Research Topics
Genomes and GenesSpecies | Richard B ToddSummaryAffiliation: University of Melbourne Country: Australia Publications
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Publications
Nuclear accumulation of the GATA factor AreA in response to complete nitrogen starvation by regulation of nuclear exportRichard B Todd
Department of Genetics, The University of Melbourne, Victoria 3010, Australia
Eukaryot Cell 4:1646-53. 2005..Nitrogen source availability, but not quality, affects nuclear accumulation by regulating nuclear exit of AreA, providing a rapid response to changes in the supply of nutrients...
Genetic manipulation of Aspergillus nidulans: meiotic progeny for genetic analysis and strain constructionRichard B Todd
Department of Genetics, The University of Melbourne, Parkville, Victoria 3010, Australia
Nat Protoc 2:811-21. 2007..Meiotic crossing is used for construction of multiple mutant strains for genetic analysis. Genetic crossing and analysis of progeny can be undertaken in 2-3 weeks and growth testing takes 2-3 days...
Genetic manipulation of Aspergillus nidulans: heterokaryons and diploids for dominance, complementation and haploidization analysesRichard B Todd
Department of Genetics, The University of Melbourne, Parkville, Victoria 3010, Australia
Nat Protoc 2:822-30. 2007..Haploidization analysis of heterozygous diploids can be achieved within 10 days. These protocols should be adaptable for use in related Aspergilli and Penicillia, which lack a known meiotic cycle...
The Aspergillus nidulans rcoA gene is required for veA-dependent sexual developmentRichard B Todd
Department of Genetics, University of Melbourne, Parkville, Victoria 3010, Australia
Genetics 174:1685-8. 2006..Furthermore, sexual development resulting from veA overexpression is rcoA dependent, indicating that rcoA lies downstream of veA in the sexual development pathway...
Deletion and overexpression of the Aspergillus nidulans GATA factor AreB reveals unexpected pleiotropyKoon Ho Wong
Department of Genetics, The University of Melbourne, Parkville 3010, Australia
Microbiology 155:3868-80. 2009..Deletion of either the DNA-binding domain or the leucine zipper domain results in loss of both nitrogen and developmental phenotypes...
Sumoylation in Aspergillus nidulans: sumO inactivation, overexpression and live-cell imagingKoon Ho Wong
Department of Genetics, The University of Melbourne, Grattan Street, Parkville, VIC 3010, Australia
Fungal Genet Biol 45:728-37. 2008..Localization of GFP-SumO is dynamic, accumulating in punctate spots within the nucleus during interphase, lost at the onset of mitosis and re-accumulating during telophase...
Transcriptional control of nmrA by the bZIP transcription factor MeaB reveals a new level of nitrogen regulation in Aspergillus nidulansKoon Ho Wong
Department of Genetics, The University of Melbourne, Melbourne, VIC 3010, Australia
Mol Microbiol 66:534-51. 2007..Expression of meaB was not strongly regulated suggesting that transcriptional activation by MeaB is modulated by the nitrogen status. This work highlights a new level of complexity in the regulation of nitrogen catabolism...
TupA, the Penicillium marneffei Tup1p homologue, represses both yeast and spore developmentRichard B Todd
Department of Genetics, The University of Melbourne, 3010, Australia
Mol Microbiol 48:85-94. 2003..Therefore, TupA coordinates cell fate by promoting filamentation and repressing both spore and yeast morphogenetic programmes...
Detection of unpaired DNA at meiosis results in RNA-mediated silencingMichael J Hynes
Department of Genetics, University of Melbourne, Parkville, Australia
Bioessays 25:99-103. 2003..crassa and other organisms. Finally, this gene product is essential for normal meiosis, suggesting that RNA-dependent processes are fundamental to the sexual cycle...
Characterization of regulatory non-catalytic hexokinases in Aspergillus nidulansStella M H Bernardo
Molecular and Cellular Biology, University of New England, Armidale, NSW, Australia
Mol Genet Genomics 277:519-32. 2007..Homologs of hxkC and hxkD are conserved in multi-cellular fungi. Genes encoding atypical hexokinases are present in many genome sequence databases. Thus, non-catalytic hexokinases may be widespread...
