Research Topics
Genomes and GenesSpecies | P K GroverSummaryAffiliation: Flinders University Country: Australia Publications
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Publications
The effects of intracrystalline and surface-bound proteins on the attachment of calcium oxalate monohydrate crystals to renal cells in undiluted human urinePhulwinder K Grover
Urology Unit, Department of Surgery, School of Medicine, The Flinders University of South Australia, Bedford Park, South Australia, Australia
BJU Int 105:708-15. 2010....
Effect of urine fractionation on attachment of calcium oxalate crystals to renal epithelial cells: implications for studying renal calculogenesisPhulwinder K Grover
Urology Unit, Department of Surgery, Flinders Medical Centre, and Flinders University, South Australia, Australia
Am J Physiol Renal Physiol 292:F1396-403. 2007..Fractionation of human urine affects the attachment of COM crystals to MDCK cells. Hence future studies investigating regulation of crystal-cell interactions should be carried out in untreated urine as the binding medium...
Renal prothrombin mRNA is significantly decreased in a hyperoxaluric rat model of nephrolithiasisP K Grover
Urology Unit, Department of Surgery, Flinders Medical Centre and Flinders University Bedford Park 5042, South Australia, Australia
J Pathol 210:273-81. 2006..This is the first report of a significant reduction in the renal expression of a urinary protein well documented to inhibit CaOx crystal growth and aggregation in undiluted human urine in vitro...
Intracrystalline urinary proteins facilitate degradation and dissolution of calcium oxalate crystals in cultured renal cellsPhulwinder K Grover
Urology Unit, Department of Surgery, School of Medicine, Flinders University, Bedford Park, South Australia, Australia
Am J Physiol Renal Physiol 294:F355-61. 2008..The findings have significant ramifications in biomineral metabolism and pathogenesis of renal stones...
Critical appraisal of salting-out and its implications for chemical and biological sciencesPhulwinder K Grover
Division of Urology, Department of Surgery, Flinders University School of Medicine, Flinders Medical Centre, Bedford Park, South Australia 5042, Australia
Chem Rev 105:1-10. 2005
Renal prothrombin: quantification of mRNA using reverse transcription-polymerase chain reaction in a rat modelPhulwinder K Grover
Urology Unit, Department of Surgery, Flinders Medical Centre, Bedford Park, South Australia 5042, Australia
Electrophoresis 25:797-803. 2004..3669 (with a mean +/- SD of 0.3729 +/- 0.0716, range 0.2718-0.4675) in the hepatic tissues. Thus, expression of PT mRNA in the rat kidney was almost 300-fold less as compared with its expression in hepatic tissue...
Dissolved urate salts out calcium oxalate in undiluted human urine in vitro: implications for calcium oxalate stone genesisPhulwinder K Grover
Division of Urology, Department of Surgery, Flinders University School of Medicine, Flinders Medical Centre, Bedford Park, South Australia 5042, Australia
Chem Biol 10:271-8. 2003..We also outline anti-salting out strategies for future research for the prevention and/or treatment of CaOx calculi...
The effect of preincubation of seed crystals of uric acid and monosodium urate with undiluted human urine to induce precipitation of calcium oxalate in vitro : implications for urinary stone formationPhulwinder K Grover
Division of Urology, Department of Surgery, Flinders University School of Medicine, Flinders Medical Centre, Bedford Park, South Australia, AU
Mol Med 8:525-35. 2002....
The effect of seed crystals of hydroxyapatite and brushite on the crystallization of calcium oxalate in undiluted human urine in vitro: implications for urinary stone pathogenesisPhulwinder K Grover
Department of Surgery, Flinders University School of Medicine, Flinders Medical Centre, Bedford Park, South Australia, Australia
Mol Med 8:200-9. 2002....
Effect of prothrombin and its activation fragments on calcium oxalate crystal growth and aggregation in undiluted human urine in vitro: relationship between protein structure and inhibitory activityPhulwinder K Grover
Urology Unit, Department of Surgery, Flinders Medical Centre, Bedford Park, South Australia 5042, Australia
Clin Sci (Lond) 102:425-34. 2002..The superior potency of F1, compared with prothrombin, probably results from the molecule's greater charge-to-mass ratio...
High resolution two-dimensional electrophoretic analysis of urinary proteins of patients with prostatic cancerP K Grover
Department of Urology, Case Western Reserve University, School of Medicine, Cleveland, OH, USA
Electrophoresis 18:814-8. 1997..Hence, it may become useful in distinguishing BPH from PCA. All the proteins, A to F, appear to be previously unidentified. Their further characterization is warranted...
Inhibition of growth and aggregation of calcium oxalate crystals in vitro--a comparison of four human proteinsP K Grover
Department of Surgery, Flinders Medical Centre, Adelaide, South Australia, Australia
Eur J Biochem 253:637-44. 1998..Moreover, unlike those proteins it significantly inhibits the deposition of calcium oxalate. These findings have implications for the putative role of urinary proteins in the formation of calcium oxalate stones...
Inhibition of calcium oxalate crystal growth and aggregation by prothrombin and its fragments in vitro: relationship between protein structure and inhibitory activityP K Grover
Urology Unit, Department of Surgery, Flinders Medical Centre, Flinders University of South Australia
Eur J Biochem 263:50-6. 1999..The superior potency of F1, in comparison with PT, probably results from the molecule's greater charge to mass ratio...
Prothrombin gene expression in rat kidneys provides an opportunity to examine its role in urinary stone pathogenesisP K Grover
Urology Unit, Repatriation General Hospital, Daw Park, South Australia, Australia
J Am Soc Nephrol 10:S404-7. 1999..This similarity between human and rat kidneys allows the possibility of using established rat models of stone disease to evaluate therapeutic strategies to reduce stone formation...
Simple, sensitive and accurate method for the quantification of prothrombin mRNA by using competitive PCRP K Grover
Urology Unit, Flinders Medical Centre, Bedford Park 5042, South Australia, Australia
Biochem J 356:111-20. 2001..Although the method was developed for the quantification of PT transcripts in liver, it can easily be used for non-hepatic tissues as well. The technique is simple, quick and sensitive and requires only a very small amount of substrate...
The prothrombin gene is expressed in the rat kidney: Implications for urolithiasis researchP K Grover
Urology Unit, Flinders Medical Center, Bedford Park, South Australia, Australia
Eur J Biochem 267:61-7. 2000....
The hole truth: intracrystalline proteins and calcium oxalate kidney stonesR L Ryall
Department of Surgery, Flinders University School of Medicine, Flinders Medical Centre, Bedford Park, South Australia, Australia
Mol Urol 4:391-402. 2000..Understanding the basic molecular strategies by which plants produce protein-containing CaOx crystals may provide insight into human CaOx stone formation...
Face-specific binding of prothrombin fragment 1 and human serum albumin to inorganic and urinary calcium oxalate monohydrate crystalsAlison F Cook
Department of Surgery, Flinders University School of Medicine, Flinders Medical Centre, Bedford Park, South Australia, Australia
BJU Int 103:826-35. 2009..Accelrys Materials Studio and Discovery Studio were used to model the binding of PTF1 to the top, side and apical faces of the COM crystal...
Intracrystalline proteins and urolithiasis: a comparison of the protein content and ultrastructure of urinary calcium oxalate monohydrate and dihydrate crystalsRosemary L Ryall
Department of Surgery, Flinders Medical Centre and Flinders University School of Medicine, Bedford Park, South Australia
BJU Int 96:654-63. 2005....
The importance of a clean face: the effect of different washing procedures on the association of Tamm-Horsfall glycoprotein and other urinary proteins with calcium oxalate crystalsRosemary Lyons Ryall
Department of Surgery, Flinders Medical Centre, Flinders University School of Medicine, Bedford Park, SA, 5042, Australia
Urol Res 35:1-14. 2007....
High resolution 2-dimensional electrophoretic analysis of proteins in saline bladder washingsP J Sweeney
Department of Urology, Case Western Reserve University School of Medicine, Cleveland, Ohio
J Urol 152:2258-61. 1994..The 2-dimensional profiles we developed can be used as a data base to study urothelium derived proteins in various disease conditions such as bladder cancer...
High calcium concentration and calcium oxalate crystals cause significant inaccuracies in the measurement of urinary osteopontin by enzyme linked immunosorbent assayLauren A Thurgood
Department of Surgery, Flinders Medical Centre and Flinders University School of Medicine, Bedford Park, SA, 5042, Australia
Urol Res 36:103-10. 2008....
Intracrystalline proteins and urolithiasis: a synchrotron X-ray diffraction study of calcium oxalate monohydrateDavid E Fleming
Department of Applied Chemistry, Curtin University of Technology, Perth, Western Australia, Australia
J Bone Miner Res 18:1282-91. 2003..We therefore propose that intracrystalline proteins may constitute a natural form of defense against renal stone formation...
Allopurinol for stones: right drug--wrong reasonsPhulwinder K Grover
Am J Med 120:380. 2007
Comment on epitaxial relationships between uric acid crystals and mineral surfaces: a factor in urinary stone formationPhulwinder K Grover
Langmuir 21:10898. 2005
