuraj Gregan
Max F Perutz Laboratories, Department of Chromosome Biology, University of Vienna, Dr Bohr Gasse 1, 1030 Vienna, Austria
Curr Biol 17:1190-200. 2007..A protein called Pcs1 has been implicated in preventing merotelic attachment in mitosis and meiosis II in the fission yeast S. pombe... - Solving the shugoshin puzzleJuraj Gregan
Max F Perutz Laboratories, University of Vienna, 1030 Vienna, Austria
Trends Genet 24:205-7. 2008.... - What makes centromeric cohesion resistant to separase cleavage during meiosis I but not during meiosis II?Juraj Gregan
Max F Perutz Laboratories, Department of Chromosome Biology, University of Vienna, Vienna, Austria
Cell Cycle 7:151-3. 2008.... - Tandem affinity purification of functional TAP-tagged proteins from human cellsJuraj Gregan
Department of Chromosome Biology, Max F Perutz Laboratories, University of Vienna, Dr Bohr Gasse 1, 1030 Vienna, Austria
Nat Protoc 2:1145-51. 2007..The purification protocol can be completed in 1 day and it should be applicable to other proteins... - High-throughput knockout screen in fission yeastJuraj Gregan
Research Institute of Molecular Pathology, Dr Bohr Gasse 7, 1030 Vienna, Austria
Nat Protoc 1:2457-64. 2006..Here, we present our protocol with detailed instructions. Using this protocol, one person can knock out 96 S. pombe genes in 8 days... - Merotelic kinetochore attachment: causes and effectsJuraj Gregan
Max F Perutz Laboratories, University of Vienna, Dr Bohr Gasse 1, 1030 Vienna, Austria
Trends Cell Biol 21:374-81. 2011..Here, we highlight recent progress in our understanding of how cells prevent and correct merotelic kinetochore attachments... - Protein phosphatase 2A protects centromeric sister chromatid cohesion during meiosis IChristian G Riedel
Research Institute of Molecular Pathology IMP, Dr Bohr Gasse 7, A 1030 Vienna, Austria
Nature 441:53-61. 2006..Our data are consistent with the notion that efficient cleavage of Rec8 requires phosphorylation of cohesin and that this is blocked by PP2A at meiosis I centromeres... - Monopolar attachment of sister kinetochores at meiosis I requires casein kinase 1Mark Petronczki
Research Institute of Molecular Pathology, Dr Bohrgasse 7, A 1030 Vienna, Austria
Cell 126:1049-64. 2006.... - Laser microsurgery provides evidence for merotelic kinetochore attachments in fission yeast cells lacking Pcs1 or Clr4Cornelia Rumpf
Max F Perutz Laboratories, University of Vienna, Vienna, Austria
Cell Cycle 9:3997-4004. 2010..Finally, we show that Pcs1/Mde4 complex shares similar features with the conserved kinetochore complex Spc24/Spc25 suggesting that these two complexes may occupy a similar functional niche... - Casein kinase 1 is required for efficient removal of Rec8 during meiosis ICornelia Rumpf
Max F Perutz Laboratories, University of Vienna, Vienna, Austria
Cell Cycle 9:2657-62. 2010..Our data are consistent with the model that Hhp1/Hhp2-dependent phosphorylation of Rec8 is required for separase-mediated cleavage of Rec8 during meiosis I... - Generation of a set of conditional analog-sensitive alleles of essential protein kinases in the fission yeast Schizosaccharomyces pombeLubos Cipak
Max F Perutz Laboratories, University of Vienna, Vienna, Austria
Cell Cycle 10:3527-32. 2011..Using this approach, we constructed conditional analog-sensitive alleles of 13 essential protein kinases in the fission yeast S. pombe... - Chromosome segregation: monopolin attracts condensinAndrej Dudas
Max F Perutz Laboratories, University of Vienna, Dr Bohr Gasse 1, 1030 Vienna, Austria
Curr Biol 21:R634-6. 2011..Recent evidence suggests that cooperation between Pcs1/Mde4 and condensin complexes plays an important role in preventing merotely... - Maintenance of cohesin at centromeres after meiosis I in budding yeast requires a kinetochore-associated protein related to MEI-S332Vittorio L Katis
Research Institute of Molecular Pathology, Dr Bohrgasse 7, A 1030 Vienna, Austria
Curr Biol 14:560-72. 2004..Third, cohesion between sister centromeres must persist after the first meiotic division to enable their biorientation at the second... - Novel genes required for meiotic chromosome segregation are identified by a high-throughput knockout screen in fission yeastJuraj Gregan
Research Institute of Molecular Pathology, Dr Bohr Gasse 7, A 1030 Vienna, Austria
Curr Biol 15:1663-9. 2005..sgo1 is required for retention of centromeric sister-chromatid cohesion after anaphase I. We show here that mde2 is required for formation of the double-strand breaks necessary for meiotic recombination... - High-throughput knockout screen in Schizosaccharomyces pombe identifies a novel gene required for efficient homolog disjunction during meiosis ICornelia Rumpf
Max F Perutz Laboratories, Department of Chromosome Biology, University of Vienna, Vienna, Austria
Cell Cycle 9:1802-8. 2010..Using this approach, we identified a new protein, Dil1, which is required to prevent meiosis I homolog non-disjunction. We show that Dil1 acts in the dynein pathway to promote oscillatory nuclear movement during meiosis... - Two fission yeast homologs of Drosophila Mei-S332 are required for chromosome segregation during meiosis I and IIKirsten P Rabitsch
Research Institute of Molecular Pathology, Dr Bohr Gasse 7, A 1030 Vienna, Austria
Curr Biol 14:287-301. 2004..Segregation during the second division depends on retention of sister chromatid cohesion between centromeres until the onset of anaphase II, which in Drosophila melanogaster depends on a protein called Mei-S332 that binds to centromeres... - Sgo1 is required for co-segregation of sister chromatids during achiasmate meiosis IAndrej Dudas
Department of Chromosome Biology, Max F Perutz Laboratories, University of Vienna, Austria
Cell Cycle 10:951-5. 2011..Our data are consistent with the notion that sister kinetochores are frequently bi-oriented in the absence of chiasmata and that Sgo1 prevents equational segregation of sister chromatids during achiasmate meiosis I... - An improved strategy for tandem affinity purification-tagging of Schizosaccharomyces pombe genesLubos Cipak
Max F Perutz Laboratories, University of Vienna, Vienna, Austria
Proteomics 9:4825-8. 2009..imp.ac.at/Pombe_tagging/). To validate the approach, we purified the proteins, which associated with two evolutionarily conserved proteins Swi5 and Sfr1 as well as three protein kinases Ksg1, Orb6 and Sid1... - S. pombe genome deletion project: an updateMario Spirek
Max F Perutz Laboratories, University of Vienna, Vienna, Austria
Cell Cycle 9:2399-402. 2010..The collection of deletion mutants now covers 99% of the fission yeast open reading frames... - How might DNA enter the cohesin ring?Juraj Gregan
Max F. Perutz Laboratories, University of Vienna, Vienna, Austria. juraj.gregan@ univie.ac.at
Cell Cycle 5:2553-4. 2006 - Is chromatin remodeling required to build sister-chromatid cohesion?Christian G Riedel
Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria
Trends Biochem Sci 29:389-92. 2004..It remains unclear whether they do so by facilitating the recruitment of cohesin to specific chromosomal sequences or by modifying an event at replication forks giving rise to cohesion between sister DNAs... - Microtubule organization: a pericentriolar material-like structure in yeast meiosisAlexander Dammermann
Max F Perutz Laboratories, University of Vienna, Dr Bohr Gasse 1, 1030 Vienna, Austria
Curr Biol 22:R229-31. 2012..A newly identified structure, the radial microtubule organizing center (rMTOC), mediates these movements and shares some of the features of the pericentriolar material in higher eukaryotes... - Control of Shugoshin function during fission-yeast meiosisSabine Vaur
Centre National de la Recherche Scientifique, Institut de Biochimie et Génétique Cellulaires, Unité Mixte de Recherche 5095, Bordeaux, F 33077, France
Curr Biol 15:2263-70. 2005..Sgo1-mediated protection of centromere cohesion might therefore be regulated by the mode of kinetochore attachment... - Nuclear distribution and chromatin association of DNA polymerase alpha-primase is affected by TEV protease cleavage of Cdc23 (Mcm10) in fission yeastXiaowen Yang
Department of Zoology, University of Oxford, South Parks Road, Oxford OX13PS, UK
BMC Mol Biol 6:13. 2005..Cdc23/Mcm10 is required for the initiation and elongation steps of DNA replication but its biochemical function is unclear. Here, we probe its function using a novel approach in fission yeast, involving Cdc23 cleavage by the TEV protease... - A yeast model for the study of human DFNA5, a gene mutated in nonsyndromic hearing impairmentJuraj Gregan
Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, UK
Biochim Biophys Acta 1638:179-86. 2003..Genetic interactions between heterologously expressed DFNA5 and a fission yeast cdc23 (mcm10) mutant support a possible link between DFNA5 and Mcm10 proteins... - Conditional alleles, inteins and degronsJuraj Gregan
Nat Biotechnol 23:18. 2005 - Fission yeast Cdc23/Mcm10 functions after pre-replicative complex formation to promote Cdc45 chromatin bindingJuraj Gregan
Department of Zoology, University of Oxford, Oxford, OX1 3PS, United Kingdom
Mol Biol Cell 14:3876-87. 2003..On return to growth, Cdc23 chromatin binding is rapidly reestablished independently from pre-RC formation, suggesting that chromatin association of Cdc23 provides a link between proliferation and competence to execute DNA replication...