Andrea Buchacher

Summary

Affiliation: Pre-Clinical Research and Development
Country: Austria

Publications

  1. ncbi Continuous removal of protein aggregates by annular chromatography
    A Buchacher
    Octapharma Pharmazeutische ProduktionsGes m b H, Oberlaaerstrasse 235, A 1100 Vienna, Austria
    Biotechnol Prog 17:140-9. 2001
  2. ncbi Purification of intravenous immunoglobulin G from human plasma--aspects of yield and virus safety
    Andrea Buchacher
    Octapharma Pharmazeutika Produktions GmbH, Oberlaaerstrasse 235, 1100 Vienna, Austria
    Biotechnol J 1:148-63. 2006
  3. ncbi Anticomplementary activity of IVIG concentrates--important assay parameters and impact of IgG polymers
    A Buchacher
    Octapharma Pharmazeutika ProduktionsGmbH, Vienna, Austria
    Vox Sang 98:e209-18. 2010
  4. ncbi Preparation of vitamin K-dependent proteins, such as clotting factors II, VII, IX and X and clotting inhibitor protein C
    Djuro Josic
    Octapharma Pharmazeutika Produktions Ges m b H, Oberlaaerstrasse 235, A 1100, Vienna, Austria
    J Chromatogr B Analyt Technol Biomed Life Sci 790:183-97. 2003
  5. ncbi Separation of active and inactive forms of human antithrombin by heparin affinity chromatography
    Andrea Heger
    Research and Development, Octapharma Pharmazeutika Produktionsges m b H, Oberlaaer Strasse 235, A 1100 Vienna, Austria
    Thromb Res 106:157-64. 2002
  6. ncbi Characterization of antithrombin III from human plasma by two-dimensional gel electrophoresis and capillary electrophoretic methods
    Leopold Kremser
    Institute of Analytical Chemistry, University of Vienna, Vienna, Austria
    Electrophoresis 24:4282-90. 2003
  7. ncbi Isolation of esterified fatty acids bound to serum albumin purified from human plasma and characterised by MALDI mass spectrometry
    Omar Belgacem
    Octapharma Pharmazeutika, Oberlaaer Str 235, A 1100 Vienna, Austria
    Biologicals 35:43-9. 2007
  8. ncbi Molecular mass determination of plasma-derived glycoproteins by ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry with internal calibration
    Omar Belgacem
    Institute for Analytical Chemistry, University of Vienna, , A-1090 Vienna, Austria
    J Mass Spectrom 37:1118-30. 2002
  9. ncbi Exact molecular mass determination of various forms of native and de-N-glycosylated human plasma-derived antithrombin by means of electrospray ionization ion trap mass spectrometry
    Martina Kleinova
    Institute of Chemical Technologies and Analysis, Vienna University of Technology, Getreidemarkt 9/164, A-1060 Vienna, Austria
    J Mass Spectrom 39:1429-36. 2004

Collaborators

Detail Information

Publications10

  1. ncbi Continuous removal of protein aggregates by annular chromatography
    A Buchacher
    Octapharma Pharmazeutische ProduktionsGes m b H, Oberlaaerstrasse 235, A 1100 Vienna, Austria
    Biotechnol Prog 17:140-9. 2001
    ..We have demonstrated that size exclusion can be performed on an industrial scale when it is run continuously with the aid of a pressurized annular chromatograph...
  2. ncbi Purification of intravenous immunoglobulin G from human plasma--aspects of yield and virus safety
    Andrea Buchacher
    Octapharma Pharmazeutika Produktions GmbH, Oberlaaerstrasse 235, 1100 Vienna, Austria
    Biotechnol J 1:148-63. 2006
    ..Some of these methods also have the ability to separate prions. High pathogen safety and high yields have become the dominant goals of the plasma fractionation industry...
  3. ncbi Anticomplementary activity of IVIG concentrates--important assay parameters and impact of IgG polymers
    A Buchacher
    Octapharma Pharmazeutika ProduktionsGmbH, Vienna, Austria
    Vox Sang 98:e209-18. 2010
    ..The goal of this study was to scrutinize critical parameters of the ACA assay and the influence of different polymer variants of IgG on the complement consumption...
  4. ncbi Preparation of vitamin K-dependent proteins, such as clotting factors II, VII, IX and X and clotting inhibitor protein C
    Djuro Josic
    Octapharma Pharmazeutika Produktions Ges m b H, Oberlaaerstrasse 235, A 1100, Vienna, Austria
    J Chromatogr B Analyt Technol Biomed Life Sci 790:183-97. 2003
    ..The methods for their purification are described. The problem of difference in posttranslational modifications between plasma derived and recombinant protein is discussed with regard to therapeutic proteins...
  5. ncbi Separation of active and inactive forms of human antithrombin by heparin affinity chromatography
    Andrea Heger
    Research and Development, Octapharma Pharmazeutika Produktionsges m b H, Oberlaaer Strasse 235, A 1100 Vienna, Austria
    Thromb Res 106:157-64. 2002
    ..In four commercially available antithrombin preparations, all produced by pasteurization, the amount of inactive protein varied between 0.5% and 9.5%...
  6. ncbi Characterization of antithrombin III from human plasma by two-dimensional gel electrophoresis and capillary electrophoretic methods
    Leopold Kremser
    Institute of Analytical Chemistry, University of Vienna, Vienna, Austria
    Electrophoresis 24:4282-90. 2003
    ..6% of ATIII-beta. The pI values of ATIII determined by CIEF with internal calibration were in fair agreement with the pI values of the main isoforms achieved with 2-DE...
  7. ncbi Isolation of esterified fatty acids bound to serum albumin purified from human plasma and characterised by MALDI mass spectrometry
    Omar Belgacem
    Octapharma Pharmazeutika, Oberlaaer Str 235, A 1100 Vienna, Austria
    Biologicals 35:43-9. 2007
    ..Besides the native ligands from plasma a triacylglycerol was detected that has been added during the albumin preparation steps...
  8. ncbi Molecular mass determination of plasma-derived glycoproteins by ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry with internal calibration
    Omar Belgacem
    Institute for Analytical Chemistry, University of Vienna, , A-1090 Vienna, Austria
    J Mass Spectrom 37:1118-30. 2002
    ..41 kDa, corresponding to V65 and V10 subunits of VN. PNGase F digest of the V75 and V65 units and MS analysis, exhibiting a molecular mass reduction of 6.37 kDa in both cases, verified the presence of a considerable amount of N-glycans...
  9. ncbi Exact molecular mass determination of various forms of native and de-N-glycosylated human plasma-derived antithrombin by means of electrospray ionization ion trap mass spectrometry
    Martina Kleinova
    Institute of Chemical Technologies and Analysis, Vienna University of Technology, Getreidemarkt 9/164, A-1060 Vienna, Austria
    J Mass Spectrom 39:1429-36. 2004
    ..The determined molecular masses after reduction/alkylation and de-N-glycosylation of the alpha-and beta-forms are almost equal, indicating that no major differences exist between the three preparations on the amino acid level...