Roger John TruscottSummaryAffiliation: University of Wollongong Country: Australia Publications
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Publications
Post-translational modifications in the nuclear region of young, aged, and cataract human lensesPeter G Hains
Save Sight Institute, University of Sydney, Sydney, NSW, 2001, Australia
J Proteome Res 6:3935-43. 2007..These findings suggest Trp oxidation may be involved in nuclear cataract development...
Age-related nuclear cataract-oxidation is the keyRoger J W Truscott
Australian Cataract Research Foundation, University of Wollongong, Northfields Ave, Wollongong, NSW 2522, Australia
Exp Eye Res 80:709-25. 2005..Unfortunately, due to marked variability in the lenses of different species, there appears at present to be no ideal animal model system for studying human ARN cataract...
Are ancient proteins responsible for the age-related decline in health and fitness?Roger John Willis Truscott
Save Sight Institute, University of Sydney, Sydney, NSW, Australia
Rejuvenation Res 13:83-9. 2010..The human lens may be particularly useful for discovering which reactive metabolites in the body are of most importance for posttranslational modification of long-lived proteins...
Presbyopia. Emerging from a blur towards an understanding of the molecular basis for this most common eye conditionRoger John Truscott
Save Sight Institute, University of Sydney, NSW 2001, Australia
Exp Eye Res 88:241-7. 2009..Understanding such processes may allow us to glimpse the origin both of presbyopia and age-related nuclear cataract...
Protein-bound and free UV filters in cataract lenses. The concentration of UV filters is much lower than in normal lensesAnastasia Korlimbinis
Save Sight Institute, University of Sydney, GPO Box 4337, Sydney, NSW 2001, Australia
Exp Eye Res 85:219-25. 2007....
Protein-bound UV filters in normal human lenses: the concentration of bound UV filters equals that of free UV filters in the center of older lensesAnastasia Korlimbinis
Save Sight Institute, University of Sydney, NSW 2001, Sydney, Australia
Invest Ophthalmol Vis Sci 48:1718-23. 2007..To survey the levels of protein-bound UV filters in the cortices and nuclei of normal human lenses as a function of age and to relate this to the concentration of free UV filters...
Large-scale binding of ?-crystallin to cell membranes of aged normal human lenses: a phenomenon that can be induced by mild thermal stressMichael G Friedrich
Save Sight Institute, Sydney University, Sydney, Australia
Invest Ophthalmol Vis Sci 51:5145-52. 2010..This study focused on membrane binding within the barrier region and the outermost lens cortex...
Tryptophan-derived ultraviolet filter compounds covalently bound to lens proteins are photosensitizers of oxidative damageJasminka Mizdrak
Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW 2109, Australia
Free Radic Biol Med 44:1108-19. 2008..These data indicate that the covalent modification of lens proteins by Kyn derivatives yields photosensitizers that may enhance oxidation in older lenses and contribute to age-related nuclear cataract...
Identification of 3-hydroxykynurenine bound to proteins in the human lens. A possible role in age-related nuclear cataractAnastasia Korlimbinis
Australian Cataract Research Foundation, University of Wollongong, New South Wales 2522, Australia
Biochemistry 45:1950-60. 2006..In agreement with this hypothesis, no bound 3OHKyn could be detected in proteins isolated from ARN cataract lenses...
Protein-bound kynurenine decreases with the progression of age-related nuclear cataractSantiago Vazquez
Australian Cataract Research Foundation, University of Wollongong, Wollongong, New South Wales, Australia
Invest Ophthalmol Vis Sci 45:879-83. 2004..This finding suggests that in cataract there is a breakdown of the protein-bound adducts. Such further reactions of bound UV filters may contribute to the etiology of age-related nuclear cataract...
Proteomic analysis of the oxidation of cysteine residues in human age-related nuclear cataract lensesPeter G Hains
Save Sight Institute, University of Sydney, Sydney, NSW 2001, Australia
Biochim Biophys Acta 1784:1959-64. 2008..The vast majority of the loss of protein thiol observed in such lenses is due to disulphide bond formation...
3-Hydroxykynurenine oxidizes alpha-crystallin: potential role in cataractogenesisAnastasia Korlimbinis
Save Sight Institute, Sydney, NSW 2001, Australia
Biochemistry 45:1852-60. 2006..Thus, 3-hydroxykynurenine promotes the oxidation and modification of crystallins and may contribute to oxidative stress in the human lens...
Reversible binding of kynurenine to lens proteins: potential protection by glutathione in young lensesNicole R Parker
Save Sight Institute, University of Sydney, Sydney, NSW, Australia
Invest Ophthalmol Vis Sci 48:3705-13. 2007..The aim of this study was to examine in vitro rates of formation and decomposition of the three major Kyn-amino acid adducts and possible consequences for the lens...
Is hypochlorous acid (HOCl) involved in age-related nuclear cataract?Linda J Hazell
The Heart Research Institute, University of Wollongong, NSW, Australia
Clin Exp Optom 85:97-100. 2002..CONCLUSIONS: On the basis of this study, no evidence was obtained to support a role for HOCl in age-related nuclear cataract...
Age-related nuclear cataract and indoleamine 2,3-dioxygenase-initiated tryptophan metabolism in the human lensOsamu Takikawa
Australian Cataract Research Foundation, University of Wollongong, NSW 2522, Australia
Adv Exp Med Biol 527:277-85. 2003..Yellowing of the aged lens may therefore be preventable by drug-induced suppression of IDO activity...
Membrane association of proteins in the aging human lens: profound changes take place in the fifth decade of lifeMichael G Friedrich
Save Sight Institute, Sydney University, Sydney, NSW, Australia
Invest Ophthalmol Vis Sci 50:4786-93. 2009..To characterize age-related changes to proteins in the center of the human lens...
Phospholipid composition of the rat lens is independent of dietJessica R Nealon
School of Chemistry, University of Wollongong, Wollongong, 2522 NSW, Australia
Exp Eye Res 87:502-14. 2008..As phospholipids determine membrane fluidity and influence the activity and function of integral membrane proteins, regulation of their composition may be important for the function of the lens...
Age-dependent deamidation of lifelong proteins in the human lensPeter G Hains
Save Sight Institute, University of Sydney, Sydney, NSW, Australia
Invest Ophthalmol Vis Sci 51:3107-14. 2010..The aim of this study was to identify the sites of deamidation in older lenses...
Lenticular levels of amino acids and free UV filters differ significantly between normals and cataract patientsIsla M Streete
Australian Cataract Research Foundation, University of Wollongong, Wollongong, New South Wales, Australia
Invest Ophthalmol Vis Sci 45:4091-8. 2004..Because kynurenine levels in cataract were not significantly different from those of normal lenses, there may be a defect in the lenticular UV filter pathway at one, or both, of the steps that convert kynurenine to 3OHKG...
Novel protein modification by kynurenine in human lensesSantiago Vazquez
Australian Cataract Research Foundation and Department of Chemistry, University of Wollongong, New South Wales 2522, Australia
J Biol Chem 277:4867-73. 2002....
Free and bound water in normal and cataractous human lensesKarl R Heys
Save Sight Institute, Sydney University, Sydney, New South Wales 2001, Australia
Invest Ophthalmol Vis Sci 49:1991-7. 2008..To analyze free and total water in human normal and cataractous lenses...
Asp274 and his346 are essential for heme binding and catalytic function of human indoleamine 2,3-dioxygenaseTamantha K Littlejohn
Australian Cataract Research Foundation, Department of Chemistry, University of Wollongong, New South Wales, Australia
J Biol Chem 278:29525-31. 2003..This observation indicates that Asp274 may coordinate to heme directly as the distal ligand or is essential in maintaining the conformation of the heme pocket...
Inhibition of indoleamine 2,3 dioxygenase activity by H2O2Anne Poljak
Bioanalytical Mass Spectrometry Facility, University of New South Wales, Sydney, Australia
Arch Biochem Biophys 450:9-19. 2006..We present evidence that cysteine oxidation represents one mechanism of indoleamine 2,3-dioxygenase inhibition...
Glutathione and NADH, but not ascorbate, protect lens proteins from modification by UV filtersLisa M Taylor
Australian Cataract Research Foundation, University of Wollongong, Wollongong, NSW, Australia 2522
Exp Eye Res 74:503-11. 2002..Since GSH is present at far greater concentrations than NADH, these results show that in primate lenses, GSH is the key agent responsible for protecting the crystallins from covalent modification...
UV filter instability: consequences for the human lensLisa M Taylor
Australian Cataract Research Foundation, University of Wollongong, Wollongong, N.S.W. 2522, Australia
Exp Eye Res 75:165-75. 2002..On the basis of these data it is likely that glutathione and NAD(P)H, but not ascorbate, protect proteins in the lens from modification by UV filters...
Protein-bound kynurenine is a photosensitizer of oxidative damageNicole R Parker
Australian Cataract Research Foundation, Department of Chemistry, University of Wollongong, Wollongong, NSW 2522, Australia
Free Radic Biol Med 37:1479-89. 2004....
Protein aging: truncation of aquaporin 0 in human lens regions is a continuous age-dependent processAnastasia Korlimbinis
Save Sight Institute, University of Sydney, NSW, Australia
Exp Eye Res 88:966-73. 2009..Once a significant proportion of AQP0 has been cleaved, this occlusion may contribute to the formation of the lens permeability barrier that develops at middle age...
Identification of the new UV filter compound cysteine-L-3-hydroxykynurenine O-beta-d-glucoside in human lensesPeter G Hains
Save Sight Institute, University of Sydney, Sydney, NSW 2001, Australia
FEBS Lett 580:5071-6. 2006..Quantitation studies revealed considerable variation in normal lens levels that may be traced to the marked instability of the cysteine adduct. The novel UV filter was not detected in advanced nuclear cataract lenses...
Presbyopia and heat: changes associated with aging of the human lens suggest a functional role for the small heat shock protein, alpha-crystallin, in maintaining lens flexibilityKarl R Heys
Save Sight Institute, University of Sydney, Sydney, NSW 2001, Australia
Aging Cell 6:807-15. 2007..Presbyopia may be the result of a loss of alpha-crystallin coupled with progressive heat-induced denaturation of structural proteins in the lens during the first five decades of life...
The photosensitiser xanthurenic acid is not present in normal human lensesPeter G Hains
Australian Cataract Research Foundation, University of Wollongong, Wollongong, NSW 2522, Australia
Exp Eye Res 77:547-53. 2003..Therefore, it appears unlikely that xanthurenic acid plays a role in lens aging or human cataract...
The stiffness of human cataract lenses is a function of both age and the type of cataractKarl R Heys
Exp Eye Res 86:701-3. 2008..5...
